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Hoins, Mirja; Eberlein, Tim; Großmann, Christian H; Brandenburg, Karen; Reichart, Gert-Jan; Rost, Björn; Sluijs, Appy; Van de Waal, Dedmer B (2016): Combined effects of ocean acidification and light or nitrogen availabilities on 13C fractionation in marine dinoflagellates. PANGAEA, https://doi.org/10.1594/PANGAEA.861820, Supplement to: Hoins, M et al. (2016): Combined effects of ocean acidification and light or nitrogen availabilities on 13C fractionation in marine dinoflagellates. PLoS ONE, 11(5), e0154370, https://doi.org/10.1371/journal.pone.0154370

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Abstract:
Along with increasing oceanic CO2 concentrations, enhanced stratification constrains phytoplankton to shallower upper mixed layers with altered light regimes and nutrient concentrations. Here, we investigate the effects of elevated pCO2 in combination with light or nitrogen-limitation on 13C fractionation (epsilon p) in four dinoflagellate species. We cultured Gonyaulax spinifera and Protoceratium reticulatum in dilute batches under low-light (LL) and high-light (HL) conditions, and grew Alexandrium fundyense and Scrippsiella trochoidea in nitrogen-limited continuous cultures (LN) and nitrogen-replete batches (HN). The observed CO2-dependency of epsilon p remained unaffected by the availability of light for both G. spinifera and P. reticulatum, though at HL epsilon p was consistently lower by about 2.7 per mil over the tested CO2 range for P. reticulatum. This may reflect increased uptake of (13C-enriched) bicarbonate fueled by increased ATP production under HL conditions. The observed CO2-dependency of epsilon p disappeared under LN conditions in both A. fundyense and S. trochoidea. The generally higher epsilon p under LN may be associated with lower organic carbon production rates and/or higher ATP:NADPH ratios. CO2-dependent epsilon p under non-limiting conditions has been observed in several dinoflagellate species, showing potential for a new CO2-proxy. Our results however demonstrate that light- and nitrogen-limitation also affect epsilon p, thereby illustrating the need to carefully consider prevailing environmental conditions.
Keyword(s):
Alexandrium fundyense; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria ( 20 L); Chromista; Gonyaulax spinifera; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; Myzozoa; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Protoceratium reticulatum; Scrippsiella trochoidea; Single species
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloise (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-06-15.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1TypeTypeHoins, Mirjastudy
2SpeciesSpeciesHoins, Mirja
3Registration number of speciesReg spec noHoins, Mirja
4Uniform resource locator/link to referenceURL refHoins, MirjaWoRMS Aphia ID
5TreatmentTreatHoins, Mirja
6Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmHoins, Mirjatreatment
7Growth rateµ1/dayHoins, Mirja
8Growth rate, standard deviationµ std dev±Hoins, Mirja
9Carbon, organic, particulate, per cellPOCpg/#Hoins, Mirja
10Carbon, organic, particulate, standard deviationPOC std dev±Hoins, Mirja
11Chlorophyll a per cellChl apg/#Hoins, Mirja
12Chlorophyll a, standard deviationChl a std dev±Hoins, Mirja
13Isotopic fractionation, during photosynthisepsilon pHoins, Mirja13C fractionation
14Isotopic fractionation, during photosynthis, standard deviationepsilon p std e±Hoins, Mirja13C fractionation
15Carbon, organic, particulate/Nitrogen, organic, particulate ratioPOC/PONHoins, Mirja
16Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviationPOC/PON std dev±Hoins, Mirja
17Temperature, waterTemp°CHoins, Mirja
18SalinitySalHoins, Mirja
19Carbon dioxideCO2µmol/lHoins, MirjaCalculated using CO2SYS
20Carbon dioxide, standard deviationCO2 std dev±Hoins, MirjaCalculated using CO2SYS
21Alkalinity, totalATµmol/lHoins, MirjaPotentiometric titration
22Alkalinity, total, standard deviationAT std dev±Hoins, MirjaPotentiometric titration
23Carbon, inorganic, dissolvedDICµmol/lHoins, MirjaCalculated using CO2SYS
24Carbon, inorganic, dissolved, standard deviationDIC std dev±Hoins, MirjaCalculated using CO2SYS
25pHpHHoins, MirjaPotentiometricNBS scale
26pH, standard deviationpH std dev±Hoins, MirjaPotentiometricNBS scale
27Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
29Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
1008 data points

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