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Riebesell, Ulf; Revill, Andrew T; Holdsworth, Daniel G; Volkman, J K (2000): Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.727288, Supplement to: Riebesell, U et al. (2000): The effects of varying CO2 concentration on lipid composition and carbon isotope fractionation in Emiliania huxleyi. Geochimica et Cosmochimica Acta, 64(24), 4179-4192, https://doi.org/10.1016/S0016-7037(00)00474-9

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Abstract:
We have measured the stable carbon isotopic composition of bulk organic matter (POC), alkenones, sterols, fatty acids, and phytol in the coccolithophorid Emiliania huxleyi grown in dilute batch cultures over a wide range of CO2 concentrations (1.1-53.5 micromol L-1). The carbon isotope fractionation of POC (POC) varied by ca. 7 per mil and was positively correlated with aqueous CO2 concentration [CO2aq]. While this result confirms general trends observed for the same alga grown in nitrogen-limited chemostat cultures, considerable differences were obtained in absolute values of POC and in the slope of the relationship of POC with growth rate and [CO2aq]. Also, a significantly greater offset was obtained between the delta13C of alkenones and bulk organic matter in this study compared with previous work (5.4, cf. 3.8 per mil). This suggests that the magnitude of the isotope offset may depend on growth conditions. Relative to POC, individual fatty acids were depleted in 13C by 2.3 per mil to 4.1 per mil, phytol was depleted in 13C by 1.9 per mil, and the major sterol 24-methylcholesta-5,22E-dien-3beta-ol was depleted in 13C by 8.5 per mil. This large spread of delta13C values for different lipid classes in the same alga indicates the need for caution in organic geochemical studies when assigning different sources to lipids that might have delta13C values differing by just a few per mil. Increases in [CO2aq] led to dramatic increases in the alkenone contents per cell and as a proportion of organic carbon, but there was no systematic effect on values of U37k- used for reconstructions of paleo sea surface temperature.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Other studied parameter or process; Pelagos; Phytoplankton; Single species
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Event(s):
Riebesell_etal_2000 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Experimental treatmentExp treatRiebesell, Ulf
2SalinitySalRiebesell, UlfCTD, Sea-Bird SBE 911plus
3Temperature, waterTemp°CRiebesell, UlfCTD, Sea-Bird SBE 911plus
4Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
5pHpHRiebesell, UlfpH meter, WTW, pH 3000NBS scale
6pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
7Carbon, inorganic, dissolvedDICµmol/lRiebesell, UlfCoulometric titration
8Carbon, inorganic, dissolvedDICµmol/kgRiebesell, UlfCalculatedOriginal values in µmol/l, devided by density
9Alkalinity, totalATmmol(eq)/lRiebesell, UlfAlkalinity, Gran titration (Gran, 1950)
10Alkalinity, totalATµmol/kgRiebesell, UlfCalculatedOriginal values in µmol/l, devided by density
11Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
12Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
13Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
14Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
15Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
16Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
17Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
18Growth rateµ#/dayRiebesell, UlfCalculated, see reference(s)Daily
19Growth rateµ#/dayRiebesell, UlfCalculated, see reference(s)Instantaneous
20δ13C, dissolved inorganic carbonδ13C DIC‰ PDBRiebesell, UlfMass spectrometer Finnigan Delta-S
21δ13C, carbon dioxide, aquaticδ13C CO2 aqRiebesell, UlfCalculated, see reference(s)
22δ13C, particulate organic carbonδ13C POC‰ PDBRiebesell, UlfMass spectrometer ANCA-SL 20-20 Europa Scientific
23Isotopic fractionation, during photosynthisεpRiebesell, UlfCalculated after Freeman & Hayes (1992)
24Carbon per cellC/cellpmol/#Riebesell, UlfOriginal values in pg, devided by molar mass
25Nitrogen per cellN/cellpmol/#Riebesell, UlfOriginal values in pg, devided by molar mass
26Nitrogen/Carbon ratioN/CRiebesell, UlfCalculated
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
286 data points

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