Citation:

PANGAEA.928761

Name: Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom
Published: 2021-03-05
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira weissflogii

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Published: 2021-03-05 • DOI registered: 2021-04-03

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Abstract:

Diatom responses to ocean acidification have been documented with variable and controversial results. We grew the coastal diatom Thalassiosira weissflogii under 410 (LC, pH 8.13) vs 1000 μatm (HC, pH 7.83) pCO2 and at different levels of light (80, 140, 220 μmol photons/m**2/s), and found that light level alters physiological responses to OA. CO2 concentrating mechanisms (CCMs) were down-regulated in the HC-grown cells across all the light levels, as reflected by lowered activity of the periplasmic carbonic anhydrase and decreased photosynthetic affinity for CO2 or dissolved inorganic carbon. The specific growth rate was, however, enhanced significantly by 9.2% only at the limiting low light level. These results indicate that rather than CO2 “fertilization”, the energy saved from down-regulation of CCMs promoted the growth rate of the diatom when light availability is low, in parallel with enhanced respiration under OA to cope with the acidic stress by providing extra energy.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira weissflogii

Supplement to:

Qu, Liming; Campbell, Douglas A; Gao, Kunshan (2021): Ocean acidification interacts with growth light to suppress CO2 acquisition efficiency and enhance mitochondrial respiration in a coastal diatom. Marine Pollution Bulletin, 163, 112008, https://doi.org/10.1016/j.marpolbul.2021.112008

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2020): seacarb: seawater carbonate chemistry with R. R package version 3.2.14. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-01-28.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Gao, Kunshan		study
2	Species	Species		Gao, Kunshan
3	Registration number of species	Reg spec no		Gao, Kunshan
4	Uniform resource locator/link to reference	URL ref		Gao, Kunshan		WoRMS Aphia ID
5	Treatment	Treat		Gao, Kunshan
6	Treatment	Treat		Gao, Kunshan		Light
7	Growth rate	µ	1/day	Gao, Kunshan
8	Growth rate, standard deviation	µ std dev	±	Gao, Kunshan
9	Cell size	Cell size	µm	Gao, Kunshan
10	Cell size, standard deviation	Cell size std dev	±	Gao, Kunshan
11	Chlorophyll a per cell	Chl a/cell	pg/#	Gao, Kunshan
12	Chlorophyll a, standard deviation	Chl a std dev	±	Gao, Kunshan
13	Carotenoids per cell	Carotenoids/cell	pg/#	Gao, Kunshan
14	Carotenoids, standard deviation	Carotenoids std dev	±	Gao, Kunshan
15	Carotenoids/Chlorophyll a ratio	Carotenoids/Chl a		Gao, Kunshan
16	Carotenoids/Chlorophyll a ratio, standard deviation	Carotenoids/Chl a std dev	±	Gao, Kunshan
17	Maximum quantum yield of photosystem II	Fv/Fm		Gao, Kunshan
18	Maximum quantum yield of photosystem II, standard deviation	Fv/Fm std dev	±	Gao, Kunshan
19	Electron transport rate, relative	rETR		Gao, Kunshan
20	Electron transport rate, relative, standard deviation	rETR std dev	±	Gao, Kunshan
21	Non photochemical quenching	NPQ		Gao, Kunshan
22	Non photochemical quenching, standard deviation	NPQ std dev	±	Gao, Kunshan
23	Maximal electron transport rate, relative	rETR max		Gao, Kunshan
24	Maximal electron transport rate, relative, standard deviation	rETR max std dev	±	Gao, Kunshan
25	Electron transport rate efficiency	alpha		Gao, Kunshan
26	Electron transport rate efficiency, standard deviation	alpha std dev	±	Gao, Kunshan
27	Light saturation point	Ik		Gao, Kunshan
28	Light saturation point, standard deviation	Ik std dev	±	Gao, Kunshan
29	Effective quantum yield	Y		Gao, Kunshan
30	Effective quantum yield, standard deviation	Y std dev	±	Gao, Kunshan
31	Maximal electron transport rate, relative	rETR max	µmol e/m²/s	Gao, Kunshan		at growth light levels
32	Maximal electron transport rate, relative, standard deviation	rETR max std dev	±	Gao, Kunshan		at growth light levels
33	Carbon, inorganic, dissolved	DIC	mmol/l	Gao, Kunshan		for the half-maximal photosynthetic rate, at growth light levels
34	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Gao, Kunshan		for the half-maximal photosynthetic rate, at growth light levels
35	Ratio	Ratio		Gao, Kunshan		rETRmax/K(1/2), at growth light levels
36	Ratio, standard deviation	Ratio std dev	±	Gao, Kunshan		rETRmax/K(1/2), at growth light levels
37	Maximal electron transport rate, relative	rETR max		Gao, Kunshan		at saturated light levels
38	Maximal electron transport rate, relative, standard deviation	rETR max std dev	±	Gao, Kunshan		at saturated light levels
39	Carbon, inorganic, dissolved	DIC	mmol/l	Gao, Kunshan		for the half-maximal photosynthetic rate, at saturated light levels
40	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Gao, Kunshan		for the half-maximal photosynthetic rate, at saturated light levels
41	Ratio	Ratio		Gao, Kunshan		rETRmax/K(1/2), at saturated light levels
42	Ratio, standard deviation	Ratio std dev	±	Gao, Kunshan		rETRmax/K(1/2), at saturated light levels
43	Net photosynthesis rate, oxygen, per cell	PN O2/cell	µmol/#/h	Gao, Kunshan
44	Net photosynthesis rate, standard deviation	PN std dev	±	Gao, Kunshan
45	Respiration rate, oxygen, per cell	Resp O2/cell	µmol/#/h	Gao, Kunshan
46	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Gao, Kunshan
47	Oxygen evolution, daytime	O2 ev daytime	µmol/µg/day	Gao, Kunshan
48	Oxygen evolution, daytime, standard deviation	O2 ev daytime std dev	±	Gao, Kunshan
49	Net photosynthesis rate, oxygen, per chlorophyll a	PN O2/Chl a	µmol/µg/h	Gao, Kunshan
50	Net photosynthesis rate, standard deviation	PN std dev	±	Gao, Kunshan
51	Respiration rate, oxygen, per chlorophyll a	Resp O2/Chl a	µmol/µg/h	Gao, Kunshan
52	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Gao, Kunshan
53	Oxygen evolution per cell, daytime	O2 ev/cell daytime	µmol/#/h	Gao, Kunshan
54	Oxygen evolution, daytime, standard deviation	O2 ev daytime std dev	±	Gao, Kunshan
55	Extracellular carbonic anhydrase activity, per cell	eCA act/cell	U/#	Gao, Kunshan
56	Extracellular carbonic anhydrase activity, standard deviation	eCA act std dev	±	Gao, Kunshan
57	Carbon, inorganic, dissolved	DIC	mmol/l	Gao, Kunshan
58	Electron transport rate, relative	rETR		Gao, Kunshan		at growth light levels
59	Electron transport rate, relative, standard deviation	rETR std dev	±	Gao, Kunshan		at growth light levels
60	Electron transport rate, relative	rETR		Gao, Kunshan		at saturated light levels
61	Electron transport rate, relative, standard deviation	rETR std dev	±	Gao, Kunshan		at saturated light levels
62	Time in days	Time	days	Gao, Kunshan
63	Growth rate	µ	1/day	Gao, Kunshan
64	Salinity	Sal		Gao, Kunshan
65	Temperature, water	Temp	°C	Gao, Kunshan
66	pH, total scale	pHT		Gao, Kunshan		total scale
67	pH, standard deviation	pH std dev	±	Gao, Kunshan		total scale
68	Alkalinity, total	AT	µmol/kg	Gao, Kunshan
69	Alkalinity, total, standard deviation	AT std dev	±	Gao, Kunshan
70	Carbon, inorganic, dissolved	DIC	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
71	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Gao, Kunshan	Calculated using CO2SYS
72	Bicarbonate ion	[HCO3]-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
73	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Gao, Kunshan	Calculated using CO2SYS
74	Carbonate ion	[CO3]2-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
75	Carbonate ion, standard deviation	[CO3]2- std dev	±	Gao, Kunshan	Calculated using CO2SYS
76	Carbon dioxide	CO2	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
77	Carbon dioxide, standard deviation	CO2 std dev	±	Gao, Kunshan	Calculated using CO2SYS
78	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
79	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
80	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
81	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
82	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
83	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
84	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
85	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
86	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
87	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
88	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
89	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
90	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
91	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
92	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
93	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
94	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4428 data points

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Li, W; Wang, T; Campbell, DA et al. (2020): Seawater carbonate chemistry and growth and particulate organic nitrogen production of diatom Thalassiosira pseudonana. https://doi.org/10.1594/PANGAEA.922459
Qu, L; Beardall, J; Jiang, X et al. (2021): Seawater carbonate chemistry and specific growth rate, respiration rate, net photosynthetic rate and photochemical parameters of Phaeodactylum tricornutum. https://doi.org/10.1594/PANGAEA.939406
Liu, N; Beardall, J; Gao, K (2017): Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum. https://doi.org/10.1594/PANGAEA.900658

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