Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Bussmann, Frederik; Giesler, Jakob; Koch, Boris P; Kaesler, Jan; Lechtenfeld, Oliver J: Dissolved organic matter (DOM) composition in axenic and xenic cultures of Thalassiosira gravida in response to varying temperatures and photoperiods [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.992625 (dataset in review)

ShareShow MapGoogle Earth

Abstract:
This dataset comprises dissolved organic matter (DOM) composition from axenic and xenic cultures of Thalassiosira gravida that were cultivated at the Alfred-Wegener-Institute (Bremerhaven, Germany) in March, 2023. After a cell density of ~ 15.000 cells * mL-1 was reached, cultures were filtered through a 0.2 µm polycarbonate (PC) filter (Whatman) that was cleaned by soaking in 10 % hydrochloric acid (HCl, Merck suprapure) for at least 12 h and subsequently rinsing with ultrapure water (Merck Millipore MilliQ). Cultures were grown under two temperatures (9 °C, 13.5 °C) and two photoperiods (16:8 h, 24:0 h light:dark). 2 mL of the sample were filtered through a 0.2 µm regenerated cellulose (RC)-membrane syringe filter (Sartorius) after defrosting. Molecular composition data were acquired with Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) coupled to a reversed-phase liquid chromatography (RPLC) with negative electrospray ionization (ESI) according to Lechtenfeld et al., 2024 (doi: 10.1021/acs.est.3c07219). Measurements were performed on on a solariX XR, Bruker Daltonics, Billerica, U.S.A. at the Helmholtz Centre for Environmental Research (UFZ; Leipzig, Germany). Molecular formulas were assigned and filtered using UltraMassExplorer (Leefmann et., 2019; doi: 10.1002/rcm.8315). If the Total ion chromatogram (TIC) was much higher and/or different in certain retention time windows compared to other samples of the same treatment, the sample was excluded from the dataset. The aim of this study was to investigate responses of algal extracellular release and bacterial DOM transformation to marine heatwave-like conditions.
Keyword(s):
culture experiment; Dissolved Organic Matter; Fourier transform ion cyclotron resonance mass spectrometry; molecular composition; photoperiod; Temperature; Thalassiosira sp.
Related to:
Bussmann, Frederik; Giesler, Jakob: Extracellular dissolved organic carbon (DOC) concentrations in axenic and xenic cultures of Thalassiosira gravida in response to varying temperatures and photoperiods [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.992610
Lechtenfeld, Oliver J; Kaesler, Jan; Jennings, Elaine K; Koch, Boris P (2024): Direct Analysis of Marine Dissolved Organic Matter Using LC-FT-ICR MS. Environmental Science & Technology, 58(10), 4637-4647, https://doi.org/10.1021/acs.est.3c07219
Leefmann, Tim; Frickenhaus, Stephan; Koch, Boris P (2019): UltraMassExplorer: a browser-based application for the evaluation of high-resolution mass spectrometric data. Rapid Communications in Mass Spectrometry, 33(2), 193-202, https://doi.org/10.1002/rcm.8315
Coverage:
Latitude: 53.534381 * Longitude: 8.580090
Date/Time Start: 2023-03-06T00:00:00 * Date/Time End: 2023-03-22T00:00:00
Event(s):
XeAx23 * Latitude Start: 53.534381 * Longitude Start: 8.580090 * Latitude End: 53.534381 * Longitude End: 8.580090 * Date/Time Start: 2023-03-06T00:00:00 * Date/Time End: 2023-03-22T00:00:00 * Location: Bremerhaven, Germany * Campaign: The_Nansen_Legacy_cruise_707 * Basis: Kronprins Haakon * Comment: Culture isolated in the spring bloom of 2016 at Helgoland Roads; obtained from NORCA culture collection.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventBussmann, Frederik
2Type of studyStudy typeBussmann, FrederikLaboratory growth experiment conducted in climate chambers
3Date/time start, experimentDate/time start expBussmann, Frederik
4Date/time end, experimentDate/time end expBussmann, Frederik
5SpeciesSpeciesBussmann, Frederik
6Species, unique identificationSpecies UIDBussmann, Frederik
7Sampling date/time, experimentDate/time sampling expBussmann, FrederikDate of sampling day
8ReplicateReplBussmann, FrederikBiological replicate
9Treatment: bacteriaT:bacteriaBussmann, Frederikxenic (bacteria containing) or axenic (bacteria-free) diatom culture
10Treatment: temperatureT:temp°CBussmann, Frederik
11Treatment: photoperiodT:photoperiodhBussmann, Frederik
12Sample code/labelSample labelBussmann, Frederik
13Sample IDSample IDBussmann, FrederikID of filtered culture given in the sample management system at the Ecological Chemistry Section, Alfred-Wegener-Institute (Bremerhaven, Germany)
14Molecular formulaFormulaBussmann, FrederikFourier-transform ion cyclotron resonance mass spectrometry, solariX XR Bruker Daltonics; coupled to liqued chromatography (LC-FT-ICR-MS) and negative electrospray ionization (ESI), Apollo II, Bruker Daltonics, according to Lechtenfeld et al. (2024) (RPLC-FT-ICR-MS)Molecular formulas were assigned using UltraMassExplorer (Leefmann et., 2019; doi: 10.1002/rcm.8315)
15Retention timeRTminBussmann, FrederikFourier-transform ion cyclotron resonance mass spectrometry, solariX XR Bruker Daltonics; coupled to liqued chromatography (LC-FT-ICR-MS) and negative electrospray ionization (ESI), Apollo II, Bruker Daltonics, according to Lechtenfeld et al. (2024) (RPLC-FT-ICR-MS)The dataset was divided into retention time segments (one-minute intervals) from 12.5 min to 25.5 min. Each retention time segment was recalibrated internally.
16Mass accuracyMass accppmBussmann, FrederikFourier-transform ion cyclotron resonance mass spectrometry, solariX XR Bruker Daltonics; coupled to liqued chromatography (LC-FT-ICR-MS) and negative electrospray ionization (ESI), Apollo II, Bruker Daltonics, according to Lechtenfeld et al. (2024) (RPLC-FT-ICR-MS)
17Mass-to-charge ratiom/zBussmann, FrederikFourier-transform ion cyclotron resonance mass spectrometry, solariX XR Bruker Daltonics; coupled to liqued chromatography (LC-FT-ICR-MS) and negative electrospray ionization (ESI), Apollo II, Bruker Daltonics, according to Lechtenfeld et al. (2024) (RPLC-FT-ICR-MS)
18Mass peak magnitudeMagnitudearbitrary unitsBussmann, FrederikFourier-transform ion cyclotron resonance mass spectrometry, solariX XR Bruker Daltonics; coupled to liqued chromatography (LC-FT-ICR-MS) and negative electrospray ionization (ESI), Apollo II, Bruker Daltonics, according to Lechtenfeld et al. (2024) (RPLC-FT-ICR-MS)
19Signal/noise ratioSNRBussmann, FrederikFourier-transform ion cyclotron resonance mass spectrometry, solariX XR Bruker Daltonics; coupled to liqued chromatography (LC-FT-ICR-MS) and negative electrospray ionization (ESI), Apollo II, Bruker Daltonics, according to Lechtenfeld et al. (2024) (RPLC-FT-ICR-MS)
20ResolutionResolutionBussmann, FrederikFourier-transform ion cyclotron resonance mass spectrometry, solariX XR Bruker Daltonics; coupled to liqued chromatography (LC-FT-ICR-MS) and negative electrospray ionization (ESI), Apollo II, Bruker Daltonics, according to Lechtenfeld et al. (2024) (RPLC-FT-ICR-MS)
21Molecular mass, neutralN massg/molBussmann, FrederikFourier-transform ion cyclotron resonance mass spectrometry, solariX XR Bruker Daltonics; coupled to liqued chromatography (LC-FT-ICR-MS) and negative electrospray ionization (ESI), Apollo II, Bruker Daltonics, according to Lechtenfeld et al. (2024) (RPLC-FT-ICR-MS)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
377740 data points

Download Data (login required; moratorium until 2026-08-25)

Download dataset as tab-delimited text — use the following character encoding:

View dataset as HTML (shows only first 2000 rows)