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Strobel, Anneli; Leo, Elettra; Pörtner, Hans-Otto; Mark, Felix Christopher (2014): Experiment: Elevated temperature and PCO2 affect enzyme activities in differentially oxidative tissues of Notothenia rossii. PANGAEA, https://doi.org/10.1594/PANGAEA.829831, Supplement to: Strobel, A et al. (2013): Elevated temperature and PCO2 shift metabolic pathways in differentially oxidative tissues of Notothenia rossii. Comparative Biochemistry and Physiology Part B: Biochemistry & Molecular Biology, 166(1), 48-57, https://doi.org/10.1016/j.cbpb.2013.06.006

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Abstract:
Mitochondrial plasticity plays a central role in setting the capacity for acclimation of aerobic metabolism in ectotherms in response to environmental changes. We still lack a clear picture if and to what extent the energy metabolism and mitochondrial enzymes of Antarctic fish can compensate for changing temperatures or PCO2 and whether capacities for compensation differ between tissues. We therefore measured activities of key mitochondrial enzymes (citrate synthase (CS), cytochrome c oxidase (COX)) from heart, red muscle, white muscle and liver in the Antarctic fish Notothenia rossii after warm- (7 °C) and hypercapnia- (0.2 kPa CO2) acclimation vs. control conditions (1 °C, 0.04 kPa CO2). In heart, enzymes showed elevated activities after cold-hypercapnia acclimation, and a warm-acclimation-induced upward shift in thermal optima. The strongest increase in enzyme activities in response to hypercapnia occurred in red muscle. In white muscle, enzyme activities were temperature-compensated. CS activity in liver decreased after warm-normocapnia acclimation (temperature-compensation), while COX activities were lower after cold- and warm-hypercapnia exposure, but increased after warm-normocapnia acclimation. In conclusion, warm-acclimated N. rossii display low thermal compensation in response to rising energy demand in highly aerobic tissues, such as heart and red muscle. Chronic environmental hypercapnia elicits increased enzyme activities in these tissues, possibly to compensate for an elevated energy demand for acid-base regulation or a compromised mitochondrial metabolism, that is predicted to occur in response to hypercapnia exposure. This might be supported by enhanced metabolisation of liver energy stores. These patterns reflect a limited capacity of N. rossii to reorganise energy metabolism in response to rising temperature and PCO2.
Further details:
Strobel, Anneli; Leo, Elettra; Pörtner, Hans-Otto; Mark, Felix Christopher (2013): Seawater carbonate chemistry, citrate synthase (CS), cytochrome c oxidase (COX) of the Antarctic fish Notothenia rossii in a laboratory experiment. PANGAEA, https://doi.org/10.1594/PANGAEA.831182
Coverage:
Latitude: -62.233330 * Longitude: -58.666660
Minimum Elevation: -35.0 m * Maximum Elevation: -35.0 m
Event(s):
PotterCove * Latitude: -62.233330 * Longitude: -58.666660 * Elevation: -35.0 m * Location: Potter Cove, King George Island, Antarctic Peninsula * Campaign: Jubany_Dallmann * Basis: Carlini/Jubany Station * Method/Device: Multiple investigations (MULT)
Comment:
Notothenia rossii acclimated to higher temperature and PCO2; Citrate synthase (CS) and cytochrome c oxidase (COX) activities are given in nmol per minute and mg protein (CS/protein; COX/protein) or mg tissue fresh mass (CS/MW; COX/MW) at the assay temperatures of 0, 6, 9, 12 °C in heart, liver, red muscle & white muscle tissue of all acclimated animals and the control.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1IdentificationIDStrobel, AnneliNo. of experimental animal
2SpeciesSpeciesStrobel, Anneli
3Temperature, technicalT tech°CStrobel, AnneliAcclimation temperature
4Carbon dioxide, partial pressurepCO2ppmvStrobel, AnneliAcclimation PCO2
5Temperature, technicalT tech°CStrobel, AnneliEnzyme assay temperature
6Citrate synthase activity per proteinCS act/proteinnmol/min/mgStrobel, AnneliTissue: heart
7Cytochrome c oxidase activity per proteinCOX act/proteinnmol/min/mgStrobel, AnneliTissue: heart
8Citrate synthase activity per proteinCS act/proteinnmol/min/mgStrobel, AnneliTissue: liver
9Cytochrome c oxidase activity per proteinCOX act/proteinnmol/min/mgStrobel, AnneliTissue: liver
10Citrate synthase activity per proteinCS act/proteinnmol/min/mgStrobel, AnneliTissue: red muscle
11Cytochrome c oxidase activity per proteinCOX act/proteinnmol/min/mgStrobel, AnneliTissue: red muscle
12Citrate synthase activity per proteinCS act/proteinnmol/min/mgStrobel, AnneliTissue: white muscle
13Cytochrome c oxidase activity per proteinCOX act/proteinnmol/min/mgStrobel, AnneliTissue: white muscle
14Citrate synthase activity per fresh massCS act/fmnmol/min/mgStrobel, AnneliTissue: heart
15Cytochrome c oxidase activity, per fresh massCOX act/fmnmol/min/mgStrobel, AnneliTissue: heart
16Citrate synthase activity per fresh massCS act/fmnmol/min/mgStrobel, AnneliTissue: liver
17Cytochrome c oxidase activity, per fresh massCOX act/fmnmol/min/mgStrobel, AnneliTissue: liver
18Citrate synthase activity per fresh massCS act/fmnmol/min/mgStrobel, AnneliTissue: red muscle
19Cytochrome c oxidase activity, per fresh massCOX act/fmnmol/min/mgStrobel, AnneliTissue: red muscle
20Citrate synthase activity per fresh massCS act/fmnmol/min/mgStrobel, AnneliTissue: white muscle
21Cytochrome c oxidase activity, per fresh massCOX act/fmnmol/min/mgStrobel, AnneliTissue: white muscle
Size:
2003 data points

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