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Rokitta, Sebastian D; Rost, Bjoern (2012): Seawater carbonate chemistry and effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi strains RCC 1216 and 1217 during experiments, 2012. PANGAEA,, Supplement to: Rokitta, SD; Rost, B (2012): Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi. Limnology and Oceanography, 57(2), 607-618,

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The effects of ocean acidification on the life-cycle stages of the coccolithophore Emiliania huxleyi and their by light were examined. Calcifying diploid and noncalcifying haploid cells (Roscoff culture collection 1216 and 1217) were acclimated to present-day and elevated CO2 partial pressures (PCO2; 38.5 vs. 101.3 Pa, ., 380 vs. 1000 matm) under low and high light (50 vs. 300 mmol photons m-2 s-1). Growth rates as well as quotas and production rates of C and N were measured. Sources of inorganic C for biomass buildup were using a 14C disequilibrium assay. Photosynthetic O2 evolution was measured as a function of dissolved inorganic C and light by means of membrane-inlet mass spectrometry. The diploid stage responded to elevated PCO2 by shunting resources from the production of particulate inorganic C toward organic C yet keeping the production of total particulate C constant. As the effect of ocean acidification was stronger under low light, the diploid stage might be less affected by increased acidity when energy availability is high. The haploid stage maintained elemental composition and production rates under elevated PCO2. Although both life-cycle stages involve different ways of dealing with elevated PCO2, the responses were generally modulated by energy availability, being typically most pronounced under low light. Additionally, PCO2 responses resembled those induced by high irradiances, indicating that ocean acidification affects the interplay between energy-generating processes (photosynthetic light reactions) and processes competing for energy (biomass buildup and calcification). A conceptual model is put forward explaining why the magnitude of single responses is determined by energy availability.
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesRokitta, Sebastian D
2Experimental treatmentExp treatRokitta, Sebastian D
3SalinitySalRokitta, Sebastian D
4Temperature, waterTemp°CRokitta, Sebastian D
5Nitrate[NO3]-µmol/lRokitta, Sebastian D
6Phosphate[PO4]3-µmol/lRokitta, Sebastian D
7Radiation, photosynthetically activePARµmol/m2/sRokitta, Sebastian D
8Light:Dark cycleL:Dhh:hhRokitta, Sebastian D
9Carbon dioxide, partial pressurepCO2PaRokitta, Sebastian DEstimatedTreatment
10Carbon dioxide, partial pressurepCO2PaRokitta, Sebastian DCalculated using CO2SYSMeasured
11Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Rokitta, Sebastian D
12pHpHRokitta, Sebastian DpH meter, WTW, pH 3000NBS scale
13pH, standard deviationpH std dev±Rokitta, Sebastian D
14Alkalinity, totalATµmol/kgRokitta, Sebastian DAlkalinity, Gran titration (Gran, 1950)
15Alkalinity, total, standard deviationAT std dev±Rokitta, Sebastian D
16Carbon, inorganic, dissolvedDICµmol/kgRokitta, Sebastian DAuto-analyzer, Technicon Traacs 800
17Carbon, inorganic, dissolved, standard deviationDIC std dev±Rokitta, Sebastian D
18Bicarbonate ion[HCO3]-µmol/kgRokitta, Sebastian DCalculated using CO2SYS
19Bicarbonate ion, standard deviation[HCO3]- std dev±Rokitta, Sebastian D
20Carbonate ion[CO3]2-µmol/kgRokitta, Sebastian DCalculated using CO2SYS
21Carbonate ion, standard deviation[CO3]2- std dev±Rokitta, Sebastian D
22Calcite saturation stateOmega CalRokitta, Sebastian DCalculated using CO2SYS
23Calcite saturation state, standard deviationOmega Cal std dev±Rokitta, Sebastian D
24Growth rateµ#/dayRokitta, Sebastian DCalculated, see reference(s)
25Growth rate, standard deviationµ std dev±Rokitta, Sebastian D
26Carbon, inorganic, particulate, per cellPIC/cellpg/#Rokitta, Sebastian DMass spectrometer SL 20-20 (SerCon)
27Particulate inorganic carbon per cell, standard deviationPIC in cell std dev±Rokitta, Sebastian D
28Carbon, organic, particulate, per cellPOCpg/#Rokitta, Sebastian DMass spectrometer SL 20-20 (SerCon)
29Particulate organic carbon content per cell, standard deviationPOC cont std dev±Rokitta, Sebastian D
30Particulate organic nitrogen per cellPON cellpg/#Rokitta, Sebastian DMass spectrometer SL 20-20 (SerCon)
31Particulate organic nitrogen per cell, standard deviationPON cell std dev±Rokitta, Sebastian D
32Particulate inorganic carbon/particulate organic carbon ratioPIC/POCRokitta, Sebastian DCalculated
33Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Rokitta, Sebastian D
34Carbon, organic, particulate/Nitrogen, organic, particulate ratioPOC/PONRokitta, Sebastian DCalculated
35Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviationPOC/PON std dev±Rokitta, Sebastian D
36Production of particulate organic carbon per cellPOC prodpg/#/dayRokitta, Sebastian DCalculated
37Particulate organic carbon, production, standard deviationPOC prod std dev±Rokitta, Sebastian D
38Particulate inorganic carbon production per cellPIC prodpg/#/dayRokitta, Sebastian DCalculated
39Particulate inorganic carbon, production, standard deviationPIC prod std dev±Rokitta, Sebastian D
40Total particulate carbon production per cellTPC prodpg/#/dayRokitta, Sebastian DMass spectrometer SL 20-20 (SerCon)
41Total particulate carbon production, standard deviationTPC prod std dev±Rokitta, Sebastian D
42Production of particulate organic nitrogenPON prodpg/#/dayRokitta, Sebastian DCalculated
43Particulate organic nitrogen production, standard deviationPON prod std dev±Rokitta, Sebastian D
44Chlorophyll a per cellChl apg/#Rokitta, Sebastian DPigments, Turner fluorometer
45Chlorophyll a, standard deviationChl a std dev±Rokitta, Sebastian D
46Chlorophyll a/particulate organic carbon ratioChl a/POCRokitta, Sebastian D
47Chlorophyll a/particulate organic carbon ratio, standard deviationChl a/POC std dev±Rokitta, Sebastian D
48Bicarbonate uptake/net fixation ratio[HCO3]- upt/net fixmol/molRokitta, Sebastian D
49Bicarbonate uptake/net fixation ratio, standard deviation[HCO3]- upt/net fix std dev±Rokitta, Sebastian D
50Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
51pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale; Calculated from means
52Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
53Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
54Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
55Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
56Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
57Carbon, inorganic, dissolvedDICµmol/kgRokitta, Sebastian DCalculated using CO2SYSCalculated from means
58Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
59Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Calculated from means
536 data points

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