Citation:

PANGAEA.957185

Name: Seawater carbonate chemistry and cellular metabolism of the Arctic copepod Calanus glacialis
Published: 2022
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Arctic; Arthropoda; Bottles or small containers/Aquaria (<20 L); Calanus glacialis; Coast and continental shelf; Laboratory experiment; Other studied parameter or process; Pelagos; Polar; Single species; Zooplankton

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Abstract:

Using a targeted metabolomic approach we investigated the effects of low seawater pH on energy metabolism in two late copepodite stages (CIV and CV) of the keystone Arctic copepod species Calanus glacialis. Exposure to decreasing seawater pH (from 8.0 to 7.0) caused increased ATP, ADP and NAD+ and decreased AMP concentrations in stage CIV, and increased ATP and phospho-L-arginine and decreased AMP concentrations in stage CV. Metabolic pathway enrichment analysis showed enrichment of the TCA cycle and a range of amino acid metabolic pathways in both stages. Concentrations of lactate, malate, fumarate and alpha-ketoglutarate (all involved in the TCA cycle) increased in stage CIV, whereas only alpha-ketoglutarate increased in stage CV. Based on the pattern of concentration changes in glucose, pyruvate, TCA cycle metabolites, and free amino acids, we hypothesise that ocean acidification will lead to a shift in energy production from carbohydrate metabolism in the glycolysis toward amino acid metabolism in the TCA cycle and oxidative phosphorylation in stage CIV. In stage CV, concentrations of most of the analysed free fatty acids increased, suggesting in particular that ocean acidification increases the metabolism of stored wax esters in this stage. Moreover, aminoacyl-tRNA biosynthesis was enriched in both stages indicating increased enzyme production to handle low pH stress.

Keyword(s):

Animalia; Arctic; Arthropoda; Bottles or small containers/Aquaria (<20 L); Calanus glacialis; Coast and continental shelf; Laboratory experiment; Other studied parameter or process; Pelagos; Polar; Single species; Zooplankton

Supplement to:

Thor, Peter; Vermandele, Fanny; Bailey, Allison; Guscelli, Ella; Loubet‑Sartrou, Léa; Dupont, Sam; Calosi, Piero (2022): Ocean acidification causes fundamental changes in the cellular metabolism of the Arctic copepod Calanus glacialis as detected by metabolomic analysis. Scientific Reports, 12(1), 22223, https://doi.org/10.1038/s41598-022-26480-9

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 79.000000 * Longitude: 11.700000

Event(s):

Kongsfjord_copepod * Latitude: 79.000000 * Longitude: 11.700000 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-03.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Thor, Peter		Study
2	Species, unique identification	Species UID		Thor, Peter
3	Species, unique identification (URI)	Species UID (URI)		Thor, Peter
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Thor, Peter
5	Stage	Stage		Thor, Peter
6	pH	pH		Thor, Peter		Total scale
7	Metabolite	Metabol		Thor, Peter		ATP
8	Metabolite	Metabol		Thor, Peter		ADP
9	Metabolite	Metabol		Thor, Peter		AMP
10	Metabolite	Metabol		Thor, Peter		NAD, data in line 1 and 31 are outlier
11	Metabolite	Metabol		Thor, Peter		Glucose
12	Metabolite	Metabol		Thor, Peter		Lactate
13	Metabolite	Metabol		Thor, Peter		Pyruvate
14	Metabolite	Metabol		Thor, Peter		Fumarate
15	Metabolite	Metabol		Thor, Peter		Succinate
16	Metabolite	Metabol		Thor, Peter		Malate
17	Metabolite	Metabol		Thor, Peter		a-ketoglutaric_acid
18	Metabolite	Metabol		Thor, Peter		8-Oxo-2-dexyguanosine
19	Metabolite	Metabol		Thor, Peter		Alanine
20	Metabolite	Metabol		Thor, Peter		Aspartate
21	Metabolite	Metabol		Thor, Peter		Betaine
22	Metabolite	Metabol		Thor, Peter		Cystine
23	Metabolite	Metabol		Thor, Peter		Glutamate
24	Metabolite	Metabol		Thor, Peter		Methionine
25	Metabolite	Metabol		Thor, Peter		Phospho-L-Arginine
26	Metabolite	Metabol		Thor, Peter		Proline
27	Metabolite	Metabol		Thor, Peter		Sarcosine
28	Metabolite	Metabol		Thor, Peter		Serine
29	Metabolite	Metabol		Thor, Peter		Threonine
30	Metabolite	Metabol		Thor, Peter		Tyrosine
31	Metabolite	Metabol		Thor, Peter		Valine
32	Metabolite	Metabol		Thor, Peter		Aminobutyric acid
33	Metabolite	Metabol		Thor, Peter		a-Aminoadipic_acid
34	Metabolite	Metabol		Thor, Peter		Caproic_acid
35	Metabolite	Metabol		Thor, Peter		Caprylic_acid
36	Metabolite	Metabol		Thor, Peter		Capric_acid
37	Metabolite	Metabol		Thor, Peter		Undecylic_acid
38	Metabolite	Metabol		Thor, Peter		Lauric_acid
39	Metabolite	Metabol		Thor, Peter		Tridecylic_acid
40	Metabolite	Metabol		Thor, Peter		Myristic_acid
41	Metabolite	Metabol		Thor, Peter		Myristoleic_acid
42	Metabolite	Metabol		Thor, Peter		Pentadecylic_acid
43	Metabolite	Metabol		Thor, Peter		Ginkgoic_acid
44	Metabolite	Metabol		Thor, Peter		Palmitic_acid
45	Metabolite	Metabol		Thor, Peter		Palmitoleic_acid
46	Metabolite	Metabol		Thor, Peter		Elaidic_acid
47	Metabolite	Metabol		Thor, Peter		Margaric_acid
48	Metabolite	Metabol		Thor, Peter		Stearic_acid
49	Metabolite	Metabol		Thor, Peter		Linolelaidic_acid
50	Metabolite	Metabol		Thor, Peter		Arachidic_acid
51	Metabolite	Metabol		Thor, Peter		Eicosenoic_acid
52	Metabolite	Metabol		Thor, Peter		Eicosapentaenoic_acid
53	Metabolite	Metabol		Thor, Peter		Erucic_acid
54	Metabolite	Metabol		Thor, Peter		Docosahexaenoic_acid
55	Metabolite	Metabol		Thor, Peter		a-Glycerophosphate
56	Temperature, water	Temp	°C	Thor, Peter
57	Temperature, water, standard deviation	Temp std dev	±	Thor, Peter
58	Salinity	Sal		Thor, Peter
59	Salinity, standard deviation	Sal std dev	±	Thor, Peter
60	pH	pH		Thor, Peter		Total scale
61	pH, standard deviation	pH std dev	±	Thor, Peter		Total scale
62	Alkalinity, total	AT	µmol/kg	Thor, Peter
63	Alkalinity, total, standard deviation	AT std dev	±	Thor, Peter
64	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Thor, Peter
65	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Thor, Peter
66	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
67	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
68	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
69	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
70	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
71	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
72	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
73	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
74	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
75	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
76	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
77	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
78	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
79	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
80	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
81	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
82	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2720 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (Study)	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Stage	6 pH (Total scale)	7 Metabol (ATP)	8 Metabol (ADP)	9 Metabol (AMP)	10 Metabol (NAD, data in line 1 and 31 ar...)	11 Metabol (Glucose)	12 Metabol (Lactate)	13 Metabol (Pyruvate)	14 Metabol (Fumarate)	15 Metabol (Succinate)	16 Metabol (Malate)	17 Metabol (a-ketoglutaric_acid)	18 Metabol (8-Oxo-2-dexyguanosine)	19 Metabol (Alanine)	20 Metabol (Aspartate)	21 Metabol (Betaine)	22 Metabol (Cystine)	23 Metabol (Glutamate)	24 Metabol (Methionine)	25 Metabol (Phospho-L-Arginine)	26 Metabol (Proline)	27 Metabol (Sarcosine)	28 Metabol (Serine)	29 Metabol (Threonine)	30 Metabol (Tyrosine)	31 Metabol (Valine)	32 Metabol (Aminobutyric acid)	33 Metabol (a-Aminoadipic_acid)	34 Metabol (Caproic_acid)	35 Metabol (Caprylic_acid)	36 Metabol (Capric_acid)	37 Metabol (Undecylic_acid)	38 Metabol (Lauric_acid)	39 Metabol (Tridecylic_acid)	40 Metabol (Myristic_acid)	41 Metabol (Myristoleic_acid)	42 Metabol (Pentadecylic_acid)	43 Metabol (Ginkgoic_acid)	44 Metabol (Palmitic_acid)	45 Metabol (Palmitoleic_acid)	46 Metabol (Elaidic_acid)	47 Metabol (Margaric_acid)	48 Metabol (Stearic_acid)	49 Metabol (Linolelaidic_acid)	50 Metabol (Arachidic_acid)	51 Metabol (Eicosenoic_acid)	52 Metabol (Eicosapentaenoic_acid)	53 Metabol (Erucic_acid)	54 Metabol (Docosahexaenoic_acid)	55 Metabol (a-Glycerophosphate)	56 Temp [°C]	57 Temp std dev [±]	58 Sal	59 Sal std dev [±]	60 pH (Total scale)	61 pH std dev [±] (Total scale)	62 AT [µmol/kg]	63 AT std dev [±]	64 pCO2water_SST_wet [µatm]	65 pCO2 std dev [±]	66 CSC flag (Calculated using seacarb afte...)	67 CO2 [µmol/kg] (Calculated using seacarb afte...)	68 CO2 std dev [±] (Calculated using seacarb afte...)	69 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	70 fCO2 std dev [±] (Calculated using seacarb afte...)	71 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	72 pCO2 std dev [±] (Calculated using seacarb afte...)	73 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	74 [HCO3]- std dev [±] (Calculated using seacarb afte...)	75 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	76 [CO3]2- std dev [±] (Calculated using seacarb afte...)	77 DIC [µmol/kg] (Calculated using seacarb afte...)	78 DIC std dev [±] (Calculated using seacarb afte...)	79 Omega Arg (Calculated using seacarb afte...)	80 Omega Arg std dev [±] (Calculated using seacarb afte...)	81 Omega Cal (Calculated using seacarb afte...)	82 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	8.02	0.797	0.303	-0.542	2.274	-2.134	2.572	-1.212	-2.583	-2.416	-2.783	-1.27	-1.2	-1.311	1.166	-0.166	0.318	-0.474	-0.794	1.137	-0.406	-1.309	3.071	1.237	0.009	-0.415	-2.43	-1.476	1.718	-1.539	-0.876	-0.707	0.136	0.196	1.897	1.14	2.368	1.156	1.784	1.317	-0.293	2.901	1.235	0.115	0.444	-1.206	-1.826	-2.745	-1.53	-1.395	6.1	0.9	34	0.1	8.012	0.064	2347	11	450	95	8	22.23	3.71	440.78	72.51	442.58	72.81	2074.84	37.68	109.22	14.67	2206.29	27.51	1.65	0.22	2.62	0.35
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	8.02	-1.288	-0.94	1.007	-1.441	-1.059	0.964	0.57	-0.246	-0.235	-0.051	0.004	-0.463	-1.431	0.093	1.452	1.672	-0.132	1.36	-0.189	-0.805	-1.428	0.609	0.775	0.013	1.445	-0.571	-0.2	0.992	0.148	0.86	0.427	0.282	-0.871	1.002	-0.477	0.131	-0.61	0.795	-0.776	-0.214	-0.156	1.398	-1.633	0.644	-1.139	-1.154	-0.921	-0.614	0.099	6.1	0.9	34	0.1	8.012	0.064	2347	11	450	95	8	22.23	3.71	440.78	72.51	442.58	72.81	2074.84	37.68	109.22	14.67	2206.29	27.51	1.65	0.22	2.62	0.35
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.80	0.135	-0.186	0.574	-0.959	-0.869	1.108	-0.636	0.195	-0.069	-0.021	-0.091	0.541	0.864	1.195	0.077	-0.117	-1.035	-0.132	0.041	0.429	0.861	1.027	0.063	-0.551	0.015	1.375	-0.086	0.719	0.871	1.831	1.054	0.424	1.34	-0.1	0.657	0.344	0.744	-0.345	0.867	-1.53	0.284	-0.098	0.163	1.705	-1.263	0.39	-1.586	-0.054	0.422	6.0	0.7	34	0.1	7.851	0.062	2351	14	712	134	8	33.56	5.24	663.19	102.60	665.89	103.02	2156.43	28.99	78.04	10.43	2268.02	24.84	1.18	0.16	1.87	0.25
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.88	-1.312	-1.009	1.021	-1.376	-1.063	0.925	0.489	-0.057	0.456	0.07	0.205	0.831	-1.389	0.008	1.551	1.737	-1.961	0.872	-1.832	-0.987	-1.387	-0.731	-0.453	-0.54	1.508	-0.793	-0.429	0.633	1.549	0.168	1.617	-0.794	0.684	-0.179	-0.112	1.413	0.379	-0.291	0.348	-0.265	0.316	-0.315	-0.11	-0.291	-0.971	0.236	-0.047	0.405	-0.329	6.0	0.7	34	0.1	7.851	0.062	2351	14	712	134	8	33.56	5.24	663.19	102.60	665.89	103.02	2156.43	28.99	78.04	10.43	2268.02	24.84	1.18	0.16	1.87	0.25
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.88	-1.524	-1.407	1.093	-1.401	0.098	-0.409	1.947	-0.871	0.057	-0.411	-0.691	1.497	0.461	0.96	0.769	-0.642	-0.721	0.962	-1.709	-0.215	0.457	-0.226	0.187	0.008	0.717	0.778	0.047	1.377	0.506	0.965	0.695	1.46	1.526	1.409	-0.388	-0.537	-0.181	-1.163	-0.292	-0.815	-0.673	-0.878	0.56	-0.29	0.899	-0.006	0.92	1.343	0.264	6.0	0.7	34	0.1	7.851	0.062	2351	14	712	134	8	33.56	5.24	663.19	102.60	665.89	103.02	2156.43	28.99	78.04	10.43	2268.02	24.84	1.18	0.16	1.87	0.25
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.49	0.976	1.171	-1.381	0.538	1.169	-0.472	-0.93	-0.919	0.031	-0.777	-0.352	-0.763	0.871	-0.967	-0.123	-0.581	1.349	0.269	0.599	-1.398	0.871	-0.326	1.387	1.692	0.076	-0.696	-1.151	-0.115	0.634	-0.681	1.094	0.259	0.583	-0.816	-1.21	-0.468	-2.217	-0.37	-1.609	-0.183	-0.624	-0.188	-0.832	0.876	2.251	-0.501	1.109	-0.295	-0.816	6.3	0.8	34	0.1	7.442	0.088	2353	21	1973	460	8	89.93	18.98	1796.15	376.93	1803.45	378.46	2272.09	25.95	32.45	6.43	2394.47	35.75	0.49	0.10	0.78	0.15
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.50	0.089	-0.939	0.791	-0.372	-0.418	0.616	0.349	-0.095	-0.163	-0.276	-1.547	0.527	-0.25	-1.51	-0.312	-0.433	-0.973	-0.156	1.103	0.078	-0.25	-0.127	-0.604	-0.854	-0.325	0.714	0.711	-1.033	-0.298	-0.835	-0.092	0.669	-0.685	-0.395	0.051	0.201	-0.061	-0.048	0.019	0.641	0.199	0.356	-0.388	-1.527	-0.27	0.496	0.156	0.227	0.01	6.3	0.8	34	0.1	7.442	0.088	2353	21	1973	460	8	89.93	18.98	1796.15	376.93	1803.45	378.46	2272.09	25.95	32.45	6.43	2394.47	35.75	0.49	0.10	0.78	0.15
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.33	-0.044	0.576	0.262	0.146	0.582	-0.725	0.834	0.254	0.922	0.289	-0.451	1.197	-0.219	1.082	0.356	-0.159	0.991	0.237	-0.386	1.078	-0.221	-0.551	0.197	0.221	0.313	0.476	-0.093	-1.195	1.083	0.641	1.094	-0.791	0.607	0.137	-0.197	-0.69	0.06	0.021	-0.143	0.333	-0.719	0.123	-0.527	0.196	0.361	-0.294	0.554	-0.198	-0.542	6.4	0.7	34	0.1	7.318	0.067	2348	11	2414	308	8	120.08	19.17	2406.78	381.37	2416.56	382.92	2286.55	14.28	24.64	3.76	2431.28	27.29	0.37	0.06	0.59	0.09
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.33	-0.974	-1.396	1.065	0.154	0.127	-0.107	-0.021	0.623	0.319	0.465	-1.153	2.135	-0.503	0.975	-0.194	0.781	-0.351	0.194	-1.668	1.625	-0.504	0.201	-0.09	-0.322	-0.331	0.767	0.557	-0.796	-1.116	0.104	-0.268	-1.771	-1.704	-0.335	0.182	-0.186	0.285	-0.034	0.147	-0.665	0.818	0.185	0.499	-1.811	-0.589	1.384	-0.083	0.128	1.377	6.4	0.7	34	0.1	7.318	0.067	2348	11	2414	308	8	120.08	19.17	2406.78	381.37	2416.56	382.92	2286.55	14.28	24.64	3.76	2431.28	27.29	0.37	0.06	0.59	0.09
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.32	1.114	0.936	-1.608	0.423	1.501	-1.1	-0.803	-0.42	-0.828	-0.476	0.268	-0.753	-0.186	0.237	0.178	-0.051	1.829	0.943	0.806	-1.14	-0.186	0.533	1.318	1.758	0.158	-0.427	-0.851	-0.479	0.9	1.306	1.041	1.088	-1.183	-0.327	-2.128	-1.133	-0.86	-0.403	-0.997	-0.292	-0.74	0.81	-1.597	0.951	0.523	-0.188	0.483	-0.491	-1.203	6.4	0.7	34	0.1	7.318	0.067	2348	11	2414	308	8	120.08	19.17	2406.78	381.37	2416.56	382.92	2286.55	14.28	24.64	3.76	2431.28	27.29	0.37	0.06	0.59	0.09
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.16	0.644	0.012	0.035	0.259	0.368	-1.28	1.434	1.248	1.094	1.031	1.439	-1.397	1.219	-0.569	-1.091	-0.396	0.467	-1.48	0.804	0.226	1.219	-1.095	-0.15	-0.42	-1.031	0.854	0.82	0.515	-0.04	-0.622	-0.87	0.434	-1.546	-1.308	-0.208	-1.51	-0.82	-0.787	-0.471	-0.52	-1.585	-0.064	0.524	1.369	1.154	0.384	0.592	0.081	-0.016	6.3	0.8	34	0.1	7.160	0.063	2353	5	3546	543	8	175.03	26.16	3495.78	516.83	3510.01	518.93	2310.07	7.98	17.23	2.51	2502.34	30.38	0.26	0.04	0.41	0.06
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.16	0.685	0.878	-0.537	0.698	1.55	-1.16	-0.737	-0.944	-1.752	-1.148	-0.013	-0.256	2.262	-1.314	-1.516	-0.278	-0.301	-0.296	0.378	-1.196	2.268	0.306	0.292	1.582	-1.498	-1.098	-1.12	-0.165	-0.624	-0.396	0.349	-0.496	0.124	-1.04	-0.034	-0.702	-0.134	0.776	-0.082	0.603	0.184	1.005	0.456	0.431	-0.011	-0.884	0.471	-0.942	-0.404	6.3	0.8	34	0.1	7.160	0.063	2353	5	3546	543	8	175.03	26.16	3495.78	516.83	3510.01	518.93	2310.07	7.98	17.23	2.51	2502.34	30.38	0.26	0.04	0.41	0.06
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.06	0.851	1.341	-1.235	0.22	-0.767	0.044	-1.035	1.642	1.825	1.663	1.332	0.048	-0.099	1.568	-0.196	0.716	0.966	-0.434	1.03	-0.796	-0.1	-0.376	0.885	-1.009	-0.058	0.163	2.134	-1.259	-0.957	-1.067	-0.891	-1.597	-1.092	0.357	-1.249	-0.667	-1.054	1.683	-1.791	-1.556	0.249	1.065	-1.333	0.348	-0.989	-0.544	-0.879	-0.94	-0.027	6.4	0.7	34	0.1	6.998	0.044	2350	16	5132	526	8	254.59	26.65	5102.59	525.36	5123.38	527.51	2320.25	16.14	11.97	1.24	2586.81	33.48	0.18	0.02	0.29	0.03
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.06	1.122	0.896	-1.647	1.113	-0.44	0.406	-0.122	0.677	0.33	0.54	1.741	-0.671	-0.746	-0.825	-2.387	-2.684	0.381	-1.935	0.62	1.914	-0.744	-1.129	-2.277	-2.011	-2.426	1.225	1.508	0.601	1.549	1.467	-0.538	1.791	0.128	1.814	1.179	1.62	1.453	0.789	1.625	1.905	1.061	-0.231	1.631	-0.416	0.031	-1.011	0.516	-1.05	-0.937	6.4	0.7	34	0.1	6.998	0.044	2350	16	5132	526	8	254.59	26.65	5102.59	525.36	5123.38	527.51	2320.25	16.14	11.97	1.24	2586.81	33.48	0.18	0.02	0.29	0.03
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CIV	7.06	0.324	0.989	-0.24	0.666	0.52	-0.266	-1.066	1.034	0.461	0.99	1.339	-1.029	0.688	-0.324	0.114	-0.177	0.985	-0.854	0.064	0.663	0.687	-0.603	-0.162	-0.187	0.254	0.541	0.915	-0.694	-0.414	-0.695	-1.238	-0.866	0.785	-0.3	0.045	-0.208	0.271	0.135	-0.148	1.989	-0.108	-0.62	0.864	-0.433	0.938	0.247	0.576	0.107	-0.467	6.4	0.7	34	0.1	6.998	0.044	2350	16	5132	526	8	254.59	26.65	5102.59	525.36	5123.38	527.51	2320.25	16.14	11.97	1.24	2586.81	33.48	0.18	0.02	0.29	0.03
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	8.00	-1.908226915	-0.82633592	0.980220025	-1.71290714	0.577141431	0.079948361	-0.239252412	-1.29767676	0.380418269	-1.14603418	-1.22319627	0.419683627	2.167456595	0.224448964	-0.483999516	-0.579685821	-0.296621454	0.373869891	-1.795460987	-0.581751193	2.171565906	0.416838712	0.340837069	1.22590991	-0.528980147	0.67097288	-0.392261895	-0.058611917	-0.252004666	-0.244953098	0.018689636	-0.833964641	0.533920148	-0.306368894	-0.501107024	-0.692761436	-0.003931832	-0.744239682	-0.557823724	-1.266857299	-0.140899034	-0.65721251	-0.963561909	0.083601796	-0.924152614	1.330248107	-1.045691912	1.008690658	0.676718991	6.1	0.9	34	0.1	8.012	0.064	2347	11	450	95	8	22.23	3.71	440.78	72.51	442.58	72.81	2074.84	37.68	109.22	14.67	2206.29	27.51	1.65	0.22	2.62	0.35
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	8.01	-0.509803374	-0.069717833	0.833102847	-0.747942962	1.031763297	-0.387847382	-1.154004074	-1.351072521	-1.789053775	-1.365830928	-1.239375719	2.224858109	0.036291415	0.07886117	0.415739417	0.755798464	0.544818469	1.102078283	-0.173031201	-0.605932535	0.035855166	1.74643368	1.256776501	1.673642712	0.512433166	-0.006549049	-0.964132947	-0.645284942	-0.482350192	-0.619114148	-0.490205424	-0.586084874	-0.918977338	-0.026388158	0.589851598	-0.110718186	0.440731532	-0.131369315	0.821041912	-0.347924253	0.168461682	-0.062189444	0.114011654	-1.544101237	-0.391781543	0.409708244	-0.901948749	0.828026973	0.982020964	6.1	0.9	34	0.1	8.012	0.064	2347	11	450	95	8	22.23	3.71	440.78	72.51	442.58	72.81	2074.84	37.68	109.22	14.67	2206.29	27.51	1.65	0.22	2.62	0.35
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.81	0.036481184	0.78175611	0.455154974	0.799875088	0.07128777	-0.437081053	1.205797443	0.084047012	-0.27832594	0.075638774	1.152881657	0.109712804	0.347724629	1.481128947	0.308495684	0.174020814	2.36465935	-0.66931848	0.479887953	-0.494238957	0.345850354	0.320207163	-0.262994552	0.28318043	0.227490799	1.795170968	0.496726367	0.714812305	-1.168690697	0.536115898	1.839675705	-0.012794999	0.0742614	-0.205151718	-0.843724608	-0.209341654	-0.396638132	-0.13378732	-0.664897058	1.04094747	-1.309172668	0.246867354	1.894074856	0.824999449	1.236405128	-0.14910173	0.805172174	-0.640451016	0.095974615	6.0	0.7	34	0.1	7.851	0.062	2351	14	712	134	8	33.56	5.24	663.19	102.60	665.89	103.02	2156.43	28.99	78.04	10.43	2268.02	24.84	1.18	0.16	1.87	0.25
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.88	-0.587416054	-0.580326207	0.911316746	0.154701842	0.949857181	-0.421635237	-1.066654294	-1.215305477	0.33673309	-0.921635544	-1.540994255	-0.365191031	1.307114742	0.073046229	0.323249971	0.767070618	0.192024975	1.471989457	-1.010472692	-0.917406812	1.308027295	0.900397508	1.043428809	-1.674195808	0.452667969	-0.707650652	-0.909232271	-1.228823898	-0.904129004	-0.38721146	-1.360649974	-1.493227686	-0.743482275	-1.700995813	-1.730582885	-2.354316812	-1.95932577	-1.82180215	-2.452001877	-2.486778192	-2.358562514	-1.492627021	-1.357163698	-0.681704823	0.42932763	1.331943674	0.392248219	2.567097074	0.658052939	6.0	0.7	34	0.1	7.851	0.062	2351	14	712	134	8	33.56	5.24	663.19	102.60	665.89	103.02	2156.43	28.99	78.04	10.43	2268.02	24.84	1.18	0.16	1.87	0.25
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.88	-0.540838236	-0.388476523	0.880514967	0.168011618	-1.53499906	1.10109175	0.430045288	0.56931416	1.91077421	1.045719985	0.500750326	-1.046315917	-1.070466444	1.282908309	1.24689228	1.262728931	0.818019512	0.488594821	-0.55158363	1.108352681	-1.070571616	0.208923146	0.685566362	0.359973035	1.303746337	-0.674089788	1.307168578	0.197555194	-0.88733643	0.239269059	-0.618282051	0.552231926	-0.51515317	0.079154878	-0.180182966	0.414994739	0.066405805	-0.096193096	-0.233939571	0.163145528	-0.160185776	-0.0567724	-0.805544571	0.572950024	1.075571852	-0.277784576	1.329839813	-0.511567217	1.193323186	6.0	0.7	34	0.1	7.851	0.062	2351	14	712	134	8	33.56	5.24	663.19	102.60	665.89	103.02	2156.43	28.99	78.04	10.43	2268.02	24.84	1.18	0.16	1.87	0.25
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.86	-0.588890427	-0.026907327	0.807507551	0.574703133	-0.549651939	0.953575657	-0.449444224	0.720593753	0.087637905	0.925582887	-1.092575168	0.651190575	0.448948315	-0.004470316	-0.100862607	-0.176347417	-0.99614839	-0.482871491	-1.160529745	1.608431139	0.448392823	-0.083255823	-0.070332573	0.172471368	-0.217337983	0.891631179	0.541149651	0.603303679	0.585568417	1.428056871	0.355527787	-0.007148919	0.300897583	0.310241633	-0.377213982	-1.1274103	-0.252986595	-0.118980019	-0.515174444	-0.969029191	-0.795050752	-0.272621153	-1.212206599	-1.188350798	-0.599542455	1.168970427	-0.491987988	0.415938919	1.537440416	6.0	0.7	34	0.1	7.851	0.062	2351	14	712	134	8	33.56	5.24	663.19	102.60	665.89	103.02	2156.43	28.99	78.04	10.43	2268.02	24.84	1.18	0.16	1.87	0.25
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.86	-0.486152762	-0.544703414	0.907995314	-0.366658301	0.95033713	-0.619036537	-0.805436555	-1.138990752	1.018763878	-1.056573663	-1.169080256	-0.574322934	1.341753585	-2.199177989	0.112742971	0.99427012	0.344501867	1.062053068	-0.25774489	-0.789484367	1.34269911	1.029528319	1.602859077	-1.564492441	0.159471569	-0.605549869	-1.564205694	-2.154611525	0.298842256	-1.918245552	-1.430103206	-1.388446085	-1.136364643	-0.429143119	-0.222501269	0.369872738	0.256334269	-0.376015509	0.311399327	0.028553875	0.557403559	-0.52874551	0.822178101	0.23151471	0.09215659	0.298525647	-0.025471124	0.742276223	-0.404077209	6.0	0.7	34	0.1	7.851	0.062	2351	14	712	134	8	33.56	5.24	663.19	102.60	665.89	103.02	2156.43	28.99	78.04	10.43	2268.02	24.84	1.18	0.16	1.87	0.25
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.36	-0.994738569	-0.743290332	0.95580725	-0.549761396	1.224366519	-1.367007152	-1.219647792	-1.186911197	-1.662985974	-1.324898021	-1.10084741	2.079364133	0.414144109	0.049575493	0.315574723	1.495555398	0.94115246	2.168389054	-0.969767148	-0.501511218	0.413965085	1.120113306	2.274355733	-1.225756084	0.357950113	1.061045159	-2.066523574	-1.285335003	0.087876237	-1.948087065	-0.956887549	-0.835190002	-1.200695692	-1.134415674	-0.607686295	-0.667775893	-0.553368428	-0.761923238	-0.751118087	0.203432882	-0.655053937	-0.908054401	-0.212981621	-0.194552582	1.073338488	0.376624158	0.766086984	0.966378651	0.207422139	6.3	0.8	34	0.1	7.442	0.088	2353	21	1973	460	8	89.93	18.98	1796.15	376.93	1803.45	378.46	2272.09	25.95	32.45	6.43	2394.47	35.75	0.49	0.10	0.78	0.15
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.49	1.197640233	-1.124568675	-1.448815095	-1.149706552	0.742108764	-0.818933012	-1.130923184	0.510160156	1.392251051	0.492697921	0.927084884	0.073097671	-0.742280155	0.263749752	-0.360659376	0.213165681	0.178660241	0.444993294	1.10736891	-1.607746056	-0.741576044	-0.25580633	0.474025516	0.683355825	-0.217316464	-0.391852777	-0.32943395	0.819066187	-0.30581118	0.40447897	1.992591737	0.787732043	2.653117576	-1.244383481	-0.089091387	-0.585604247	-0.307891009	-1.211476424	-0.627584596	0.19631418	-0.601329093	-1.094684683	-0.665707438	0.005746984	0.503101724	1.645647107	0.07127669	0.757826606	-0.107423896	6.3	0.8	34	0.1	7.442	0.088	2353	21	1973	460	8	89.93	18.98	1796.15	376.93	1803.45	378.46	2272.09	25.95	32.45	6.43	2394.47	35.75	0.49	0.10	0.78	0.15
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.49	0.306650776	1.272994493	0.057780829	1.041265139	-0.071103935	0.786840459	0.45068342	-0.230858327	-1.106988208	-0.396176776	1.047785347	-1.351164378	0.049117785	1.734135626	0.131030653	-0.014051257	1.429917303	-0.951188045	0.777847346	-0.240181485	0.047997464	0.369106944	-0.701425713	0.48368216	0.159045926	-0.834775396	-0.169996514	-0.132483294	0.992461763	0.550412618	-0.927893104	-0.911109211	-0.829443592	-0.386657339	-1.014501148	0.691936276	-2.345566782	0.943363932	-0.339326338	0.26285311	0.034221758	0.559005799	0.351957906	0.958501204	1.555199624	-1.268537892	1.168887608	-1.268075114	-0.089146185	6.3	0.8	34	0.1	7.442	0.088	2353	21	1973	460	8	89.93	18.98	1796.15	376.93	1803.45	378.46	2272.09	25.95	32.45	6.43	2394.47	35.75	0.49	0.10	0.78	0.15
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.30	1.050342131	1.094830389	-1.251723829	0.814735802	-0.763795555	0.306647298	1.430540109	1.769625869	0.602829353	1.11986103	0.65057763	-0.587014518	0.086690111	0.186117783	-1.054428757	-1.016406969	0.253916262	-1.15448392	0.863048135	1.452761022	0.087846912	-0.113833073	-0.905116319	-0.454924263	-1.04366823	0.49409742	0.876121792	1.054578669	-0.195221844	-1.036504941	0.518618388	0.784139442	-0.230848407	0.832947681	0.254716687	0.33107941	0.530667966	1.15689547	0.337359762	-0.221552573	1.264693208	0.48641551	0.48885518	-1.098078318	-0.36486249	0.149176206	-1.163810181	-0.540911991	0.590433978	6.4	0.7	34	0.1	7.318	0.067	2348	11	2414	308	8	120.08	19.17	2406.78	381.37	2416.56	382.92	2286.55	14.28	24.64	3.76	2431.28	27.29	0.37	0.06	0.59	0.09
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.26	0.26347118	1.376915358	0.007800472	1.060733084	-1.578708093	1.500617296	-0.17055055	0.799715158	0.571607872	0.926859516	0.251090444	0.383890822	1.350411284	0.581154658	0.385707612	-0.617806944	0.282269794	-0.237119311	-0.057571446	0.750419623	1.348860168	0.262650784	-0.729144612	-0.312124736	0.352858675	2.378648302	0.868123922	-1.144397743	1.086095989	-0.041791963	-0.076835438	0.514000064	-0.647631926	0.463634557	0.578542652	0.814195162	0.783895172	1.075105438	0.938438498	0.750526694	0.938526636	0.568913231	0.020966859	0.393012323	0.18614785	-0.907567445	-0.231194051	-0.658207048	-1.182408875	6.4	0.7	34	0.1	7.318	0.067	2348	11	2414	308	8	120.08	19.17	2406.78	381.37	2416.56	382.92	2286.55	14.28	24.64	3.76	2431.28	27.29	0.37	0.06	0.59	0.09
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.32	1.168727677	-0.643394954	-1.375224332	1.501761432	0.801323699	-0.822241655	0.260867061	-0.674338477	-0.636499634	-0.912373505	-0.016997666	1.404417052	-0.838303021	-1.281683202	-2.225073023	-2.529096083	-1.242025199	-1.827081095	1.240794519	-1.207328713	-0.836497626	-2.275823536	-1.586939082	-0.220304822	-2.298491787	0.119323741	0.488128954	2.133457651	1.051988522	0.132359434	1.102043164	0.945822024	1.050099141	-0.238171914	0.333882136	1.401538095	0.438072935	0.130711636	0.581704373	0.721932831	0.656579055	0.723394326	0.9765391	1.252314853	-0.446911732	-0.933477894	1.081352559	-0.843720632	-1.124871181	6.4	0.7	34	0.1	7.318	0.067	2348	11	2414	308	8	120.08	19.17	2406.78	381.37	2416.56	382.92	2286.55	14.28	24.64	3.76	2431.28	27.29	0.37	0.06	0.59	0.09
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.15	0.62360174	1.483861148	-0.377808699	-0.005413697	-0.359857674	-0.666704493	2.410143941	-0.091094112	1.121765667	0.426132848	0.589052799	-0.237583116	-0.905736521	0.350762293	0.780760996	0.659648441	0.620850298	-0.093110126	0.753156477	1.28976171	-0.905906797	-0.685147591	-0.580931786	0.225041487	0.897881614	-1.467670669	-0.599306826	-0.285476351	0.921516759	1.553278803	1.186671343	0.350001114	-0.159378567	1.072576213	-0.476732418	-0.822237095	-0.551568682	-0.005906872	-0.352721884	0.074711499	-0.123312627	0.564147804	-0.286408256	1.550757628	0.198872786	-0.385919078	-0.084407299	-0.226783578	0.564018366	6.3	0.8	34	0.1	7.160	0.063	2353	5	3546	543	8	175.03	26.16	3495.78	516.83	3510.01	518.93	2310.07	7.98	17.23	2.51	2502.34	30.38	0.26	0.04	0.41	0.06
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.16	1.191138196	-1.004298569	-1.438322251	0.423531147	1.050813593	-1.269271549	-0.676544951	-0.180405729	-0.903129917	-0.341094735	0.200337729	-0.886392343	-1.019517875	-1.62156803	-1.858300558	-0.990179687	-1.179737348	-0.030361209	1.224124977	-1.086722161	-1.01822058	-1.730864777	0.187976386	1.078663773	-1.918135736	-1.056772176	0.563557443	0.634719857	-1.05573883	0.985893976	0.189211217	0.64065347	-0.001501425	0.949757319	-0.284054263	0.405723895	-0.16030909	2.080887861	-0.352669187	-0.533459208	1.039773465	2.321043907	-1.780344641	-1.179561728	-1.330162779	-1.206582328	-0.840842237	-1.315197526	-1.349342843	6.3	0.8	34	0.1	7.160	0.063	2353	5	3546	543	8	175.03	26.16	3495.78	516.83	3510.01	518.93	2310.07	7.98	17.23	2.51	2502.34	30.38	0.26	0.04	0.41	0.06
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	6.99	1.206305698	-2.019783743	-1.450746905	-2.00431786	1.197479808	-1.599010901	-0.834164714	-0.831567964	-0.609186936	-1.192817025	-0.577474993	-0.227279135	-1.338385183	-1.666629473	-1.578024881	-0.316407431	-1.084063861	-0.78042246	1.264523874	-1.017158348	-1.336878405	-0.848067906	0.058752315	-2.296983519	-1.456210426	-0.550743875	-1.408082117	-0.887886014	-1.558528097	-1.414302181	-1.354797416	-1.493977901	-1.302874779	-1.927037487	-0.983900889	-1.05989716	-0.849101595	-1.469798267	-0.702400058	0.229924613	-1.572652187	-1.127775147	0.577438333	2.013402598	2.306566979	-1.003341114	2.308903576	-0.835579777	-0.911747737	6.4	0.7	34	0.1	6.998	0.044	2350	16	5132	526	8	254.59	26.65	5102.59	525.36	5123.38	527.51	2320.25	16.14	11.97	1.24	2586.81	33.48	0.18	0.02	0.29	0.03
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.00	1.12712729	0.426171432	-1.338653151	0.979347147	-0.582123269	0.217189262	1.021472226	1.768316092	0.48891556	1.228601081	1.267650532	-0.13666705	-0.819974883	-0.48609259	-0.20848058	-0.917531088	-1.525979405	-0.547144479	1.146711619	0.525932071	-0.819890259	-1.300291292	-0.775390337	-0.020648198	-0.252523728	0.071408349	2.027448807	0.73538121	1.004421191	0.096564521	-0.083838739	0.723145236	0.256494779	1.458730384	0.421286169	0.775909106	0.831335459	1.436031492	0.437312155	-0.936975129	1.416046153	1.966959123	-0.933412605	-1.119824985	-1.123558366	-1.293503629	0.291869416	-1.073255106	0.617880608	6.4	0.7	34	0.1	6.998	0.044	2350	16	5132	526	8	254.59	26.65	5102.59	525.36	5123.38	527.51	2320.25	16.14	11.97	1.24	2586.81	33.48	0.18	0.02	0.29	0.03
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	6.99	-0.714468358	0.109046003	0.800296231	-0.425805488	-1.480685949	1.830367869	-0.62751065	0.09655243	-0.687377688	0.359809291	1.052674115	-0.252544349	-1.293364417	-0.027043069	1.616711265	0.922488294	-1.492585559	-0.448986849	-1.571887371	0.054956486	-1.292702533	-0.722634412	-1.389221452	0.009041915	1.483956825	-1.14753696	0.460926438	0.36680773	1.70076523	1.180269664	0.844017342	2.160551773	1.004681494	1.657664537	2.915857742	2.125180367	2.171708014	-0.460717102	2.524323057	2.709481307	0.918944295	-0.84967137	2.055618487	-0.986914659	-0.648193926	-0.270458829	-0.262119126	0.061254683	-2.466538831	6.4	0.7	34	0.1	6.998	0.044	2350	16	5132	526	8	254.59	26.65	5102.59	525.36	5123.38	527.51	2320.25	16.14	11.97	1.24	2586.81	33.48	0.18	0.02	0.29	0.03
laboratory	Calanus glacialis	marinespecies.org	urn:lsid:marinespecies.org:taxname:104465	CV	7.00	0.56786621	1.780767656	-0.61290572	1.137537215	-1.433768014	1.522851079	-0.071839657	1.111399688	-0.989860675	1.24113244	1.033141549	-1.579903584	0.919104303	0.099691644	0.681497011	-0.724954056	0.014975943	-1.320288595	0.193128624	0.501487542	0.915556946	0.028260104	-0.648485968	0.197670049	0.559892257	0.943514373	0.431454813	1.038638174	1.383837963	-0.632214633	0.342146812	1.194808924	1.482211495	1.340788134	1.866915908	0.998418464	1.458345034	1.203893041	1.553131942	0.391113937	1.198757996	0.957634681	0.234791309	0.588837403	-0.830677719	-1.267766551	-0.174141676	-0.944335891	-0.816150576	6.4	0.7	34	0.1	6.998	0.044	2350	16	5132	526	8	254.59	26.65	5102.59	525.36	5123.38	527.51	2320.25	16.14	11.97	1.24	2586.81	33.48	0.18	0.02	0.29	0.03