Citation:

ame01820

Name: Seawater carbonate chemistry and growth and chlorophyll, photochemical parameters, carbon fixation of diatom Phaeodactylum tricornutum
Published: 2017
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Single species

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Abstract:

Elevated CO2 is leading to a decrease in pH in marine environments (ocean acidification [OA]), altering marine carbonate chemistry. OA can influence the metabolism of many marine organisms; however, no consensus has been reached on its effects on algal photosynthetic carbon fixation and primary production. Here, we found that when the diatom Phaeodactylum tricornutum was grown under different pCO2 levels, it showed different responses to elevated pCO2 levels under growth-limiting (20 µmol photons/m**2/s, LL) compared with growth-saturating (200 µmol photons/m**2/s, HL) light levels. With pCO2 increased up to 950 µatm, growth rates and primary productivity increased, but in the HL cells, these parameters decreased significantly at higher concentrations up to 5000 µatm, while no difference in growth was observed with pCO2 for the LL cells. Elevated CO2 concentrations reduced the size of the intracellular dissolved inorganic carbon (DIC) pool by 81% and 60% under the LL and HL levels, respectively, with the corresponding photosynthetic affinity for DIC decreasing by 48% and 55%. Little photoinhibition was observed across all treatments. These results suggest that the decreased growth rates under higher CO2 levels in the HL cells were most likely due to acid stress. Low energy demand of growth and energy saving from the down-regulation of the CO2 concentrating mechanisms (CCM) minimized the effects of acid stress on the growth of the LL cells. These findings imply that OA treatment, except for down-regulating CCM, caused stress on the diatom, reflected in diminished C assimilation and growth rates.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Single species

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-04-25.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Gao, Kunshan		study
2	Species	Species		Gao, Kunshan
3	Registration number of species	Reg spec no		Gao, Kunshan
4	Uniform resource locator/link to reference	URL ref		Gao, Kunshan		WoRMS Aphia ID
5	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Gao, Kunshan
6	Treatment	Treat		Gao, Kunshan
7	Identification	ID		Gao, Kunshan		sample
8	Growth rate	µ	1/day	Gao, Kunshan
9	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Gao, Kunshan
10	Time in seconds	Time	s	Gao, Kunshan
11	Effective quantum yield	Y		Gao, Kunshan		under 76 μmol photons/m**2/s
12	Effective quantum yield	Y		Gao, Kunshan		under 226 μmol photons/m**2/s
13	Light saturation point	Ik	µmol/m²/s	Gao, Kunshan
14	Maximal electron transport rate, relative	rETR max		Gao, Kunshan
15	Light capturing capacity	alpha		Gao, Kunshan
16	Cumulative carbon fixation per cell	Cum C fix/cell	pg/#	Gao, Kunshan		per 12h
17	Cumulative carbon fixation per cell	Cum C fix/cell	pg/#	Gao, Kunshan		per 24h
18	Initial slope of photosynthesis/dissolved inorganic carbon	Initial slope PN/DIC		Gao, Kunshan
19	Light saturated maximum photosynthetic rate per cell	Vmax/cell	pg/#/h	Gao, Kunshan
20	Carbon, inorganic, dissolved, intracellular pool	DIC in pool	µmol/l	Gao, Kunshan
21	Factor	Factor		Gao, Kunshan		accumulation for CO2
22	Temperature, water	Temp	°C	Gao, Kunshan
23	Salinity	Sal		Gao, Kunshan
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Gao, Kunshan
25	pH	pH		Gao, Kunshan		NBS scale
26	Alkalinity, total	AT	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
27	Carbon, inorganic, dissolved	DIC	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
28	Bicarbonate ion	[HCO3]-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
29	Carbonate ion	[CO3]2-	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
30	Carbon dioxide	CO2	µmol/kg	Gao, Kunshan	Calculated using CO2SYS
31	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
33	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

6177 data points

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