Citation:

meps10824

Name: No detectable effect of CO2 on elemental stoichiometry of Emiliania huxleyi in nutrient-limited, acclimated continuous cultures
Published: 2014
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species

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Abstract:

Effects of CO2 concentration on elemental composition of the coccolithophore Emiliania huxleyi were studied in phosphorus-limited, continuous cultures that were acclimated to experimental conditions for 30 d prior to the first sampling. We determined phytoplankton and bacterial cell numbers, nutrients, particulate components like organic carbon (POC), inorganic carbon (PIC), nitrogen (PN), organic phosphorus (POP), transparent exopolymer particles (TEP), as well as dissolved organic carbon (DOC) and nitrogen (DON), in addition to carbonate system parameters at CO2 levels of 180, 380 and 750 µatm. No significant difference between treatments was observed for any of the measured variables during repeated sampling over a 14 d period. We considered several factors that might lead to these results, i.e. light, nutrients, carbon overconsumption and transient versus steady-state growth. We suggest that the absence of a clear CO2 effect during this study does not necessarily imply the absence of an effect in nature. Instead, the sensitivity of the cell towards environmental stressors such as CO2 may vary depending on whether growth conditions are transient or sufficiently stable to allow for optimal allocation of energy and resources. We tested this idea on previously published data sets where PIC and POC divided by the corresponding cell abundance of E. huxleyi at various pCO2 levels and growth rates were available.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

This work was partly financed by ADAGIO http://www.somassbu.org/research/adagio/

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-04-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Engel, Anja
2	Figure	Fig		Engel, Anja
3	Day of experiment	DOE	day	Engel, Anja
4	Carbon dioxide, partial pressure	pCO2	µatm	Engel, Anja
5	Abundance per volume	Abund v	#/ml	Engel, Anja
6	Emiliania huxleyi	E. huxleyi	#/l	Engel, Anja		10**8
7	Emiliania huxleyi, standard deviation	E. huxleyi std dev	±	Engel, Anja		10**8
8	Chlorophyll a per cell	Chl a/cell	pg/#	Engel, Anja
9	Chlorophyll a, standard deviation	Chl a std dev	±	Engel, Anja
10	Carbon, organic, particulate, per cell	POC/cell	pmol/#	Engel, Anja
11	Carbon, organic, particulate, standard deviation	POC std dev	±	Engel, Anja
12	Carbon, inorganic, particulate, per cell	PIC/cell	pmol/#	Engel, Anja
13	Standard deviation	Std dev	±	Engel, Anja		PIC/cell
14	Nitrogen, particulate, per cell	PN/cell	pmol/#	Engel, Anja
15	Standard deviation	Std dev	±	Engel, Anja		PN/cell
16	Phosphorus, organic, particulate, per cell	POP/cell	pmol/#	Engel, Anja
17	Standard deviation	Std dev	±	Engel, Anja		POP/cell
18	Carbon, organic, dissolved, per cell	DOC/cell	pmol/#	Engel, Anja
19	Standard deviation	Std dev	±	Engel, Anja		DOC/cell
20	Nitrogen, organic, dissolved, per cell	DON/cell	pmol/#	Engel, Anja
21	Standard deviation	Std dev	±	Engel, Anja		DON/cell
22	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Engel, Anja
23	Particulate inorganic carbon/particulate organic carbon ratio, standard deviation	PIC/POC ratio std dev	±	Engel, Anja
24	Carbon, organic, particulate/Nitrogen, particulate ratio	POC/PN		Engel, Anja
25	Carbon, organic, particulate/Nitrogen, particulate ratio, standard deviation	POC/PN std dev	±	Engel, Anja
26	Nitrogen, total, particulate/Phosphorus, organic, particulate, ratio	TPN/POP		Engel, Anja
27	Nitrogen, total, particulate/Phosphorus, organic, particulate, ratio, standard deviation	TPN/POP std dev	±	Engel, Anja
28	Carbon, organic, dissolved/Nitrogen, organic, dissolved ratio	DOC/DON		Engel, Anja
29	Standard deviation	Std dev	±	Engel, Anja		DOC/DON
30	Salinity	Sal		Engel, Anja
31	Temperature, water	Temp	°C	Engel, Anja
32	pH	pH		Engel, Anja		NBS scale
33	pH, standard deviation	pH std dev	±	Engel, Anja		NBS scale
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Engel, Anja
35	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Engel, Anja
36	Calcite saturation state	Omega Cal		Engel, Anja
37	Calcite saturation state, standard deviation	Omega Cal std dev	±	Engel, Anja
38	Alkalinity, total	AT	µmol/kg	Engel, Anja
39	Alkalinity, total, standard deviation	AT std dev	±	Engel, Anja
40	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
42	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3723 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Fig	3 DOE [day]	4 pCO2 [µatm]	5 Abund v [#/ml]	6 E. huxleyi [#/l] (10**8)	7 E. huxleyi std dev [±] (10**8)	8 Chl a/cell [pg/#]	9 Chl a std dev [±]	10 POC/cell [pmol/#]	11 POC std dev [±]	12 PIC/cell [pmol/#]	13 Std dev [±] (PIC/cell)	14 PN/cell [pmol/#]	15 Std dev [±] (PN/cell)	16 POP/cell [pmol/#]	17 Std dev [±] (POP/cell)	18 DOC/cell [pmol/#]	19 Std dev [±] (DOC/cell)	20 DON/cell [pmol/#]	21 Std dev [±] (DON/cell)	22 PIC/POC	23 PIC/POC ratio std dev [±]	24 POC/PN	25 POC/PN std dev [±]	26 TPN/POP	27 TPN/POP std dev [±]	28 DOC/DON	29 Std dev [±] (DOC/DON)	30 Sal	31 Temp [°C]	32 pH (NBS scale)	33 pH std dev [±] (NBS scale)	34 pCO2water_SST_wet [µatm]	35 pCO2 std dev [±]	36 Omega Cal	37 Omega Cal std dev [±]	38 AT [µmol/kg]	39 AT std dev [±]	40 CSC flag	41 pH (total scale)	42 CO2 [µmol/kg]	43 fCO2water_SST_wet [µatm]	44 pCO2water_SST_wet [µatm]	45 [HCO3]- [µmol/kg]	46 [CO3]2- [µmol/kg]	47 DIC [µmol/kg]	48 Omega Arg	49 Omega Cal
Emiliania huxleyi (phytoplankton)	1	14	380	49732																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	15	380	89480																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	16	380	135600																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	17	380	158700																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	18	380	206000																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	19	380	251200																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	20	380	290700																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	21	380	323600																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	22	380	292100																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	23	380	327200																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	24	380	397600																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	25	380	449900																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	26	380	499200																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	27	380	531100																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	28	380	546400																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	29	380	537400																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	30	380	569400																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	31	380	466000																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	32	380	521700																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	33	380	559900																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	34	380	627400																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	35	380	511800																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	36	380	544700																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	37	380	589700																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	38	380	496300																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	40	380	463000																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	41	380	575300																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	14	180	5800																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	15	180	6158																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	16	180	11612																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	17	180	33094																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	18	180	80952																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	19	180	148400																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	20	180	186600																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	21	180	300900																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	22	180	299700																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	23	180	332400																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	24	180	356700																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	25	180	356500																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	26	180	451800																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	27	180	468100																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	28	180	443000																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	29	180	416700																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	30	180	451300																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	31	180	370600																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	32	180	420100																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	33	180	479600																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	34	180	520500																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	35	180	415500																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	36	180	479100																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	37	180	514400																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	38	180	448300																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	40	180	488000																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	41	180	481400																									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	1	14	750	6776																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	15	750	5336																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	16	750	4594																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	17	750	4656																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	18	750	5124																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	19	750	5142																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	20	750	6838																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	21	750	12020																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	22	750	18072																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	23	750	32026																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	24	750	63570																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	25	750	119300																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	26	750	207100																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	27	750	302800																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	28	750	372500																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	29	750	392200																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	30	750	445300																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	31	750	351700																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	32	750	430700																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	33	750	489700																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	34	750	535400																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	35	750	395800																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	36	750	513600																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	37	750	553700																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	38	750	502400																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	40	750	488100																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	41	750	562900																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	14	380	9624																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	15	380	8056																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	16	380	7010																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	17	380	6962																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	18	380	6896																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	19	380	7642																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	20	380	9862																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	21	380	20562																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	22	380	31058																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	23	380	54592																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	24	380	109200																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	25	380	190200																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	26	380	261200																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	27	380	329800																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	28	380	317000																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	29	380	385300																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	30	380	373100																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	31	380	345700																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	32	380	379600																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	33	380	419800																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	34	380	490300																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	35	380	399000																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	36	380	451300																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	37	380	501900																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	38	380	457300																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	40	380	491300																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	41	380	523200																									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	1	14	750	39296																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	15	750	59448																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	16	750	86290																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	17	750	108800																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	18	750	152500																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	19	750	212500																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	20	750	197200																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	21	750	207700																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	22	750	147200																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	23	750	212900																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	24	750	304200																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	25	750	370000																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	26	750	414400																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	27	750	480800																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	28	750	496900																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	29	750	482400																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	30	750	528700																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	31	750	431400																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	32	750	501800																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	33	750	521400																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	34	750	643700																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	35	750	493700																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	36	750	574400																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	37	750	601400																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	38	750	494500																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	40	750	531600																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	1	41	750	589400																									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	2+3		180		4.7	0.77	52.4071	16.0086																					33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	2+3		380		5.2	0.65	56.2522	17.7920																					33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	2+3		750		5.1	1.40	50.4149	21.2061																					33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	4		180						0.8184	0.0415	1.2618	0.0967	0.0516	0.0121	0.003	0.0010													33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	4		380						0.8033	0.0791	1.4745	0.3119	0.0534	0.0183	0.003	0.0010													33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	4		750						0.7867	0.0727	1.2754	0.1863	0.0482	0.0135	0.003	0.0009													33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	5		180														190	6.5600	22.5	0.6000									33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	5		380														190	5.5100	22.2	0.6000									33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	5		750														190	14.0000	22.3	0.7000									33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86
Emiliania huxleyi (phytoplankton)	6		180																		1.54	0.15	16.10	2.9	17.7209	8.1184	8.4	0.5600	33	14	8.50	0.06	183	58	6.36	0.42	2400	206	26	8.39	6.38	163.31	164	1708.42	283.17	1997.97	4.37	6.84
Emiliania huxleyi (phytoplankton)	6		380																		1.79	0.38	16.57	3.0	19.6551	5.7991	8.56	0.3500	33	14	8.23	0.05	357	74	4.27	0.49	2473	226	26	8.12	14.14	361.95	363	2033.16	180.96	2228.26	2.79	4.37
Emiliania huxleyi (phytoplankton)	6		750																		1.61	0.23	17.10	3.5	18.0018	6.8034	8.5	0.7100	33	14	8.02	0.06	616	104	2.83	0.57	2450	255	26	7.91	24.38	624.09	626	2161.14	118.58	2304.09	1.83	2.86