Data Description

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Citation:
Wu, Y et al. (2010): Seawater carbonate chemistry and diatom Phaeodactylum tricornutum (CCMA 106) biological processes during experiments, 2010. doi:10.1594/PANGAEA.758214,
Supplement to: Wu, Yaping; Gao, Kunshan; Riebesell, Ulf (2010): CO2-induced seawater acidification affects physiological performance of the marine diatom Phaeodactylum tricornutum. Biogeosciences, 7(9), 2915-2923, doi:10.5194/bg-7-2915-2010
Abstract:
CO2/pH perturbation experiments were carried out under two different pCO2 levels (39.3 and 101.3 Pa) to evaluate effects of CO2-induced ocean acidification on the marine diatom Phaeodactylum tricornutum. After acclimation (>20 generations) to ambient and elevated CO2 conditions (with corresponding pH values of 8.15 and 7.80, respectively), growth and photosynthetic carbon fixation rates of high CO2 grown cells were enhanced by 5% and 12%, respectively, and dark respiration stimulated by 34% compared to cells grown at ambient CO2. The half saturation constant (Km) for carbon fixation (dissolved inorganic carbon, DIC) increased by 20% under the low pH and high CO2 condition, reflecting a decreased affinity for HCO3- or/and CO2 and down-regulated carbon concentrating mechanism (CCM). In the high CO2 grown cells, the electron transport rate from photosystem II (PSII) was photoinhibited to a greater extent at high levels of photosynthetically active radiation, while non-photochemical quenching was reduced compared to low CO2 grown cells. This was probably due to the down-regulation of CCM, which serves as a sink for excessive energy. The balance between these positive and negative effects on diatom productivity will be a key factor in determining the net effect of rising atmospheric CO2 on ocean primary production.
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Experimental treatment *Exp trtmWu, Yaping *
2Comment *CommentWu, Yaping *
3Salinity *SalWu, Yaping *Measured *
4Temperature, water *Temp°CWu, Yaping *Measured *
5Carbon dioxide, partial pressure *pCO2PaWu, Yaping *Infrared CO2/H2O gas analyzer, non-dispersive, LI-COR type LI-7000 *
6Carbon dioxide, partial pressure, standard deviation *pCO2 std dev±Wu, Yaping *
7pH *pHWu, Yaping *pH meter (Benchtop pH510, OAKTON) *NBS scale
8pH, standard deviation *pH std dev±Wu, Yaping *
9Carbon, inorganic, dissolved *DICµmol/kgWu, Yaping *DIC analyzer (AS-C3, Apollo Scitech) *
10Carbon, inorganic, dissolved, standard deviation *DIC std dev±Wu, Yaping *
11Bicarbonate ion *[HCO3]-µmol/kgWu, Yaping *Calculated using CO2SYS *
12Bicarbonate ion, standard deviation *[HCO3]- std dev±Wu, Yaping *
13Carbonate ion *[CO3]2-µmol/kgWu, Yaping *Calculated using CO2SYS *
14Carbonate ion, standard deviation *[CO3]2- std dev±Wu, Yaping *
15Carbon dioxide *CO2µmol/kgWu, Yaping *Calculated using CO2SYS *
16Carbon dioxide, standard deviation *CO2 std dev±Wu, Yaping *
17Alkalinity, total *ATµmol/kgWu, Yaping *Calculated using CO2SYS *
18Alkalinity, total, standard deviation *AT std dev±Wu, Yaping *
19Photochemical efficiency *delta F/Fmarbitrary unitsWu, Yaping *Calculated, see reference(s) *
20Photochemical efficiency, standard deviation *delta F/Fm std dev±Wu, Yaping *
21Maximum potential photosynthesis *PmaxWu, Yaping *see reference(s) *
22Maximum potential photosynthesis, standard deviation *Pmax std dev±Wu, Yaping *
23Light saturation point *IkWu, Yaping *see reference(s) *
24Light saturation point, standard deviation *Ik std dev±Wu, Yaping *
25Chlorophyll a per cell *Chl apg/#Wu, Yaping *Spectrophotometry *
26Chlorophyll a, standard deviation *Chl a std dev±Wu, Yaping *
27Growth rate *µ#/dayWu, Yaping *Calculated *
28Growth rate, standard deviation *µ std dev±Wu, Yaping *
29Photosynthetic carbon fixation rate *C fixµmol/µg/hWu, Yaping *Liquid scintillation counter (Tri-Carb 2800TR, Perkin-Elmer) *
30Photosynthetic carbon fixation rate, standard deviation *C fix std dev±Wu, Yaping *
31Respiration rate, oxygen *Resp O2µmol/mg/hWu, Yaping *Clark type oxygen electrode (5300A, YSI) *Dark
32Respiration rate, oxygen, standard deviation *Resp O2 std dev±Wu, Yaping *Calculated *
33Carbonate system computation flag *CSC flagNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
34pH *pHNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *Total scale
35Carbon dioxide *CO2µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
36Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
37Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
38Bicarbonate ion *[HCO3]-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
39Carbonate ion *[CO3]2-µmol/kgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
40Aragonite saturation state *Omega ArgNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
41Calcite saturation state *Omega CalNisumaa, Anne-Marin *Calculated using seacarb after Nisumaa et al. (2010) *
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