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Hellige, Inga; Akeerath Mundanatt, Aman: Polysaccharide fucoidan BAM1 and arabinogalactan-protein glycan JIM13 antibody binding in sediment cores from coastal vegetated ecosystems [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.979912 (dataset in review)

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Abstract:
50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. For more specific analysis enzyme-linked immunosorbent assay (ELISA) was used for detection of fucoidan and arabinogalactan-protein glycan as described in the following studies (Vidal-Melgosa et al., 2021 and Cornuault et al., 2014). In short, 100 µL of sediment extracts were added to a pre-coated 96-well plate and incubated overnight at 4°C. The signal was developed using primary antibodies, BAM1 (fucoidan) and JIM13 (arabinogalactan-protein glycan), diluted 1:10 in skim milk PBS solution, followed by anti-rat antibody at a 1:1000 dilution in the same solution. Absorbance was measured at 450 nm using a Spectramax Id3 plate reader (Molecular Devices).
Keyword(s):
carbon dioxide removal; coastal vegetated ecosystems; Glycans; marine carbon sequestration
Supplement to:
Akeerath Mundanatt, Aman; Massing, Jana Chiara; Hehemann, Jan-Hendrik; Hellige, Inga (in prep.): Roots of coastal plants stabilize carbon fixed by marine algae.
References:
Cornuault, Valérie; Manfield, Iain W; Ralet, Marie‐Christine; Knox, J Paul (2014): Epitope detection chromatography: a method to dissect the structural heterogeneity and inter‐connections of plant cell‐wall matrix glycans. Plant Journal, 78(4), 715-722, https://doi.org/10.1111/tpj.12504
Vidal-Melgosa, Silvia; Sichert, Andreas; Francis, T Ben; Bartosik, Daniel; Niggemann, Jutta; Wichels, Antje; Willats, William G T; Fuchs, Bernhard M; Teeling, Hanno; Becher, Dörte; Schweder, Thomas; Amann, Rudolf; Hehemann, Jan-Hendrik (2021): Diatom fucan polysaccharide precipitates carbon during algal blooms. Nature Communications, 12(1), https://doi.org/10.1038/s41467-021-21009-6
Project(s):
DAM CDRmare - sea4soCiety: Searching for solutions for Carbon-sequestration in coastal ecosystems (sea4soCiety)
Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways (CDRmare)
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03F0896E: DAM CDRmare - sea4soCiety, University of Bremen
Coverage:
Median Latitude: 32.411781 * Median Longitude: 13.507392 * South-bound Latitude: 4.660548 * West-bound Longitude: -75.682996 * North-bound Latitude: 54.692271 * East-bound Longitude: 103.439555
Date/Time Start: 2021-11-08T00:00:00 * Date/Time End: 2022-10-11T00:00:00
Minimum DEPTH, sediment/rock: 0.007 m * Maximum DEPTH, sediment/rock: 0.485 m
Event(s):
GER_HAH_SG_P4_SC * Latitude: 54.612684 * Longitude: 8.823788 * Date/Time: 2021-11-09T00:00:00 * Campaign: s4s_GER-Land_2021_NorthSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
GER_HAH_SG_P6_SC * Latitude: 54.612663 * Longitude: 8.823431 * Date/Time: 2021-11-09T00:00:00 * Campaign: s4s_GER-Land_2021_NorthSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
GER_HAH_SG_P8_SC * Latitude: 54.612680 * Longitude: 8.822986 * Date/Time: 2021-11-09T00:00:00 * Campaign: s4s_GER-Land_2021_NorthSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventHellige, IngaUniversal label
2Sample code/labelSample labelHellige, Inga
3CountryCountryHellige, Inga
4LocationLocationHellige, Inga
5EcosystemEcosystemHellige, Inga
6LONGITUDELongitudeHellige, IngaGeocode
7LATITUDELatitudeHellige, IngaGeocode
8DATE/TIMEDate/TimeHellige, IngaGeocode
9IdentificationIDHellige, IngaPoint
10Depth commentDepth commHellige, IngaDepth range in cm
11DEPTH, sediment/rockDepth sedmHellige, IngaGeocode – mean
12Depth, sediment/rock, top/minimumDepth sed topmHellige, Inga
13Depth, sediment/rock, bottom/maximumDepth sed botmHellige, Inga
14Layer numberLayerHellige, IngaDepth layer
15Layer descriptionLayerHellige, Inga
16CommentCommentHellige, Inga
17Polysaccharides, relative BAM1 antibody signalBAM1Hellige, Inga
18Arabinogalactan protein glycan, relative JIM13 antibody signalJIM13Hellige, Inga
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3060 data points

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