Timme, Lukas Theodor; Henschen, Helena; Puerto Rueda, Diana N; Manda, Sneha; Parkinson, John Everett; Wild, Christian; Abramovich, Sigal; Morard, Raphael; Schmidt, Christiane: Confocal laser scanning microscopy monitoring symbiosis stability of Symbiodiniaceae in the foraminifera Sorites orbiculus [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.984036 (dataset in review), In: Timme, LT et al.: Menthol-DCMU bleaching and re-inoculation of Symbiodiniaceae in the large benthic foraminifera Sorites orbiculus [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.984033 (dataset in review)
Abstract:
The long-term stability of newly established symbiotic associations in Sorites orbiculus specimens was assessed with Confocal Laser Scanning Microscopy (CLSM), measuring the percentage of host area covered with symbionts, in a 14-day cultivation following menthol-DCMU bleaching and re-inoculation with Symbiodinium microadriaticum (strains KB8 and CCMP2467) and Fugacium kawagutii (strain F2) individually and collectively. The final re-inoculation measurement also served as the initial stability measurement. Accordingly, this measurement is included in both data tables and is provided here under the experimental treatment label "re-inoculation." Specimens were cultivated in 6-well plates, with 2 samples containing 2 replicates per treatment / control. The medium, supplemented with a Nannochloropsis food mixture, was exchanged twice weekly. Control groups included non-bleached specimens and bleached specimens maintained with or without 5 µmol L-1 DCMU in artificial seawater (ASW). Environmental conditions were maintained at 22 - 24°C temperature, 37.5 - 40.5‰ salinity, and 46 - 63 µmol photons cm-2 s-1 illumination under a 12:12 h light-dark cycle.
Funding:
Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. 444059848: SYMBIOAID: the role of diatom endosymbionts on the adaptive potenital of benthic foraminifera to climate change
Coverage:
Latitude: 29.501812 * Longitude: 34.917251
Date/Time Start: 2023-10-23T00:00:00 * Date/Time End: 2023-11-06T00:00:00
Event(s):
S_orbiculus_2023 * Latitude: 29.501812 * Longitude: 34.917251 * Date/Time Start: 2023-06-26T00:00:00 * Date/Time End: 2023-06-26T00:00:00 * Location: Gulf of Aqaba * Method/Device: Sampling by snorkeling (SNORKELING) * Comment: sampling depth: 2 m; sample location: Inter-University Institute for Marine Sciences
Comment:
Symbiodinium microadriaticum, strain CCMP2467: collected in the Gulf of Aquaba on 2004-07-30, symbiont isolated from Stylophora pistillata
Symbiodinium microadriaticum, strain KB8: collected in Hawaii, symbiont isolated from Cassiopaia xamancha
Fugacium kawagutii, strain F2: collected in Jamaica, Caribbean, symbiont isolated from Meandrina meandrites
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experimental treatment | Exp treat | Timme, Lukas Theodor | |||
| 2 | Type of study | Study type | Timme, Lukas Theodor | |||
| 3 | DATE/TIME | Date/Time | Timme, Lukas Theodor | Geocode | ||
| 4 | Method comment | Method comm | Timme, Lukas Theodor | |||
| 5 | Method comment | Method comm | Timme, Lukas Theodor | short | ||
| 6 | Sample ID | Sample ID | Timme, Lukas Theodor | |||
| 7 | Specimen number | Spec no | Timme, Lukas Theodor | |||
| 8 | Area | Area | µm2 | Timme, Lukas Theodor | Sorites | |
| 9 | Area | Area | µm2 | Timme, Lukas Theodor | Symbionts | |
| 10 | Coverage | Cov | % | Timme, Lukas Theodor | Percentage of host area covered with symbionts |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Size:
756 data points