Citation:

ame01522

Name: PEECE II mesocosm experiment: Dynamics of extracellular enzyme activities in seawater under changed atmospheric pCO2, 2011
Published: 2011
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Coast and continental shelf; Field experiment; Mesocosm or benthocosm; North Atlantic; Other metabolic rates; Pelagos; Temperate

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Abstract:

As part of the PeECE II mesocosm project, we investigated the effects of pCO2 levels on the initial step of heterotrophic carbon cycling in the surface ocean. The activities of microbial extracellular enzymes hydrolyzing 4 polysaccharides were measured during the development of a natural phytoplankton bloom under pCO2 conditions representing glacial (190 µatm) and future (750 µatm) atmospheric pCO2. We observed that (1) chondroitin hydrolysis was variable throughout the pre-, early- and late-bloom phases, (2) fucoidanase activity was measurable only in the glacial mesocosm as the bloom developed, (3) laminarinase activity was low and constant, and (4) xylanase activity declined as the bloom progressed. Concurrent measurements of microbial community composition, using denaturing-gradient gel electrophoresis (DGGE), showed that the 2 mesocosms diverged temporally, and from one another, especially in the late-bloom phase. Enzyme activities correlated with bloom phase and pCO2, suggesting functional as well as compositional changes in microbial communities in the different pCO2 environments. These changes, however, may be a response to temporal changes in the development of phytoplankton communities that differed with the pCO2 environment. We hypothesize that the phytoplankton communities produced dissolved organic carbon (DOC) differing in composition, a hypothesis supported by changing amino acid composition of the DOC, and that enzyme activities responded to changes in substrates. Enzyme activities observed under different pCO2 conditions likely reflect both genetic and population-level responses to changes occurring among multiple components of the microbial loop.

Keyword(s):

Biomass/Abundance/Elemental composition; Coast and continental shelf; Field experiment; Mesocosm or benthocosm; North Atlantic; Other metabolic rates; Pelagos; Temperate

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Sixth Framework Programme (FP6), grant/award no. 511106 : European network of excellence for Ocean Ecosystems Analysis

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Identification	ID		Arnosti, Carol
2	Experimental treatment	Exp treat		Arnosti, Carol
3	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Arnosti, Carol
4	Experiment day	Exp day	day	Arnosti, Carol
5	Time, incubation	T incubation	day	Arnosti, Carol
6	Cell density	Cells	#/ml	Arnosti, Carol
7	Cell density, standard deviation	Cells std dev	±	Arnosti, Carol
8	Proportion of total bacteria attached to particles	Bact prop particles	%	Arnosti, Carol
9	Bacteria, production as carbon	BCP	ng/l/h	Arnosti, Carol	14C-leucine incorporation
10	Bacterial cell multiplication	BCM	#/ml/h	Arnosti, Carol	Thymidine incorporation
11	Sample ID	Sample ID		Arnosti, Carol
12	Chondroitin sulfate hydrolysis	CSH	nmol monomer/l/ h	Arnosti, Carol	see reference(s)
13	Laminarin hydrolysis	LH	nmol monomer/l/ h	Arnosti, Carol	see reference(s)
14	Xylan hydrolysis	XH	nmol monomer/l/ h	Arnosti, Carol	see reference(s)
15	Fucoidan hydrolysis	FH	nmol monomer/l/ h	Arnosti, Carol	see reference(s)
16	Salinity	Sal		Arnosti, Carol
17	Temperature, water	Temp	°C	Arnosti, Carol
18	Carbon, inorganic, dissolved	DIC	µmol/kg	Arnosti, Carol	Measured
19	Alkalinity, total	AT	µmol/kg	Arnosti, Carol	Measured
20	Carbonate system computation flag	CSC flag		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
21	pH	pH		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)	Total scale
22	Carbon dioxide	CO2	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
23	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
24	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
25	Bicarbonate ion	[HCO3]-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
26	Carbonate ion	[CO3]2-	µmol/kg	Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
27	Aragonite saturation state	Omega Arg		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)
28	Calcite saturation state	Omega Cal		Nisumaa, Anne-Marin	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

664 data points

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