Citation:

meps09644

Name: Seawater carbonate chemistry, biomass and metabolic rates (leucine incorporation, CO2 fixation and respiration) of Rhodobacteraceae (strain MED165) and Flavobacteriaceae (strain MED217) in a laboratory experiment
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cytophaga sp.; Growth/Morphology; Heterotrophic prokaryotes; Laboratory experiment; Laboratory strains; Mediterranean Sea; Other metabolic rates; Pelagos; Respiration; Single species

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Abstract:

Experimental results related to the effects of ocean acidification on planktonic marine microbes are still rather inconsistent and occasionally contradictory. Moreover, laboratory or field experiments that address the effects of changes in CO2 concentrations on heterotrophic microbes are very scarce, despite the major role of these organisms in the marine carbon cycle. We tested the direct effect of an elevated CO2 concentration (1000 ppmv) on the biomass and metabolic rates (leucine incorporation, CO2 fixation and respiration) of 2 isolates belonging to 2 relevant marine bacterial families, Rhodobacteraceae (strain MED165) and Flavobacteriaceae (strain MED217). Our results demonstrate that, contrary to some expectations, high pCO2 did not negatively affect bacterial growth but increased growth efficiency in the case of MED217. The elevated partial pressure of CO2 (pCO2) caused, in both cases, higher rates of CO2 fixation in the dissolved fraction and, in the case of MED217, lower respiration rates. Both responses would tend to increase the pH of seawater acting as a negative feedback between elevated atmospheric CO2 concentrations and ocean acidification.

Keyword(s):

Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cytophaga sp.; Growth/Morphology; Heterotrophic prokaryotes; Laboratory experiment; Laboratory strains; Mediterranean Sea; Other metabolic rates; Pelagos; Respiration; Single species

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-04-01.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Teira, Eva
2	Strain	Strain		Teira, Eva
3	Treatment	Treat		Teira, Eva
4	pH	pH		Teira, Eva	Potentiometric	total scale
5	pH, standard error	pH std e	±	Teira, Eva	Potentiometric	total scale
6	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Teira, Eva	Calculated
7	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Teira, Eva	Calculated
8	Carbon, inorganic, dissolved	DIC	µmol/kg	Teira, Eva
9	Carbon, inorganic, dissolved, standard error	DIC std e	±	Teira, Eva
10	Alkalinity, total	AT	µmol/kg	Teira, Eva	Calculated
11	Alkalinity, total, standard error	AT std e	±	Teira, Eva	Calculated
12	Temperature, water	Temp	°C	Teira, Eva
13	Temperature, water, standard error	T std e	±	Teira, Eva
14	Salinity	Sal		Teira, Eva
15	Salinity, standard error	Sal std e	±	Teira, Eva
16	Fluorescence, dissolved organic matter	fDOM	µg/l	Teira, Eva		humic-like
17	Fluorescence, dissolved organic matter, standard error	fDOM std e	±	Teira, Eva		humic-like
18	Fluorescence, particulate organic matter	FPOM	µg/l	Teira, Eva		protein-like
19	Fluorescence, particulate organic matter, standard error	FPOM std e	±	Teira, Eva		protein-like
20	Fluorescence, dissolved organic matter	fDOM	µg/l	Teira, Eva		protein-like
21	Fluorescence, dissolved organic matter, standard error	fDOM std e	±	Teira, Eva		protein-like
22	Abundance per volume	Abund v	#/ml	Teira, Eva
23	Abundance, standard error	Abund std e	±	Teira, Eva
24	Leucine incorporation rate	Leu inc rate	pmol/l/h	Teira, Eva
25	Leucine incorporation rate, standard error	Leu inc rate std e	±	Teira, Eva
26	Respiration rate, carbon dioxide	Resp CO2	µmol/l/day	Teira, Eva
27	Respiration rate, carbon dioxide, standard error	Resp CO2 std e	±	Teira, Eva
28	Bacteria, growth efficiency	Bact growth eff	%	Teira, Eva
29	Bacteria, growth efficiency, standard error	Bact growth eff std e	±	Teira, Eva
30	Carbon fixation rate	C fix	µmol/l/h	Teira, Eva		measured in the dissolved fraction rates
31	Carbon fixation rate, standard deviation	C fix std dev	±	Teira, Eva		measured in the dissolved fraction rates
32	Carbon fixation rate	C fix	µmol/l/h	Teira, Eva		measured in the particulate fraction rates
33	Carbon fixation rate, standard deviation	C fix std dev	±	Teira, Eva		measured in the particulate fraction rates
34	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

168 data points

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