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Oelmann, Yvonne; Wilcke, Wolfgang; Rosenkranz, Stephan; Lange, Markus; Steinbeiss, Sibylle; Habekost, Maike; Gleixner, Gerd; Luo, Guangjuan; Guderle, Marcus; Meyer, Sebastian Tobias (2015): Collection of data on particulate and dissolved soil nitrogen in the Jena Experiment (Main Experiment, time series since 2002) [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.854372

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Abstract:
This data set contains four time series of particulate and dissolved soil nitrogen measurements from the main experiment plots of a large grassland biodiversity experiment (the Jena Experiment; see further details below). In the main experiment, 82 grassland plots of 20 x 20 m were established from a pool of 60 species belonging to four functional groups (grasses, legumes, tall and small herbs). In May 2002, varying numbers of plant species from this species pool were sown into the plots to create a gradient of plant species richness (1, 2, 4, 8, 16 and 60 species) and functional richness (1, 2, 3, 4 functional groups). Plots were maintained by bi-annual weeding and mowing.
1. Total nitrogen from solid phase: Stratified soil sampling was performed every two years since before sowing in April 2002 and was repeated in April 2004, 2006 and 2008 to a depth of 30 cm segmented to a depth resolution of 5 cm giving six depth subsamples per core. In 2002 five samples per plot were taken and analyzed independently. Averaged values per depth layer are reported. In later years, three samples per plot were taken, pooled in the field, and measured as a combined sample. Sampling locations were less than 30 cm apart from sampling locations in other years. All soil samples were passed through a sieve with a mesh size of 2 mm in 2002. In later years samples were further sieved to 1 mm. No additional mineral particles were removed by this procedure. Total nitrogen concentration was analyzed on ball-milled subsamples (time 4 min, frequency 30 s-1) by an elemental analyzer at 1150°C (Elementaranalysator vario Max CN; Elementar Analysensysteme GmbH, Hanau, Germany).
2. Total nitrogen from solid phase (high intensity sampling): In block 2 of the Jena Experiment, soil samples were taken to a depth of 1m (segmented to a depth resolution of 5 cm giving 20 depth subsamples per core) with three replicates per block ever 5 years starting before sowing in April 2002. Samples were processed as for the more frequent sampling but were always analyzed independently and never pooled.
3. Mineral nitrogen from KCl extractions: Five soil cores (diameter 0.01 m) were taken at a depth of 0 to 0.15 m (and between 2002 and 2004 also at a depth of 0.15 to 0.3 m) of the mineral soil from each of the experimental plots at various times over the years. In addition also plots of the management experiment, that altered mowing frequency and fertilized subplots (see further details below) were sampled in some later years. Samples of the soil cores per plot (subplots in case of the management experiment) were pooled during each sampling campaign. NO3‐N and NH4‐N concentrations were determined by extraction of soil samples with 1 M KCl solution and were measured in the soil extract with a Continuous Flow Analyzer (CFA, 2003–2005: Skalar, Breda, Netherlands; 2006–2007: AutoAnalyzer, Seal, Burgess Hill, United Kingdom).
4. Dissolved nitrogen in soil solution: Glass suction plates with a diameter of 12 cm, 1 cm thickness and a pore size of 1–1.6 µm (UMS GmbH, Munich, Germany) were installed in April 2002 in depths of 10, 20, 30 and 60 cm to collect soil solution. The sampling bottles were continuously evacuated to a negative pressure between 50 and 350 mbar, such that the suction pressure was about 50 mbar above the actual soil water tension. Thus, only the soil leachate was collected. Cumulative soil solution was sampled biweekly and analyzed for nitrate (NO3-), ammonium (NH4+) and total dissolved nitrogen concentrations with a continuous flow analyzer (CFA, Skalar, Breda, The Netherlands). Nitrate was analyzed photometrically after reduction to NO2- and reaction with sulfanilamide and naphthylethylenediamine-dihydrochloride to an azo-dye. Our NO3- concentrations contained an unknown contribution of NO2- that is expected to be small. Simultaneously to the NO3- analysis, NH4+ was determined photometrically as 5-aminosalicylate after a modified Berthelot reaction. The detection limits of NO3- and NH4+ were 0.02 and 0.03 mg N L-1, respectively. Total dissolved N in soil solution was analyzed by oxidation with K2S2O8 followed by reduction to NO2- as described above for NO3-. Dissolved organic N (DON) concentrations in soil solution were calculated as the difference between TDN and the sum of mineral N (NO3- + NH4+).
Related to:
Plot information of the Jena Main Experiment
Project(s):
The Jena Experiment (JenExp)
Coverage:
Latitude: 50.946100 * Longitude: 11.611300
Date/Time Start: 2002-01-01T00:00:00 * Date/Time End: 2008-12-31T00:00:00
Comment:
This data set is an ongoing collection of soil nitrogen data from the Jena Experiment which is extended every year. Please refer to the datasets of the individual years below. Data is published 7 years after measuring. If you are interested in more recent years, please directly contact the authors via the Jena Experiment data manager [http://www.the-jena-experiment.de/Contact.html].
In case you want to cite this collection please use: "Yvonne Oelmann, Wolfgang Wilcke, Stephan Rosenkranz, Markus Lange, Sibylle Steinbeiß, Maike Habekost, Gerd Gleixner, Guangjuan Luo, Marcus Guderle, Sebastian T Meyer (2015): Collection of data on particulate and dissolved soil nitrogen in the Jena Experiment (Main Experiment, time series since 2002), doi:10.1594/PANGAEA.854372 (downloaded [DATE])".
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
16 datasets

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Datasets listed in this publication series

  1. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Dissolved nitrogen in soil solution of the Jena Experiment (Main Experiment, year 2002). https://doi.org/10.1594/PANGAEA.854342
  2. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Dissolved nitrogen in soil solution of the Jena Experiment (Main Experiment, year 2003). https://doi.org/10.1594/PANGAEA.854343
  3. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Dissolved nitrogen in soil solution of the Jena Experiment (Main Experiment, year 2004). https://doi.org/10.1594/PANGAEA.854344
  4. Steinbeiss, S; Habekost, M; Lange, M et al. (2015): Total nitrogen from solid phase in the Jena Experiment (Main Experiment up to 30cm depth, year 2002). https://doi.org/10.1594/PANGAEA.854357
  5. Steinbeiss, S; Lange, M; Gleixner, G (2015): Total nitrogen from solid phase in the Jena Experiment (Block 2 Main Experiment up to 100cm depth, year 2002). https://doi.org/10.1594/PANGAEA.854358
  6. Steinbeiss, S; Habekost, M; Lange, M et al. (2015): Total nitrogen from solid phase in the Jena Experiment (Main Experiment up to 30cm depth, year 2004). https://doi.org/10.1594/PANGAEA.854359
  7. Habekost, M; Lange, M; Gleixner, G (2015): Total nitrogen from solid phase in the Jena Experiment (Main Experiment up to 30cm depth, year 2006). https://doi.org/10.1594/PANGAEA.854360
  8. Steinbeiss, S; Lange, M; Gleixner, G (2015): Total nitrogen from solid phase in the Jena Experiment (Block 2 Main Experiment up to 100cm depth, year 2007). https://doi.org/10.1594/PANGAEA.854361
  9. Habekost, M; Lange, M; Gleixner, G (2015): Total nitrogen from solid phase in the Jena Experiment (Main Experiment up to 30cm depth, year 2008). https://doi.org/10.1594/PANGAEA.854362
  10. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Mineral nitrogen from KCl extractions of soil from the Jena Experiment (Main Experiment up to 30cm depth, year 2002). https://doi.org/10.1594/PANGAEA.854365
  11. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Mineral nitrogen from KCl extractions of soil from the Jena Experiment (Main Experiment up to 30cm depth, year 2003). https://doi.org/10.1594/PANGAEA.854366
  12. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Mineral nitrogen from KCl extractions of soil from the Jena Experiment (Main Experiment up to 30cm depth, year 2004). https://doi.org/10.1594/PANGAEA.854367
  13. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Mineral nitrogen from KCl extractions of soil from the Jena Experiment (Main Experiment up to 15cm depth, year 2005). https://doi.org/10.1594/PANGAEA.854368
  14. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Mineral nitrogen from KCl extractions of soil from the Jena Experiment (Main Experiment up to 15cm depth, year 2006). https://doi.org/10.1594/PANGAEA.854369
  15. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Mineral nitrogen from KCl extractions of soil from the Jena Experiment (Main Experiment up to 15cm depth, year 2007). https://doi.org/10.1594/PANGAEA.854370
  16. Oelmann, Y; Rosenkranz, S; Wilcke, W (2015): Mineral nitrogen from KCl extractions of soil from the Jena Experiment (Main Experiment up to 15cm depth, year 2008). https://doi.org/10.1594/PANGAEA.854371