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Barcelos e Ramos, Joana; Müller, Marius N; Riebesell, Ulf (2010): Seawater carbonate chemistry and processes during experiments with phytoplankton Emiliania huxleyi (strain Bergen 2005), 2010. doi:10.1594/PANGAEA.736022,
Supplement to: Barcelos e Ramos, J et al. (2010): Short-term response of the coccolithophore Emiliania huxleyi to an abrupt change in seawater carbon dioxide concentrations. Biogeosciences, 7(1), 177-186, doi:10.5194/bg-7-177-2010

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Abstract:
The response of the coccolithophore Emiliania huxleyi to rising CO2 concentrations is well documented for acclimated cultures where cells are exposed to the CO2 treatments for several generations prior to the experiment. The exact number of generations required for acclimation to CO2-induced changes in seawater carbonate chemistry, however, is unknown. Here we show that Emiliania huxleyi's short-term response (26 h) after cultures (grown at 500 µatm) were abruptly exposed to changed CO2 concentrations (~190, 410, 800 and 1500 ?atm) is similar to that obtained with acclimated cultures under comparable conditions in earlier studies. Most importantly, from the lower CO2 levels (190 and 410 ?atm) to 750 and 1500 µatm calcification decreased and organic carbon fixation increased within the first 8 to 14 h after exposing the cultures to changes in carbonate chemistry. This suggests that Emiliania huxleyi rapidly alters the rates of essential metabolical processes in response to changes in seawater carbonate chemistry, establishing a new physiological "state" (acclimation) within a matter of hours. If this relatively rapid response applies to other phytoplankton species, it may simplify interpretation of studies with natural communities (e.g. mesocosm studies and ship-board incubations), where often it is not feasible to allow for a pre-conditioning phase before starting experimental incubations.
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1DescriptionDescriptionBarcelos e Ramos, JoanaEnd of experiment
2Experimental treatmentExp trtmBarcelos e Ramos, Joana
3SalinitySalBarcelos e Ramos, Joana
4Temperature, waterTemp°CBarcelos e Ramos, Joana
5Radiation, photosynthetically activePARµE/m2/sBarcelos e Ramos, Joana
6Light:Dark cycleL:Dhh:hhBarcelos e Ramos, Joana
7PhosphatePO4µmol/lBarcelos e Ramos, JoanaDetermination of phosphate (Murphy & Riley, 1962)
8Alkalinity, totalATµmol/kgBarcelos e Ramos, JoanaMetrohm Titrando titrator
9Carbon, inorganic, dissolvedDICµmol/kgBarcelos e Ramos, JoanaAutomated segmented-flow analyzer (Quaatro)
10Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmBarcelos e Ramos, JoanaCalculated using CO2SYSConstants Roy et al. 1993
11pHpHBarcelos e Ramos, JoanaCalculated using CO2SYSFree scale, Constants Roy et al. 1993
12pHpHBarcelos e Ramos, JoanaCalculated using CO2SYSTotal scale, Constants Roy et al. 1993
13Bicarbonate ion[HCO3]-µmol/kgBarcelos e Ramos, JoanaCalculated using CO2SYSConstants Roy et al. 1993
14Carbonate ion[CO3]2-µmol/kgBarcelos e Ramos, JoanaCalculated using CO2SYSConstants Roy et al. 1993
15Carbon dioxideCO2µmol/kgBarcelos e Ramos, JoanaCalculated using CO2SYSConstants Roy et al. 1993
16Calcite saturation stateOmega CalBarcelos e Ramos, JoanaCalculated using CO2SYSConstants Roy et al. 1993
17Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
18pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
19Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
20Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
21Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
22Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
23Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
24Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
25Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
26Organic carbon fixation per cell per hourOrg C fixpg/#/hBarcelos e Ramos, Joanasee reference(s)Replicate 1
27Organic carbon fixation per cell per hourOrg C fixpg/#/hBarcelos e Ramos, Joanasee reference(s)Replicate 2
28Calcification rate of carbon per cellCalc rate Cpg/#/hBarcelos e Ramos, Joanasee reference(s)Replicate 1
29Calcification rate of carbon per cellCalc rate Cpg/#/hBarcelos e Ramos, Joanasee reference(s)Replicate 2
30Total carbon fixation per cell per hourTotal C fixpg/#/hBarcelos e Ramos, Joanasee reference(s)Replicate 1
31Total carbon fixation per cell per hourTotal C fixpg/#/hBarcelos e Ramos, Joanasee reference(s)Replicate 2
32Cumulative carbon fixation per cellCum C fixpg/#Barcelos e Ramos, Joanasee reference(s)Replicate 1
33Cumulative carbon fixation per cellCum C fixpg/#Barcelos e Ramos, Joanasee reference(s)Replicate 2
34Particulate inorganic carbon/particulate organic carbon ratioPIC/POC ratioBarcelos e Ramos, Joanasee reference(s)Replicate 1
35Particulate inorganic carbon/particulate organic carbon ratioPIC/POC ratioBarcelos e Ramos, Joanasee reference(s)Replicate 2
36Emiliania huxleyi, diameterE. huxleyi diamµmBarcelos e Ramos, JoanaCoulter Counter Z series (Beckman Coulter)Replicate 1
37Emiliania huxleyi, diameterE. huxleyi diamµmBarcelos e Ramos, JoanaCoulter Counter Z series (Beckman Coulter)Replicate 2
38Emiliania huxleyi, diameterE. huxleyi diamµmBarcelos e Ramos, JoanaCoulter Counter Z series (Beckman Coulter)Replicate 3
39Maximum photochemical quantum yield of photosystem IIFv/FmBarcelos e Ramos, JoanaPAM (PhytoPAM, Phyto-ED Walz, PPAA0138)
40Emiliania huxleyiE. huxleyi#/lBarcelos e Ramos, JoanaCoulter Counter Z series (Beckman Coulter)Replicate 1
41Emiliania huxleyiE. huxleyi#/lBarcelos e Ramos, JoanaCoulter Counter Z series (Beckman Coulter)Replicate 2
42Emiliania huxleyiE. huxleyi#/lBarcelos e Ramos, JoanaCoulter Counter Z series (Beckman Coulter)Replicate 3
Size:
834 data points

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