Data Description

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Citation:
Rouco, M et al. (2013): The effect of nitrate and phosphate availability on Emiliania huxleyi(NZEH) physiology under different CO2 scenarios. doi:10.1594/PANGAEA.842157,
Supplement to: Rouco, Mónica; Branson, O; Lebrato, Mario; Iglesias-Rodriguez, Debora (2013): The effect of nitrate and phosphate availability on Emiliania huxleyi (NZEH) physiology under different CO2 scenarios. Frontiers in Microbiology, 4, doi:10.3389/fmicb.2013.00155
Abstract:
Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 µatm) under three nutrient conditions [nutrient replete (R), nitrate limited (-N), and phosphate limited (-P)]. We focused on calcite and organic carbon quotas and on nitrate and phosphate utilization by analyzing the activity of nitrate reductase (NRase) and alkaline phosphatase (APase), respectively. Particulate inorganic (PIC) and organic (POC) carbon quotas increased with increasing CO2 under R conditions but a different pattern was observed under nutrient limitation. The PIC:POC ratio decreased with increasing CO2 in nutrient limited cultures. Coccolith length increased with CO2 under all nutrient conditions but the coccosphere volume varied depending on the nutrient treatment. Maximum APase activity was found at 561 ?atm of CO2 (pH 7.92) in -P cultures and in R conditions, NRase activity increased linearly with CO2. These results suggest that E. huxleyi's competitive ability for nutrient uptake might be altered in future high-CO2 oceans. The combined dataset will be useful in model parameterizations of the carbon cycle and ocean acidification.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb *
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-01-29.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Species *SpeciesRouco, Mónica *
2Table *TabRouco, Mónica *
3Figure *FigRouco, Mónica *
4Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmRouco, Mónica *treatment
5Treatment *TreatmRouco, Mónica *
6Nitrate reductase activity, per total protein *NO3 reduct actUEA/#Rouco, Mónica *
7Alkaline phosphatase, para-Nitrophenylphosphate per cell *ALKP pNPP/cellnmol/min/#Rouco, Mónica *
8Particulate organic carbon content per cell *POC contpmol/#Rouco, Mónica *
9Particulate organic carbon content per cell, standard deviation *POC cont std dev±Rouco, Mónica *
10Particulate organic nitrogen per cell *PON cellpmol/#Rouco, Mónica *
11Particulate organic nitrogen per cell, standard deviation *PON cell std dev±Rouco, Mónica *
12Particulate inorganic carbon per cell *PIC cellpmol/#Rouco, Mónica *
13Particulate inorganic carbon per cell, standard deviation *PIC in cell std dev±Rouco, Mónica *
14Particulate organic phosphorus per cell *POP cellpmol/#Rouco, Mónica *
15Particulate organic phosphorus per cell, standard deviation *POP cell std dev±Rouco, Mónica *
16Particulate inorganic carbon/particulate organic carbon ratio *PIC/POC ratioRouco, Mónica *
17Particulate inorganic carbon/particulate organic carbon ratio, standard deviation *PIC/POC ratio std dev±Rouco, Mónica *
18Coccoliths, volume *Cocc volµm3Rouco, Mónica *
19Coccoliths, volume, standard deviation *Cocc vol std dev±Rouco, Mónica *
20Coccosphere, length *Coccosp lµmRouco, Mónica *
21Coccosphere, length, standard deviation *Coccosp l std dev±Rouco, Mónica *
22Temperature, water *Temp°CRouco, Mónica *
23Temperature, water, standard deviation *Temp std dev±Rouco, Mónica *
24Salinity *SalRouco, Mónica *
25Trientalis-type *Tre-T#Rouco, Mónica *
26Irradiance *EµE/m2/sRouco, Mónica *
27Irradiance, standard deviation *E std dev±Rouco, Mónica *
28Nitrate *NITRATµmol/kgRouco, Mónica *at the end of the experiment
29Nitrate, standard deviation *NO3 std dev±Rouco, Mónica *at the end of the experiment
30Phosphate *PHSPHTµmol/kgRouco, Mónica *at the end of the experiment
31Phosphate, standard deviation *PO4 std dev±Rouco, Mónica *at the end of the experiment
32Carbon, inorganic, dissolved *DICµmol/kgRouco, Mónica *Coulometric titration *at the end of the experiment
33Carbon, inorganic, dissolved, standard deviation *DIC std dev±Rouco, Mónica *Coulometric titration *at the end of the experiment
34Alkalinity, total *ATµmol/kgRouco, Mónica *Potentiometric titration *at the end of the experiment
35Alkalinity, total, standard deviation *AT std dev±Rouco, Mónica *Potentiometric titration *at the end of the experiment
36pH *pHRouco, Mónica *Calculated using CO2SYS *total scale, at the end of the experiment
37pH, standard deviation *pH std dev±Rouco, Mónica *Calculated using CO2SYS *total scale, at the end of the experiment
38Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmRouco, Mónica *Calculated using CO2SYS *at the end of the experiment
39Partial pressure of carbon dioxide, standard deviation *pCO2 std dev±Rouco, Mónica *Calculated using CO2SYS *at the end of the experiment
40Bicarbonate ion *[HCO3]-µmol/kgRouco, Mónica *Calculated using CO2SYS *at the end of the experiment
41Bicarbonate, standard deviation *[HCO3]- std dev±Rouco, Mónica *Calculated using CO2SYS *at the end of the experiment
42Carbonate ion *[CO3]2-µmol/kgRouco, Mónica *Calculated using CO2SYS *at the end of the experiment
43Carbonate ion, standard deviation *[CO3]2- std dev±Rouco, Mónica *Calculated using CO2SYS *at the end of the experiment
44Carbon dioxide *CO2µmol/kgRouco, Mónica *Calculated using CO2SYS *at the end of the experiment
45Carbon dioxide, standard deviation *CO2 std dev±Rouco, Mónica *Calculated using CO2SYS *at the end of the experiment
46Calcite saturation state *Omega CalRouco, Mónica *Calculated using CO2SYS *at the end of the experiment
47Calcite saturation state, standard deviation *Omega Cal std dev±Rouco, Mónica *Calculated using CO2SYS *at the end of the experiment
48Carbonate system computation flag *CSC flagYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
49pH *pHYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *total scale
50Carbon dioxide *CO2µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
51Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
52Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
53Bicarbonate ion *[HCO3]-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
54Carbonate ion *[CO3]2-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
55Aragonite saturation state *Omega ArgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
56Calcite saturation state *Omega CalYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
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1422 data points

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