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Wu, YaPing; Gao, Kunshan; Riebesell, Ulf (2010): Seawater carbonate chemistry and diatom Phaeodactylum tricornutum (CCMA 106) biological processes during experiments, 2010. PANGAEA, https://doi.org/10.1594/PANGAEA.758214, Supplement to: Wu, YP et al. (2010): CO2-induced seawater acidification affects physiological performance of the marine diatom Phaeodactylum tricornutum. Biogeosciences, 7(9), 2915-2923, https://doi.org/10.5194/bg-7-2915-2010

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Abstract:
CO2/pH perturbation experiments were carried out under two different pCO2 levels (39.3 and 101.3 Pa) to evaluate effects of CO2-induced ocean acidification on the marine diatom Phaeodactylum tricornutum. After acclimation (>20 generations) to ambient and elevated CO2 conditions (with corresponding pH values of 8.15 and 7.80, respectively), growth and photosynthetic carbon fixation rates of high CO2 grown cells were enhanced by 5% and 12%, respectively, and dark respiration stimulated by 34% compared to cells grown at ambient CO2. The half saturation constant (Km) for carbon fixation (dissolved inorganic carbon, DIC) increased by 20% under the low pH and high CO2 condition, reflecting a decreased affinity for HCO3- or/and CO2 and down-regulated carbon concentrating mechanism (CCM). In the high CO2 grown cells, the electron transport rate from photosystem II (PSII) was photoinhibited to a greater extent at high levels of photosynthetically active radiation, while non-photochemical quenching was reduced compared to low CO2 grown cells. This was probably due to the down-regulation of CCM, which serves as a sink for excessive energy. The balance between these positive and negative effects on diatom productivity will be a key factor in determining the net effect of rising atmospheric CO2 on ocean primary production.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species
Funding:
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Experimental treatmentExp treatWu, YaPing
CommentCommentWu, YaPing
SalinitySalWu, YaPingMeasured
Temperature, waterTemp°CWu, YaPingMeasured
Carbon dioxide, partial pressurepCO2PaWu, YaPingInfrared CO2/H2O gas analyzer, non-dispersive, LI-COR type LI-7000
Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Wu, YaPing
pHpHWu, YaPingpH meter (Benchtop pH510, OAKTON)NBS scale
pH, standard deviationpH std dev±Wu, YaPing
Carbon, inorganic, dissolvedDICµmol/kgWu, YaPingDIC analyzer (AS-C3, Apollo Scitech)
10 Carbon, inorganic, dissolved, standard deviationDIC std dev±Wu, YaPing
11 Bicarbonate ion[HCO3]-µmol/kgWu, YaPingCalculated using CO2SYS
12 Bicarbonate ion, standard deviation[HCO3]- std dev±Wu, YaPing
13 Carbonate ion[CO3]2-µmol/kgWu, YaPingCalculated using CO2SYS
14 Carbonate ion, standard deviation[CO3]2- std dev±Wu, YaPing
15 Carbon dioxideCO2µmol/kgWu, YaPingCalculated using CO2SYS
16 Carbon dioxide, standard deviationCO2 std dev±Wu, YaPing
17 Alkalinity, totalATµmol/kgWu, YaPingCalculated using CO2SYS
18 Alkalinity, total, standard deviationAT std dev±Wu, YaPing
19 Photochemical efficiencydelta F/Fmarbitrary unitsWu, YaPingCalculated, see reference(s)
20 Photochemical efficiency, standard deviationdelta F/Fm std dev±Wu, YaPing
21 Maximum potential photosynthesisPmaxWu, YaPingsee reference(s)
22 Maximum potential photosynthesis, standard deviationPmax std dev±Wu, YaPing
23 Light saturation pointIkWu, YaPingsee reference(s)
24 Light saturation point, standard deviationIk std dev±Wu, YaPing
25 Chlorophyll a per cellChl a/cellpg/#Wu, YaPingSpectrophotometry
26 Chlorophyll a, standard deviationChl a std dev±Wu, YaPing
27 Growth rateµ#/dayWu, YaPingCalculated
28 Growth rate, standard deviationµ std dev±Wu, YaPing
29 Photosynthetic carbon fixation rateC fixµmol/µg/hWu, YaPingLiquid scintillation counter (Tri-Carb 2800TR, Perkin-Elmer)
30 Photosynthetic carbon fixation rate, standard deviationC fix std dev±Wu, YaPing
31 Respiration rate, oxygenResp O2µmol/mg/hWu, YaPingClark type oxygen electrode (5300A, YSI)Dark
32 Respiration rate, oxygen, standard deviationResp O2 std dev±Wu, YaPingCalculated
33 Carbonate system computation flagCSC flagNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
34 pHpHNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)Total scale
35 Carbon dioxideCO2µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
36 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
37 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
38 Bicarbonate ion[HCO3]-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
39 Carbonate ion[CO3]2-µmol/kgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
40 Aragonite saturation stateOmega ArgNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
41 Calcite saturation stateOmega CalNisumaa, Anne-MarinCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
152 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Exp treat

Comment

Sal
(Measured)

Temp [°C]
(Measured)

pCO2 [Pa]
(Infrared CO2/H2O gas analyzer...)

pCO2 std dev [±]

pH
(NBS scale, pH meter (Benchtop...)

pH std dev [±]

DIC [µmol/kg]
(DIC analyzer (AS-C3, Apollo S...)
10 
DIC std dev [±]
11 
[HCO3]- [µmol/kg]
(Calculated using CO2SYS)
12 
[HCO3]- std dev [±]
13 
[CO3]2- [µmol/kg]
(Calculated using CO2SYS)
14 
[CO3]2- std dev [±]
15 
CO2 [µmol/kg]
(Calculated using CO2SYS)
16 
CO2 std dev [±]
17 
AT [µmol/kg]
(Calculated using CO2SYS)
18 
AT std dev [±]
19 
delta F/Fm [arbitrary units]
(Calculated, see reference(s))
20 
delta F/Fm std dev [±]
21 
Pmax
(see reference(s))
22 
Pmax std dev [±]
23 
Ik
(see reference(s))
24 
Ik std dev [±]
25 
Chl a/cell [pg/#]
(Spectrophotometry)
26 
Chl a std dev [±]
27 
µ [#/day]
(Calculated)
28 
µ std dev [±]
29 
C fix [µmol/µg/h]
(Liquid scintillation counter ...)
30 
C fix std dev [±]
31 
Resp O2 [µmol/mg/h]
(Dark, Clark type oxygen elect...)
32 
Resp O2 std dev [±]
(Calculated)
33 
CSC flag
(Calculated using seacarb afte...)
34 
pH
(Total scale, Calculated using...)
35 
CO2 [µmol/kg]
(Calculated using seacarb afte...)
36 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
37 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
38 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)
39 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
40 
Omega Arg
(Calculated using seacarb afte...)
41 
Omega Cal
(Calculated using seacarb afte...)
L-CBefore renewal352039.31.18.180.041976.27.61768.019.8195.731.912.51.42255.954.90.2930.005103.81.2353.96.90.3150.0281.2230.0620.3470.01685.5723.10278.199.52283.68282.731761.17205.523.295.16
H-CBefore renewal3520101.33.07.820.022137.813.22008.312.497.08.232.41.92251.541.50.2950.006103.71.4351.58.10.3140.0261.2860.0490.3850.026115.0124.02277.8125.81769.26766.682012.9899.011.592.49
L-C-H-CAfter renewal352039.31.18.160.031998.111.51795.24.7189.621.813.31.22273.547.70.2880.007108.31.4376.08.00.4090.013278.189.89294.74293.761785.02203.193.255.10
H-C-L-CAfter renewal3520101.33.07.800.022154.715.92026.915.693.58.334.31.92266.944.50.2890.00899.91.4345.78.40.3650.005277.8126.36785.90783.272029.8098.541.582.47