Citation:

ab00037

Name: Seawater carbonate chemistry during experiments with Mytilus edulis, 2008
Published: 2008
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Immunology/Self-protection; Laboratory experiment; Mollusca; Mytilus edulis; North Atlantic; Single species; Temperate

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Abstract:

The effects of medium term (32 d) hypercapnia on the immune response of Mytilus edulis were investigated in mussels exposed to acidified (using CO2) sea water (pH 7.7, 7.5 or 6.7; control: pH 7.8). Levels of phagocytosis increased significantly during the exposure period, suggesting an immune response induced by the experimental set-up. However, this induced stress response was suppressed when mussels were exposed to acidified sea water. Acidified sea water did not have any significant effects on other immuno-surveillance parameters measured (superoxide anion production, total and differential cell counts). These results suggest that ocean acidification may impact the physiological condition and functionality of the haemocytes and could have a significant effect on cellular signalling pathways, particularly those pathways that rely on specific concentrations of calcium, and so may be disrupted by calcium carbonate shell dissolution.

Keyword(s):

Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Immunology/Self-protection; Laboratory experiment; Mollusca; Mytilus edulis; North Atlantic; Single species; Temperate

Project(s):

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Event(s):

Bibby_etal_08 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Bibby, Ruth
2	Salinity	Sal		Bibby, Ruth	Tetra Con 325 salinity and temperature probe
3	Temperature, water	Temp	°C	Bibby, Ruth	Tetra Con 325 salinity and temperature probe
4	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
5	pH	pH		Bibby, Ruth	pH meter (Mettler Toledo InLab 413 SG)	NBS scale
6	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
7	Alkalinity, total	AT	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
8	Carbon, inorganic, dissolved	DIC	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
9	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
10	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
11	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
12	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Bibby, Ruth	pH meter (Mettler Toledo InLab 413 SG)
13	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
14	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
15	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
16	Phagocytosed particles, number per protein mass	Phagocytosed part/prot	#/mg	Bibby, Ruth	SpectraMax microplate reader (Molecular Devices)
17	Superoxyde dismutase change, number per protein mass	SOD change/prot	#/mg	Bibby, Ruth	SpectraMax microplate reader (Molecular Devices)
18	Blood cells	Blood cells	#/ml	Bibby, Ruth	Neubauer haemocytometer
19	Basophil cells, absolute numbers	BASO no	#	Bibby, Ruth	Neubauer haemocytometer
20	Eosinophil cells, absolute numbers	EOSINO no	#	Bibby, Ruth	Neubauer haemocytometer
21	Basophil cells	BASO	%	Bibby, Ruth
22	Basophil cells	BASO	%	Bibby, Ruth

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

6124 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 Sal (Tetra Con 325 salinity and te...)	3 Temp [°C] (Tetra Con 325 salinity and te...)	4 CSC flag (Calculated using seacarb afte...)	5 pH (NBS scale, pH meter (Mettler ...)	6 pH (Total scale, Calculated using...)	7 AT [µmol/kg] (Calculated using seacarb afte...)	8 DIC [µmol/kg] (Calculated using seacarb afte...)	9 CO2 [µmol/kg] (Calculated using seacarb afte...)	10 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	11 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	12 pCO2water_SST_wet [µatm] (pH meter (Mettler Toledo InLa...)	13 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	14 Omega Arg (Calculated using seacarb afte...)	15 Omega Cal (Calculated using seacarb afte...)	16 Phagocytosed part/prot [#/mg] (SpectraMax microplate reader ...)	17 SOD change/prot [#/mg] (SpectraMax microplate reader ...)	18 Blood cells [#/ml] (Neubauer haemocytometer)	19 BASO no [#] (Neubauer haemocytometer)	20 EOSINO no [#] (Neubauer haemocytometer)	21 BASO [%]	22 BASO [%]
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.79	1946	1846.1	24.81	79.15	1742.14	665.7	662.4	1.21	1.89	106.609	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.79	1946	1846.1	24.81	79.15	1742.14	665.7	662.4	1.21	1.89	18.627	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.79	1946	1846.1	24.81	79.15	1742.14	665.7	662.4	1.21	1.89	36.950	0.002
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.79	1946	1846.1	24.81	79.15	1742.14	665.7	662.4	1.21	1.89	80.641	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	105.204	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	38.034	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	89.194	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	21.495	0.011
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	61.061	0.001
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	55.797	0.003
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	114.412	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	112.389	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	110.709	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	61.627	0.005
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	94.920	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	94.734	0.011
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	83.055	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	172.313	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	104.038	0.019
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	100.155	0.029
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	51.719	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	57.750	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	49.358	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	122.901	0.006
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	30.619	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	47.321	0.016
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	45.929	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	46.053	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	76.709	0.010
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	50.336	0.037
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	20.081	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	74.156	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	70.262	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	44.517	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	37.284	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	118.650	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	22.105	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	30.141	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	64.605	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	10.989	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	58.284	0.068
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	75.204	0.004
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	111.459	0.007
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	128.205	0.034
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	68.782	0.057
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	65.813	0.017
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	84.772	0.013
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	39.303	0.071
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	61.904	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	45.064	0.018
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	165.449	0.008
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	50.643	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	252.268	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	42.782	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	81.453	0.009
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	196.086	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	197.239	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	9.525	0.001
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	45.704	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	108.307	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	54.013	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	129.624	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	1.993	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	17.841	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	0.000	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	54.082	0.001
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	440.764	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	30.684	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	229.127	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	53.652	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	100.504	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	27.712	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	133.813	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	181.982	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	161.243	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	303.940	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	2.003	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	33.302	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	227.474	0.003
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	89.230	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	23.092	0.001
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	125.710	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	201.954	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	54.272	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	169.891	0.003
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	15.948	0.001
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	8.506	0.001
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	23.434	0.042
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	0.000	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	0.000	0.152
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	3.901	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	54.921	0.004
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	0.000	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	0.535	0.063
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	99.226	0.025
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	0.000	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	82.785	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	0.000	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	0.000	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	324.500	0.201
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	0.000	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	0.000	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	0.000	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	8.050	0.301
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	0.000	0.027
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	0.000	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	0.000	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.791
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.235
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	0.000	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	915.641	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	999.390	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	60.289	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	95.651	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	72.836	0.001
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	5.720	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	57.921	0.001
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	35.521	0.001
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	32.962	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	42.520	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	13.714	0.003
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	54.085	0.002
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	452.381	0.046
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	326.389	0.001
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	178.860	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	162.078	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	230.548	1.723
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	168.584	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	387.666	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	297.194	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	269.240	0.163
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	108.067	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	378.864	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	295.779	0.080
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	266.563	0.020
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	650.181	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	312.904	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	173.809	0.072
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	203.448	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	198.008	0.145
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	233.518	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	165.627	0.008
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	486.345	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	586.957	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	339.709	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	277.607	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	230.908	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	329.372	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	298.402	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	330.044	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	333.075	0.000
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	395.636	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	352.194	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	497.659	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	296.306	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	316.827	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	462.402	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	433.964	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	266.292	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	229.382	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	282.469	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	373.553	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	116.607	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	131.708	0.012
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	57.479	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	113.465	0.148
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	67.859	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	74.907	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	82.109	0.000
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	49.974	0.347
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01	107.555	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	62.382	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	345.319	0.006
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	167.668	1.176
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	190.094	0.079
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	262.879	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	190.785	0.320
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	216.089	0.115
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	126.971	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	210.486	0.000
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40	219.497	0.075
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	163.187	1.271
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	433.776	0.040
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	246.272	0.008
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	401.460	0.007
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	217.458	0.888
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	1770.756	0.024
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	336.107	0.493
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	141.006	0.006
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27	328.668	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	641.688	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	585.951	0.013
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	342.382	0.022
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	576.642	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	601.324	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	492.861	0.079
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	261.515	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	385.500	0.192
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84	927.077	0.000
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			5220000	110	90	55	45
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			8280000	130	70	65	35
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			3820000	129	71	64	36
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			5500000	139	61	70	30
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			2240000	138	62	69	31
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			3820000	105	95	52	48
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			7620000	98	102	49	51
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			10540000	100	100	50	50
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			18200000	127	73	64	36
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			10480000	114	86	57	43
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			6560000	102	98	51	49
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			8700000	90	110	45	55
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			6720000	105	95	52	48
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			5160000	100	100	50	50
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			5380000	131	69	66	34
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			10340000	110	90	55	45
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			6340000	116	84	58	42
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			4120000	137	63	68	32
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			6400000	108	92	54	46
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			1380000	96	104	48	52
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			5080000	114	86	57	43
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			10040000	78	122	39	61
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			6460000	118	82	59	41
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			10520000	129	71	64	36
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			4040000	131	69	66	34
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			4020000	142	58	71	29
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			10100000	124	76	62	38
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			7940000	132	68	66	34
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			5620000	146	54	73	27
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			6640000	136	64	68	32
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			2920000	78	122	39	61
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			12820000	120	80	60	40
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			2800000	97	103	48	52
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			3100000	119	81	60	40
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			1900000	108	92	54	46
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			8200000	111	89	56	44
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			2100000	115	85	58	42
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			1200000	92	108	46	54
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			16400000	112	88	56	44
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			5400000	123	77	62	38
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			7400000	108	92	54	46
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			4400000	115	85	58	42
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			9300000	113	87	56	44
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			6440000	129	71	64	36
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			6020000	95	105	48	52
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			2500000	114	86	57	43
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			3200000	117	83	58	42
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			800000	135	65	68	32
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			3300000	104	96	52	48
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			5900000	112	88	56	44
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			4900000	111	89	56	44
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			420000	117	83	58	42
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			5600000	106	94	53	47
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			3800000	134	66	67	33
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			1280000	113	87	56	44
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			3500000	106	94	53	47
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			5500000	122	78	61	39
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			19500000	159	41	80	20
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			16700000	133	67	66	34
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			19200000	127	73	64	36
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			18400000	118	82	59	41
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			16300000	132	68	66	34
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			11700000	126	74	63	37
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			1200000	142	58	71	29
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			1800000	110	90	55	45
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			4100000	114	86	57	43
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			5900000	118	82	59	41
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			4900000	107	93	54	46
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			5400000	136	64	68	32
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			2320000	127	73	64	36
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			4300000	118	82	59	41
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			3800000	124	76	62	38
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			5800000	115	85	58	42
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			7100000	129	71	64	36
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			5500000	144	56	72	28
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			7500000	137	63	68	32
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			12200000	129	71	64	36
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			4700000	150	50	75	25
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			2020000	115	85	58	42
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			1700000	123	77	62	38
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			9460000	93	107	46	54
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			8620000	88	112	44	56
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			2740000	87	113	44	56
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			9320000	100	100	50	50
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			11240000	84	116	42	58
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			4660000	92	108	46	54
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			9100000	95	105	48	52
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			5900000	126	74	63	37
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			6800000	112	88	56	44
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			2200000	144	56	72	28
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			8500000	118	82	59	41
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			2100000	119	81	60	40
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			16140000	83	117	42	58
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			19120000	101	99	50	50
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			10700000	105	95	52	48
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			1800000	98	102	49	51
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			7700000	105	95	52	48
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			1800000	95	105	48	52
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			10720000	164	36	82	18
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			6100000	111	89	56	44
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			6840000	130	70	65	35
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			3240000	119	81	60	40
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			5160000	125	75	62	38
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			7820000	115	85	58	42
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			13300000	103	97	52	48
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			8260000	98	102	49	51
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			7300000	84	116	42	58
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			7900000	112	88	56	44
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			6360000	91	109	46	54
Treatment pH=7.9 pCO2=665.7	34	15	29	7.9	7.78	1927	1832.5	24.95	76.57	1730.98	665.7	662.3	1.18	1.84			5300000	106	94	53	47
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			2100000	116	84	58	42
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			2500000	78	122	39	61
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			9700000	66	134	33	67
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			2660000	81	119	40	60
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			4740000	84	116	42	58
Treatment pH=7.7 pCO2=1160.7	34	15	29	7.7	7.58	2039	2000.9	43.53	53.11	1904.26	1160.7	1155.6	0.82	1.27			8000000	104	96	52	48
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			6600000	106	94	53	47
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			3200000	95	105	48	52
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			2700000	132	68	66	34
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			1240000	120	80	60	40
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			8300000	112	88	56	44
Treatment pH=7.4 pCO2=1435.2	34	15	29	7.4	7.28	1228	1250.4	53.83	16.49	1180.08	1435.2	1429.0	0.25	0.40			7800000	144	56	72	28
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			5600000	73	127	36	64
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			1600000	60	140	30	70
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			900000	113	87	56	44
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			6200000	102	98	51	49
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			2000000	80	120	40	60
Treatment pH=6.5 pCO2=3316.2	34	15	29	6.5	6.38	346	468.3	124.39	0.60	343.31	3316.2	3302.1	0.01	0.01			1600000	109	91	54	46