Timme, Lukas Theodor; Henschen, Helena; Puerto Rueda, Diana N; Manda, Sneha; Parkinson, John Everett; Wild, Christian; Abramovich, Sigal; Morard, Raphael; Schmidt, Christiane: Menthol-DCMU bleaching and re-inoculation of Symbiodiniaceae in the large benthic foraminifera Sorites orbiculus [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.984033 (dataset in review)
Abstract:
Sorites orbiculus specimens were collected on 26 July 2023 from the Gulf of Aqaba at the Interuniversity Institute for Marine Sciences in Eilat. In a controlled laboratory setting at the Leibniz Institute for Tropical Marine Research MAREE facility, these specimens underwent a bleaching process using 0.19 mmol L-1 menthol and 5 µmol L-1 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) over 27 days. Post-bleaching, the specimens were re-inoculated for 19 days with the Symbiodiniaceae strains Symbiodinium microadriaticum (strains CCMP2467 and KB8) and Fugacium kawagutii (strain F2) at a concentration of 1 × 10⁵ cells mL⁻¹. Subsequently, the specimens were cultivated for an additional 37 days to assess the stability of the newly established symbiotic associations. Throughout the experiment, physiological parameters were monitored using confocal laser scanning microscopy, pulse-amplitude modulated fluorometry, standard microscopy, motility assessments, and real-time quantitative polymerase chain reaction to confirm symbiont identity.
Keyword(s):
Funding:
Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. 444059848: SYMBIOAID: the role of diatom endosymbionts on the adaptive potenital of benthic foraminifera to climate change
Coverage:
Latitude: 29.501812 * Longitude: 34.917251
Date/Time Start: 2023-06-26T00:00:00 * Date/Time End: 2023-11-08T00:00:00
Event(s):
S_orbiculus_2023 * Latitude: 29.501812 * Longitude: 34.917251 * Date/Time Start: 2023-06-26T00:00:00 * Date/Time End: 2023-06-26T00:00:00 * Location: Gulf of Aqaba * Method/Device: Sampling by snorkeling (SNORKELING) * Comment: sampling depth: 2 m; sample location: Inter-University Institute for Marine Sciences
Comment:
Symbiodinium microadriaticum, strain CCMP2467: collected in the Gulf of Aquaba on 2004-07-30, symbiont isolated from Stylophora pistillata
Symbiodinium microadriaticum, strain KB8: collected in Hawaii, symbiont isolated from Cassiopaia xamancha
Fugacium kawagutii, strain F2: collected in Jamaica, Caribbean, symbiont isolated from Meandrina meandrites
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Size:
11 datasets
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Datasets listed in this bundled publication
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): Confocal laser scanning microscopy monitoring bleaching in the foraminifera Sorites orbiculus. https://doi.pangaea.de/10.1594/PANGAEA.984034
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): Confocal laser scanning microscopy monitoring re-inoculation of Symbiodiniaceae in the foraminifera Sorites orbiculus. https://doi.pangaea.de/10.1594/PANGAEA.984035
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): Confocal laser scanning microscopy monitoring symbiosis stability of Symbiodiniaceae in the foraminifera Sorites orbiculus. https://doi.pangaea.de/10.1594/PANGAEA.984036
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): Growth rate of the foraminifera Sorites orbiculus during bleaching treatment. https://doi.pangaea.de/10.1594/PANGAEA.984037
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): Growth rate of the foraminifera Sorites orbiculus during Symbiodiniaceae re-inoculation treatment. https://doi.pangaea.de/10.1594/PANGAEA.984038
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): Growth rate of the foraminifera Sorites orbiculus during Symbiodiniaceae symbiosis stability observation. https://doi.pangaea.de/10.1594/PANGAEA.984039
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): PAM fluorometry monitoring bleaching in the foraminifera Sorites orbiculus. https://doi.pangaea.de/10.1594/PANGAEA.984041
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): PAM fluorometry monitoring re-inoculation of Symbiodiniaceae in the foraminifera Sorites orbiculus. https://doi.pangaea.de/10.1594/PANGAEA.984042
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): PAM fluorometry monitoring symbiosis stability of Symbiodiniaceae in the foraminifera Sorites orbiculus. https://doi.pangaea.de/10.1594/PANGAEA.984043
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): Motility of the foraminifera Sorites orbiculus during bleaching treatment. https://doi.pangaea.de/10.1594/PANGAEA.984040
- Timme, LT; Henschen, H; Puerto Rueda, DN et al. (in review): qPCR before and after bleaching and after symbiont switing in the foraminifera Sorites orbiculus. https://doi.pangaea.de/10.1594/PANGAEA.984044