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Hellige, Inga; Akeerath Mundanatt, Aman: Polysaccharides quantified in sediment cores from coastal vegetated ecosystems [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.979911 (dataset in review)

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Abstract:
50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for quantification of total carbohydrates. Polysaccharide were screened using microarray analysis according to Vidal-Melgosa et. al, 2022. For more specific analysis enzyme-linked immunosorbent assay (ELISA) was used for detection of fucoidan and arabinogalactan-protein glycan as described in the following studies (Vidal-Melgosa et al., 2021 and Cornuault et al., 2014). Extracts were acid hydrolysed (1 M HCl, 24 h, 100°C) and monosaccharides were analysed using anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), according to Engel et al., 2011.
Keyword(s):
carbon dioxide removal; coastal vegetated ecosystems; Glycans; marine carbon sequestration
Supplement to:
Akeerath Mundanatt, Aman; Massing, Jana Chiara; Hehemann, Jan-Hendrik; Hellige, Inga (in prep.): Roots of coastal plants stabilize carbon fixed by marine algae.
Related to:
Cornuault, Valérie; Manfield, Iain W; Ralet, Marie‐Christine; Knox, J Paul (2014): Epitope detection chromatography: a method to dissect the structural heterogeneity and inter‐connections of plant cell‐wall matrix glycans. Plant Journal, 78(4), 715-722, https://doi.org/10.1111/tpj.12504
Engel, Anja; Händel, Nicole (2011): A novel protocol for determining the concentration and composition of sugars in particulate and in high molecular weight dissolved organic matter (HMW-DOM) in seawater. Marine Chemistry, 127(1-4), 180-191, https://doi.org/10.1016/j.marchem.2011.09.004
Vidal-Melgosa, Silvia; Lagator, Matija; Sichert, Andreas; Priest, Taylor; Pätzold, Jürgen; Hehemann, Jan-Hendrik (preprint): Not digested: algal glycans move carbon dioxide into the deep-sea. bioRxiv, 2022.03.04.483023v1, https://doi.org/10.1101/2022.03.04.483023
Vidal-Melgosa, Silvia; Sichert, Andreas; Francis, T Ben; Bartosik, Daniel; Niggemann, Jutta; Wichels, Antje; Willats, William G T; Fuchs, Bernhard M; Teeling, Hanno; Becher, Dörte; Schweder, Thomas; Amann, Rudolf; Hehemann, Jan-Hendrik (2021): Diatom fucan polysaccharide precipitates carbon during algal blooms. Nature Communications, 12(1), https://doi.org/10.1038/s41467-021-21009-6
Project(s):
DAM CDRmare - sea4soCiety: Searching for solutions for Carbon-sequestration in coastal ecosystems (sea4soCiety)
Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways (CDRmare)
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03F0896E: DAM CDRmare - sea4soCiety, University of Bremen
Coverage:
Median Latitude: 29.114258 * Median Longitude: -39.169180 * South-bound Latitude: 10.136577 * West-bound Longitude: -75.682996 * North-bound Latitude: 54.692271 * East-bound Longitude: 11.021790
Date/Time Start: 2021-11-08T13:55:06 * Date/Time End: 2022-08-30T10:43:00
Minimum DEPTH, sediment/rock: 0.022 m * Maximum DEPTH, sediment/rock: 0.465 m
Event(s):
GER_HAH_SM_P7_SC * Latitude: 54.605339 * Longitude: 8.824193 * Date/Time: 2021-11-08T00:00:00 * Campaign: s4s_GER-Land_2021_NorthSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
GER_HEI_SM_P1_SC * Latitude: 54.377104 * Longitude: 11.021790 * Date/Time: 2022-08-23T00:00:00 * Campaign: s4s_GER-Land_2022_BalticSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
GER_HEI_SM_P3_SC * Latitude: 54.379271 * Longitude: 11.020723 * Date/Time: 2022-08-23T00:00:00 * Campaign: s4s_GER-Land_2022_BalticSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventHellige, IngaUniversal label
2Sample code/labelSample labelHellige, Inga
3CountryCountryHellige, IngaGER - Germany
4LocationLocationHellige, Inga
5EcosystemEcosystemHellige, Inga
6Depth commentDepth commHellige, IngaDepth range in cm
7DEPTH, sediment/rockDepth sedmHellige, IngaGeocode – mean
8Depth, sediment/rock, top/minimumDepth sed topmHellige, Inga
9Depth, sediment/rock, bottom/maximumDepth sed botmHellige, Inga
10LONGITUDELongitudeHellige, IngaGeocode
11LATITUDELatitudeHellige, IngaGeocode
12DATE/TIMEDate/TimeHellige, IngaGeocode
13Fucan, sulfated epitope, relative BAM2 antibody signalBAM2Hellige, Inga
14Fucan, sulfated epitope, relative BAM4 antibody signalBAM4Hellige, Inga
15Homogalacturonan, partially methyl-esterified/de-esterified, relative JIM5 antibody signalJIM5Hellige, Inga
16Homogalacturonan, methyl-esterified, relative JIM7 antibody signalJIM7Hellige, Inga
17Rhamnogalacturonan I backbone, relative INRA-RU1 antibody signalINRA-RU1Hellige, Inga
18beta-1,4-D-galactan, relative LM5 antibody signalLM5Hellige, Inga
19alpha-1,5-L-arabinan, relative LM6 antibody signalLM6Hellige, Inga
20alpha-1,5-L-arabinan, relative LM6-M antibody signalLM6-MHellige, Inga
21beta-1,3-D-glucan, relative BS-400-2 antibody signalBS-400-2Hellige, Inga
22Grass xylan, relative LM27 antibody signalLM27Hellige, Inga
23Arabinogalactan protein glycan, relative JIM8 antibody signalJIM8Hellige, Inga
24Arabinogalactan protein glycan, relative JIM13 antibody signalJIM13Hellige, Inga
25Arabinogalactan protein glycan, relative MAC207 antibody signalMAC207Hellige, Inga
26Alginate mannuronate-guluronate, relative BAM7 antibody signalBAM7Hellige, Inga
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
782 data points

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