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Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Bjoern (2010): Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010. PANGAEA,, Supplement to: Kranz, SA et al. (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345,

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Recent studies on the diazotrophic cyanobacterium Trichodesmium erythraeum(IMS101) showed that increasing CO2 partial pressure (pCO2) enhances N2 fixation and growth. Significant uncertainties remain as to the degree of the sensitivity to pCO2, its modification by other environmental factors, and underlying processes causing these responses. To address these questions, we examined the responses ofTrichodesmium IMS101 grown under a matrix of low and high levels of pCO2 (150 and 900 µatm) and irradiance (50 and 200 µmol photons m-2 s-1). Growth rates as well as cellular carbon and nitrogen contents increased with increasing pCO2 and light levels in the cultures. The pCO2-dependent stimulation in organic carbon and nitrogen production was highest under low light. High pCO2 stimulated rates of N2fixation and prolonged the duration, while high light affected maximum rates only. Gross photosynthesis increased with light but did not change with pCO2. HCO3- was identified as the predominant carbon source taken up in all treatments. Inorganic carbon uptake increased with light, but only gross CO2 uptake was enhanced under high pCO2. A comparison between carbon fluxes in vivo and those derived from 13C fractionation indicates high internal carbon cycling, especially in the low-pCO2treatment under high light. Light-dependent oxygen uptake was only detected underlow pCO2 combined with high light or when low-light-acclimated cells were exposed to high light, indicating that the Mehler reaction functions also as a photoprotective mechanism in Trichodesmium. Our data confirm the pronounced pCO2 effect on N2fixation and growth in Trichodesmium and further show a strong modulation of these effects by light intensity. We attribute these responses to changes in the allocation of photosynthetic energy between carbon acquisition and the assimilation of carbon and nitrogen under elevated pCO2. These findings are supported by a complementarystudy looking at photosynthetic fluorescence parameters of photosystem II, photosynthetic unit stoichiometry (photosystem I:photosystem II), and pool sizes of key proteins in carbon and nitrogen acquisition.
Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Trichodesmium sp.
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1CommentCommentKranz, Sven A
2SalinitySalKranz, Sven A
3Temperature, waterTemp°CKranz, Sven A
4Radiation, photosynthetically activePARµmol/m2/sKranz, Sven A
5Experimental treatmentExp treatKranz, Sven A
6Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmKranz, Sven ACalculated using CO2SYS
7Carbon dioxideCO2µmol/kgKranz, Sven A
8Carbon dioxide, standard deviationCO2 std dev±Kranz, Sven ACalculated using CO2SYS
9Alkalinity, totalATµmol/kgKranz, Sven AAlkalinity, Gran titration (Gran, 1950)
10Alkalinity, total, standard deviationAT std dev±Kranz, Sven A
11pHpHKranz, Sven ApH meter (Metrohm electrodes)NBS scale
12pH, standard deviationpH std dev±Kranz, Sven A
13Carbon, inorganic, dissolvedDICµmol/kgKranz, Sven ACalculated using CO2SYS
14Carbon, inorganic, dissolved, standard deviationDIC std dev±Kranz, Sven A
15Growth rateµ#/dayKranz, Sven ACalculated, see reference(s)
16Growth rate, standard deviationµ std dev±Kranz, Sven A
17Carbon, organic, particulate, per cellPOCpg/#Kranz, Sven AMass spectrometer ANCA-SL 20-20 Europa Scientific
18Particulate organic carbon content per cell, standard deviationPOC cont std dev±Kranz, Sven A
19Particulate organic nitrogen per cellPON cellpmol/#Kranz, Sven AMass spectrometer ANCA-SL 20-20 Europa Scientific
20Particulate organic nitrogen per cell, standard deviationPON cell std dev±Kranz, Sven A
21Particulate organic phosphorus per cellPOP cellpmol/#Kranz, Sven AMass spectrometer ANCA-SL 20-20 Europa Scientific
22Particulate organic phosphorus per cell, standard deviationPOP cell std dev±Kranz, Sven A
23Carbon/Nitrogen ratioC/NKranz, Sven ACalculated
24Carbon/Nitrogen ratio, standard deviationC/N std dev±Kranz, Sven A
25Chlorophyll a per cellChl apg/#Kranz, Sven A
26Chlorophyll a, standard deviationChl a std dev±Kranz, Sven A
27Production of particulate organic carbon per cellPOC prodµmol/#/dayKranz, Sven ACalculated, see reference(s)
28Particulate organic carbon, production, standard deviationPOC prod std dev±Kranz, Sven A
29Production of particulate organic nitrogenPON prodpg/#/dayKranz, Sven ACalculated, see reference(s)
30Particulate organic nitrogen production, standard deviationPON prod std dev±Kranz, Sven A
31Gross oxygen evolution, per chlorophyll aO2 ev/Chlµmol/mg/hKranz, Sven Asee reference(s)
32Gross oxygen evolution, standard deviationO2 ev/Chl std dev±Kranz, Sven A
33Oxygen consumption per chlorophyll aO2 con/Chlµmol/mg/hKranz, Sven Asee reference(s)
34Oxygen consumption, standard deviationO2 con std dev±Kranz, Sven A
35Fixation of carbon in chlorophyllC/Chl fixmg/mg/hKranz, Sven A
36Fixation of carbon in chlorophyll, standard deviationC/Chl std dev±Kranz, Sven A
37Bicarbonate uptake rate in chlorophyllHCO3 upt rate/Chlµmol/mg/hKranz, Sven A
38Bicarbonate uptake in chlorophyll, standard deviationHCO3 upt/Chl std dev±Kranz, Sven A
39Carbon dioxide uptake rate in chlorophyllCO2 upt rate/Chlµmol/mg/hKranz, Sven A
40Carbon dioxide uptake in chlorophyll, standard deviationCO2 upt/Chl std dev±Kranz, Sven A
41Time in hoursTimehKranz, Sven A
42Nitrogen fixation rate per chlorophyll aN2 fix/Chlnmol/µg/hKranz, Sven ADetermined by acetylene reduction assay using a gas chromatograph
43Nitrogen fixation rate, standard deviationN2 fix std dev±Kranz, Sven A
44Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
45pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
46Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
47Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
48Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
49Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
50Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
51Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
52Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
53Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
1788 data points

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