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Rouco, Mónica; Branson, O; Lebrato, Mario; Iglesias-Rodriguez, Debora (2013): The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios. doi:10.1594/PANGAEA.842157,
Supplement to: Rouco, M et al. (2013): The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios. Frontiers in Microbiology, 4, doi:10.3389/fmicb.2013.00155

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Abstract:
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 µatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 ?atm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-01-29.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesRouco, Mónica
2TableTabRouco, Mónica
3FigureFigRouco, Mónica
4Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmRouco, Mónicatreatment
5TreatmentTreatRouco, Mónica
6Nitrate reductase activity, per total proteinNO3 reduct actUEA/#Rouco, Mónica
7Alkaline phosphatase, para-Nitrophenylphosphate per cellALKP pNPP/cellnmol/min/#Rouco, Mónica
8Particulate organic carbon content per cellPOC contpmol/#Rouco, Mónica
9Particulate organic carbon content per cell, standard deviationPOC cont std dev±Rouco, Mónica
10Particulate organic nitrogen per cellPON cellpmol/#Rouco, Mónica
11Particulate organic nitrogen per cell, standard deviationPON cell std dev±Rouco, Mónica
12Particulate inorganic carbon per cellPIC cellpmol/#Rouco, Mónica
13Particulate inorganic carbon per cell, standard deviationPIC in cell std dev±Rouco, Mónica
14Particulate organic phosphorus per cellPOP cellpmol/#Rouco, Mónica
15Particulate organic phosphorus per cell, standard deviationPOP cell std dev±Rouco, Mónica
16Particulate inorganic carbon/particulate organic carbon ratioPIC/POC ratioRouco, Mónica
17Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Rouco, Mónica
18Coccoliths, volumeCocc volµm3Rouco, Mónica
19Coccoliths, volume, standard deviationCocc vol std dev±Rouco, Mónica
20Coccosphere, lengthCoccosp lµmRouco, Mónica
21Coccosphere, length, standard deviationCoccosp l std dev±Rouco, Mónica
22Temperature, waterTemp°CRouco, Mónica
23Temperature, water, standard deviationTemp std dev±Rouco, Mónica
24SalinitySalRouco, Mónica
25Trientalis-typeTre-T#Rouco, Mónica
26IrradianceEµE/m2/sRouco, Mónica
27Irradiance, standard deviationE std dev±Rouco, Mónica
28NitrateNITRATµmol/kgRouco, Mónicaat the end of the experiment
29Nitrate, standard deviationNO3 std dev±Rouco, Mónicaat the end of the experiment
30PhosphatePHSPHTµmol/kgRouco, Mónicaat the end of the experiment
31Phosphate, standard deviationPO4 std dev±Rouco, Mónicaat the end of the experiment
32Carbon, inorganic, dissolvedDICµmol/kgRouco, MónicaCoulometric titrationat the end of the experiment
33Carbon, inorganic, dissolved, standard deviationDIC std dev±Rouco, MónicaCoulometric titrationat the end of the experiment
34Alkalinity, totalATµmol/kgRouco, MónicaPotentiometric titrationat the end of the experiment
35Alkalinity, total, standard deviationAT std dev±Rouco, MónicaPotentiometric titrationat the end of the experiment
36pHpHRouco, MónicaCalculated using CO2SYStotal scale, at the end of the experiment
37pH, standard deviationpH std dev±Rouco, MónicaCalculated using CO2SYStotal scale, at the end of the experiment
38Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmRouco, MónicaCalculated using CO2SYSat the end of the experiment
39Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Rouco, MónicaCalculated using CO2SYSat the end of the experiment
40Bicarbonate ion[HCO3]-µmol/kgRouco, MónicaCalculated using CO2SYSat the end of the experiment
41Bicarbonate, standard deviation[HCO3]- std dev±Rouco, MónicaCalculated using CO2SYSat the end of the experiment
42Carbonate ion[CO3]2-µmol/kgRouco, MónicaCalculated using CO2SYSat the end of the experiment
43Carbonate ion, standard deviation[CO3]2- std dev±Rouco, MónicaCalculated using CO2SYSat the end of the experiment
44Carbon dioxideCO2µmol/kgRouco, MónicaCalculated using CO2SYSat the end of the experiment
45Carbon dioxide, standard deviationCO2 std dev±Rouco, MónicaCalculated using CO2SYSat the end of the experiment
46Calcite saturation stateOmega CalRouco, MónicaCalculated using CO2SYSat the end of the experiment
47Calcite saturation state, standard deviationOmega Cal std dev±Rouco, MónicaCalculated using CO2SYSat the end of the experiment
48Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
50Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
51Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
53Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
54Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
55Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
56Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
1422 data points

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