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Mincarelli, Luana Fiorella; Chapman, Emma C; Rotchell, Jeanette M; Turner, Alexander P; Wollenberg Valero, Katharina C (2022): Seawater carbonate chemistry and gene expression in Mytilus edulis [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.956746

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Abstract:
Plastic pollution and changes in oceanic pH are both pressing environmental issues. Little emphasis, however, has been placed on the influence of sex and gametogenesis stage when investigating the effects of such stressors. Here, we examined histology and molecular biomarkers of blue mussels Mytilus edulis exposed for 7 days to a pH 7.7 scenario (− 0.4 units) in combination with environmentally relevant concentrations (0, 0.5 and 50 µg/L) of the endocrine disrupting plasticiser di-2-ethylhexyl phthalate (DEHP). Through a factorial design, we investigated the gametogenesis cycle and sex-related expression of genes involved in pH homeostasis, stress response and oestrogen receptor-like pathways after the exposure to the two environmental stressors. As expected, we found sex-related differences in the proportion of developing, mature and spawning gonads in histological sections. Male gonads also showed higher levels of the acid–base regulator CA2, but females had a higher expression of stress response-related genes (i.e. sod, cat, hsp70). We found a significant effect of DEHP on stress response-related gene expression that was dependent on the gametogenesis stage, but there was only a trend towards downregulation of CA2 in response to pH 7.7. In addition, differences in gene expression between males and females were most pronounced in experimental conditions containing DEHP and/or acidified pH but never the control, indicating that it is important to consider sex and gametogenesis stage when studying the response of mussels to diverse stressors.
Keyword(s):
Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Gene expression (incl. proteomics); Laboratory experiment; Laboratory strains; Mollusca; Mytilus edulis; Not applicable; Organic toxins; Single species
Supplement to:
Mincarelli, Luana Fiorella; Chapman, Emma C; Rotchell, Jeanette M; Turner, Alexander P; Wollenberg Valero, Katharina C (2022): Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7. Environmental Science and Pollution Research, 30(9), 23437-23449, https://doi.org/10.1007/s11356-022-23801-3
Documentation:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-03-13.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeWollenberg Valero, Katharina CStudy
2Species, unique identificationSpecies UIDWollenberg Valero, Katharina C
3Species, unique identification (URI)Species UID (URI)Wollenberg Valero, Katharina C
4Species, unique identification (Semantic URI)Species UID (Semantic URI)Wollenberg Valero, Katharina C
5FigureFigWollenberg Valero, Katharina C
6TreatmentTreatWollenberg Valero, Katharina CpH
7TreatmentTreatWollenberg Valero, Katharina CConcentrations of DEHP
8SexSexWollenberg Valero, Katharina C
9StageStageWollenberg Valero, Katharina C
10Gene expressionGene expressionWollenberg Valero, Katharina Csod
11Gene expressionGene expressionWollenberg Valero, Katharina Ccat
12Gene expressionGene expressionWollenberg Valero, Katharina Chsp70
13Gene expressionGene expressionWollenberg Valero, Katharina CCA2
14Gene expressionGene expressionWollenberg Valero, Katharina CMeER1
15Gene expressionGene expressionWollenberg Valero, Katharina CMeER2
16SalinitySalWollenberg Valero, Katharina C
17Salinity, standard deviationSal std dev±Wollenberg Valero, Katharina C
18Temperature, waterTemp°CWollenberg Valero, Katharina C
19Temperature, water, standard deviationTemp std dev±Wollenberg Valero, Katharina C
20pHpHWollenberg Valero, Katharina CNBS scale
21pH, standard deviationpH std dev±Wollenberg Valero, Katharina CNBS scale
22Alkalinity, totalATmg/lWollenberg Valero, Katharina C
23Alkalinity, total, standard deviationAT std dev±Wollenberg Valero, Katharina C
24Alkalinity, totalATµmol/kgWollenberg Valero, Katharina C
25Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
27Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
10995 data points

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