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Arnosti, Carol; Grossart, Hans-Peter; Mühling, M; Joint, Ian; Passow, Uta (2011): PEECE II mesocosm experiment: Dynamics of extracellular enzyme activities in seawater under changed atmospheric pCO2, 2011. doi:10.1594/PANGAEA.778190,
Supplement to: Arnosti, C et al. (2011): Dynamics of extracellular enzyme activities in seawater under changed atmospheric pCO2: a mesocosm investigation. Aquatic Microbial Ecology, 64(3), 285-298, doi:10.3354/ame01522

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Abstract:
As part of the PeECE II mesocosm project, we investigated the effects of pCO2 levels on the initial step of heterotrophic carbon cycling in the surface ocean. The activities of microbial extracellular enzymes hydrolyzing 4 polysaccharides were measured during the development of a natural phytoplankton bloom under pCO2 conditions representing glacial (190 µatm) and future (750 µatm) atmospheric pCO2. We observed that (1) chondroitin hydrolysis was variable throughout the pre-, early- and late-bloom phases, (2) fucoidanase activity was measurable only in the glacial mesocosm as the bloom developed, (3) laminarinase activity was low and constant, and (4) xylanase activity declined as the bloom progressed. Concurrent measurements of microbial community composition, using denaturing-gradient gel electrophoresis (DGGE), showed that the 2 mesocosms diverged temporally, and from one another, especially in the late-bloom phase. Enzyme activities correlated with bloom phase and pCO2, suggesting functional as well as compositional changes in microbial communities in the different pCO2 environments. These changes, however, may be a response to temporal changes in the development of phytoplankton communities that differed with the pCO2 environment. We hypothesize that the phytoplankton communities produced dissolved organic carbon (DOC) differing in composition, a hypothesis supported by changing amino acid composition of the DOC, and that enzyme activities responded to changes in substrates. Enzyme activities observed under different pCO2 conditions likely reflect both genetic and population-level responses to changes occurring among multiple components of the microbial loop.
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1IdentificationIDArnosti, Carol
2Experimental treatmentExp trtmArnosti, Carol
3Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmArnosti, Carol
4Experiment dayExp daydayArnosti, Carol
5Time, incubationT incubationdayArnosti, Carol
6Cell densityCells#/mlArnosti, Carol
7Cell density, standard deviationCells std dev±Arnosti, Carol
8Proportion of total bacteria attached to particlesBact prop particles%Arnosti, Carol
9Bacteria, production as carbonBCPng/l/hArnosti, Carol14C-leucine incorporation
10Bacterial cell multiplicationBCM#/ml/hArnosti, CarolThymidine incorporation
11Sample IDSample IDArnosti, Carol
12Chondroitin sulfate hydrolysisCSHnmol monomer/l/ hArnosti, Carolsee reference(s)
13Laminarin hydrolysisLHnmol monomer/l/ hArnosti, Carolsee reference(s)
14Xylan hydrolysisXHnmol monomer/l/ hArnosti, Carolsee reference(s)
15Fucoidan hydrolysisFHnmol monomer/l/ hArnosti, Carolsee reference(s)
16SalinitySalArnosti, Carol
17Temperature, waterTemp°CArnosti, Carol
18Carbon, inorganic, dissolvedDICµmol/kgArnosti, CarolMeasured
19Alkalinity, totalATµmol/kgArnosti, CarolMeasured
20Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
21pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
22Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
23Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
24Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
25Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
26Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
27Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
28Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
Size:
664 data points

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