Timme, Lukas Theodor; Henschen, Helena; Puerto Rueda, Diana N; Manda, Sneha; Parkinson, John Everett; Wild, Christian; Abramovich, Sigal; Morard, Raphael; Schmidt, Christiane: Confocal laser scanning microscopy monitoring bleaching in the foraminifera Sorites orbiculus [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.984034 (dataset in review), In: Timme, LT et al.: Menthol-DCMU bleaching and re-inoculation of Symbiodiniaceae in the large benthic foraminifera Sorites orbiculus [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.984033 (dataset in review)
Abstract:
Sorites orbiculus specimens were subjected to a bleaching treatment in a laboratory experiment. The percentage of host area covered with symbionts was monitored using Confocal Laser Scanning Microscopy (CLSM) with 5 samples containing 2 replicates per control / treatment. The treatment involved exposure to 5 µmol L-1 DCMU continuously and 0.19 mmol L-1 menthol during 6-hour incubation periods per day for 5 days per week over 27 days. Specimens were maintained in 120 ml of artificial seawater, with full water exchanges conducted twice daily, five days per week. In the final three days, specimens were incubated in the dark with only DCMU present. During the 18 h incubation periods, the weekends and the last three days, a Nannochloropsis food mixture was added. Control groups underwent identical conditions without the addition of bleaching agents. Environmental parameters were maintained at 26°C temperature, 37.5‰ salinity, and an illumination of 48 µmol photons cm-2 s-1 under a 12:12 h light-dark cycle.
Funding:
Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. 444059848: SYMBIOAID: the role of diatom endosymbionts on the adaptive potenital of benthic foraminifera to climate change
Coverage:
Latitude: 29.501812 * Longitude: 34.917251
Date/Time Start: 2023-08-28T00:00:00 * Date/Time End: 2023-09-26T00:00:00
Event(s):
S_orbiculus_2023 * Latitude: 29.501812 * Longitude: 34.917251 * Date/Time Start: 2023-06-26T00:00:00 * Date/Time End: 2023-06-26T00:00:00 * Location: Gulf of Aqaba * Method/Device: Sampling by snorkeling (SNORKELING) * Comment: sampling depth: 2 m; sample location: Inter-University Institute for Marine Sciences
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experimental treatment | Exp treat | Timme, Lukas Theodor | |||
| 2 | Type of study | Study type | Timme, Lukas Theodor | |||
| 3 | DATE/TIME | Date/Time | Timme, Lukas Theodor | Geocode | ||
| 4 | Method comment | Method comm | Timme, Lukas Theodor | |||
| 5 | Method comment | Method comm | Timme, Lukas Theodor | short | ||
| 6 | Sample ID | Sample ID | Timme, Lukas Theodor | |||
| 7 | Specimen number | Spec no | Timme, Lukas Theodor | |||
| 8 | Area | Area | µm2 | Timme, Lukas Theodor | Sorites | |
| 9 | Area | Area | µm2 | Timme, Lukas Theodor | Symbionts | |
| 10 | Coverage | Cov | % | Timme, Lukas Theodor | Percentage of host area covered with symbionts |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Size:
792 data points