Rokitta, Sebastian D; Rost, Björn (2012): Seawater carbonate chemistry and effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi strains RCC 1216 and 1217 during experiments, 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.777432, Supplement to: Rokitta, SD; Rost, B (2012): Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi. Limnology and Oceanography, 57(2), 607-618, https://doi.org/10.4319/lo.2012.57.2.0607
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Abstract:
The effects of ocean acidification on the life-cycle stages of the coccolithophore Emiliania huxleyi and their by light were examined. Calcifying diploid and noncalcifying haploid cells (Roscoff culture collection 1216 and 1217) were acclimated to present-day and elevated CO2 partial pressures (PCO2; 38.5 vs. 101.3 Pa, ., 380 vs. 1000 matm) under low and high light (50 vs. 300 mmol photons m-2 s-1). Growth rates as well as quotas and production rates of C and N were measured. Sources of inorganic C for biomass buildup were using a 14C disequilibrium assay. Photosynthetic O2 evolution was measured as a function of dissolved inorganic C and light by means of membrane-inlet mass spectrometry. The diploid stage responded to elevated PCO2 by shunting resources from the production of particulate inorganic C toward organic C yet keeping the production of total particulate C constant. As the effect of ocean acidification was stronger under low light, the diploid stage might be less affected by increased acidity when energy availability is high. The haploid stage maintained elemental composition and production rates under elevated PCO2. Although both life-cycle stages involve different ways of dealing with elevated PCO2, the responses were generally modulated by energy availability, being typically most pronounced under low light. Additionally, PCO2 responses resembled those induced by high irradiances, indicating that ocean acidification affects the interplay between energy-generating processes (photosynthetic light reactions) and processes competing for energy (biomass buildup and calcification). A conceptual model is put forward explaining why the magnitude of single responses is determined by energy availability.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species
Project(s):
Funding:
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
536 data points
Data
1 Species | 2 Exp treat | 3 Sal | 4 Temp [°C] | 5 [NO3]- [µmol/l] | 6 [PO4]3- [µmol/l] | 7 PAR [µmol/m2/s] | 8 L:D [hh:hh] | 9 pCO2 [Pa] (Treatment, Estimated) | 10 pCO2 [Pa] (Measured, Calculated using CO...) | 11 pCO2 std dev [±] | 12 pH (NBS scale, pH meter, WTW, pH ...) | 13 pH std dev [±] | 14 AT [µmol/kg] (Alkalinity, Gran titration (G...) | 15 AT std dev [±] | 16 DIC [µmol/kg] (Auto-analyzer, Technicon Traa...) | 17 DIC std dev [±] | 18 [HCO3]- [µmol/kg] (Calculated using CO2SYS) | 19 [HCO3]- std dev [±] | 20 [CO3]2- [µmol/kg] (Calculated using CO2SYS) | 21 [CO3]2- std dev [±] | 22 Omega Cal (Calculated using CO2SYS) | 23 Omega Cal std dev [±] | 24 µ [#/day] (Calculated, see reference(s)) | 25 µ std dev [±] | 26 PIC/cell [pg/#] (Mass spectrometer SL 20-20 (S...) | 27 PIC/cell std dev [±] | 28 POC/cell [pg/#] (Mass spectrometer SL 20-20 (S...) | 29 POC cont/cell std dev [±] | 30 PON/cell [pg/#] (Mass spectrometer SL 20-20 (S...) | 31 PON/cell std dev [±] | 32 PIC/POC (Calculated) | 33 PIC/POC ratio std dev [±] | 34 POC/PON (Calculated) | 35 POC/PON std dev [±] | 36 POC prod/cell [pg/#/day] (Calculated) | 37 POC prod std dev [±] | 38 PIC prod/cell [pg/#/day] (Calculated) | 39 PIC prod std dev [±] | 40 TPC prod/cell [pg/#/day] (Mass spectrometer SL 20-20 (S...) | 41 TPC prod std dev [±] | 42 PON prod [pg/#/day] (Calculated) | 43 PON prod std dev [±] | 44 Chl a/cell [pg/#] (Pigments, Turner fluorometer) | 45 Chl a std dev [±] | 46 Chl a/POC | 47 Chl a/POC std dev [±] | 48 [HCO3]- upt/net fix [mol/mol] | 49 [HCO3]- upt/net fix std dev [±] | 50 CSC flag (Calculated using seacarb afte...) | 51 pH (Total scale; Calculated from ...) | 52 CO2 [µmol/kg] (Calculated from means, Calcul...) | 53 pCO2water_SST_wet [µatm] (Calculated from means, Calcul...) | 54 fCO2water_SST_wet [µatm] (Calculated from means, Calcul...) | 55 [HCO3]- [µmol/kg] (Calculated from means, Calcul...) | 56 [CO3]2- [µmol/kg] (Calculated from means, Calcul...) | 57 DIC [µmol/kg] (Calculated from means, Calcul...) | 58 Omega Arg (Calculated from means, Calcul...) | 59 Omega Cal (Calculated from means, Calcul...) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
'Emiliania huxleyi RCC 1216, 2N' | pCO2 low | 32 | 15 | 100 | 6.25 | 50 | 16:08 | 38.5 | 40.4 | 0.4 | 8.112 | 0.014 | 2254 | 20 | 1997 | 44 | 1910 | 17 | 140.1 | 2.1 | 3.47 | 0.05 | 0.63 | 0.14 | 7.45 | 0.54 | 10.35 | 0.63 | 1.25 | 0.08 | 104.00 | 0.16 | 6.97 | 0.31 | 4.72 | 1.13 | 6.56 | 1.55 | 11.29 | 2.59 | 0.79 | 0.19 | 0.16 | 0.01 | 0.02 | 0.002 | 0.85 | 0.01 | 26 | 7.99 | 17.60 | 463.73 | 462.08 | 1931.17 | 131.12 | 2079.90 | 2.03 | 3.19 |
'Emiliania huxleyi RCC 1216, 2N' | pCO2 high | 32 | 15 | 100 | 6.25 | 50 | 16:08 | 101.3 | 97.3 | 11.1 | 7.808 | 0.012 | 2385 | 7 | 2232 | 22 | 2186 | 1 | 80.0 | 2.8 | 1.94 | 0.07 | 0.74 | 0.16 | 11.87 | 1.14 | 2.35 | 0.58 | 2.31 | 0.29 | 0.20 | 0.07 | 5.95 | 0.91 | 8.73 | 2.07 | 1.73 | 0.57 | 10.46 | 2.31 | 1.70 | 0.42 | 0.18 | 0.01 | 0.02 | 0.002 | 0.86 | 0.04 | 26 | 7.69 | 40.07 | 1055.69 | 1051.93 | 2202.41 | 74.91 | 2317.40 | 1.16 | 1.82 |
'Emiliania huxleyi RCC 1216, 2N' | pCO2 low | 32 | 15 | 100 | 6.25 | 300 | 16:08 | 38.5 | 43.5 | 0.2 | 8.083 | 0.040 | 2273 | 5 | 2018 | 15 | 1942 | 4 | 134.6 | 0.6 | 3.33 | 0.01 | 1.02 | 0.15 | 10.81 | 1.41 | 8.78 | 2.34 | 1.52 | 0.37 | 0.82 | 0.22 | 7.48 | 0.26 | 11.03 | 2.19 | 8.96 | 2.74 | 20.00 | 4.32 | 1.55 | 0.44 | 0.12 | 0.03 | 0.01 | 0.003 | 0.89 | 0.03 | 26 | 7.96 | 19.21 | 506.06 | 504.26 | 1966.68 | 124.61 | 2110.50 | 1.93 | 3.03 |
'Emiliania huxleyi RCC 1216, 2N' | pCO2 high | 32 | 15 | 100 | 6.25 | 300 | 16:08 | 101.3 | 110.6 | 14.6 | 7.741 | 0.002 | 2322 | 35 | 2174 | 32 | 2149 | 36 | 68.8 | 1.9 | 1.67 | 0.05 | 0.81 | 0.19 | 15.08 | 2.20 | 9.29 | 2.03 | 2.29 | 0.39 | 0.65 | 0.19 | 7.75 | 0.63 | 12.27 | 3.36 | 7.55 | 2.41 | 20.49 | 5.49 | 1.87 | 0.54 | 0.10 | 0.02 | 0.01 | 0.002 | 0.76 | 0.05 | 26 | 7.62 | 46.36 | 1221.43 | 1217.08 | 2168.28 | 62.76 | 2277.40 | 0.97 | 1.52 |
'Emiliania huxleyi RCC 1217, 1N' | pCO2 low | 32 | 15 | 100 | 6.25 | 50 | 16:08 | 38.5 | 35.6 | 0.4 | 8.176 | 0.005 | 2393 | 24 | 2111 | 19 | 1978 | 21 | 170.8 | 2.8 | 4.23 | 0.07 | 0.87 | 0.12 | 7.70 | 0.67 | 0.58 | 0.12 | 1.49 | 0.15 | 0.07 | 0.01 | 6.00 | 0.13 | 6.73 | 1.11 | 0.51 | 0.12 | 7.24 | 1.21 | 1.30 | 0.23 | 0.19 | 0.01 | 0.02 | 0.002 | 0.87 | 0.01 | 26 | 8.06 | 15.55 | 409.55 | 408.09 | 2003.89 | 159.86 | 2179.30 | 2.48 | 3.88 |
'Emiliania huxleyi RCC 1217, 1N' | pCO2 high | 32 | 15 | 100 | 6.25 | 50 | 16:08 | 101.3 | 35.6 | 1.9 | 7.823 | 0.022 | 2398 | 3 | 2249 | 23 | 2190 | 4 | 83.5 | 1.5 | 2.03 | 0.04 | 0.75 | 0.12 | 10.04 | 2.34 | 0.43 | 0.41 | 2.07 | 0.56 | 0.06 | 0.03 | 5.84 | 0.56 | 7.52 | 2.15 | 0.32 | 0.31 | 7.81 | 2.21 | 1.55 | 0.49 | 0.13 | 0.01 | 0.01 | 0.003 | 0.87 | 0.03 | 26 | 7.70 | 39.31 | 1035.51 | 1031.81 | 2210.65 | 76.95 | 2326.90 | 1.19 | 1.87 |
'Emiliania huxleyi RCC 1217, 1N' | pCO2 low | 32 | 15 | 100 | 6.25 | 300 | 16:08 | 38.5 | 95.7 | 0.4 | 8.177 | 0.019 | 2380 | 10 | 2096 | 24 | 1967 | 18 | 169.5 | 3.4 | 4.20 | 0.08 | 0.18 | 0.20 | 9.65 | 1.90 | 0.61 | 0.35 | 1.63 | 0.35 | 0.06 | 0.04 | 6.92 | 0.33 | 11.40 | 2.94 | 0.73 | 0.43 | 12.12 | 3.11 | 1.93 | 0.53 | 0.09 | 0.02 | 0.01 | 0.003 | 0.89 | 0.03 | 26 | 8.06 | 15.46 | 407.25 | 405.79 | 1992.67 | 158.97 | 2167.10 | 2.47 | 3.86 |
'Emiliania huxleyi RCC 1217, 1N' | pCO2 high | 32 | 15 | 100 | 6.25 | 300 | 16:08 | 101.3 | 35.5 | 5.9 | 7.818 | 0.038 | 2402 | 21 | 2296 | 34 | 2199 | 14 | 81.7 | 7.1 | 1.98 | 0.17 | 0.85 | 0.14 | 10.58 | 1.05 | 0.51 | 0.16 | 1.93 | 0.20 | 0.05 | 0.02 | 6.71 | 0.30 | 8.96 | 1.74 | 0.43 | 0.15 | 9.37 | 1.83 | 1.63 | 0.32 | 0.07 | 0.00 | 0.01 | 0.001 | 0.83 | 0.05 | 26 | 7.70 | 39.36 | 1036.98 | 1033.28 | 2214.34 | 77.09 | 2330.80 | 1.20 | 1.87 |
Reference | pCO2 low | 32 | 15 | 100 | 6.25 | 16:08 | 38.5 | 40.9 | 1.2 | 8.134 | 0.010 | 2411 | 13 | 2134 | 25 | 2030 | 3 | 156.7 | 4.9 | 3.88 | 0.12 | 26 | 8.01 | 17.89 | 471.30 | 469.62 | 2055.63 | 146.18 | 2219.70 | 2.27 | 3.55 | |||||||||||||||||||||||||||
Reference | pCO2 high | 32 | 15 | 100 | 6.25 | 16:08 | 101.3 | 100.9 | 10.4 | 7.770 | 0.025 | 2396 | 17 | 2269 | 45 | 2171 | 4 | 78.2 | 7.7 | 1.90 | 0.19 | 26 | 7.65 | 44.42 | 1170.38 | 1166.21 | 2227.65 | 69.13 | 2341.20 | 1.07 | 1.68 |