Bussmann, Frederik; Giesler, Jakob: Extracellular dissolved organic carbon (DOC) concentrations in axenic and xenic cultures of Thalassiosira gravida in response to varying temperatures and photoperiods [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.992610 (dataset in review)
Abstract:
This dataset comprises dissolved organic carbon (DOC) concentrations from axenic and xenic cultures of Thalassiosira gravida that were cultivated at the Alfred-Wegener-Institute (Bremerhaven, Germany) in March, 2023. After a cell density of ~ 15.000 cells * mL-1 was reached, cultures were filtered through a 0.2 µm polycarbonate (PC) filter (Whatman) that was cleaned by soaking in 10 % hydrochloric acid (HCl, Merck suprapure) for at least 12 h and subsequently rinsing with ultrapure water (Merck Millipore MilliQ). DOC was quantified by high temperature catalytic oxidation with a Shimadzu TOC analyzer (VCPN-TOC, Shimadzu) according to Garzón-Cardona et al. 2024; doi: 10.1016/J.JMARSYS.2023.103893. Cultures were grown under two temperatures (9 °C, 13.5 °C) and two photoperiods (16:8 h, 24:0 h light:dark). The aim was to investigate responses of algal extracellular release and bacterial DOC transformation to marine heatwave-like conditions.
Keyword(s):
Related to:
Bussmann, Frederik; Giesler, Jakob; Koch, Boris P; Kaesler, Jan; Lechtenfeld, Oliver J: Dissolved organic matter (DOM) composition in axenic and xenic cultures of Thalassiosira gravida in response to varying temperatures and photoperiods [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.992625
Garzón Cardona, John E; Martínez, Ana M; Koch, Boris P; Krock, Bernd; Palma, Elbio D; Kong, Xianyu; Lara, Rubén J (2024): Sources and distribution of dissolved organic matter and inorganic nitrogen in waters of the southern Patagonian shelf and northern Drake Passage (51–56°S, 64–69°W). Journal of Marine Systems, 241, 103893, https://doi.org/10.1016/j.jmarsys.2023.103893
Giesler, Jakob; Bussmann, Frederik; Wohlrab, Sylke: Cell densities of the Arctic diatom Thalassiosira gravida in response to temperature, photoperiod and microbiome presence [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.992592
Coverage:
Latitude: 53.534381 * Longitude: 8.580090
Date/Time Start: 2023-03-06T00:00:00 * Date/Time End: 2023-03-22T00:00:00
Event(s):
XeAx23 * Latitude Start: 53.534381 * Longitude Start: 8.580090 * Latitude End: 53.534381 * Longitude End: 8.580090 * Date/Time Start: 2023-03-06T00:00:00 * Date/Time End: 2023-03-22T00:00:00 * Location: Bremerhaven, Germany * Campaign: The_Nansen_Legacy_cruise_707 * Basis: Kronprins Haakon * Comment: Culture isolated in the spring bloom of 2016 at Helgoland Roads; obtained from NORCA culture collection.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Bussmann, Frederik | |||
| 2 | Type of study | Study type | Bussmann, Frederik | Laboratory growth experiment conducted in climate chambers | ||
| 3 | Date/time start, experiment | Date/time start exp | Bussmann, Frederik | |||
| 4 | Date/time end, experiment | Date/time end exp | Bussmann, Frederik | |||
| 5 | Species | Species | Bussmann, Frederik | |||
| 6 | Species, unique identification (URI) | Species UID (URI) | Bussmann, Frederik | |||
| 7 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Bussmann, Frederik | |||
| 8 | Sampling date/time, experiment | Date/time sampling exp | Bussmann, Frederik | Date of sampling day | ||
| 9 | Replicate | Repl | Bussmann, Frederik | Biological replicate | ||
| 10 | Treatment: bacteria | T:bacteria | Bussmann, Frederik | xenic (bacteria containing) or axenic (bacteria-free) diatom culture | ||
| 11 | Treatment: temperature | T:temp | °C | Bussmann, Frederik | ||
| 12 | Treatment: photoperiod | T:photoperiod | h | Bussmann, Frederik | ||
| 13 | Carbon, organic, dissolved | DOC | µmol/l | Bussmann, Frederik | Total organic carbon analyzer (TOC-VCPN), Shimadzu, according to Garzón-Cardona et al., (2024) | Prior to the measurement, cultures were filtered (0.2 µm) and 3.25 mL of sample filtrate were diluted with 3.25 mL of ultrapure water. |
| 14 | Sample ID | Sample ID | Bussmann, Frederik | ID of filtered culture given in the sample management system at the Ecological Chemistry Section, Alfred-Wegener-Institute (Bremerhaven, Germany) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
480 data points