Citation:

PANGAEA.975016 (DOI registration in progress)

Name: Monosaccharides quantified in porewater samples from coastal vegetated ecosystems
Published: 2025-04-08
License: https://creativecommons.org/licenses/by/4.0/
Keywords: carbon dioxide removal; coastal vegetated ecosystems; Glycans; marine carbon sequestration

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Published: 2025-04-08

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Abstract:

Porewater was taken from 30 to 50 cm depths in saltmarsh, seagrass and unvegetated areas around the German Bight in 2022 and 2023. Up to 9 points per ecosystem were sampled along a transect. Polysaccharides >5kDa were upconcentrated using AMICON-filtration devide and afterwards freeze dried. Dired samples were resuspended in MilliQ-water and acid hydrolysed (1 M HCl, 24 h, 100°C). Monosaccharides were analysed using anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD, ThermoFisher Dionex ICS-5000+ system equipped with a CarboPac PA10 analytical column (2 x 250 mm) and a CarboPac PA10 guard column (2 x 50 mm)), according to Engel et al. (2011).

Keyword(s):

carbon dioxide removal; coastal vegetated ecosystems; Glycans; marine carbon sequestration

Supplement to:

Akeerath Mundanatt, Aman; Massing, Jana Chiara; Hehemann, Jan-Hendrik; Hellige, Inga (in prep.): Roots of coastal plants stabilize carbon fixed by marine algae.

References:

Engel, Anja; Händel, Nicole (2011): A novel protocol for determining the concentration and composition of sugars in particulate and in high molecular weight dissolved organic matter (HMW-DOM) in seawater. Marine Chemistry, 127(1-4), 180-191, https://doi.org/10.1016/j.marchem.2011.09.004

Project(s):

DAM CDRmare - sea4soCiety: Searching for solutions for Carbon-sequestration in coastal ecosystems (sea4soCiety)

Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways (CDRmare)

Funding:

Federal Ministry of Education and Research (BMBF), grant/award no. 03F0896E : DAM CDRmare - sea4soCiety, University of Bremen

Coverage:

Median Latitude: 54.315485 * Median Longitude: 9.554442 * South-bound Latitude: 53.684215 * West-bound Longitude: 7.334821 * North-bound Latitude: 54.692271 * East-bound Longitude: 11.025222

Date/Time Start: 2021-11-10T00:00:00 * Date/Time End: 2023-08-29T00:00:00

Minimum DEPTH, sediment/rock: 0.5 m * Maximum DEPTH, sediment/rock: 0.5 m

Event(s):

GER_HEI_SG_P1_PW * Latitude: 54.380190 * Longitude: 11.025222 * Date/Time: 2022-08-24T00:00:00 * Campaign: * Basis: Sampling on land * Method/Device: Porewater lance

GER_HEI_SG_P2_PW * Latitude: 54.380216 * Longitude: 11.025067 * Date/Time: 2022-08-24T00:00:00 * Campaign: * Basis: Sampling on land * Method/Device: Porewater lance

GER_HEI_SG_P3_PW * Latitude: 54.380240 * Longitude: 11.024932 * Date/Time: 2022-08-24T00:00:00 * Campaign: * Basis: Sampling on land * Method/Device: Porewater lance

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Hellige, Inga		Universal label
2	LONGITUDE	Longitude		Hellige, Inga		Geocode
3	LATITUDE	Latitude		Hellige, Inga		Geocode
4	DEPTH, sediment/rock	Depth sed	m	Hellige, Inga		Geocode
5	DATE/TIME	Date/Time		Hellige, Inga		Geocode
6	Country	Country		Hellige, Inga		GER - Germany
7	Location	Location		Hellige, Inga		MET - Mettgrund, NES - Nessmersiel, WEN - Wendtorf, SCH - Schleimünde, HEI - Heiligenhafen
8	Ecosystem	Ecosystem		Hellige, Inga		UV - unvegetated area, SG - seagrass, SM - saltmarsh
9	Identification	ID		Hellige, Inga		Point
10	Comment	Comment		Hellige, Inga
11	Fucose	Fuc	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
12	Rhamnose	Rha	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
13	Galactosamine	GalN	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
14	Arabinose	Ara	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
15	Glucosamine	GlcN	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
16	Galactose	Gal	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
17	Glucose	Glc	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
18	Mannose	Man	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
19	Xylose	Xyl	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
20	Gluconic acid	GlucAc	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
21	Muramic acid	Mur	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
22	Galacturonic acid	GalA	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
23	Glucuronic acid	GlcA	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
24	Mannuronic acid	ManA	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+
25	Iduronic acid	IdoA	µg/l	Hellige, Inga	Ion chromatograph, Thermo Scientific, Dionex ICS-5000+

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1916 data points

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