Data Description

  RIS BibTeX
Citation:
Borchard, C et al. (2012): Seawater carbonate chemistry and biological processes of Emiliania huxleyi (PML B92/11) during experiments, 2011. doi:10.1594/PANGAEA.774802,
Supplement to: Borchard, Corinna; Borges, Alberto Vieira; Händel, Nicole; Engel, Anja (2011): Biogeochemical response of Emiliania huxleyi (PML B92/11) to elevated CO2 and temperature under phosphorous limitation: A chemostat study. Journal of Experimental Marine Biology and Ecology, 410, 61-71, doi:10.1016/j.jembe.2011.10.004
Abstract:
The present study investigates the combined effect of phosphorous limitation, elevated partial pressure of CO2 (pCO2) and temperature on a calcifying strain of Emiliania huxleyi (PML B92/11) by means of a fully controlled continuous culture facility. Two levels of phosphorous limitation were consecutively applied by renewal of culture media (N:P = 26) at dilution rates (D) of 0.3 d- and 0.1 d-1. CO2 and temperature conditions were 300, 550 and 900 µatm pCO2 at 14 °C and 900 µatm pCO2 at 18 °C. In general, the steady state cell density and particulate organic carbon (POC) production increased with pCO2, yielding significantly higher concentrations in cultures grown at 900 µatm pCO2 compared to 300 and 550 µatm pCO2. At 900 µatm pCO2, elevation of temperature as expected for a greenhouse ocean, further increased cell densities and POC concentrations. In contrast to POC concentration, C-quotas (pmol C cell-1) were similar at D = 0.3 d-1 in all cultures. At D = 0.1 d-1, a reduction of C-quotas by up to 15% was observed in the 900 µatm pCO2 at 18 °C culture. As a result of growth rate reduction, POC:PON:POP ratios deviated strongly from the Redfield ratio, primarily due to an increase in POC. Ratios of particulate inorganic and organic carbon (PIC:POC) ranged from 0.14 to 0.18 at D = 0.3 d-1, and from 0.11 to 0.17 at D = 0.1 d-1, with variations primarily induced by the changes in POC. At D = 0.1 d-1, cell volume was reduced by up to 22% in cultures grown at 900 µatm pCO2. Our results indicate that changes in pCO2, temperature and phosphorus supply affect cell density, POC concentration and size of E. huxleyi (PML B92/11) to varying degrees, and will likely impact bloom development as well as biogeochemical cycling in a greenhouse ocean.
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatment *Exp trtmEngel, Anja *
2Sample ID *Sample IDEngel, Anja *
3Salinity *SalEngel, Anja *Salinometer - Tropic Marin Sea Salt, Dr. Biener GmbH, Germany *
4Temperature, water *Temp°CEngel, Anja *WTW 340i pH-analyzer and WTW SenTix 81-electrode *
5Nitrate *NO3µmol/lEngel, Anja *Spectrophotometry *
6Phosphate *PO4µmol/lEngel, Anja *Spectrophotometry *
7Light:Dark cycle *L:Dhh:hhEngel, Anja *
8Radiation, photosynthetically active *PARµE/m2/sEngel, Anja *
9Alkalinity, total *ATµmol/kgEngel, Anja *Measured *
10pH *pHEngel, Anja *WTW 340i pH-analyzer and WTW SenTix 81-electrode *NBS scale
11Carbon, inorganic, dissolved *DICµmol/kgEngel, Anja *Calculated using CO2SYS *
12Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmEngel, Anja *Infrared gas analyzer (LI-COR LI-6252) *
13Carbon dioxide *CO2µmol/kgEngel, Anja *Calculated using CO2SYS *
14Bicarbonate ion *[HCO3]-µmol/kgEngel, Anja *Calculated using CO2SYS *
15Carbonate ion *[CO3]2-µmol/kgEngel, Anja *Calculated using CO2SYS *
16Calcite saturation state *Omega CalEngel, Anja *Calculated using CO2SYS *
17Revelle factor *REngel, Anja *
18Experiment day *Exp daydayEngel, Anja *
19Nitrogen, inorganic, dissolved *DINµmol/lEngel, Anja *Element analyser CNS, EURO EA *
20Nitrogen, inorganic, dissolved *DINµmol/lEngel, Anja *Element analyser CNS, EURO EA *
21Nitrogen, organic *N orgµmol/lEngel, Anja *Element analyser CNS, EURO EA *
22Particulate organic nitrogen production *PON prodµmol/l/dayEngel, Anja *Calculated, see reference(s) *
23Particulate organic nitrogen per cell *PON cellpmol/#Engel, Anja *Calculated, see reference(s) *
24Production of particulate organic nitrogen *PON prodpg/#/dayEngel, Anja *Calculated, see reference(s) *
25Phosphorus, inorganic *P inorgµmol/lEngel, Anja *Colorimetry *
26Phosphorus, inorganic *P inorgµmol/lEngel, Anja *Colorimetry *
27Phosphorus, organic, particulate *POPµmol/lEngel, Anja *Colorimetry *
28Particulate organic phosphorus production *POP prodµmol/l/dayEngel, Anja *Calculated, see reference(s) *
29Particulate organic phosphorus per cell *POP cellpmol/#Engel, Anja *Calculated, see reference(s) *
30Production of particulate organic phosphorus, per cell *POP prodpmol/#/dayEngel, Anja *Calculated, see reference(s) *
31Carbon, organic, particulate *POCµmol/lEngel, Anja *Element analyser CNS, EURO EA *
32Particulate organic carbon production *POC prodµmol/l/dayEngel, Anja *Calculated, see reference(s) *
33Particulate organic carbon content per cell *POC contpmol/#Engel, Anja *Element analyser CNS, EURO EA *
34Particulate organic carbon production per cell *POC prod cellµmol/#/dayEngel, Anja *Calculated, see reference(s) *
35Particulate organic carbon/particulate organic nitrogen ratio *POC/PONEngel, Anja *Calculated, see reference(s) *
36Particulate organic carbon/particulate organic phosphorus ratio *POC/POPEngel, Anja *Calculated, see reference(s) *
37Particulate organic nitrogen/particulate organic phosphorus ratio *PON/POPEngel, Anja *Calculated, see reference(s) *
38Carbonate system computation flag *CSC flagNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
39pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Total scale
40Carbon dioxide *CO2µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
41Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
42Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
43Bicarbonate ion *[HCO3]-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
44Carbonate ion *[CO3]2-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
45Carbon, inorganic, dissolved *DICµmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
46Alkalinity, total *ATµmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
47Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
48Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
License:
Size:
1068 data points

Download Data

Download dataset as tab-delimited text (use the following character encoding: )

View dataset as HTML