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Wu, YaPing; Gao, Kunshan; Riebesell, Ulf (2010): Seawater carbonate chemistry and diatom Phaeodactylum tricornutum (CCMA 106) biological processes during experiments, 2010 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.758214, Supplement to: Wu, YP et al. (2010): CO2-induced seawater acidification affects physiological performance of the marine diatom Phaeodactylum tricornutum. Biogeosciences, 7(9), 2915-2923, https://doi.org/10.5194/bg-7-2915-2010

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Published: 2010 (exact date unknown) • DOI registered: 2011-04-08

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Abstract:
CO2/pH perturbation experiments were carried out under two different pCO2 levels (39.3 and 101.3 Pa) to evaluate effects of CO2-induced ocean acidification on the marine diatom Phaeodactylum tricornutum. After acclimation (>20 generations) to ambient and elevated CO2 conditions (with corresponding pH values of 8.15 and 7.80, respectively), growth and photosynthetic carbon fixation rates of high CO2 grown cells were enhanced by 5% and 12%, respectively, and dark respiration stimulated by 34% compared to cells grown at ambient CO2. The half saturation constant (Km) for carbon fixation (dissolved inorganic carbon, DIC) increased by 20% under the low pH and high CO2 condition, reflecting a decreased affinity for HCO3- or/and CO2 and down-regulated carbon concentrating mechanism (CCM). In the high CO2 grown cells, the electron transport rate from photosystem II (PSII) was photoinhibited to a greater extent at high levels of photosynthetically active radiation, while non-photochemical quenching was reduced compared to low CO2 grown cells. This was probably due to the down-regulation of CCM, which serves as a sink for excessive energy. The balance between these positive and negative effects on diatom productivity will be a key factor in determining the net effect of rising atmospheric CO2 on ocean primary production.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species
Project(s):
European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS)
European Project on Ocean Acidification (EPOCA)
Ocean Acidification International Coordination Centre (OA-ICC)
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Experimental treatmentExp treatWu, YaPing
2CommentCommentWu, YaPing
3SalinitySalWu, YaPingMeasured
4Temperature, waterTemp°CWu, YaPingMeasured
5Carbon dioxide, partial pressurepCO2PaWu, YaPingInfrared CO2/H2O gas analyzer, non-dispersive, LI-COR type LI-7000
6Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Wu, YaPing
7pH, NBS scalepH NBSWu, YaPingpH meter (Benchtop pH510, OAKTON)NBS scale
8pH, standard deviationpH std dev±Wu, YaPing
9Carbon, inorganic, dissolvedDICµmol/kgWu, YaPingDIC analyzer (AS-C3, Apollo Scitech)
10Carbon, inorganic, dissolved, standard deviationDIC std dev±Wu, YaPing
11Bicarbonate ion[HCO3]-µmol/kgWu, YaPingCalculated using CO2SYS
12Bicarbonate ion, standard deviation[HCO3]- std dev±Wu, YaPing
13Carbonate ion[CO3]2-µmol/kgWu, YaPingCalculated using CO2SYS
14Carbonate ion, standard deviation[CO3]2- std dev±Wu, YaPing
15Carbon dioxideCO2µmol/kgWu, YaPingCalculated using CO2SYS
16Carbon dioxide, standard deviationCO2 std dev±Wu, YaPing
17Alkalinity, totalATµmol/kgWu, YaPingCalculated using CO2SYS
18Alkalinity, total, standard deviationAT std dev±Wu, YaPing
19Photochemical efficiencydelta F/Fmarbitrary unitsWu, YaPingCalculated, see reference(s)
20Photochemical efficiency, standard deviationdelta F/Fm std dev±Wu, YaPing
21Maximum potential photosynthesisPmaxWu, YaPingsee reference(s)
22Maximum potential photosynthesis, standard deviationPmax std dev±Wu, YaPing
23Light saturation pointIkWu, YaPingsee reference(s)
24Light saturation point, standard deviationIk std dev±Wu, YaPing
25Chlorophyll a per cellChl a/cellpg/#Wu, YaPingSpectrophotometry
26Chlorophyll a, standard deviationChl a std dev±Wu, YaPing
27Growth rateµ#/dayWu, YaPingCalculated
28Growth rate, standard deviationµ std dev±Wu, YaPing
29Photosynthetic carbon fixation rateC fixµmol/µg/hWu, YaPingLiquid scintillation counter (Tri-Carb 2800TR, Perkin-Elmer)
30Photosynthetic carbon fixation rate, standard deviationC fix std dev±Wu, YaPing
31Respiration rate, oxygenResp O2µmol/mg/hWu, YaPingClark type oxygen electrode (5300A, YSI)Dark
32Respiration rate, oxygen, standard deviationResp O2 std dev±Wu, YaPingCalculated
33Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
34pH, total scalepHTNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
35Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
36Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
37Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
38Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
39Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
40Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
41Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
152 data points

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