Citation:

journal.pone.0055562.t001

Name: Seawater carbonate chemistry and photosystem II photoinactivation of the coastal diatom Thalassiosira pseudonana in a laboratory experiment
Published: 2013-12-17
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

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Abstract:

We studied the interactive effects of pCO2 and growth light on the coastal marine diatom Thalassiosira pseudonana CCMP 1335 growing under ambient and expected end-of-the-century pCO2 (750 ppmv), and a range of growth light from 30 to 380 µmol photons/m**2/s. Elevated pCO2 significantly stimulated the growth of T. pseudonana under sub-saturating growth light, but not under saturating to super-saturating growth light. Under ambient pCO2 susceptibility to photoinactivation of photosystem II (sigma i) increased with increasing growth rate, but cells growing under elevated pCO2 showed no dependence between growth rate and sigma i, so under high growth light cells under elevated pCO2 were less susceptible to photoinactivation of photosystem II, and thus incurred a lower running cost to maintain photosystem II function. Growth light altered the contents of RbcL (RUBISCO) and PsaC (PSI) protein subunits, and the ratios among the subunits, but there were only limited effects on these and other protein pools between cells grown under ambient and elevated pCO2.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Identification	ID		Campbell, Douglas A
2	Species	Species		Campbell, Douglas A
3	Treatment	Treat		Campbell, Douglas A
4	Replicates	Repl	#	Campbell, Douglas A
5	Date	Date		Campbell, Douglas A		date of first shift experiment
6	Date	Date		Campbell, Douglas A		date of last shift experiment
7	Irradiance	E	µmol/m²/s	Campbell, Douglas A		growth light
8	Irradiance	E	µmol/m²/s	Campbell, Douglas A		high light shift
9	Time in minutes	Time	min	Campbell, Douglas A		light shift duration
10	Cell density	Cells	#/ml	Campbell, Douglas A
11	Cell biovolume	Cell biovol	µm³	Campbell, Douglas A
12	Growth rate	µ	1/day	Campbell, Douglas A
13	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured in the dark
14	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured at growth light
15	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured at light shift intensity
16	Photochemical quenching	qP		Campbell, Douglas A
17	Protein per cell	Protein/cell	pg/#	Campbell, Douglas A
18	Photosynthetic protein PsbA	PsbA	pmol/µg	Campbell, Douglas A
19	Photosynthetic protein PsbD	PsbD	pmol/µg	Campbell, Douglas A
20	Photosynthetic protein Rubisco	RbcL	pmol/mg	Campbell, Douglas A
21	CF1 subunit of ATP synthase protein	AtpB	pmol/mg	Campbell, Douglas A
22	Cytochrome c1	PetC	pmol/µg	Campbell, Douglas A
23	Chlorophyll a per cell	Chl a/cell	fmol/#	Campbell, Douglas A	Photospectrometer, methylene blue
24	Carbon, organic, particulate, per cell	POC/cell	pg/#	Campbell, Douglas A	Carbon and nitrogen and sulfur (CNS) element analyzer, Elementar, Vario EL
25	Nitrogen, organic, particulate, per cell	PON/cell	pg/#	Campbell, Douglas A	Carbon and nitrogen and sulfur (CNS) element analyzer, Elementar, Vario EL
26	Carbon/Nitrogen ratio	C/N		Campbell, Douglas A
27	Temperature, water	Temp	°C	Campbell, Douglas A
28	Salinity	Sal		Campbell, Douglas A
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Campbell, Douglas A
30	pH	pH		Campbell, Douglas A	Potentiometric titration	NBS scale
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Campbell, Douglas A	Calculated using CO2SYS
32	Alkalinity, total	AT	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
33	Bicarbonate ion	[HCO3]-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
34	Carbonate ion	[CO3]2-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
35	Carbon dioxide	CO2	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
36	Phosphate	[PO4]3-	µmol/l	Campbell, Douglas A
37	Silicate	Si(OH)4	µmol/l	Campbell, Douglas A
38	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1542 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 ID	2 Species	3 Treat	4 Repl [#]	5 Date (date of first shift experiment)	6 Date (date of last shift experiment)	7 E [µmol/m²/s] (growth light)	8 E [µmol/m²/s] (high light shift)	9 Time [min] (light shift duration)	10 Cells [#/ml]	11 Cell biovol [µm³]	12 µ [1/day]	13 sigma PSII [A²/quanta] (measured in the dark)	14 sigma PSII [A²/quanta] (measured at growth light)	15 sigma PSII [A²/quanta] (measured at light shift inten...)	16 qP	17 Protein/cell [pg/#]	18 PsbA [pmol/µg]	19 PsbD [pmol/µg]	20 RbcL [pmol/mg]	21 AtpB [pmol/mg]	22 PetC [pmol/µg]	23 Chl a/cell [fmol/#] (Photospectrometer, methylene ...)	24 POC/cell [pg/#] (Carbon and nitrogen and sulfu...)	25 PON/cell [pg/#] (Carbon and nitrogen and sulfu...)	26 C/N	27 Temp [°C]	28 Sal	29 DIC [µmol/kg]	30 pH (NBS scale, Potentiometric tit...)	31 pCO2water_SST_wet [ppmv] (Calculated using CO2SYS)	32 AT [µmol/kg] (Calculated using CO2SYS)	33 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	34 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	35 CO2 [µmol/kg] (Calculated using CO2SYS)	36 [PO4]3- [µmol/l]	37 Si(OH)4 [µmol/l]	38 CSC flag (Calculated using seacarb afte...)	39 pH (total scale, Calculated using...)	40 CO2 [µmol/kg] (Calculated using seacarb afte...)	41 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	42 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	43 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	44 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	45 AT [µmol/kg] (Calculated using seacarb afte...)	46 Omega Arg (Calculated using seacarb afte...)	47 Omega Cal (Calculated using seacarb afte...)
95	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	1	2011-12-25	2012-05-31	31	450	90	1298400	25.882	0.744	351.754		0.000068	0.529	3.927	0.057	0.074		1.012	0.899	0.242	6.006	1.206	4.981	18	35	1976	8.14	312	2302	1750	215	10.7	21	52.5	27	8.02	14.01	410	409	1805	157	2221	2.43	3.76
95	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	2	2011-12-25	2012-05-31	31	450	90	426840	17.206	1.000	307.879	303.665	0.000078	0.402	4.703	0.067	0.063	0.030	0.719	1.040	0.191	7.897	1.644	4.802	18	35	1976	8.14	312	2302	1750	215	10.7	21	52.5	27	8.02	14.01	410	409	1805	157	2221	2.43	3.76
95	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	3	2011-12-25	2012-05-31	31	450	90	249680	20.398	1.040	322.927	313.657	0.000068	0.389	8.612	0.068	0.050	0.033	0.583	1.108	0.394	8.764	1.602	5.470	18	35	1976	8.14	312	2302	1750	215	10.7	21	52.5	27	8.02	14.01	410	409	1805	157	2221	2.43	3.76
95	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	4	2011-12-25	2012-05-31	31	450	90	169360	20.398	0.703	326.957	315.623	0.000069	0.359	9.241	0.052	0.100	0.041	1.046	1.369	0.372	6.974	1.583	4.406	18	35	1976	8.14	312	2302	1750	215	10.7	21	52.5	27	8.02	14.01	410	409	1805	157	2221	2.43	3.76
96	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	1	2011-12-22	2012-05-27	81	450	90	347660	25.003	1.554	305.086		0.000086	0.532	5.122	0.074	0.050	0.071	1.464	0.659	0.176	7.343	1.328	5.531	18	35	1866	8.18	300	2212	1632	209	9.1	21	52.5	27	8.06	11.98	351	349	1692	162	2124	2.50	3.86
96	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	2	2011-12-22	2012-05-27	81	450	90	555040	21.092	1.283	298.470		0.000086	0.583	3.816	0.031	0.047	0.045	0.838	1.123	0.128	6.934	1.202	5.768	18	35	1866	8.18	300	2212	1632	209	9.1	21	52.5	27	8.06	11.98	351	349	1692	162	2124	2.50	3.86
96	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	3	2011-12-22	2012-05-27	81	450	90	314280	22.584	1.267	292.231	262.718	0.000081	0.397	6.869	0.056	0.038		0.604	0.574	0.275	10.823	1.907	5.676	18	35	1866	8.18	300	2212	1632	209	9.1	21	52.5	27	8.06	11.98	351	349	1692	162	2124	2.50	3.86
96	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	4	2011-12-22	2012-05-27	81	450	90	420160	24.186	1.582	298.886	314.576	0.000096	0.477	6.697	0.047	0.076	0.049	1.221	0.913	0.211	8.132	1.465	5.551	18	35	1866	8.18	300	2212	1632	209	9.1	21	52.5	27	8.06	11.98	351	349	1692	162	2124	2.50	3.86
97	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	1	2011-12-21	2012-05-27	167	450	90	320520	23.366	1.748	266.720		0.000109		5.586	0.069	0.063	0.060	1.506	0.521	0.127	7.297	1.191	6.126	18	35	1902	8.10	332	2196	1700	191	11.4	21	52.5	27	7.98	14.88	436	434	1748	139	2122	2.15	3.32
97	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	2	2011-12-21	2012-05-27	167	450	90	603940	25.003	1.599	269.316	240.897	0.000095	0.601	4.201	0.066	0.071	0.064	1.529	0.859	0.104	6.999	1.311	5.339	18	35	1902	8.10	332	2196	1700	191	11.4	21	52.5	27	7.98	14.88	436	434	1748	139	2122	2.15	3.32
97	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	3	2011-12-21	2012-05-27	167	450	90	497780	25.840	1.664	261.564	237.114	0.000093	0.455	3.818	0.067	0.081	0.077	2.339	0.427	0.119	7.248	1.276	5.679	18	35	1902	8.10	332	2196	1700	191	11.4	21	52.5	27	7.98	14.88	436	434	1748	139	2122	2.15	3.32
97	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	4	2011-12-21	2012-05-27	167	450	90	409480	27.612	1.842	267.192	266.227	0.000091	0.570	5.423	0.079	0.105	0.077	1.992	0.522	0.187	8.647	1.369	6.318	18	35	1902	8.10	332	2196	1700	191	11.4	21	52.5	27	7.98	14.88	436	434	1748	139	2122	2.15	3.32
98	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	1	2012-05-17	2012-05-17	239	450	90	444500	28.663	1.332	240.097	200.564	0.000081	0.554	4.845	0.101	0.049	0.077	2.361	0.491	0.115	8.546	1.582	5.402	18	35	1908	8.10	321	2110	1630	184	11.0	21	52.5	27	7.98	14.93	437	435	1754	140	2128	2.15	3.33
98	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	2	2012-05-17	2012-05-17	239	450	90	474820	26.781	1.561	232.825	232.677	0.000085	0.617	6.266	0.165	0.086	0.084	1.748	0.716	0.140	8.580	1.507	5.694	18	35	1908	8.10	321	2110	1630	184	11.0	21	52.5	27	7.98	14.93	437	435	1754	140	2128	2.15	3.33
98	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	3	2012-05-17	2012-05-17	239	450	90	598160	26.781	1.517	245.011	228.986	0.000086	0.685	4.390	0.072	0.058	0.069	1.679	0.502	0.085	7.229	1.338	5.402	18	35	1908	8.10	321	2110	1630	184	11.0	21	52.5	27	7.98	14.93	437	435	1754	140	2128	2.15	3.33
99	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	1	2012-04-29	2012-04-29	375	450	90	971240	26.781	1.303	212.021	156.172	0.000074	0.659	5.146	0.083	0.036	0.071	1.779	0.447	0.103	7.000	1.373	5.098	18	35	1947	8.16	295	2286	1714	223	10.1	21	52.5	27	8.04	13.14	385	383	1772	162	2201	2.50	3.86
99	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	2	2012-04-29	2012-04-29	375	450	90	767140	25.882	1.335	208.997	154.511	0.000070	0.620	4.639	0.081	0.078	0.075	2.166	0.845	0.080	6.835	1.332	5.130	18	35	1947	8.16	295	2286	1714	223	10.1	21	52.5	27	8.04	13.14	385	383	1772	162	2201	2.50	3.86
99	Thalassiosira pseudonana (phytoplankton)	pCO2=390 ppmv	3	2012-04-29	2012-04-29	375	450	90	641520	27.700	1.380	202.216	157.361	0.000074	0.665	6.416	0.114	0.076	0.072	1.654	0.469	0.135	7.538	1.544	4.881	18	35	1947	8.16	295	2286	1714	223	10.1	21	52.5	27	8.04	13.14	385	383	1772	162	2201	2.50	3.86
100	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	1	2012-01-27	2012-04-16	31	450	90	254760	15.017	1.558	308.095	312.231	0.000065	0.292	11.618	0.059	0.037	0.051	1.057	0.360	0.280	8.769	3.625	2.419	18	35	2007	7.92	551	2213	1850	138	18.9	21	52.5	27	7.80	24.28	711	708	1884	99	2155	1.53	2.36
100	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	2	2012-01-27	2012-04-16	31	450	90	283340	20.398	1.351	319.211	305.165	0.000068	0.517	6.478	0.109	0.050	0.044	1.115	0.724	0.216	7.277	1.424	5.111	18	35	2007	7.92	551	2213	1850	138	18.9	21	52.5	27	7.80	24.28	711	708	1884	99	2155	1.53	2.36
100	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	3	2012-01-27	2012-04-16	31	450	90	251680	18.409	1.262	295.545	302.638	0.000074	0.372	8.934	0.031	0.069	0.037	1.445	0.798	0.290	8.018	1.543	5.197	18	35	2007	7.92	551	2213	1850	138	18.9	21	52.5	27	7.80	24.28	711	708	1884	99	2155	1.53	2.36
101	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	1	2012-01-25	2012-04-12	81	450	90	562040	17.206	1.670	295.545		0.000063	0.442	4.050	0.050	0.054	0.064	1.254	0.623	0.139	6.582	1.304	5.046	18	35	2047	7.85	656	2226	1903	121	22.5	21	52.5	27	7.73	29.25	856	853	1931	86	2170	1.33	2.06
101	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	2	2012-01-25	2012-04-12	81	450	90	427640	22.584	1.952	311.770	255.541	0.000069	0.436	5.467	0.061	0.041	0.056	1.327	0.756	0.159	8.401	1.522	5.519	18	35	2047	7.85	656	2226	1903	121	22.5	21	52.5	27	7.73	29.25	856	853	1931	86	2170	1.33	2.06
101	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	3	2012-01-25	2012-04-12	81	450	90	344120	22.584	1.370	264.437	283.796	0.000071	0.473	7.416	0.057	0.054	0.040	1.442	0.535	0.222	8.213	1.530	5.368	18	35	2047	7.85	656	2226	1903	121	22.5	21	52.5	27	7.73	29.25	856	853	1931	86	2170	1.33	2.06
102	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	1	2012-01-24	2012-04-11	167	450	90	674860	19.720	1.943	264.437		0.000068	0.497	4.126	0.083	0.055	0.076	2.179	0.430	0.103	7.322	1.317	5.559	18	35	2024	7.82	706	2186	1889	111	24.2	21	52.5	27	7.70	31.05	909	906	1913	80	2136	1.23	1.91
102	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	2	2012-01-24	2012-04-11	167	450	90	682280	25.003	2.309	308.095	216.857	0.000068	0.550	3.770	0.083	0.060	0.086	1.391	0.710	0.112	7.044	1.272	5.538	18	35	2024	7.82	706	2186	1889	111	24.2	21	52.5	27	7.70	31.05	909	906	1913	80	2136	1.23	1.91
102	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	3	2012-01-24	2012-04-11	167	450	90	461560	25.882	2.213	319.211	239.267	0.000073	0.494	5.836	0.070	0.083	0.063	2.286	0.543	0.170	9.025	1.410	6.400	18	35	2024	7.82	706	2186	1889	111	24.2	21	52.5	27	7.70	31.05	909	906	1913	80	2136	1.23	1.91
103	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	1	2012-05-08	2012-05-09	245	450	90	467280	26.781	1.451	311.770	211.768	0.000076	0.522	4.371	0.115	0.068	0.074	1.790	0.440	0.117	13.322	2.545	5.234	18	35	2064	7.86	639	2282	1916	126	22.0	21	52.5	27	7.74	28.80	843	840	1946	89	2191	1.37	2.13
103	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	2	2012-05-08	2012-05-09	245	450	90	815320	21.092	1.421	278.611	205.210	0.000070	0.780	3.674	0.134	0.087	0.069	2.610	0.582	0.081	10.349	2.122	4.878	18	35	2064	7.86	639	2282	1916	126	22.0	21	52.5	27	7.74	28.80	843	840	1946	89	2191	1.37	2.13
103	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	3	2012-05-08	2012-05-09	245	450	90	757000	26.781	1.615	311.344	222.427	0.000071	0.672	3.947	0.087	0.068	0.061	1.889	0.424	0.085	6.563	1.133	5.793	18	35	2064	7.86	639	2282	1916	126	22.0	21	52.5	27	7.74	28.80	843	840	1946	89	2191	1.37	2.13
104	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	1	2012-04-21	2012-04-22	384	450	90	783140	28.663	1.310	291.453	185.117	0.000068	0.675	5.084	0.098	0.051	0.072	2.431	0.355	0.106	4.238	0.774	5.477	18	35	2152	7.82	745	2320	2007	119	25.5	21	52.5	27	7.70	33.01	966	963	2034	85	2267	1.31	2.03
104	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	2	2012-04-21	2012-04-22	384	450	90	889880	28.663	1.089	282.039	175.058	0.000065	0.656	6.082	0.090	0.070	0.071	2.067	0.339	0.118	4.730	0.955	4.952	18	35	2152	7.82	745	2320	2007	119	25.5	21	52.5	27	7.70	33.01	966	963	2034	85	2267	1.31	2.03
104	Thalassiosira pseudonana (phytoplankton)	pCO2=750 ppmv	3	2012-04-21	2012-04-22	384	450	90	527680	29.648	1.113	239.070	199.683	0.000071	0.662	6.270	0.117	0.072	0.072	1.974	0.352	0.106	10.121	1.708	5.927	18	35	2152	7.82	745	2320	2007	119	25.5	21	52.5	27	7.70	33.01	966	963	2034	85	2267	1.31	2.03