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Bibby, Ruth; Widdicombe, Stephen; Parry, Helen E; Spicer, John I; Pipe, R (2008): Seawater carbonate chemistry during experiments with Mytilus edulis, 2008. PANGAEA, https://doi.org/10.1594/PANGAEA.718100, Supplement to: Bibby, R et al. (2008): Effects of ocean acidification on the immune response of the blue mussel Mytilus edulis. Aquatic Biology, 2(1), 97-74, https://doi.org/10.3354/ab00037

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Abstract:
The effects of medium term (32 d) hypercapnia on the immune response of Mytilus edulis were investigated in mussels exposed to acidified (using CO2) sea water (pH 7.7, 7.5 or 6.7; control: pH 7.8). Levels of phagocytosis increased significantly during the exposure period, suggesting an immune response induced by the experimental set-up. However, this induced stress response was suppressed when mussels were exposed to acidified sea water. Acidified sea water did not have any significant effects on other immuno-surveillance parameters measured (superoxide anion production, total and differential cell counts). These results suggest that ocean acidification may impact the physiological condition and functionality of the haemocytes and could have a significant effect on cellular signalling pathways, particularly those pathways that rely on specific concentrations of calcium, and so may be disrupted by calcium carbonate shell dissolution.
Event(s):
Bibby_etal_08 * Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatmentExp treatBibby, Ruth
2SalinitySalBibby, RuthTetra Con 325 salinity and temperature probe
3Temperature, waterTemp°CBibby, RuthTetra Con 325 salinity and temperature probe
4Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
5pHpHBibby, RuthpH meter (Mettler Toledo InLab 413 SG)NBS scale
6pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
7Alkalinity, totalATµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
8Carbon, inorganic, dissolvedDICµmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
9Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
10Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
11Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
12Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmBibby, RuthpH meter (Mettler Toledo InLab 413 SG)
13Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
14Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
15Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
16Phagocytosed particles, number per mass proteinPhagocytosed particles#/mg proteinBibby, RuthSpectraMax microplate reader (Molecular Devices)
17Superoxyde dismutase change, number per mass proteinSOD change#/mg proteinBibby, RuthSpectraMax microplate reader (Molecular Devices)
18Blood cellsBlood cells#/mlBibby, RuthNeubauer haemocytometer
19Basophil cells, absolute numbersBASO no#Bibby, RuthNeubauer haemocytometer
20Eosinophil cells, absolute numbersEOSINO no#Bibby, RuthNeubauer haemocytometer
21Basophil cellsBASO%Bibby, Ruth
22Basophil cellsBASO%Bibby, Ruth
Size:
6124 data points

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