Data Description

Citation:
Engel, A et al. (2015): No detectable effect of CO2 on elemental stoichiometry of Emiliania huxleyi in nutrient-limited, acclimated continuous cultures. doi:10.1594/PANGAEA.845818,
Supplement to: Engel, Anja; Cisternas Novoa, Carolina; Wurst, Mascha; Endres, Sonja; Tang, Tiantian; Schartau, Markus; Lee, Cindy (2014): No detectable effect of CO2 on elemental stoichiometry of Emiliania huxleyi in nutrient-limited, acclimated continuous cultures. Marine Ecology Progress Series, 507, 15-30, doi:10.3354/meps10824
Abstract:
Effects of CO2 concentration on elemental composition of the coccolithophore Emiliania huxleyi were studied in phosphorus-limited, continuous cultures that were acclimated to experimental conditions for 30 d prior to the first sampling. We determined phytoplankton and bacterial cell numbers, nutrients, particulate components like organic carbon (POC), inorganic carbon (PIC), nitrogen (PN), organic phosphorus (POP), transparent exopolymer particles (TEP), as well as dissolved organic carbon (DOC) and nitrogen (DON), in addition to carbonate system parameters at CO2 levels of 180, 380 and 750 µatm. No significant difference between treatments was observed for any of the measured variables during repeated sampling over a 14 d period. We considered several factors that might lead to these results, i.e. light, nutrients, carbon overconsumption and transient versus steady-state growth. We suggest that the absence of a clear CO2 effect during this study does not necessarily imply the absence of an effect in nature. Instead, the sensitivity of the cell towards environmental stressors such as CO2 may vary depending on whether growth conditions are transient or sufficiently stable to allow for optimal allocation of energy and resources. We tested this idea on previously published data sets where PIC and POC divided by the corresponding cell abundance of E. huxleyi at various pCO2 levels and growth rates were available.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb *
Project(s):
Comment:
This work was partly financed by ADAGIO http://www.somassbu.org/research/adagio/
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-04-20.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Species *SpeciesEngel, Anja *
2Figure *FigEngel, Anja *
3Day of experiment *DOEdayEngel, Anja *
4Carbon dioxide, partial pressure *pCO2µatmEngel, Anja *
5Abundance per volume *Abund v#/mlEngel, Anja *
6Emiliania huxleyi *E. huxleyi#/lEngel, Anja *10**8
7Emiliania huxleyi, standard deviation *E. huxleyi std dev±Engel, Anja *10**8
8Chlorophyll a per cell *Chl apg/#Engel, Anja *
9Chlorophyll a, standard deviation *Chl a std dev±Engel, Anja *
10Carbon, organic, particulate, per cell *POC/cellpmol/#Engel, Anja *
11Carbon, organic, particulate, per volume, standard deviation *POC std dev±Engel, Anja *
12Carbon, inorganic, particulate, per cell *PIC/cellpmol/#Engel, Anja *
13Standard deviation *Std dev±Engel, Anja *PIC/cell
14Nitrogen, particulate, per cell *PN/cellpmol/#Engel, Anja *
15Standard deviation *Std dev±Engel, Anja *PN/cell
16Phosphorus, organic, particulate, per cell *POP/cellpmol/#Engel, Anja *
17Standard deviation *Std dev±Engel, Anja *POP/cell
18Carbon, organic, dissolved, per cell *DOC/cellpmol/#Engel, Anja *
19Standard deviation *Std dev±Engel, Anja *DOC/cell
20Nitrogen, organic, dissolved, per cell *DON/cellpmol/#Engel, Anja *
21Standard deviation *Std dev±Engel, Anja *DON/cell
22Particulate inorganic carbon/particulate organic carbon ratio *PIC/POC ratioEngel, Anja *
23Particulate inorganic carbon/particulate organic carbon ratio, standard deviation *PIC/POC ratio std dev±Engel, Anja *
24Carbon, organic, particulate/Nitrogen, particulate ratio *POC/PNEngel, Anja *
25Carbon, organic, particulate/Nitrogen, particulate ratio, standard deviation *POC/PN std dev±Engel, Anja *
26Nitrogen, total particulate/Phosphorus, organic particulate ratio *TPN/POPEngel, Anja *
27Nitrogen, total particulate/Phosphorus, organic particulate, ratio, standard deviation *TPN/POP std dev±Engel, Anja *
28Carbon, organic, dissolved/Nitrogen, organic, dissolved ratio *DOC/DONEngel, Anja *
29Standard deviation *Std dev±Engel, Anja *DOC/DON
30Salinity *SalEngel, Anja *
31Temperature, water *Temp°CEngel, Anja *
32pH *pHEngel, Anja *NBS scale
33pH, standard deviation *pH std dev±Engel, Anja *NBS scale
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmEngel, Anja *
35Partial pressure of carbon dioxide, standard deviation *pCO2 std dev±Engel, Anja *
36Calcite saturation state *Omega CalEngel, Anja *
37Calcite saturation state, standard deviation *Omega Cal std dev±Engel, Anja *
38Alkalinity, total *ATµmol/kgEngel, Anja *
39Alkalinity, total, standard deviation *AT std dev±Engel, Anja *
40Carbonate system computation flag *CSC flagYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
41pH *pHYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *total scale
42Carbon dioxide *CO2µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
43Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
44Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
45Bicarbonate ion *[HCO3]-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
46Carbonate ion *[CO3]2-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
47Carbon, inorganic, dissolved *DICµmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
48Aragonite saturation state *Omega ArgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
49Calcite saturation state *Omega CalYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
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