Citation:

journal.pone.0055562.t001

Name: Seawater carbonate chemistry and photosystem II photoinactivation of the coastal diatom Thalassiosira pseudonana in a laboratory experiment
Published: 2013-12-17
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

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Published: 2013-12-17 • DOI registered: 2014-01-14

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Abstract:

We studied the interactive effects of pCO2 and growth light on the coastal marine diatom Thalassiosira pseudonana CCMP 1335 growing under ambient and expected end-of-the-century pCO2 (750 ppmv), and a range of growth light from 30 to 380 µmol photons/m**2/s. Elevated pCO2 significantly stimulated the growth of T. pseudonana under sub-saturating growth light, but not under saturating to super-saturating growth light. Under ambient pCO2 susceptibility to photoinactivation of photosystem II (sigma i) increased with increasing growth rate, but cells growing under elevated pCO2 showed no dependence between growth rate and sigma i, so under high growth light cells under elevated pCO2 were less susceptible to photoinactivation of photosystem II, and thus incurred a lower running cost to maintain photosystem II function. Growth light altered the contents of RbcL (RUBISCO) and PsaC (PSI) protein subunits, and the ratios among the subunits, but there were only limited effects on these and other protein pools between cells grown under ambient and elevated pCO2.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4 [webpage]. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Identification	ID		Campbell, Douglas A
2	Species	Species		Campbell, Douglas A
3	Treatment	Treat		Campbell, Douglas A
4	Replicates	Repl	#	Campbell, Douglas A
5	Date	Date		Campbell, Douglas A		date of first shift experiment
6	Date	Date		Campbell, Douglas A		date of last shift experiment
7	Irradiance	E	µmol/m²/s	Campbell, Douglas A		growth light
8	Irradiance	E	µmol/m²/s	Campbell, Douglas A		high light shift
9	Time in minutes	Time	min	Campbell, Douglas A		light shift duration
10	Cell density	Cells	#/ml	Campbell, Douglas A
11	Cell biovolume	Cell biovol	µm³	Campbell, Douglas A
12	Growth rate	µ	1/day	Campbell, Douglas A
13	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured in the dark
14	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured at growth light
15	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured at light shift intensity
16	Photochemical quenching	qP		Campbell, Douglas A
17	Protein per cell	Protein/cell	pg/#	Campbell, Douglas A
18	Photosynthetic protein PsbA	PsbA	pmol/µg	Campbell, Douglas A
19	Photosynthetic protein PsbD	PsbD	pmol/µg	Campbell, Douglas A
20	Photosynthetic protein Rubisco	RbcL	pmol/mg	Campbell, Douglas A
21	CF1 subunit of ATP synthase protein	AtpB	pmol/mg	Campbell, Douglas A
22	Cytochrome c1	PetC	pmol/µg	Campbell, Douglas A
23	Chlorophyll a per cell	Chl a/cell	fmol/#	Campbell, Douglas A	Photospectrometer, methylene blue
24	Carbon, organic, particulate, per cell	POC/cell	pg/#	Campbell, Douglas A	Carbon and nitrogen and sulfur (CNS) element analyzer, Elementar, Vario EL
25	Nitrogen, organic, particulate, per cell	PON/cell	pg/#	Campbell, Douglas A	Carbon and nitrogen and sulfur (CNS) element analyzer, Elementar, Vario EL
26	Carbon/Nitrogen ratio	C/N		Campbell, Douglas A
27	Temperature, water	Temp	°C	Campbell, Douglas A
28	Salinity	Sal		Campbell, Douglas A
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Campbell, Douglas A
30	pH, NBS scale	pH NBS		Campbell, Douglas A	Potentiometric titration	NBS scale
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Campbell, Douglas A	Calculated using CO2SYS
32	Alkalinity, total	AT	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
33	Bicarbonate ion	[HCO3]-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
34	Carbonate ion	[CO3]2-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
35	Carbon dioxide	CO2	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
36	Phosphate	[PO4]3-	µmol/l	Campbell, Douglas A
37	Silicate	Si(OH)4	µmol/l	Campbell, Douglas A
38	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	pH, total scale	pHT		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1542 data points

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Datasets with similar metadata

Li, W; Wang, T; Campbell, DA et al. (2020): Seawater carbonate chemistry and growth and particulate organic nitrogen production of diatom Thalassiosira pseudonana. https://doi.org/10.1594/PANGAEA.922459
Qu, L; Campbell, DA; Gao, K (2021): Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom. https://doi.org/10.1594/PANGAEA.928761
Laws, EA; McClellan, SA (2020): Seawater carbonate chemistry and growth and physiology of the marine diatom Thalassiosira pseudonana (Coscinodiscophyceae). https://doi.org/10.1594/PANGAEA.929551

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