Wu, YaPing; Gao, Kunshan; Riebesell, Ulf (2010): Seawater carbonate chemistry and diatom Phaeodactylum tricornutum (CCMA 106) biological processes during experiments, 2010. PANGAEA, https://doi.org/10.1594/PANGAEA.758214, Supplement to: Wu, YP et al. (2010): CO2-induced seawater acidification affects physiological performance of the marine diatom Phaeodactylum tricornutum. Biogeosciences, 7(9), 2915-2923, https://doi.org/10.5194/bg-7-2915-2010
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Abstract:
CO2/pH perturbation experiments were carried out under two different pCO2 levels (39.3 and 101.3 Pa) to evaluate effects of CO2-induced ocean acidification on the marine diatom Phaeodactylum tricornutum. After acclimation (>20 generations) to ambient and elevated CO2 conditions (with corresponding pH values of 8.15 and 7.80, respectively), growth and photosynthetic carbon fixation rates of high CO2 grown cells were enhanced by 5% and 12%, respectively, and dark respiration stimulated by 34% compared to cells grown at ambient CO2. The half saturation constant (Km) for carbon fixation (dissolved inorganic carbon, DIC) increased by 20% under the low pH and high CO2 condition, reflecting a decreased affinity for HCO3- or/and CO2 and down-regulated carbon concentrating mechanism (CCM). In the high CO2 grown cells, the electron transport rate from photosystem II (PSII) was photoinhibited to a greater extent at high levels of photosynthetically active radiation, while non-photochemical quenching was reduced compared to low CO2 grown cells. This was probably due to the down-regulation of CCM, which serves as a sink for excessive energy. The balance between these positive and negative effects on diatom productivity will be a key factor in determining the net effect of rising atmospheric CO2 on ocean primary production.
Keyword(s):
Project(s):
Funding:
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
152 data points
Data
1 Exp treat | 2 Comment | 3 Sal (Measured) | 4 Temp [°C] (Measured) | 5 pCO2 [Pa] (Infrared CO2/H2O gas analyzer...) | 6 pCO2 std dev [±] | 7 pH (NBS scale, pH meter (Benchtop...) | 8 pH std dev [±] | 9 DIC [µmol/kg] (DIC analyzer (AS-C3, Apollo S...) | 10 DIC std dev [±] | 11 [HCO3]- [µmol/kg] (Calculated using CO2SYS) | 12 [HCO3]- std dev [±] | 13 [CO3]2- [µmol/kg] (Calculated using CO2SYS) | 14 [CO3]2- std dev [±] | 15 CO2 [µmol/kg] (Calculated using CO2SYS) | 16 CO2 std dev [±] | 17 AT [µmol/kg] (Calculated using CO2SYS) | 18 AT std dev [±] | 19 delta F/Fm [arbitrary units] (Calculated, see reference(s)) | 20 delta F/Fm std dev [±] | 21 Pmax (see reference(s)) | 22 Pmax std dev [±] | 23 Ik (see reference(s)) | 24 Ik std dev [±] | 25 Chl a/cell [pg/#] (Spectrophotometry) | 26 Chl a std dev [±] | 27 µ [#/day] (Calculated) | 28 µ std dev [±] | 29 C fix [µmol/µg/h] (Liquid scintillation counter ...) | 30 C fix std dev [±] | 31 Resp O2 [µmol/mg/h] (Dark, Clark type oxygen elect...) | 32 Resp O2 std dev [±] (Calculated) | 33 CSC flag (Calculated using seacarb afte...) | 34 pH (Total scale, Calculated using...) | 35 CO2 [µmol/kg] (Calculated using seacarb afte...) | 36 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 37 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 38 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 39 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 40 Omega Arg (Calculated using seacarb afte...) | 41 Omega Cal (Calculated using seacarb afte...) |
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L-C | Before renewal | 35 | 20 | 39.3 | 1.1 | 8.18 | 0.04 | 1976.2 | 7.6 | 1768.0 | 19.8 | 195.7 | 31.9 | 12.5 | 1.4 | 2255.9 | 54.9 | 0.293 | 0.005 | 103.8 | 1.2 | 353.9 | 6.9 | 0.315 | 0.028 | 1.223 | 0.062 | 0.347 | 0.016 | 85.57 | 23.10 | 27 | 8.19 | 9.52 | 283.68 | 282.73 | 1761.17 | 205.52 | 3.29 | 5.16 |
H-C | Before renewal | 35 | 20 | 101.3 | 3.0 | 7.82 | 0.02 | 2137.8 | 13.2 | 2008.3 | 12.4 | 97.0 | 8.2 | 32.4 | 1.9 | 2251.5 | 41.5 | 0.295 | 0.006 | 103.7 | 1.4 | 351.5 | 8.1 | 0.314 | 0.026 | 1.286 | 0.049 | 0.385 | 0.026 | 115.01 | 24.02 | 27 | 7.81 | 25.81 | 769.26 | 766.68 | 2012.98 | 99.01 | 1.59 | 2.49 |
L-C-H-C | After renewal | 35 | 20 | 39.3 | 1.1 | 8.16 | 0.03 | 1998.1 | 11.5 | 1795.2 | 4.7 | 189.6 | 21.8 | 13.3 | 1.2 | 2273.5 | 47.7 | 0.288 | 0.007 | 108.3 | 1.4 | 376.0 | 8.0 | 0.409 | 0.013 | 27 | 8.18 | 9.89 | 294.74 | 293.76 | 1785.02 | 203.19 | 3.25 | 5.10 | ||||||
H-C-L-C | After renewal | 35 | 20 | 101.3 | 3.0 | 7.80 | 0.02 | 2154.7 | 15.9 | 2026.9 | 15.6 | 93.5 | 8.3 | 34.3 | 1.9 | 2266.9 | 44.5 | 0.289 | 0.008 | 99.9 | 1.4 | 345.7 | 8.4 | 0.365 | 0.005 | 27 | 7.81 | 26.36 | 785.90 | 783.27 | 2029.80 | 98.54 | 1.58 | 2.47 |