Data Description

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Citation:
Arnosti, C et al. (2011): PEECE II mesocosm experiment: Dynamics of extracellular enzyme activities in seawater under changed atmospheric pCO2, 2011. doi:10.1594/PANGAEA.778190,
Supplement to: Arnosti, Carol; Grossart, Hans-Peter; Mühling, M; Joint, Ian; Passow, Uta (2011): Dynamics of extracellular enzyme activities in seawater under changed atmospheric pCO2: a mesocosm investigation. Aquatic Microbial Ecology, 64(3), 285-298, doi:10.3354/ame01522
Abstract:
As part of the PeECE II mesocosm project, we investigated the effects of pCO2 levels on the initial step of heterotrophic carbon cycling in the surface ocean. The activities of microbial extracellular enzymes hydrolyzing 4 polysaccharides were measured during the development of a natural phytoplankton bloom under pCO2 conditions representing glacial (190 µatm) and future (750 µatm) atmospheric pCO2. We observed that (1) chondroitin hydrolysis was variable throughout the pre-, early- and late-bloom phases, (2) fucoidanase activity was measurable only in the glacial mesocosm as the bloom developed, (3) laminarinase activity was low and constant, and (4) xylanase activity declined as the bloom progressed. Concurrent measurements of microbial community composition, using denaturing-gradient gel electrophoresis (DGGE), showed that the 2 mesocosms diverged temporally, and from one another, especially in the late-bloom phase. Enzyme activities correlated with bloom phase and pCO2, suggesting functional as well as compositional changes in microbial communities in the different pCO2 environments. These changes, however, may be a response to temporal changes in the development of phytoplankton communities that differed with the pCO2 environment. We hypothesize that the phytoplankton communities produced dissolved organic carbon (DOC) differing in composition, a hypothesis supported by changing amino acid composition of the DOC, and that enzyme activities responded to changes in substrates. Enzyme activities observed under different pCO2 conditions likely reflect both genetic and population-level responses to changes occurring among multiple components of the microbial loop.
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Identification *IDArnosti, Carol *
2Experimental treatment *Exp trtmArnosti, Carol *
3Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmArnosti, Carol *
4Experiment day *Exp daydayArnosti, Carol *
5Time, incubation *T incubationdayArnosti, Carol *
6Cell density *Cells#/mlArnosti, Carol *
7Cell density, standard deviation *Cells std dev±Arnosti, Carol *
8Proportion of total bacteria attached to particles *Bact prop particles%Arnosti, Carol *
9Bacteria, production as carbon *BCPng/l/hArnosti, Carol *14C-leucine incorporation *
10Bacterial cell multiplication *BCM#/ml/hArnosti, Carol *Thymidine incorporation *
11Sample ID *Sample IDArnosti, Carol *
12Chondroitin sulfate hydrolysis *CSHnmol monomer/l/ hArnosti, Carol *see reference(s) *
13Laminarin hydrolysis *LHnmol monomer/l/ hArnosti, Carol *see reference(s) *
14Xylan hydrolysis *XHnmol monomer/l/ hArnosti, Carol *see reference(s) *
15Fucoidan hydrolysis *FHnmol monomer/l/ hArnosti, Carol *see reference(s) *
16Salinity *SalArnosti, Carol *
17Temperature, water *Temp°CArnosti, Carol *
18Carbon, inorganic, dissolved *DICµmol/kgArnosti, Carol *Measured *
19Alkalinity, total *ATµmol/kgArnosti, Carol *Measured *
20Carbonate system computation flag *CSC flagNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
21pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Total scale
22Carbon dioxide *CO2µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
23Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
24Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
25Bicarbonate ion *[HCO3]-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
26Carbonate ion *[CO3]2-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
27Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
28Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
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664 data points

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