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Borchard, Corinna; Borges, Alberto Vieira; Händel, Nicole; Engel, Anja (2012): Seawater carbonate chemistry and biological processes of Emiliania huxleyi (PML B92/11) during experiments, 2011. doi:10.1594/PANGAEA.774802,
Supplement to: Borchard, C et al. (2011): Biogeochemical response of Emiliania huxleyi (PML B92/11) to elevated CO2 and temperature under phosphorous limitation: A chemostat study. Journal of Experimental Marine Biology and Ecology, 410, 61-71, doi:10.1016/j.jembe.2011.10.004

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Abstract:
The present study investigates the combined effect of phosphorous limitation, elevated partial pressure of CO2 (pCO2) and temperature on a calcifying strain of Emiliania huxleyi (PML B92/11) by means of a fully controlled continuous culture facility. Two levels of phosphorous limitation were consecutively applied by renewal of culture media (N:P = 26) at dilution rates (D) of 0.3 d- and 0.1 d-1. CO2 and temperature conditions were 300, 550 and 900 µatm pCO2 at 14 °C and 900 µatm pCO2 at 18 °C. In general, the steady state cell density and particulate organic carbon (POC) production increased with pCO2, yielding significantly higher concentrations in cultures grown at 900 µatm pCO2 compared to 300 and 550 µatm pCO2. At 900 µatm pCO2, elevation of temperature as expected for a greenhouse ocean, further increased cell densities and POC concentrations. In contrast to POC concentration, C-quotas (pmol C cell-1) were similar at D = 0.3 d-1 in all cultures. At D = 0.1 d-1, a reduction of C-quotas by up to 15% was observed in the 900 µatm pCO2 at 18 °C culture. As a result of growth rate reduction, POC:PON:POP ratios deviated strongly from the Redfield ratio, primarily due to an increase in POC. Ratios of particulate inorganic and organic carbon (PIC:POC) ranged from 0.14 to 0.18 at D = 0.3 d-1, and from 0.11 to 0.17 at D = 0.1 d-1, with variations primarily induced by the changes in POC. At D = 0.1 d-1, cell volume was reduced by up to 22% in cultures grown at 900 µatm pCO2. Our results indicate that changes in pCO2, temperature and phosphorus supply affect cell density, POC concentration and size of E. huxleyi (PML B92/11) to varying degrees, and will likely impact bloom development as well as biogeochemical cycling in a greenhouse ocean.
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatmentExp trtmEngel, Anja
2Sample IDSample IDEngel, Anja
3SalinitySalEngel, AnjaSalinometer - Tropic Marin Sea Salt, Dr. Biener GmbH, Germany
4Temperature, waterTemp°CEngel, AnjaWTW 340i pH-analyzer and WTW SenTix 81-electrode
5NitrateNO3µmol/lEngel, AnjaSpectrophotometry
6PhosphatePO4µmol/lEngel, AnjaSpectrophotometry
7Light:Dark cycleL:Dhh:hhEngel, Anja
8Radiation, photosynthetically activePARµE/m2/sEngel, Anja
9Alkalinity, totalATµmol/kgEngel, AnjaMeasured
10pHpHEngel, AnjaWTW 340i pH-analyzer and WTW SenTix 81-electrodeNBS scale
11Carbon, inorganic, dissolvedDICµmol/kgEngel, AnjaCalculated using CO2SYS
12Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmEngel, AnjaInfrared gas analyzer (LI-COR LI-6252)
13Carbon dioxideCO2µmol/kgEngel, AnjaCalculated using CO2SYS
14Bicarbonate ion[HCO3]-µmol/kgEngel, AnjaCalculated using CO2SYS
15Carbonate ion[CO3]2-µmol/kgEngel, AnjaCalculated using CO2SYS
16Calcite saturation stateOmega CalEngel, AnjaCalculated using CO2SYS
17Revelle factorREngel, Anja
18Experiment dayExp daydayEngel, Anja
19Nitrogen, inorganic, dissolvedDINµmol/lEngel, AnjaElement analyser CNS, EURO EA
20Nitrogen, inorganic, dissolvedDINµmol/lEngel, AnjaElement analyser CNS, EURO EA
21Nitrogen, organicN orgµmol/lEngel, AnjaElement analyser CNS, EURO EA
22Particulate organic nitrogen productionPON prodµmol/l/dayEngel, AnjaCalculated, see reference(s)
23Particulate organic nitrogen per cellPON cellpmol/#Engel, AnjaCalculated, see reference(s)
24Production of particulate organic nitrogenPON prodpg/#/dayEngel, AnjaCalculated, see reference(s)
25Phosphorus, inorganicP inorgµmol/lEngel, AnjaColorimetry
26Phosphorus, inorganicP inorgµmol/lEngel, AnjaColorimetry
27Phosphorus, organic, particulatePOPµmol/lEngel, AnjaColorimetry
28Particulate organic phosphorus productionPOP prodµmol/l/dayEngel, AnjaCalculated, see reference(s)
29Particulate organic phosphorus per cellPOP cellpmol/#Engel, AnjaCalculated, see reference(s)
30Production of particulate organic phosphorus, per cellPOP prodpmol/#/dayEngel, AnjaCalculated, see reference(s)
31Carbon, organic, particulatePOCµmol/lEngel, AnjaElement analyser CNS, EURO EA
32Particulate organic carbon productionPOC prodµmol/l/dayEngel, AnjaCalculated, see reference(s)
33Particulate organic carbon content per cellPOC contpmol/#Engel, AnjaElement analyser CNS, EURO EA
34Particulate organic carbon production per cellPOC prod cellµmol/#/dayEngel, AnjaCalculated, see reference(s)
35Particulate organic carbon/particulate organic nitrogen ratioPOC/PONEngel, AnjaCalculated, see reference(s)
36Particulate organic carbon/particulate organic phosphorus ratioPOC/POPEngel, AnjaCalculated, see reference(s)
37Particulate organic nitrogen/particulate organic phosphorus ratioPON/POPEngel, AnjaCalculated, see reference(s)
38Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
39pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
40Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
41Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
42Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
43Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
44Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
45Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
46Alkalinity, totalATµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
47Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
48Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
Size:
1068 data points

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