Thor, Peter; Vermandele, Fanny; Bailey, Allison; Guscelli, Ella; Loubet‑Sartrou, Léa; Dupont, Sam; Calosi, Piero (2022): Seawater carbonate chemistry and cellular metabolism of the Arctic copepod Calanus glacialis [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.957185
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Published: 2022 (exact date unknown) • DOI registered: 2023-05-02
Abstract:
Using a targeted metabolomic approach we investigated the effects of low seawater pH on energy metabolism in two late copepodite stages (CIV and CV) of the keystone Arctic copepod species Calanus glacialis. Exposure to decreasing seawater pH (from 8.0 to 7.0) caused increased ATP, ADP and NAD+ and decreased AMP concentrations in stage CIV, and increased ATP and phospho-L-arginine and decreased AMP concentrations in stage CV. Metabolic pathway enrichment analysis showed enrichment of the TCA cycle and a range of amino acid metabolic pathways in both stages. Concentrations of lactate, malate, fumarate and alpha-ketoglutarate (all involved in the TCA cycle) increased in stage CIV, whereas only alpha-ketoglutarate increased in stage CV. Based on the pattern of concentration changes in glucose, pyruvate, TCA cycle metabolites, and free amino acids, we hypothesise that ocean acidification will lead to a shift in energy production from carbohydrate metabolism in the glycolysis toward amino acid metabolism in the TCA cycle and oxidative phosphorylation in stage CIV. In stage CV, concentrations of most of the analysed free fatty acids increased, suggesting in particular that ocean acidification increases the metabolism of stored wax esters in this stage. Moreover, aminoacyl-tRNA biosynthesis was enriched in both stages indicating increased enzyme production to handle low pH stress.
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Supplement to:
Thor, Peter; Vermandele, Fanny; Bailey, Allison; Guscelli, Ella; Loubet‑Sartrou, Léa; Dupont, Sam; Calosi, Piero (2022): Ocean acidification causes fundamental changes in the cellular metabolism of the Arctic copepod Calanus glacialis as detected by metabolomic analysis. Scientific Reports, 12(1), 22223, https://doi.org/10.1038/s41598-022-26480-9
Documentation:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html
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Coverage:
Latitude: 79.000000 * Longitude: 11.700000
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Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-03.
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License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2720 data points
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Datasets with similar metadata
- Bailey, A; Thor, P; Browman, HI et al. (2017): Early life stages of the Arctic copepod Calanus glacialis are unaffected by increased seawater pCO2. https://doi.org/10.1594/PANGAEA.861734
- Thor, P; Bailey, A; Halsband, C et al. (2016): Seawater pH predicted for the year 2100 affects the metabolic response to feeding in Copepodites of the Arctic Copepod Calanus glacialis. https://doi.org/10.1594/PANGAEA.874790
- Wolf, KKE; Romanelli, E; Rost, B et al. (2019): Seawater carbonate chemistry and growth, production rates, and cellular composition of Arctic diatom. https://doi.org/10.1594/PANGAEA.913498
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