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Engel, Anja; Cisternas Novoa, Carolina; Wurst, Mascha; Endres, Sonja; Tang, Tiantian; Schartau, Markus; Lee, Cindy (2014): No detectable effect of CO2 on elemental stoichiometry of Emiliania huxleyi in nutrient-limited, acclimated continuous cultures [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.845818, Supplement to: Engel, A et al. (2014): No detectable effect of CO2 on elemental stoichiometry of Emiliania huxleyi in nutrient-limited, acclimated continuous cultures. Marine Ecology Progress Series, 507, 15-30, https://doi.org/10.3354/meps10824

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Published: 2014 (exact date unknown) • DOI registered: 2015-05-28

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Abstract:
Effects of CO2 concentration on elemental composition of the coccolithophore Emiliania huxleyi were studied in phosphorus-limited, continuous cultures that were acclimated to experimental conditions for 30 d prior to the first sampling. We determined phytoplankton and bacterial cell numbers, nutrients, particulate components like organic carbon (POC), inorganic carbon (PIC), nitrogen (PN), organic phosphorus (POP), transparent exopolymer particles (TEP), as well as dissolved organic carbon (DOC) and nitrogen (DON), in addition to carbonate system parameters at CO2 levels of 180, 380 and 750 µatm. No significant difference between treatments was observed for any of the measured variables during repeated sampling over a 14 d period. We considered several factors that might lead to these results, i.e. light, nutrients, carbon overconsumption and transient versus steady-state growth. We suggest that the absence of a clear CO2 effect during this study does not necessarily imply the absence of an effect in nature. Instead, the sensitivity of the cell towards environmental stressors such as CO2 may vary depending on whether growth conditions are transient or sufficiently stable to allow for optimal allocation of energy and resources. We tested this idea on previously published data sets where PIC and POC divided by the corresponding cell abundance of E. huxleyi at various pCO2 levels and growth rates were available.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb
Project(s):
Biological Impacts of Ocean Acidification (BIOACID)
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
This work was partly financed by ADAGIO http://www.somassbu.org/research/adagio/
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-04-20.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesEngel, Anja
2FigureFigEngel, Anja
3Day of experimentDOEdayEngel, Anja
4Carbon dioxide, partial pressurepCO2µatmEngel, Anja
5Abundance per volumeAbund v#/mlEngel, Anja
6Emiliania huxleyiE. huxleyi#/lEngel, Anja10**8
7Emiliania huxleyi, standard deviationE. huxleyi std dev±Engel, Anja10**8
8Chlorophyll a per cellChl a/cellpg/#Engel, Anja
9Chlorophyll a, standard deviationChl a std dev±Engel, Anja
10Carbon, organic, particulate, per cellPOC/cellpmol/#Engel, Anja
11Carbon, organic, particulate, standard deviationPOC std dev±Engel, Anja
12Carbon, inorganic, particulate, per cellPIC/cellpmol/#Engel, Anja
13Standard deviationStd dev±Engel, AnjaPIC/cell
14Nitrogen, particulate, per cellPN/cellpmol/#Engel, Anja
15Standard deviationStd dev±Engel, AnjaPN/cell
16Phosphorus, organic, particulate, per cellPOP/cellpmol/#Engel, Anja
17Standard deviationStd dev±Engel, AnjaPOP/cell
18Carbon, organic, dissolved, per cellDOC/cellpmol/#Engel, Anja
19Standard deviationStd dev±Engel, AnjaDOC/cell
20Nitrogen, organic, dissolved, per cellDON/cellpmol/#Engel, Anja
21Standard deviationStd dev±Engel, AnjaDON/cell
22Particulate inorganic carbon/particulate organic carbon ratioPIC/POCEngel, Anja
23Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Engel, Anja
24Carbon, organic, particulate/Nitrogen, particulate ratioPOC/PNEngel, Anja
25Carbon, organic, particulate/Nitrogen, particulate ratio, standard deviationPOC/PN std dev±Engel, Anja
26Nitrogen, total, particulate/Phosphorus, organic, particulate, ratioTPN/POPEngel, Anja
27Nitrogen, total, particulate/Phosphorus, organic, particulate, ratio, standard deviationTPN/POP std dev±Engel, Anja
28Carbon, organic, dissolved/Nitrogen, organic, dissolved ratioDOC/DONEngel, Anja
29Standard deviationStd dev±Engel, AnjaDOC/DON
30SalinitySalEngel, Anja
31Temperature, waterTemp°CEngel, Anja
32pH, NBS scalepH NBSEngel, AnjaNBS scale
33pH, standard deviationpH std dev±Engel, AnjaNBS scale
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmEngel, Anja
35Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Engel, Anja
36Calcite saturation stateOmega CalEngel, Anja
37Calcite saturation state, standard deviationOmega Cal std dev±Engel, Anja
38Alkalinity, totalATµmol/kgEngel, Anja
39Alkalinity, total, standard deviationAT std dev±Engel, Anja
40Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41pH, total scalepHTYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
42Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
46Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
47Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
48Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3723 data points

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