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Olischläger, Mark; Wiencke, Christian (2013): Ocean acidification alleviates low-temperature effects on growth and photosynthesis of the red alga Neosiphonia harveyi (Rhodophyta). doi:10.1594/PANGAEA.834202,
Supplement to: Olischläger, M; Wiencke, C (2013): Ocean acidification alleviates low-temperature effects on growth and photosynthesis of the red alga Neosiphonia harveyi (Rhodophyta). Journal of Experimental Botany, 64(18), 5587-5597, doi:10.1093/jxb/ert329

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Abstract:
This study aimed to examine interactive effects between ocean acidification and temperature on the photosynthetic and growth performance of Neosiphonia harveyi. N. harveyi was cultivated at 10 and 17.5 °C at present (~380 µatm), expected future (~800 µatm), and high (~1500 µatm) pCO2. Chlorophyll a fluorescence, net photosynthesis, and growth were measured. The state of the carbon-concentrating mechanism (CCM) was examined by pH-drift experiments (with algae cultivated at 10 °C only) using ethoxyzolamide, an inhibitor of external and internal carbonic anhydrases (exCA and intCA, respectively). Furthermore, the inhibitory effect of acetazolamide (an inhibitor of exCA) and Tris (an inhibitor of the acidification of the diffusive boundary layer) on net photosynthesis was measured at both temperatures. Temperature affected photosynthesis (in terms of photosynthetic efficiency, light saturation point, and net photosynthesis) and growth at present pCO2, but these effects decreased with increasing pCO2. The relevance of the CCM decreased at 10 °C. A pCO2 effect on the CCM could only be shown if intCA and exCA were inhibited. The experiments demonstrate for the first time interactions between ocean acidification and temperature on the performance of a non-calcifying macroalga and show that the effects of low temperature on photosynthesis can be alleviated by increasing pCO2. The findings indicate that the carbon acquisition mediated by exCA and acidification of the diffusive boundary layer decrease at low temperatures but are not affected by the cultivation level of pCO2, whereas the activity of intCA is affected by pCO2. Ecologically, the findings suggest that ocean acidification might affect the biogeographical distribution of N. harveyi.
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-22.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesBeardsley, Christine
2ExperimentExpBeardsley, Christine
3FigureFigBeardsley, Christine
4TreatmentTreatBeardsley, Christine
5IdentificationIDBeardsley, Christine
6Maximal electron transport rate, relativerETR maxµmol e/m2/sBeardsley, Christine
7Light saturationEkµmol/m2/sBeardsley, Christine
8Photosynthetic quantum efficiencyalphaBeardsley, Christine
9Effective quantum yieldYBeardsley, Christine
10IrradianceEµE/m2/sBeardsley, Christine
11Electron transport rateETRµmol e/m2/sBeardsley, Christine
12Net photosynthesis rate, oxygenPN O2µmol/g/hBeardsley, Christine
13pHpHBeardsley, ChristineNBS scale
14Time in hoursTimehBeardsley, Christine
15Inhibition of net photosynthesisInhib NP%Beardsley, Christine
16MassMassgBeardsley, Christinefresh
17Incubation durationInc durdayBeardsley, Christine
18Growth rateµ%/dayBeardsley, Christine14 days of cultivation
19Growth rateµ%/dayBeardsley, Christinebetween day 17 and 24 of cultivation
20Chlorophyll aChl aµg/gBeardsley, Christine
21RatioRatioBeardsley, Christinefresh mass (mg)/Chlorophyll a (mg)
22SalinitySalBeardsley, Christine
23Salinity, standard deviationSal std dev±Beardsley, Christine
24Temperature, waterTemp°CBeardsley, Christineinput
25Temperature, waterTemp°CBeardsley, Christineoutput
26Temperature, water, standard deviationTemp std dev±Beardsley, Christineoutput
27Alkalinity, totalATµmol/kgBeardsley, Christine
28Alkalinity, total, standard deviationAT std dev±Beardsley, Christine
29pHpHBeardsley, ChristinePotentiometrictotal scale, measured at 25
30pH, standard deviationpH std dev±Beardsley, ChristinePotentiometrictotal scale, measured at 25
31Carbon, inorganic, dissolvedDICµmol/kgBeardsley, ChristinePotentiometric titration
32Carbon, inorganic, dissolved, standard deviationDIC std dev±Beardsley, ChristinePotentiometric titration
33Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmBeardsley, ChristineCalculated using CO2SYS
34Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Beardsley, ChristineCalculated using CO2SYS
35Carbon dioxideCO2µmol/kgBeardsley, ChristineCalculated using CO2SYS
36Carbon dioxide, standard deviationCO2 std dev±Beardsley, ChristineCalculated using CO2SYS
37Bicarbonate ion[HCO3]-µmol/kgBeardsley, ChristineCalculated using CO2SYS
38Bicarbonate ion, standard deviation[HCO3]- std dev±Beardsley, ChristineCalculated using CO2SYS
39Carbonate ion[CO3]2-µmol/kgBeardsley, ChristineCalculated using CO2SYS
40Carbonate ion, standard deviation[CO3]2- std dev±Beardsley, ChristineCalculated using CO2SYS
41Calcite saturation stateOmega CalBeardsley, ChristineCalculated using CO2SYS
42Calcite saturation state, standard deviationOmega Cal std dev±Beardsley, ChristineCalculated using CO2SYS
43Aragonite saturation stateOmega ArgBeardsley, ChristineCalculated using CO2SYS
44Aragonite saturation state, standard deviationOmega Arg std dev±Beardsley, ChristineCalculated using CO2SYS
45Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
46pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)
47Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
48Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
50Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
51Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
53Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
54Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
33142 data points

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