Hellige, Inga; Akeerath Mundanatt, Aman; Fett, Theresa-Marie; Wölfelschneider, Mirco; Prugger, Jennifer; Geuer, Jana K; Reents, Svenja (2025): Polysaccharides quantified in sediment cores from coastal vegetated ecosystems [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.979911 (DOI registration in progress)
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Published: 2025-04-28
Abstract:
50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. Polysaccharides were screened using microarray analysis following the method described by Vidal-Melgosa et al. (2022). Briefly, sediment extracts from MilliQ-water and EDTA were combined in equal volumes, and 30 µL of the mixture was transferred into wells of 384-microwell plates. Two consecutive two-fold dilutions were performed using a printing buffer (55.2% glycerol, 44% water, 0.8% Triton X-100). The plates were then centrifuged at 3,500 × g for 10 minutes at 15 °C. Each microarray was individually probed with a monoclonal antibody (mAb), and binding was detected using a secondary antibody conjugated to alkaline phosphatase. In the presence of its substrate, this reaction produced a colorimetric signal. Developed arrays were scanned at 2400 dots per inch, and binding signal intensity was quantified using Array-Pro Analyzer 6.3 software (Media Cybernetics).
Keyword(s):
Supplement to:
Akeerath Mundanatt, Aman; Massing, Jana Chiara; Hehemann, Jan-Hendrik; Hellige, Inga (in prep.): Roots of coastal plants stabilize carbon fixed by marine algae.
References:
Vidal-Melgosa, Silvia; Lagator, Matija; Sichert, Andreas; Priest, Taylor; Pätzold, Jürgen; Hehemann, Jan-Hendrik (preprint): Not digested: algal glycans move carbon dioxide into the deep-sea. bioRxiv, 2022.03.04.483023v1, https://doi.org/10.1101/2022.03.04.483023
Project(s):
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03F0896E: DAM CDRmare - sea4soCiety, University of Bremen
Coverage:
Median Latitude: 29.114258 * Median Longitude: -39.169180 * South-bound Latitude: 10.136577 * West-bound Longitude: -75.682996 * North-bound Latitude: 54.692271 * East-bound Longitude: 11.021790
Date/Time Start: 2021-11-08T00:00:00 * Date/Time End: 2022-08-30T00:00:00
Minimum DEPTH, sediment/rock: 0.022 m * Maximum DEPTH, sediment/rock: 0.465 m
Event(s):
GER_HAH_SM_P7_SC * Latitude: 54.605339 * Longitude: 8.824193 * Date/Time: 2021-11-08T00:00:00 * Campaign: s4s_GER-Land_2021_NorthSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
GER_HEI_SM_P1_SC * Latitude: 54.377104 * Longitude: 11.021790 * Date/Time: 2022-08-23T00:00:00 * Campaign: s4s_GER-Land_2022_BalticSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
GER_HEI_SM_P3_SC * Latitude: 54.379271 * Longitude: 11.020723 * Date/Time: 2022-08-23T00:00:00 * Campaign: s4s_GER-Land_2022_BalticSea * Basis: Sampling on land * Method/Device: Peat corer (PEATC)
Comment:
The samples were analysed on 2024-10-09 including the standards for the microarray analysis.
Parameter(s):
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
782 data points