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Thor, Peter; Vermandele, Fanny; Bailey, Allison; Guscelli, Ella; Loubet‑Sartrou, Léa; Dupont, Sam; Calosi, Piero (2022): Seawater carbonate chemistry and cellular metabolism of the Arctic copepod Calanus glacialis [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.957185

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Abstract:
Using a targeted metabolomic approach we investigated the effects of low seawater pH on energy metabolism in two late copepodite stages (CIV and CV) of the keystone Arctic copepod species Calanus glacialis. Exposure to decreasing seawater pH (from 8.0 to 7.0) caused increased ATP, ADP and NAD+ and decreased AMP concentrations in stage CIV, and increased ATP and phospho-L-arginine and decreased AMP concentrations in stage CV. Metabolic pathway enrichment analysis showed enrichment of the TCA cycle and a range of amino acid metabolic pathways in both stages. Concentrations of lactate, malate, fumarate and alpha-ketoglutarate (all involved in the TCA cycle) increased in stage CIV, whereas only alpha-ketoglutarate increased in stage CV. Based on the pattern of concentration changes in glucose, pyruvate, TCA cycle metabolites, and free amino acids, we hypothesise that ocean acidification will lead to a shift in energy production from carbohydrate metabolism in the glycolysis toward amino acid metabolism in the TCA cycle and oxidative phosphorylation in stage CIV. In stage CV, concentrations of most of the analysed free fatty acids increased, suggesting in particular that ocean acidification increases the metabolism of stored wax esters in this stage. Moreover, aminoacyl-tRNA biosynthesis was enriched in both stages indicating increased enzyme production to handle low pH stress.
Keyword(s):
Animalia; Arctic; Arthropoda; Bottles or small containers/Aquaria (<20 L); Calanus glacialis; Coast and continental shelf; Laboratory experiment; Other studied parameter or process; Pelagos; Polar; Single species; Zooplankton
Supplement to:
Thor, Peter; Vermandele, Fanny; Bailey, Allison; Guscelli, Ella; Loubet‑Sartrou, Léa; Dupont, Sam; Calosi, Piero (2022): Ocean acidification causes fundamental changes in the cellular metabolism of the Arctic copepod Calanus glacialis as detected by metabolomic analysis. Scientific Reports, 12(1), 22223, https://doi.org/10.1038/s41598-022-26480-9
Documentation:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Coverage:
Latitude: 79.000000 * Longitude: 11.700000
Event(s):
Kongsfjord_copepod * Latitude: 79.000000 * Longitude: 11.700000 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-03.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeThor, PeterStudy
2Species, unique identificationSpecies UIDThor, Peter
3Species, unique identification (URI)Species UID (URI)Thor, Peter
4Species, unique identification (Semantic URI)Species UID (Semantic URI)Thor, Peter
5StageStageThor, Peter
6pHpHThor, PeterTotal scale
7MetaboliteMetabolThor, PeterATP
8MetaboliteMetabolThor, PeterADP
9MetaboliteMetabolThor, PeterAMP
10MetaboliteMetabolThor, PeterNAD, data in line 1 and 31 are outlier
11MetaboliteMetabolThor, PeterGlucose
12MetaboliteMetabolThor, PeterLactate
13MetaboliteMetabolThor, PeterPyruvate
14MetaboliteMetabolThor, PeterFumarate
15MetaboliteMetabolThor, PeterSuccinate
16MetaboliteMetabolThor, PeterMalate
17MetaboliteMetabolThor, Petera-ketoglutaric_acid
18MetaboliteMetabolThor, Peter8-Oxo-2-dexyguanosine
19MetaboliteMetabolThor, PeterAlanine
20MetaboliteMetabolThor, PeterAspartate
21MetaboliteMetabolThor, PeterBetaine
22MetaboliteMetabolThor, PeterCystine
23MetaboliteMetabolThor, PeterGlutamate
24MetaboliteMetabolThor, PeterMethionine
25MetaboliteMetabolThor, PeterPhospho-L-Arginine
26MetaboliteMetabolThor, PeterProline
27MetaboliteMetabolThor, PeterSarcosine
28MetaboliteMetabolThor, PeterSerine
29MetaboliteMetabolThor, PeterThreonine
30MetaboliteMetabolThor, PeterTyrosine
31MetaboliteMetabolThor, PeterValine
32MetaboliteMetabolThor, PeterAminobutyric acid
33MetaboliteMetabolThor, Petera-Aminoadipic_acid
34MetaboliteMetabolThor, PeterCaproic_acid
35MetaboliteMetabolThor, PeterCaprylic_acid
36MetaboliteMetabolThor, PeterCapric_acid
37MetaboliteMetabolThor, PeterUndecylic_acid
38MetaboliteMetabolThor, PeterLauric_acid
39MetaboliteMetabolThor, PeterTridecylic_acid
40MetaboliteMetabolThor, PeterMyristic_acid
41MetaboliteMetabolThor, PeterMyristoleic_acid
42MetaboliteMetabolThor, PeterPentadecylic_acid
43MetaboliteMetabolThor, PeterGinkgoic_acid
44MetaboliteMetabolThor, PeterPalmitic_acid
45MetaboliteMetabolThor, PeterPalmitoleic_acid
46MetaboliteMetabolThor, PeterElaidic_acid
47MetaboliteMetabolThor, PeterMargaric_acid
48MetaboliteMetabolThor, PeterStearic_acid
49MetaboliteMetabolThor, PeterLinolelaidic_acid
50MetaboliteMetabolThor, PeterArachidic_acid
51MetaboliteMetabolThor, PeterEicosenoic_acid
52MetaboliteMetabolThor, PeterEicosapentaenoic_acid
53MetaboliteMetabolThor, PeterErucic_acid
54MetaboliteMetabolThor, PeterDocosahexaenoic_acid
55MetaboliteMetabolThor, Petera-Glycerophosphate
56Temperature, waterTemp°CThor, Peter
57Temperature, water, standard deviationTemp std dev±Thor, Peter
58SalinitySalThor, Peter
59Salinity, standard deviationSal std dev±Thor, Peter
60pHpHThor, PeterTotal scale
61pH, standard deviationpH std dev±Thor, PeterTotal scale
62Alkalinity, totalATµmol/kgThor, Peter
63Alkalinity, total, standard deviationAT std dev±Thor, Peter
64Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmThor, Peter
65Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Thor, Peter
66Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
67Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
68Carbon dioxide, standard deviationCO2 std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
69Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
70Fugacity of carbon dioxide in seawater, standard deviationfCO2 std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
71Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
72Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
73Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
74Bicarbonate ion, standard deviation[HCO3]- std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
75Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
76Carbonate ion, standard deviation[CO3]2- std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
77Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
78Carbon, inorganic, dissolved, standard deviationDIC std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
79Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
80Aragonite saturation state, standard deviationOmega Arg std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
81Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
82Calcite saturation state, standard deviationOmega Cal std dev±Yang, YanCalculated using seacarb after Orr et al. (2018)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2720 data points

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