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Riebesell, Ulf; Revill, Andrew T; Holdsworth, Daniel G; Volkman, J K (2000): Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000. PANGAEA, https://doi.org/10.1594/PANGAEA.727288, Supplement to: Riebesell, U et al. (2000): The effects of varying CO2 concentration on lipid composition and carbon isotope fractionation in Emiliania huxleyi. Geochimica et Cosmochimica Acta, 64(24), 4179-4192, https://doi.org/10.1016/S0016-7037(00)00474-9

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Abstract:
We have measured the stable carbon isotopic composition of bulk organic matter (POC), alkenones, sterols, fatty acids, and phytol in the coccolithophorid Emiliania huxleyi grown in dilute batch cultures over a wide range of CO2 concentrations (1.1-53.5 micromol L-1). The carbon isotope fractionation of POC (POC) varied by ca. 7 per mil and was positively correlated with aqueous CO2 concentration [CO2aq]. While this result confirms general trends observed for the same alga grown in nitrogen-limited chemostat cultures, considerable differences were obtained in absolute values of POC and in the slope of the relationship of POC with growth rate and [CO2aq]. Also, a significantly greater offset was obtained between the delta13C of alkenones and bulk organic matter in this study compared with previous work (5.4, cf. 3.8 per mil). This suggests that the magnitude of the isotope offset may depend on growth conditions. Relative to POC, individual fatty acids were depleted in 13C by 2.3 per mil to 4.1 per mil, phytol was depleted in 13C by 1.9 per mil, and the major sterol 24-methylcholesta-5,22E-dien-3beta-ol was depleted in 13C by 8.5 per mil. This large spread of delta13C values for different lipid classes in the same alga indicates the need for caution in organic geochemical studies when assigning different sources to lipids that might have delta13C values differing by just a few per mil. Increases in [CO2aq] led to dramatic increases in the alkenone contents per cell and as a proportion of organic carbon, but there was no systematic effect on values of U37k- used for reconstructions of paleo sea surface temperature.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Other studied parameter or process; Pelagos; Phytoplankton; Single species
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Event(s):
Riebesell_etal_2000 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Experimental treatmentExp treatRiebesell, Ulf
SalinitySalRiebesell, UlfCTD, Sea-Bird SBE 911plus
Temperature, waterTemp°CRiebesell, UlfCTD, Sea-Bird SBE 911plus
Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
pHpHRiebesell, UlfpH meter, WTW, pH 3000NBS scale
pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
Carbon, inorganic, dissolvedDICµmol/lRiebesell, UlfCoulometric titration
Carbon, inorganic, dissolvedDICµmol/kgRiebesell, UlfCalculatedOriginal values in µmol/l, devided by density
Alkalinity, totalATmmol(eq)/lRiebesell, UlfAlkalinity, Gran titration (Gran, 1950)
10 Alkalinity, totalATµmol/kgRiebesell, UlfCalculatedOriginal values in µmol/l, devided by density
11 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
12 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
13 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
14 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
15 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
16 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
17 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
18 Growth rateµ#/dayRiebesell, UlfCalculated, see reference(s)Daily
19 Growth rateµ#/dayRiebesell, UlfCalculated, see reference(s)Instantaneous
20 δ13C, dissolved inorganic carbonδ13C DIC‰ PDBRiebesell, UlfMass spectrometer Finnigan Delta-S
21 δ13C, carbon dioxide, aquaticδ13C CO2 aqRiebesell, UlfCalculated, see reference(s)
22 δ13C, particulate organic carbonδ13C POC‰ PDBRiebesell, UlfMass spectrometer ANCA-SL 20-20 Europa Scientific
23 Isotopic fractionation, during photosynthisεpRiebesell, UlfCalculated after Freeman & Hayes (1992)
24 Carbon per cellC/cellpmol/#Riebesell, UlfOriginal values in pg, devided by molar mass
25 Nitrogen per cellN/cellpmol/#Riebesell, UlfOriginal values in pg, devided by molar mass
26 Nitrogen/Carbon ratioN/CRiebesell, UlfCalculated
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
286 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Exp treat

Sal
(CTD, Sea-Bird SBE 911plus)

Temp [°C]
(CTD, Sea-Bird SBE 911plus)

CSC flag
(Calculated using seacarb afte...)

pH
(NBS scale, pH meter, WTW, pH ...)

pH
(Total scale, Calculated using...)

DIC [µmol/l]
(Coulometric titration)

DIC [µmol/kg]
(Original values in µmol/l, de...)

AT [mmol(eq)/l]
(Alkalinity, Gran titration (G...)
10 
AT [µmol/kg]
(Original values in µmol/l, de...)
11 
CO2 [µmol/kg]
(Calculated using seacarb afte...)
12 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
13 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
14 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)
15 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
16 
Omega Arg
(Calculated using seacarb afte...)
17 
Omega Cal
(Calculated using seacarb afte...)
18 
µ [#/day]
(Daily, Calculated, see refere...)
19 
µ [#/day]
(Instantaneous, Calculated, se...)
20 
δ13C DIC [‰ PDB]
(Mass spectrometer Finnigan De...)
21 
δ13C CO2 aq []
(Calculated, see reference(s))
22 
δ13C POC [‰ PDB]
(Mass spectrometer ANCA-SL 20-...)
23 
εp []
(Calculated after Freeman & Ha...)
24 
C/cell [pmol/#]
(Original values in pg, devide...)
25 
N/cell [pmol/#]
(Original values in pg, devide...)
26 
N/C
(Calculated)
13220159.128.99212520783.37332990.9528.8228.731147.03930.3114.6822.730.520.85-0.06-9.97-16.907.040.4410.0577.5
13220158.838.70211320672.95528902.3170.4070.161453.03611.219.6514.930.811.32-0.01-9.92-17.307.530.5000.0717.4
13220158.698.57210920632.78727263.41103.79103.441573.22486.017.6711.870.921.490.13-9.78-17.708.050.7660.0938.2
13220158.588.46204720022.59925424.45135.47135.011608.37389.186.149.510.971.570.59-9.32-17.708.490.8490.1008.4
13220158.448.34206020152.50524506.16187.75187.121696.23312.324.937.630.961.560.42-9.49-19.109.810.7490.0938.2
13220158.198.10207920332.352230011.56352.05350.871828.25193.503.054.730.901.460.40-9.51-21.2011.900.8660.0939.4
13220158.198.10208320372.354230211.69356.21355.011833.23192.283.034.700.901.460.36-9.55-21.7012.400.7830.0938.6
23220158.178.09204720022.309225811.78358.79357.591805.13185.092.924.520.821.320.61-9.30-21.4012.300.9490.1218.0
23220158.218.12245824042.779271812.99395.60394.282152.33238.653.775.830.781.26-0.13-10.00-22.0012.300.8830.1217.5
23220158.128.02435142554.742463829.77906.79903.763886.17339.435.368.290.781.27-2.08-12.00-24.4012.700.8330.1147.5
23220158.258.09888486899.739952551.051555.001549.797833.43804.2412.6919.650.761.23-3.30-13.20-26.6013.800.9740.1297.6