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Trimborn, Scarlett; Wolf-Gladrow, Dieter A; Richter, Klaus-Uwe; Rost, Bjoern (2010): Seawater carbonate chemistry and biological processes during experiments with four species of marine diatoms. doi:10.1594/PANGAEA.733948,
Supplement to: Trimborn, S et al. (2009): The effect of pCO2 on carbon acquisition and intracellular assimilation in four marine diatoms. Journal of Experimental Marine Biology and Ecology, 376(1), 26-36, doi:10.1016/j.jembe.2009.05.017

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Abstract:
The effect of pCO2 on carbon acquisition and intracellular assimilation was investigated in the three bloom-forming diatom species, Eucampia zodiacus (Ehrenberg), Skeletonema costatum (Greville) Cleve, Thalassionema nitzschioides (Grunow) Mereschkowsky and the non-bloom-forming Thalassiosira pseudonana (Hust.) Hasle and Heimdal. In vivo activities of carbonic anhydrase (CA), photosynthetic O2 evolution, CO2 and HCO3? uptake rates were measured by membrane-inlet mass spectrometry (MIMS) in cells acclimated to pCO2 levels of 370 and 800 ?atm. To investigate whether the cells operate a C4-like pathway, activities of ribulose-1,5-bisphosphate carboxylase (RubisCO) and phosphoenolpyruvate carboxylase (PEPC) were measured at the mentioned pCO2 levels and a lower pCO2 level of 50 ?atm. In the bloom-forming species, extracellular CA activities strongly increased with decreasing CO2 supply while constantly low activities were obtained for T. pseudonana. Half-saturation concentrations (K1/2) for photosynthetic O2 evolution decreased with decreasing CO2 supply in the two bloom-forming species S. costatum and T. nitzschioides, but not in T. pseudonana and E. zodiacus. With the exception of S. costatum, maximum rates (Vmax) of photosynthesis remained constant in all investigated diatom species. Independent of the pCO2 level, PEPC activities were significantly lower than those for RubisCO, averaging generally less than 3%. All examined diatom species operate highly efficient CCMs under ambient and high pCO2, but differ strongly in the degree of regulation of individual components of the CCM such as Ci uptake kinetics and extracellular CA activities. The present data do not suggest C4 metabolism in the investigated species.
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatmentExp trtmTrimborn, Scarlett
2SpeciesSpeciesTrimborn, Scarlett
3SalinitySalTrimborn, Scarlett
4Temperature, waterTemp°CTrimborn, Scarlett
5Light:Dark cycleL:Dhh:hhTrimborn, Scarlett
6Radiation, photosynthetically activePARµE/m2/sTrimborn, Scarlett
7pHpHTrimborn, ScarlettpH meter, WTW, pH 3000NBS scale
8pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
9Alkalinity, totalATµmol/kgTrimborn, ScarlettAlkalinity, Gran titration (Gran, 1950)
10Carbon, inorganic, dissolvedDICµmol/kgTrimborn, ScarlettCalculated using CO2SYS
11Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmTrimborn, ScarlettCalculated using CO2SYS
12Carbon dioxideCO2µmol/kgTrimborn, ScarlettCalculated using CO2SYS
13Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
14Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
15Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
16Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
17Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
18Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
19Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
20Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
21Extracellular carbonic anhydrase activityeCA activityU/µg Chl aTrimborn, ScarlettMeasured by loss of 18O (Silverman, 1982)
22Intracellular carbonic anhydrase activity per chlorophyll aCA in activitydelta/µgTrimborn, ScarlettMeasured by loss of 18O (Silverman, 1982)
23Bicarbonate uptake[HCO3]- upt%Trimborn, ScarlettCalculated
24Isotopic fractionation, during photosynthisepsilon pper milTrimborn, ScarlettCalculated after Freeman & Hayes (1992)
25Bicarbonate uptake[HCO3]- upt%Trimborn, Scarlettsee reference(s)
Size:
1263 data points

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