Borchard, Corinna; Borges, Alberto Vieira; Händel, Nicole; Engel, Anja (2011): Seawater carbonate chemistry and biological processes of Emiliania huxleyi (PML B92/11) during experiments, 2011 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.774802, Supplement to: Borchard, C et al. (2011): Biogeochemical response of Emiliania huxleyi (PML B92/11) to elevated CO2 and temperature under phosphorous limitation: A chemostat study. Journal of Experimental Marine Biology and Ecology, 410, 61-71, https://doi.org/10.1016/j.jembe.2011.10.004
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Abstract:
The present study investigates the combined effect of phosphorous limitation, elevated partial pressure of CO2 (pCO2) and temperature on a calcifying strain of Emiliania huxleyi (PML B92/11) by means of a fully controlled continuous culture facility. Two levels of phosphorous limitation were consecutively applied by renewal of culture media (N:P = 26) at dilution rates (D) of 0.3 d- and 0.1 d-1. CO2 and temperature conditions were 300, 550 and 900 µatm pCO2 at 14 °C and 900 µatm pCO2 at 18 °C. In general, the steady state cell density and particulate organic carbon (POC) production increased with pCO2, yielding significantly higher concentrations in cultures grown at 900 µatm pCO2 compared to 300 and 550 µatm pCO2. At 900 µatm pCO2, elevation of temperature as expected for a greenhouse ocean, further increased cell densities and POC concentrations. In contrast to POC concentration, C-quotas (pmol C cell-1) were similar at D = 0.3 d-1 in all cultures. At D = 0.1 d-1, a reduction of C-quotas by up to 15% was observed in the 900 µatm pCO2 at 18 °C culture. As a result of growth rate reduction, POC:PON:POP ratios deviated strongly from the Redfield ratio, primarily due to an increase in POC. Ratios of particulate inorganic and organic carbon (PIC:POC) ranged from 0.14 to 0.18 at D = 0.3 d-1, and from 0.11 to 0.17 at D = 0.1 d-1, with variations primarily induced by the changes in POC. At D = 0.1 d-1, cell volume was reduced by up to 22% in cultures grown at 900 µatm pCO2. Our results indicate that changes in pCO2, temperature and phosphorus supply affect cell density, POC concentration and size of E. huxleyi (PML B92/11) to varying degrees, and will likely impact bloom development as well as biogeochemical cycling in a greenhouse ocean.
Keyword(s):
Project(s):
Funding:
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1068 data points
Data
1 Exp treat | 2 Sample ID | 3 Sal (Salinometer - Tropic Marin Se...) | 4 Temp [°C] (WTW 340i pH-analyzer and WTW ...) | 5 [NO3]- [µmol/l] (Spectrophotometry) | 6 [PO4]3- [µmol/l] (Spectrophotometry) | 7 L:D [hh:hh] | 8 PAR [µmol/m2/s] | 9 AT [µmol/kg] (Measured) | 10 pH (NBS scale, WTW 340i pH-analyz...) | 11 DIC [µmol/kg] (Calculated using CO2SYS) | 12 pCO2water_SST_wet [µatm] (Infrared gas analyzer (LI-COR...) | 13 CO2 [µmol/kg] (Calculated using CO2SYS) | 14 [HCO3]- [µmol/kg] (Calculated using CO2SYS) | 15 [CO3]2- [µmol/kg] (Calculated using CO2SYS) | 16 Omega Cal (Calculated using CO2SYS) | 17 R | 18 Exp day [day] | 19 DIN [µmol/l] (Element analyser CNS, EURO EA) | 20 DIN [µmol/l] (Element analyser CNS, EURO EA) | 21 N org [µmol/l] (Element analyser CNS, EURO EA) | 22 PON prod [µmol/l/day] (Calculated, see reference(s)) | 23 PON/cell [pmol/#] (Calculated, see reference(s)) | 24 PON prod [pg/#/day] (Calculated, see reference(s)) | 25 P inorg [µmol/l] (Colorimetry) | 26 P inorg [µmol/l] (Colorimetry) | 27 POP [µmol/l] (Colorimetry) | 28 POP prod [µmol/l/day] (Calculated, see reference(s)) | 29 POP/cell [pmol/#] (Calculated, see reference(s)) | 30 POP prod/cell [pmol/#/day] (Calculated, see reference(s)) | 31 POC [µmol/l] (Element analyser CNS, EURO EA) | 32 POC prod [µmol/l/day] (Calculated, see reference(s)) | 33 POC/cell [pmol/#] (Element analyser CNS, EURO EA) | 34 POC prod/cell [µmol/#/day] (Calculated, see reference(s)) | 35 POC/PON (Calculated, see reference(s)) | 36 POC/POP (Calculated, see reference(s)) | 37 PON/POP (Calculated, see reference(s)) | 38 CSC flag (Calculated using seacarb afte...) | 39 pH (Total scale, Calculated using...) | 40 CO2 [µmol/kg] (Calculated using seacarb afte...) | 41 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 42 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 43 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 44 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 45 DIC [µmol/kg] (Calculated using seacarb afte...) | 46 AT [µmol/kg] (Calculated using seacarb afte...) | 47 Omega Arg (Calculated using seacarb afte...) | 48 Omega Cal (Calculated using seacarb afte...) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
300/14 | equi | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 2281.0 | 8.3 | 2028.6 | 287.4 | 11.3 | 1835.8 | 181.5 | 4.4 | 11 | 29 | 8.18 | 11.26 | 286.98 | 285.94 | 1836.16 | 181.17 | 2028.60 | 2280.60 | 2.81 | 4.41 | ||||||||||||||||||||
550/14 | equi | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 2281.0 | 8.1 | 2136.3 | 542.9 | 21.3 | 2000.9 | 114.1 | 2.8 | 14 | 29 | 7.94 | 21.27 | 541.99 | 540.03 | 2001.09 | 113.94 | 2136.30 | 2280.80 | 1.77 | 2.77 | ||||||||||||||||||||
900/14 | equi | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 2281.0 | 7.9 | 2203.8 | 881.9 | 34.6 | 2092.4 | 76.8 | 1.9 | 17 | 29 | 7.75 | 34.56 | 880.51 | 877.33 | 2092.55 | 76.69 | 2203.80 | 2280.90 | 1.19 | 1.87 | ||||||||||||||||||||
900/18 | equi | 31.5 | 18 | 29 | 1.1 | 16:08 | 300 | 2281.0 | 7.9 | 2206.1 | 897.5 | 35.2 | 2095.1 | 75.7 | 1.8 | 17 | 29 | 7.73 | 31.19 | 895.57 | 892.50 | 2001.41 | 82.60 | 2115.20 | 2204.50 | 1.30 | 2.02 | ||||||||||||||||||||
300/14 | 2 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1459.1 | 8.2 | 1299.9 | 225.9 | 8.9 | 1193.5 | 97.6 | 2.4 | 10 | 14 | 29.61 | 0.35 | 20.7 | 6.3 | 0.0722 | 0.308 | 1.13 | 0.01 | 0.829 | 0.252 | 2897.39 | 881.814 | 499.69 | 152.08 | 1.75 | 0.53 | 24.2 | 602.81 | 24.9 | 29 | 8.09 | 8.85 | 225.60 | 224.79 | 1193.65 | 97.39 | 1299.90 | 1458.80 | 1.51 | 2.37 |
300/14 | 3 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1420.1 | 8.1 | 1283.3 | 259.3 | 10.2 | 1188.8 | 84.3 | 2.1 | 11 | 17 | 29.61 | 0.56 | 21.2 | 6.4 | 0.0714 | 0.304 | 1.13 | 0.06 | 0.854 | 0.260 | 2880.86 | 876.782 | 571.48 | 173.93 | 1.93 | 0.59 | 27.0 | 668.82 | 24.8 | 29 | 8.03 | 10.16 | 258.99 | 258.06 | 1188.96 | 84.17 | 1283.30 | 1419.80 | 1.30 | 2.05 |
300/14 | 4 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1053.8 | 8.0 | 980.6 | 311.6 | 12.2 | 925.8 | 42.6 | 1.0 | 12 | 22 | 29.61 | 0.25 | 16.9 | 1.7 | 0.0586 | 0.080 | 1.13 | 0.00 | 0.836 | 0.082 | 2900.98 | 283.791 | 706.30 | 69.09 | 2.45 | 0.24 | 41.8 | 844.51 | 20.2 | 29 | 7.84 | 12.22 | 311.24 | 310.11 | 925.91 | 42.48 | 980.60 | 1053.70 | 0.66 | 1.03 |
300/14 | 5 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1020.3 | 8.0 | 936.7 | 261.3 | 10.3 | 880.5 | 45.9 | 1.1 | 11 | 25 | 29.61 | 0.66 | 21.8 | 2.1 | 0.0697 | 0.095 | 1.13 | 0.00 | 1.038 | 0.102 | 3326.21 | 325.390 | 873.04 | 85.41 | 2.80 | 0.27 | 40.1 | 840.72 | 21.0 | 29 | 7.90 | 10.24 | 260.94 | 260.00 | 880.63 | 45.83 | 936.70 | 1020.20 | 0.71 | 1.12 |
300/14 | 6 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1019.7 | 8.0 | 917.6 | 247.9 | 9.7 | 861.5 | 46.3 | 1.1 | 11 | 28 | 29.61 | 0.36 | 22.7 | 2.2 | 0.0688 | 0.094 | 1.13 | 1.030 | 0.101 | 3115.24 | 304.752 | 934.37 | 91.41 | 2.83 | 0.28 | 41.1 | 906.97 | 22.1 | 29 | 7.91 | 9.73 | 247.98 | 247.08 | 861.69 | 46.18 | 917.60 | 1003.20 | 0.72 | 1.12 | |
550/14 | 2 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1423.9 | 7.9 | 1357.7 | 515.5 | 20.2 | 1287.7 | 49.8 | 1.2 | 15 | 14 | 28.16 | 0.70 | 24.6 | 7.5 | 0.0715 | 0.305 | 1.11 | 0.00 | 1.008 | 0.307 | 2929.84 | 891.692 | 637.31 | 193.97 | 1.85 | 0.56 | 25.9 | 632.52 | 24.4 | 29 | 7.77 | 20.23 | 515.40 | 513.54 | 1287.85 | 49.63 | 1357.70 | 1423.70 | 0.77 | 1.21 |
550/14 | 3 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1445.6 | 7.9 | 1379.7 | 528.1 | 20.7 | 1308.8 | 50.2 | 1.2 | 15 | 17 | 28.16 | 0.41 | 23.5 | 7.2 | 0.0673 | 0.287 | 1.11 | 0.03 | 0.878 | 0.267 | 2514.55 | 765.299 | 616.03 | 187.49 | 1.76 | 0.54 | 26.2 | 701.36 | 26.8 | 29 | 7.76 | 20.71 | 527.78 | 525.88 | 1308.93 | 50.06 | 1379.70 | 1445.40 | 0.78 | 1.22 |
550/14 | 4 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1045.4 | 7.7 | 1013.7 | 545.4 | 21.4 | 965.8 | 26.5 | 0.6 | 15 | 22 | 28.16 | 0.80 | 19.3 | 1.9 | 0.0632 | 0.087 | 1.11 | 0.00 | 0.807 | 0.079 | 2644.11 | 258.663 | 783.76 | 76.67 | 2.57 | 0.25 | 40.7 | 971.54 | 23.9 | 29 | 7.62 | 21.41 | 545.53 | 543.56 | 965.92 | 26.37 | 1013.70 | 1045.30 | 0.41 | 0.64 |
550/14 | 5 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1095.1 | 7.8 | 1059.0 | 539.1 | 21.2 | 1008.6 | 29.2 | 0.7 | 15 | 25 | 28.16 | 0.41 | 19.7 | 1.9 | 0.0689 | 0.094 | 1.11 | 0.00 | 0.887 | 0.087 | 3098.80 | 303.143 | 772.10 | 75.53 | 2.70 | 0.26 | 39.2 | 870.89 | 22.2 | 29 | 7.64 | 21.16 | 539.06 | 537.11 | 1008.73 | 29.11 | 1059.00 | 1095.00 | 0.45 | 0.71 |
550/14 | 6 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1145.9 | 7.8 | 1099.3 | 552.8 | 21.7 | 1046.9 | 30.7 | 0.7 | 15 | 28 | 28.16 | 0.47 | 21.5 | 2.1 | 0.0778 | 0.107 | 1.11 | 0.790 | 0.077 | 2858.64 | 279.649 | 779.67 | 76.27 | 2.82 | 0.28 | 36.3 | 987.13 | 27.2 | 29 | 7.65 | 21.68 | 552.45 | 550.45 | 1047.02 | 30.60 | 1099.30 | 1136.60 | 0.47 | 0.75 | |
900/14 | 2 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1522.2 | 7.7 | 1492.5 | 842.2 | 33.1 | 1422.3 | 37.2 | 0.9 | 17 | 14 | 29.54 | 0.94 | 24.6 | 7.5 | 0.0712 | 0.303 | 1.12 | 0.00 | 0.875 | 0.266 | 2527.10 | 769.118 | 565.03 | 171.97 | 1.63 | 0.50 | 22.9 | 646.03 | 28.2 | 29 | 7.60 | 33.01 | 841.09 | 838.05 | 1422.39 | 37.10 | 1492.50 | 1522.10 | 0.57 | 0.90 |
900/14 | 3 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1512.4 | 7.6 | 1498.8 | 1005.2 | 39.4 | 1427.9 | 31.4 | 0.8 | 17 | 17 | 29.54 | 0.80 | 25.9 | 7.9 | 0.0689 | 0.294 | 1.12 | 0.02 | 0.877 | 0.267 | 2337.68 | 711.469 | 649.47 | 197.66 | 1.73 | 0.53 | 25.1 | 740.47 | 29.5 | 29 | 7.52 | 39.44 | 1005.01 | 1001.38 | 1428.06 | 31.29 | 1498.80 | 1512.30 | 0.48 | 0.76 |
900/14 | 4 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1236.8 | 7.5 | 1240.6 | 1055.1 | 41.4 | 1178.8 | 20.4 | 0.5 | 16 | 22 | 29.54 | 1.08 | 22.0 | 2.1 | 0.0731 | 0.100 | 1.12 | 0.00 | 0.852 | 0.083 | 2835.25 | 277.361 | 855.57 | 83.70 | 2.85 | 0.28 | 39.0 | 1004.20 | 25.8 | 29 | 7.42 | 41.36 | 1053.94 | 1050.14 | 1178.90 | 20.34 | 1240.60 | 1236.80 | 0.32 | 0.50 |
900/14 | 5 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1269.2 | 7.5 | 1272.0 | 1062.2 | 41.7 | 1209.0 | 21.3 | 0.5 | 16 | 25 | 29.54 | 0.37 | 23.2 | 2.3 | 0.0627 | 0.086 | 1.12 | 0.00 | 0.824 | 0.081 | 2222.32 | 217.401 | 819.68 | 80.19 | 2.21 | 0.22 | 35.3 | 995.25 | 28.2 | 29 | 7.43 | 41.67 | 1061.62 | 1057.79 | 1209.10 | 21.24 | 1272.00 | 1269.10 | 0.33 | 0.52 |
900/14 | 6 | 31.5 | 14 | 29 | 1.1 | 16:08 | 300 | 1274.7 | 7.5 | 1247.0 | 1050.8 | 41.2 | 1185.1 | 20.7 | 0.5 | 16 | 28 | 29.54 | 0.30 | 27.1 | 2.6 | 0.0751 | 0.103 | 1.12 | 0.910 | 0.089 | 2522.50 | 246.767 | 900.96 | 88.14 | 2.50 | 0.24 | 33.3 | 990.48 | 29.8 | 29 | 7.42 | 41.21 | 1049.98 | 1046.19 | 1185.16 | 20.63 | 1247.00 | 1243.80 | 0.32 | 0.50 | |
900/18 | 2 | 31.5 | 18 | 29 | 1.1 | 16:08 | 300 | 1292.8 | 7.7 | 1262.5 | 815.2 | 28.4 | 1201.3 | 32.8 | 0.8 | 16 | 14 | 29.24 | 0.62 | 25.0 | 7.6 | 0.0599 | 0.255 | 1.13 | 0.00 | 1.180 | 0.359 | 2829.44 | 861.133 | 739.80 | 225.16 | 1.77 | 0.54 | 29.6 | 626.71 | 21.2 | 29 | 7.55 | 28.36 | 814.20 | 811.41 | 1201.40 | 32.74 | 1262.50 | 1292.70 | 0.51 | 0.80 |
900/18 | 3 | 31.5 | 18 | 29 | 1.1 | 16:08 | 300 | 1325.3 | 7.6 | 1308.3 | 988.3 | 34.4 | 1244.9 | 29.1 | 0.7 | 16 | 17 | 29.24 | 0.32 | 26.5 | 8.1 | 0.0605 | 0.258 | 1.13 | 0.02 | 0.942 | 0.287 | 2152.46 | 655.096 | 777.62 | 236.67 | 1.78 | 0.54 | 29.4 | 825.38 | 28.1 | 29 | 7.48 | 34.38 | 986.87 | 983.48 | 1244.92 | 29.00 | 1308.30 | 1325.20 | 0.46 | 0.71 |
900/18 | 4 | 31.5 | 18 | 29 | 1.1 | 16:08 | 300 | 1133.2 | 7.5 | 1127.4 | 998.1 | 34.8 | 1071.4 | 21.3 | 0.5 | 16 | 22 | 29.24 | 0.50 | 24.5 | 2.4 | 0.0595 | 0.081 | 1.13 | 0.00 | 0.882 | 0.086 | 2137.28 | 209.081 | 991.33 | 96.98 | 2.40 | 0.24 | 40.4 | 1124.44 | 27.8 | 29 | 7.41 | 34.71 | 996.39 | 992.97 | 1071.42 | 21.28 | 1127.40 | 1133.10 | 0.33 | 0.52 |
900/18 | 5 | 31.5 | 18 | 29 | 1.1 | 16:08 | 300 | 1173.3 | 7.6 | 1165.6 | 999.3 | 34.8 | 1108.0 | 22.8 | 0.6 | 16 | 25 | 29.24 | 0.84 | 23.4 | 2.3 | 0.0628 | 0.086 | 1.13 | 0.00 | 0.849 | 0.083 | 2278.39 | 222.886 | 965.54 | 94.46 | 2.59 | 0.25 | 41.3 | 1137.67 | 27.6 | 29 | 7.43 | 34.80 | 999.10 | 995.67 | 1108.10 | 22.70 | 1165.60 | 1173.20 | 0.36 | 0.55 |
900/18 | 6 | 31.5 | 18 | 29 | 1.1 | 16:08 | 300 | 1152.5 | 7.5 | 1139.9 | 985.4 | 34.3 | 1083.5 | 22.1 | 0.5 | 16 | 28 | 29.24 | 0.42 | 24.9 | 2.4 | 0.0595 | 0.082 | 1.13 | 0.899 | 0.088 | 2146.44 | 209.978 | 993.87 | 97.23 | 2.37 | 0.23 | 39.9 | 1106.15 | 27.7 | 29 | 7.42 | 34.29 | 984.42 | 981.04 | 1083.58 | 22.03 | 1139.90 | 1147.20 | 0.35 | 0.54 |