/* DATA DESCRIPTION: Citation: Wu, YaPing; Gao, Kunshan; Riebesell, Ulf (2010): Seawater carbonate chemistry and diatom Phaeodactylum tricornutum (CCMA 106) biological processes during experiments, 2010. PANGAEA, https://doi.org/10.1594/PANGAEA.758214, Supplement to: Wu, YP et al. (2010): CO2-induced seawater acidification affects physiological performance of the marine diatom Phaeodactylum tricornutum. Biogeosciences, 7(9), 2915-2923, https://doi.org/10.5194/bg-7-2915-2010 Abstract: CO2/pH perturbation experiments were carried out under two different pCO2 levels (39.3 and 101.3 Pa) to evaluate effects of CO2-induced ocean acidification on the marine diatom Phaeodactylum tricornutum. After acclimation (>20 generations) to ambient and elevated CO2 conditions (with corresponding pH values of 8.15 and 7.80, respectively), growth and photosynthetic carbon fixation rates of high CO2 grown cells were enhanced by 5% and 12%, respectively, and dark respiration stimulated by 34% compared to cells grown at ambient CO2. The half saturation constant (Km) for carbon fixation (dissolved inorganic carbon, DIC) increased by 20% under the low pH and high CO2 condition, reflecting a decreased affinity for HCO3- or/and CO2 and down-regulated carbon concentrating mechanism (CCM). In the high CO2 grown cells, the electron transport rate from photosystem II (PSII) was photoinhibited to a greater extent at high levels of photosynthetically active radiation, while non-photochemical quenching was reduced compared to low CO2 grown cells. This was probably due to the down-regulation of CCM, which serves as a sink for excessive energy. The balance between these positive and negative effects on diatom productivity will be a key factor in determining the net effect of rising atmospheric CO2 on ocean primary production. Keyword(s): Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species Project(s): European network of excellence for Ocean Ecosystems Analysis (EUR-OCEANS) (URI: https://eur-oceans.eu/) European Project on Ocean Acidification (EPOCA) (URI: https://en.wikipedia.org/wiki/European_Project_on_Ocean_Acidification) Ocean Acidification International Coordination Centre (OA-ICC) (URI: https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management) Funding: Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification (URI: https://cordis.europa.eu/project/id/211384) Sixth Framework Programme (FP6), grant/award no. 511106: European network of excellence for Ocean Ecosystems Analysis (URI: https://cordis.europa.eu/project/id/511106) Comment: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). Parameter(s): Experimental treatment (Exp treat) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Comment (Comment) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Salinity (Sal) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Measured Temperature, water [°C] (Temp) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Measured Carbon dioxide, partial pressure [Pa] (pCO2) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Infrared CO2/H2O gas analyzer, non-dispersive, LI-COR type LI-7000 Carbon dioxide, partial pressure, standard deviation [±] (pCO2 std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) pH (pH) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: pH meter (Benchtop pH510, OAKTON) * COMMENT: NBS scale pH, standard deviation [±] (pH std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: DIC analyzer (AS-C3, Apollo Scitech) Carbon, inorganic, dissolved, standard deviation [±] (DIC std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Bicarbonate ion, standard deviation [±] ([HCO3]- std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Carbonate ion [µmol/kg] ([CO3]2-) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Carbonate ion, standard deviation [±] ([CO3]2- std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Carbon dioxide [µmol/kg] (CO2) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Carbon dioxide, standard deviation [±] (CO2 std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Alkalinity, total [µmol/kg] (AT) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) Alkalinity, total, standard deviation [±] (AT std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Photochemical efficiency [arbitrary units] (delta F/Fm) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Calculated, see reference(s) Photochemical efficiency, standard deviation [±] (delta F/Fm std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Maximum potential photosynthesis (Pmax) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: see reference(s) Maximum potential photosynthesis, standard deviation [±] (Pmax std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Light saturation point (Ik) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: see reference(s) Light saturation point, standard deviation [±] (Ik std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Chlorophyll a per cell [pg/#] (Chl a/cell) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Spectrophotometry Chlorophyll a, standard deviation [±] (Chl a std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Growth rate [#/day] (µ) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Calculated Growth rate, standard deviation [±] (µ std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Photosynthetic carbon fixation rate [µmol/µg/h] (C fix) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Liquid scintillation counter (Tri-Carb 2800TR, Perkin-Elmer) Photosynthetic carbon fixation rate, standard deviation [±] (C fix std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) Respiration rate, oxygen [µmol/mg/h] (Resp O2) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Clark type oxygen electrode (5300A, YSI) * COMMENT: Dark Respiration rate, oxygen, standard deviation [±] (Resp O2 std dev) * PI: Wu, YaPing (ypwu.xmu@gmail.com) * METHOD/DEVICE: Calculated Carbonate system computation flag (CSC flag) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) pH (pH) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: Total scale Carbon dioxide [µmol/kg] (CO2) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Fugacity of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (fCO2water_SST_wet) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Carbonate ion [µmol/kg] ([CO3]2-) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Aragonite saturation state (Omega Arg) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) Calcite saturation state (Omega Cal) * PI: Nisumaa, Anne-Marin (annemarin@gmail.com) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Status: Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) Size: 152 data points */ Exp treat Comment Sal (Measured) Temp [°C] (Measured) pCO2 [Pa] (Infrared CO2/H2O gas analyzer...) pCO2 std dev [±] pH (NBS scale, pH meter (Benchtop...) pH std dev [±] DIC [µmol/kg] (DIC analyzer (AS-C3, Apollo S...) DIC std dev [±] [HCO3]- [µmol/kg] (Calculated using CO2SYS) [HCO3]- std dev [±] [CO3]2- [µmol/kg] (Calculated using CO2SYS) [CO3]2- std dev [±] CO2 [µmol/kg] (Calculated using CO2SYS) CO2 std dev [±] AT [µmol/kg] (Calculated using CO2SYS) AT std dev [±] delta F/Fm [arbitrary units] (Calculated, see reference(s)) delta F/Fm std dev [±] Pmax (see reference(s)) Pmax std dev [±] Ik (see reference(s)) Ik std dev [±] Chl a/cell [pg/#] (Spectrophotometry) Chl a std dev [±] µ [#/day] (Calculated) µ std dev [±] C fix [µmol/µg/h] (Liquid scintillation counter ...) C fix std dev [±] Resp O2 [µmol/mg/h] (Dark, Clark type oxygen elect...) Resp O2 std dev [±] (Calculated) CSC flag (Calculated using seacarb afte...) pH (Total scale, Calculated using...) CO2 [µmol/kg] (Calculated using seacarb afte...) pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) [HCO3]- [µmol/kg] (Calculated using seacarb afte...) [CO3]2- [µmol/kg] (Calculated using seacarb afte...) Omega Arg (Calculated using seacarb afte...) Omega Cal (Calculated using seacarb afte...) L-C Before renewal 35 20 39.3 1.1 8.18 0.04 1976.2 7.6 1768.0 19.8 195.7 31.9 12.5 1.4 2255.9 54.9 0.293 0.005 103.8 1.2 353.9 6.9 0.315 0.028 1.223 0.062 0.347 0.016 85.57 23.10 27 8.19 9.52 283.68 282.73 1761.17 205.52 3.29 5.16 H-C Before renewal 35 20 101.3 3.0 7.82 0.02 2137.8 13.2 2008.3 12.4 97.0 8.2 32.4 1.9 2251.5 41.5 0.295 0.006 103.7 1.4 351.5 8.1 0.314 0.026 1.286 0.049 0.385 0.026 115.01 24.02 27 7.81 25.81 769.26 766.68 2012.98 99.01 1.59 2.49 L-C-H-C After renewal 35 20 39.3 1.1 8.16 0.03 1998.1 11.5 1795.2 4.7 189.6 21.8 13.3 1.2 2273.5 47.7 0.288 0.007 108.3 1.4 376.0 8.0 0.409 0.013 27 8.18 9.89 294.74 293.76 1785.02 203.19 3.25 5.10 H-C-L-C After renewal 35 20 101.3 3.0 7.80 0.02 2154.7 15.9 2026.9 15.6 93.5 8.3 34.3 1.9 2266.9 44.5 0.289 0.008 99.9 1.4 345.7 8.4 0.365 0.005 27 7.81 26.36 785.90 783.27 2029.80 98.54 1.58 2.47