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Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Björn (2010): Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010. PANGAEA, https://doi.org/10.1594/PANGAEA.777430, Supplement to: Kranz, SA et al. (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345, https://doi.org/10.1104/pp.110.159145

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Abstract:
Recent studies on the diazotrophic cyanobacterium Trichodesmium erythraeum(IMS101) showed that increasing CO2 partial pressure (pCO2) enhances N2 fixation and growth. Significant uncertainties remain as to the degree of the sensitivity to pCO2, its modification by other environmental factors, and underlying processes causing these responses. To address these questions, we examined the responses ofTrichodesmium IMS101 grown under a matrix of low and high levels of pCO2 (150 and 900 µatm) and irradiance (50 and 200 µmol photons m-2 s-1). Growth rates as well as cellular carbon and nitrogen contents increased with increasing pCO2 and light levels in the cultures. The pCO2-dependent stimulation in organic carbon and nitrogen production was highest under low light. High pCO2 stimulated rates of N2fixation and prolonged the duration, while high light affected maximum rates only. Gross photosynthesis increased with light but did not change with pCO2. HCO3- was identified as the predominant carbon source taken up in all treatments. Inorganic carbon uptake increased with light, but only gross CO2 uptake was enhanced under high pCO2. A comparison between carbon fluxes in vivo and those derived from 13C fractionation indicates high internal carbon cycling, especially in the low-pCO2treatment under high light. Light-dependent oxygen uptake was only detected underlow pCO2 combined with high light or when low-light-acclimated cells were exposed to high light, indicating that the Mehler reaction functions also as a photoprotective mechanism in Trichodesmium. Our data confirm the pronounced pCO2 effect on N2fixation and growth in Trichodesmium and further show a strong modulation of these effects by light intensity. We attribute these responses to changes in the allocation of photosynthetic energy between carbon acquisition and the assimilation of carbon and nitrogen under elevated pCO2. These findings are supported by a complementarystudy looking at photosynthetic fluorescence parameters of photosystem II, photosynthetic unit stoichiometry (photosystem I:photosystem II), and pool sizes of key proteins in carbon and nitrogen acquisition.
Keyword(s):
Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Trichodesmium sp.
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
CommentCommentKranz, Sven A
SalinitySalKranz, Sven A
Temperature, waterTemp°CKranz, Sven A
Radiation, photosynthetically activePARµmol/m2/sKranz, Sven A
Experimental treatmentExp treatKranz, Sven A
Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmKranz, Sven ACalculated using CO2SYS
Carbon dioxideCO2µmol/kgKranz, Sven A
Carbon dioxide, standard deviationCO2 std dev±Kranz, Sven ACalculated using CO2SYS
Alkalinity, totalATµmol/kgKranz, Sven AAlkalinity, Gran titration (Gran, 1950)
10 Alkalinity, total, standard deviationAT std dev±Kranz, Sven A
11 pHpHKranz, Sven ApH meter (Metrohm electrodes)NBS scale
12 pH, standard deviationpH std dev±Kranz, Sven A
13 Carbon, inorganic, dissolvedDICµmol/kgKranz, Sven ACalculated using CO2SYS
14 Carbon, inorganic, dissolved, standard deviationDIC std dev±Kranz, Sven A
15 Growth rateµ#/dayKranz, Sven ACalculated, see reference(s)
16 Growth rate, standard deviationµ std dev±Kranz, Sven A
17 Carbon, organic, particulate, per cellPOC/cellpg/#Kranz, Sven AMass spectrometer ANCA-SL 20-20 Europa Scientific
18 Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Kranz, Sven A
19 Particulate organic nitrogen per cellPON/cellpmol/#Kranz, Sven AMass spectrometer ANCA-SL 20-20 Europa Scientific
20 Particulate organic nitrogen per cell, standard deviationPON/cell std dev±Kranz, Sven A
21 Particulate organic phosphorus per cellPOP/cellpmol/#Kranz, Sven AMass spectrometer ANCA-SL 20-20 Europa Scientific
22 Particulate organic phosphorus per cell, standard deviationPOP/cell std dev±Kranz, Sven A
23 Carbon/Nitrogen ratioC/NKranz, Sven ACalculated
24 Carbon/Nitrogen ratio, standard deviationC/N std dev±Kranz, Sven A
25 Chlorophyll a per cellChl a/cellpg/#Kranz, Sven A
26 Chlorophyll a, standard deviationChl a std dev±Kranz, Sven A
27 Particulate organic carbon production per cellPOC prod/cellµmol/#/dayKranz, Sven ACalculated, see reference(s)
28 Particulate organic carbon, production, standard deviationPOC prod std dev±Kranz, Sven A
29 Production of particulate organic nitrogenPON prodpg/#/dayKranz, Sven ACalculated, see reference(s)
30 Particulate organic nitrogen production, standard deviationPON prod std dev±Kranz, Sven A
31 Gross oxygen evolution, per chlorophyll aO2 ev/Chlµmol/mg/hKranz, Sven Asee reference(s)
32 Gross oxygen evolution, standard deviationO2 ev/Chl std dev±Kranz, Sven A
33 Oxygen consumption per chlorophyll aO2 con/Chlµmol/mg/hKranz, Sven Asee reference(s)
34 Oxygen consumption, standard deviationO2 con std dev±Kranz, Sven A
35 Fixation of carbon in chlorophyllC/Chl fixmg/mg/hKranz, Sven A
36 Fixation of carbon in chlorophyll, standard deviationC/Chl std dev±Kranz, Sven A
37 Bicarbonate uptake rate, per chlorophyll aHCO3 upt rate/Chlµmol/mg/hKranz, Sven A
38 Bicarbonate uptake in chlorophyll, standard deviationHCO3 upt/Chl std dev±Kranz, Sven A
39 Carbon dioxide uptake rate, per chlorophyll aCO2 upt rate/Chlµmol/mg/hKranz, Sven A
40 Carbon dioxide uptake, per chlorophyll, standard deviationCO2 upt/Chl std dev±Kranz, Sven A
41 Time in hoursTimehKranz, Sven A
42 Nitrogen fixation rate per chlorophyll aN2 fix/Chlnmol/µg/hKranz, Sven ADetermined by acetylene reduction assay using a gas chromatograph
43 Nitrogen fixation rate, standard deviationN2 fix std dev±Kranz, Sven A
44 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
45 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
46 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
47 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
48 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
49 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
50 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
51 Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
52 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
53 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1788 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Comment

Sal

Temp [°C]

PAR [µmol/m2/s]

Exp treat

pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)

CO2 [µmol/kg]

CO2 std dev [±]
(Calculated using CO2SYS)

AT [µmol/kg]
(Alkalinity, Gran titration (G...)
10 
AT std dev [±]
11 
pH
(NBS scale, pH meter (Metrohm ...)
12 
pH std dev [±]
13 
DIC [µmol/kg]
(Calculated using CO2SYS)
14 
DIC std dev [±]
15 
µ [#/day]
(Calculated, see reference(s))
16 
µ std dev [±]
17 
POC/cell [pg/#]
(Mass spectrometer ANCA-SL 20-...)
18 
POC cont/cell std dev [±]
19 
PON/cell [pmol/#]
(Mass spectrometer ANCA-SL 20-...)
20 
PON/cell std dev [±]
21 
POP/cell [pmol/#]
(Mass spectrometer ANCA-SL 20-...)
22 
POP/cell std dev [±]
23 
C/N
(Calculated)
24 
C/N std dev [±]
25 
Chl a/cell [pg/#]
26 
Chl a std dev [±]
27 
POC prod/cell [µmol/#/day]
(Calculated, see reference(s))
28 
POC prod std dev [±]
29 
PON prod [pg/#/day]
(Calculated, see reference(s))
30 
PON prod std dev [±]
31 
O2 ev/Chl [µmol/mg/h]
(see reference(s))
32 
O2 ev/Chl std dev [±]
33 
O2 con/Chl [µmol/mg/h]
(see reference(s))
34 
O2 con std dev [±]
35 
C/Chl fix [mg/mg/h]
36 
C/Chl std dev [±]
37 
HCO3 upt rate/Chl [µmol/mg/h]
38 
HCO3 upt/Chl std dev [±]
39 
CO2 upt rate/Chl [µmol/mg/h]
40 
CO2 upt/Chl std dev [±]
41 
Time [h]
42 
N2 fix/Chl [nmol/µg/h]
(Determined by acetylene reduc...)
43 
N2 fix std dev [±]
44 
CSC flag
(Calculated using seacarb afte...)
45 
pH
(Total scale, Calculated using...)
46 
CO2 [µmol/kg]
(Calculated using seacarb afte...)
47 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
48 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
49 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)
50 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
51 
DIC [µmol/kg]
(Calculated using seacarb afte...)
52 
Omega Arg
(Calculated using seacarb afte...)
53 
Omega Cal
(Calculated using seacarb afte...)
acclimation light362550150 µatm CO21503.80.3248798.570.031841190.150.030.3160.0080.590.037300090006.410.390.470.045700001100000.0064290.001429268.433.68130.57130.161432.60431.021867.306.7910.28
acclimation light362550900 µatm CO290023.31.52470147.940.032240180.240.030.3760.0180.880.067800090005.040.150.720.0510900001700000.0150000.002857267.8023.08819.70817.122108.58148.742280.402.343.55
acclimation light3625200150 µatm CO21503.80.3248798.570.031841190.380.020.3830.0380.860.0870000140005.250.190.670.1417600002600000.0235710.003571268.433.68130.57130.161432.60431.021867.306.7910.28
acclimation light3625200900 µatm CO290023.31.52470147.940.032240180.420.020.4180.0481.040.097100040004.850.100.690.0821200003400000.0314290.004286267.8023.08819.70817.122108.58148.742280.402.343.55
low light362550150 µatm CO21503.80.3248798.570.03184119143168121268.433.68130.57130.161432.60431.021867.306.7910.28
low light362550150 µatm CO21503.80.3248798.570.0318411915642712268.433.68130.57130.161432.60431.021867.306.7910.28
high light362550150 µatm CO21503.80.3248798.570.03184119453200268.433.68130.57130.161432.60431.021867.306.7910.28
high light362550150 µatm CO21503.80.3248798.570.0318411961281268.433.68130.57130.161432.60431.021867.306.7910.28
dark362550150 µatm CO21503.80.3248798.570.0318411911510268.433.68130.57130.161432.60431.021867.306.7910.28
dark362550150 µatm CO21503.80.3248798.570.03184119258268.433.68130.57130.161432.60431.021867.306.7910.28
low light362550900 µatm CO290023.31.52470147.940.03224018119224618267.8023.08819.70817.122108.58148.742280.402.343.55
low light362550900 µatm CO290023.31.52470147.940.03224018135172323267.8023.08819.70817.122108.58148.742280.402.343.55
high light362550900 µatm CO290023.31.52470147.940.032240185387013733267.8023.08819.70817.122108.58148.742280.402.343.55
high light362550900 µatm CO290023.31.52470147.940.03224018429428362267.8023.08819.70817.122108.58148.742280.402.343.55
dark362550900 µatm CO290023.31.52470147.940.032240188311267.8023.08819.70817.122108.58148.742280.402.343.55
dark362550900 µatm CO290023.31.52470147.940.03224018247267.8023.08819.70817.122108.58148.742280.402.343.55
low light3625200150 µatm CO21503.80.3248798.570.03184119268.433.68130.57130.161432.60431.021867.306.7910.28
low light3625200150 µatm CO21503.80.3248798.570.03184119268.433.68130.57130.161432.60431.021867.306.7910.28
high light3625200150 µatm CO21503.80.3248798.570.031841194542825449268.433.68130.57130.161432.60431.021867.306.7910.28
high light3625200150 µatm CO21503.80.3248798.570.031841194868111557268.433.68130.57130.161432.60431.021867.306.7910.28
dark3625200150 µatm CO21503.80.3248798.570.0318411910644268.433.68130.57130.161432.60431.021867.306.7910.28
dark3625200150 µatm CO21503.80.3248798.570.031841196713268.433.68130.57130.161432.60431.021867.306.7910.28
low light3625200900 µatm CO290023.31.52470147.940.03224018267.8023.08819.70817.122108.58148.742280.402.343.55
low light3625200900 µatm CO290023.31.52470147.940.03224018267.8023.08819.70817.122108.58148.742280.402.343.55
high light3625200900 µatm CO290023.31.52470147.940.032240185345111742267.8023.08819.70817.122108.58148.742280.402.343.55
high light3625200900 µatm CO290023.31.52470147.940.0322401843215312327267.8023.08819.70817.122108.58148.742280.402.343.55
dark3625200900 µatm CO290023.31.52470147.940.0322401812630267.8023.08819.70817.122108.58148.742280.402.343.55
dark3625200900 µatm CO290023.31.52470147.940.0322401811124267.8023.08819.70817.122108.58148.742280.402.343.55
acclimation light362550150 µatm CO21503.80.3248798.570.031841199841058101268.433.68130.57130.161432.60431.021867.306.7910.28
acclimation light362550150 µatm CO21503.80.3248798.570.03184119921012125175268.433.68130.57130.161432.60431.021867.306.7910.28
acclimation light362550900 µatm CO290023.31.52470147.940.0322401869782192210267.8023.08819.70817.122108.58148.742280.402.343.55
acclimation light362550900 µatm CO290023.31.52470147.940.03224018528988196267.8023.08819.70817.122108.58148.742280.402.343.55
acclimation light3625200150 µatm CO21503.80.3248798.570.03184119301924750596268.433.68130.57130.161432.60431.021867.306.7910.28
acclimation light3625200150 µatm CO21503.80.3248798.570.031841193304028750618268.433.68130.57130.161432.60431.021867.306.7910.28
acclimation light3625200900 µatm CO290023.31.52470147.940.0322401822655224309019267.8023.08819.70817.122108.58148.742280.402.343.55
acclimation light3625200900 µatm CO290023.31.52470147.940.03224018290152822814731267.8023.08819.70817.122108.58148.742280.402.343.55
dark362550150 µatm CO21503.80.3248798.570.0318411910.0000.00268.433.68130.57130.161432.60431.021867.306.7910.28
dark362550150 µatm CO21503.80.3248798.570.0318411931.6000.05268.433.68130.57130.161432.60431.021867.306.7910.28
dark362550150 µatm CO21503.80.3248798.570.0318411950.4500.04268.433.68130.57130.161432.60431.021867.306.7910.28
dark362550150 µatm CO21503.80.3248798.570.0318411970.3100.05268.433.68130.57130.161432.60431.021867.306.7910.28
dark362550150 µatm CO21503.80.3248798.570.0318411990.1900.03268.433.68130.57130.161432.60431.021867.306.7910.28
dark362550150 µatm CO21503.80.3248798.570.03184119110.0000.00268.433.68130.57130.161432.60431.021867.306.7910.28
dark362550150 µatm CO21503.80.3248798.570.0318411913268.433.68130.57130.161432.60431.021867.306.7910.28
dark362550900 µatm CO290023.31.52470147.940.0322401810.0100.02267.8023.08819.70817.122108.58148.742280.402.343.55
dark362550900 µatm CO290023.31.52470147.940.0322401833.0300.56267.8023.08819.70817.122108.58148.742280.402.343.55
dark362550900 µatm CO290023.31.52470147.940.0322401851.8200.03267.8023.08819.70817.122108.58148.742280.402.343.55
dark362550900 µatm CO290023.31.52470147.940.0322401870.9800.02267.8023.08819.70817.122108.58148.742280.402.343.55
dark362550900 µatm CO290023.31.52470147.940.0322401891.8200.21267.8023.08819.70817.122108.58148.742280.402.343.55
dark362550900 µatm CO290023.31.52470147.940.03224018110.0000.00267.8023.08819.70817.122108.58148.742280.402.343.55
dark362550900 µatm CO290023.31.52470147.940.0322401813267.8023.08819.70817.122108.58148.742280.402.343.55
dark3625200150 µatm CO21503.80.3248798.570.0318411910.8400.23268.433.68130.57130.161432.60431.021867.306.7910.28
dark3625200150 µatm CO21503.80.3248798.570.0318411939.4800.58268.433.68130.57130.161432.60431.021867.306.7910.28
dark3625200150 µatm CO21503.80.3248798.570.03184119515.4001.29268.433.68130.57130.161432.60431.021867.306.7910.28
dark3625200150 µatm CO21503.80.3248798.570.0318411977.7200.69268.433.68130.57130.161432.60431.021867.306.7910.28
dark3625200150 µatm CO21503.80.3248798.570.0318411992.2100.02268.433.68130.57130.161432.60431.021867.306.7910.28
dark3625200150 µatm CO21503.80.3248798.570.03184119110.5450.05268.433.68130.57130.161432.60431.021867.306.7910.28
dark3625200150 µatm CO21503.80.3248798.570.03184119130.0270.01268.433.68130.57130.161432.60431.021867.306.7910.28
dark3625200900 µatm CO290023.31.52470147.940.0322401811.1900.01267.8023.08819.70817.122108.58148.742280.402.343.55
dark3625200900 µatm CO290023.31.52470147.940.03224018311.6500.56267.8023.08819.70817.122108.58148.742280.402.343.55
dark3625200900 µatm CO290023.31.52470147.940.03224018519.2006.48267.8023.08819.70817.122108.58148.742280.402.343.55
dark3625200900 µatm CO290023.31.52470147.940.03224018716.7501.40267.8023.08819.70817.122108.58148.742280.402.343.55
dark3625200900 µatm CO290023.31.52470147.940.03224018913.3100.43267.8023.08819.70817.122108.58148.742280.402.343.55
dark3625200900 µatm CO290023.31.52470147.940.032240181114.2101.59267.8023.08819.70817.122108.58148.742280.402.343.55
dark3625200900 µatm CO290023.31.52470147.940.03224018130.1900.03267.8023.08819.70817.122108.58148.742280.402.343.55