Hellige, Inga; Baiko, Dariya; Geuer, Jana K; Imai, Bruna: Polysaccharide fucoidan BAM1 antibody binding in porewater samples from coastal vegetated ecosystems [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.977549 (dataset in review)
Abstract:
Porewater of saltmarsh, seagrass and unvegetated areas around the German Bight in 2022 and 2023 was sampled at 50cm depth. A transect of up to 9 points per ecosystem were sampled. Polysaccharides >5kDa were upconcentrated using AMICON-filtration device, freeze dried and dried samples were resuspended in MilliQ-water. Polysaccharides were screened for fucoidan BAM1 antibody binding using ELISA method, according to Cornuault et al. (2014).
Keyword(s):
Supplement to:
Akeerath Mundanatt, Aman; Massing, Jana Chiara; Hehemann, Jan-Hendrik; Hellige, Inga (in prep.): Roots of coastal plants stabilize carbon fixed by marine algae.
References:
Cornuault, Valérie; Manfield, Iain W; Ralet, Marie‐Christine; Knox, J Paul (2014): Epitope detection chromatography: a method to dissect the structural heterogeneity and inter‐connections of plant cell‐wall matrix glycans. Plant Journal, 78(4), 715-722, https://doi.org/10.1111/tpj.12504
Project(s):
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03F0896E: DAM CDRmare - sea4soCiety, University of Bremen
Coverage:
Median Latitude: 54.283429 * Median Longitude: 9.553794 * South-bound Latitude: 53.684215 * West-bound Longitude: 7.334766 * North-bound Latitude: 54.692271 * East-bound Longitude: 11.025222
Date/Time Start: 2021-11-10T00:00:00 * Date/Time End: 2023-08-29T00:00:00
Minimum DEPTH, sediment/rock: 0.5 m * Maximum DEPTH, sediment/rock: 0.5 m
Event(s):
GER_HEI_SG_P1_PW * Latitude: 54.380190 * Longitude: 11.025222 * Date/Time: 2022-08-24T00:00:00 * Campaign: s4s_GER-Land_2022_BalticSea * Basis: Sampling on land * Method/Device: Porewater lance
GER_HEI_SG_P2_PW * Latitude: 54.380216 * Longitude: 11.025067 * Date/Time: 2022-08-24T00:00:00 * Campaign: s4s_GER-Land_2022_BalticSea * Basis: Sampling on land * Method/Device: Porewater lance
GER_HEI_SG_P3_PW * Latitude: 54.380240 * Longitude: 11.024932 * Date/Time: 2022-08-24T00:00:00 * Campaign: s4s_GER-Land_2022_BalticSea * Basis: Sampling on land * Method/Device: Porewater lance
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Hellige, Inga | Universal_label | ||
| 2 | LONGITUDE | Longitude | Hellige, Inga | Geocode | ||
| 3 | LATITUDE | Latitude | Hellige, Inga | Geocode | ||
| 4 | DEPTH, sediment/rock | Depth sed | m | Hellige, Inga | Geocode | |
| 5 | DATE/TIME | Date/Time | Hellige, Inga | Geocode | ||
| 6 | Country | Country | Hellige, Inga | GER - Germany | ||
| 7 | Location | Location | Hellige, Inga | MET - Mettgrund, NES - Nessmersiel, WEN - Wendtorf, SCH - Schleimünde, HEI - Heiligenhafen | ||
| 8 | Ecosystem | Ecosystem | Hellige, Inga | UV - unvegetated area, SG - seagrass, SM - saltmarsh | ||
| 9 | Identification | ID | Hellige, Inga | Point | ||
| 10 | Polysaccharides, relative BAM1 antibody signal | BAM1 | Hellige, Inga | Enzyme-linked immunosorbent assay (ELISA) according to Cornuault et al. (2014) | Relative signal (BAM1) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
490 data points
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Datasets with similar metadata
- Hellige, I; Baiko, D; Geuer, JK et al. (in review): Monosaccharides quantified in porewater samples from coastal vegetated ecosystems. https://doi.pangaea.de/10.1594/PANGAEA.975016
- Hellige, I; Akeerath Mundanatt, A (in review): Monosaccharides quantified in sediment cores from coastal vegetated ecosystems. https://doi.pangaea.de/10.1594/PANGAEA.979909
- Hellige, I; Akeerath Mundanatt, A (in review): Polysaccharides quantified in sediment cores from coastal vegetated ecosystems. https://doi.pangaea.de/10.1594/PANGAEA.979911