Giesler, Jakob; Bussmann, Frederik; Wohlrab, Sylke: Cell densities of the Arctic diatom Thalassiosira gravida in response to temperature, photoperiod and microbiome presence [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.992592 (dataset in review)
Abstract:
In March 2023, cell densities of the Arctic diatom Thalassiosira gravida (isolated from the Central Arctic Ocean) were determined to calculate its growth rates at different temperatures and photoperiods in the presence and absence of its natural microbiome. Therefore, a full-factorial experimental design was chosen with two levels of temperature (9°C; 13.5°C) and photoperiod (16h; 24h), to which axenic and xenic diatom cultures were acclimated for one week in climate cabinets prior to the start of the actual growth experiment at a light intensity of 50 µmol photons m-2 s-1. With an initial cell density of 1500 cells/ml, axenic and xenic diatoms were grown under the respective experimental conditions until a cell density of approximately 15000 cells/ml was reached. Cell densities were determined microscopically using an inverted light microscope, following the procedure described in detail in Giesler et al. (2023, 10.3389/fmars.2023.1244639).
Related to:
Bussmann, Frederik; Giesler, Jakob: Extracellular dissolved organic carbon (DOC) concentrations in axenic and xenic cultures of Thalassiosira gravida in response to varying temperatures and photoperiods [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.992610
Project(s):
Changing Earth – Sustaining our Future (Helmholtz_ChangingEarth)
Coverage:
Latitude: 53.534381 * Longitude: 8.580090
Date/Time Start: 2023-03-06T00:00:00 * Date/Time End: 2023-03-22T00:00:00
Event(s):
XeAx23 * Latitude Start: 53.534381 * Longitude Start: 8.580090 * Latitude End: 53.534381 * Longitude End: 8.580090 * Date/Time Start: 2023-03-06T00:00:00 * Date/Time End: 2023-03-22T00:00:00 * Location: Bremerhaven, Germany * Campaign: The_Nansen_Legacy_cruise_707 * Basis: Kronprins Haakon * Comment: Culture isolated in the spring bloom of 2016 at Helgoland Roads; obtained from NORCA culture collection.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Giesler, Jakob | |||
| 2 | Type of study | Study type | Giesler, Jakob | Laboratory growth experiment conducted in climate chambers | ||
| 3 | Date/time start, experiment | Date/time start exp | Giesler, Jakob | |||
| 4 | Date/time end, experiment | Date/time end exp | Giesler, Jakob | |||
| 5 | Species | Species | Giesler, Jakob | |||
| 6 | Species, unique identification (URI) | Species UID (URI) | Giesler, Jakob | |||
| 7 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Giesler, Jakob | |||
| 8 | Experiment number ID | Exp no ID | Giesler, Jakob | ID of particular culture flask in the experiment | ||
| 9 | Sampling date/time, experiment | Date/time sampling exp | Giesler, Jakob | Date of sampling day | ||
| 10 | Day of experiment | DOE | day | Giesler, Jakob | Experimental sampling day | |
| 11 | Sample ID | Sample ID | Giesler, Jakob | Official strain ID in the culture collections of the University of Bremen | ||
| 12 | Replicate | Repl | Giesler, Jakob | Biological replicate | ||
| 13 | Treatment: bacteria | T:bacteria | Giesler, Jakob | xenic (bacteria containing) or axenic (bacteria-free) diatom culture | ||
| 14 | Treatment: temperature | T:temp | °C | Giesler, Jakob | ||
| 15 | Treatment: photoperiod | T:photoperiod | h | Giesler, Jakob | ||
| 16 | Cell density | Cells | #/ml | Giesler, Jakob | Counted using an inverted light microscopy, Zeiss, Axiovert 10C, according to Giesler et al., 2023 |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1120 data points