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Stock, Lennart; Wegener, Gunter; Akeerath Mundanatt, Aman; Altun, Jenny; Dirksen, Lukas; Elvert, Marcus (2026): Amino acids from cold seep core MUC09-3 and reference core MUC08-4 at Astoria Canyon, Cascadia Margin, Oregon USA [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.997006 (DOI registration in progress)

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Published: 2026-09-02

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Abstract:
This dataset contains measurements of lipid biomarkers, disaccharides, monosaccharides, amino acids, and total organic carbon (TOC) in sediments and pore fluids collected from a cold seep at Astoria Canyon, Cascadia Margin, Oregon, USA. The data are part of a larger seep dataset comprising complementary geochemical and molecular measurements. Associated data are available through the Biological and Chemical Oceanography Data Management Office (BCO-DMO), including the dataset available under DOI 10.26008/1912/bco-dmo.959765.1. 16S rRNA gene amplicon sequencing data are available through the European Nucleotide Archive (ENA; PRJEB97184). The data presented here are archived in PANGAEA and comprise measurements of lipid biomarkers, carbohydrates, amino acids, and total organic carbon in the analyzed sediment and pore-fluid samples.
Keyword(s):
amino acids; ANME-2; AOM; Cold seeps; Disaccharides; lipids; Monosaccharides; SRB; Sucrose; TOC; Trehalose
Related to:
Stock, Lennart; Wegener, Gunter; Pohlman, John W; Lapham, Laura L; Lloyd, Karen; Williams, L; Torset, Stian; Lalk, Ellen; Hildebrand, Anna; Dirksen, Lukas; Akeerath Mundanatt, Aman; Wilson, Rachel; Seewald, Jeffrey S; Liebeke, Manuel; Elvert, Marcus (preprint): WITHDRAWN: Disaccharide production in marine cold seeps links dissolved organic carbon to anaerobic oxidation of methane. https://doi.org/10.21203/rs.3.rs-9427392/v2
References:
H2 concentrations in highly active deep-sea methane seep sediments in Hydrate Ridge and Astoria Canyon (2026). European Nucleotide Archive (ENA), insdc:PRJEB97184
Lalk, Ellen; Pohlman, John W; Lapham, Laura L; Casso, Michael; Glenna, Madeline; Hildebrand, Anna; Lloyd, Karen; Ma, Kun; Seewald, Jeffrey S; Stock, Lennart; Strauss, Maureen A; Sylva, Sean P; Traver, Lily; Wilson, Rachel; Williams, L (2025): Porewater dissolved organic carbon and associated geochemical data for methane seeps in the Cascadia Margin: Astoria Canyon, Barkley Canyon, Hydrate Ridge, and Bullseye Vent [dataset]. Biological and Chemical Oceanography Data Management Office (BCO-DMO), https://doi.org/10.26008/1912/BCO-DMO.959765.1
Lapham, Laura L (2023): AT50-14, Investigating the source and flux of dissolved organic carbon released from methane seeps to the deep-ocean [dataset]. Rolling Deck to Repository (R2R) Program, https://doi.org/10.7284/910142
Funding:
German Research Foundation (DFG), grant/award no. 390741603: EXC 2077: The Ocean Floor – Earth's Uncharted Interface
National Energy Technology Laboratory, grant/award no. 89243320SFE000013: SeepDOM
National Science Foundation (NSF), grant/award no. OCE-2049517, OCE-2048357, and OCE-2048831: SeepDOM
Coverage:
Median Latitude: 46.240765 * Median Longitude: -124.614000 * South-bound Latitude: 46.240740 * West-bound Longitude: -124.625000 * North-bound Latitude: 46.240790 * East-bound Longitude: -124.603000
Date/Time Start: 2023-08-17T00:00:00 * Date/Time End: 2023-08-19T00:00:00
Minimum DEPTH, sediment/rock: 0.015 m * Maximum DEPTH, sediment/rock: 0.465 m
Event(s):
AT50-14_MUC08-4 * Latitude: 46.240740 * Longitude: -124.625000 * Date/Time: 2023-08-17T00:00:00 * Elevation: -822.6 m * Location: Astoria Canyon, Oregon, USA * Campaign: AT50-14 (SeepDOM) * Basis: Atlantis (1997) * Method/Device: MultiCorer (MUC)
AT50-14_MUC09-3 (H867) * Latitude: 46.240790 * Longitude: -124.603000 * Date/Time: 2023-08-19T00:00:00 * Elevation: -759.6 m * Location: Astoria Canyon, Oregon, USA * Campaign: AT50-14 (SeepDOM) * Basis: Atlantis (1997) * Method/Device: MultiCorer (MUC)
Comment:
Concentrations are expressed in µg/g sediment dry weight.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventStock, Lennart
2IdentificationIDStock, Lennart
3TypeTypeStock, Lennart
4DEPTH, sediment/rockDepth sedmStock, LennartGeocode – mbsf
5Alanine, per unit sediment massAla/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
6Glycine, per unit sediment massGly/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
7Valine, per unit sediment massVal/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
8Leucine, per unit sediment massLeu/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
9Isoleucine, per unit sediment massIle/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
10Proline, per unit sediment massPro/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
11Aspartic acid, per unit sediment massAsp/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
12Threonine, per unit sediment massThr/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
13Serine, per unit sediment massSer/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
14Methionine, per unit sediment massMet/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
15Asparagine per unit sediment massAsparagine/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
16Phenylalanine, per unit sediment massPhe/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
17Tyrosine, per unit sediment massTyr/sedµg/gStock, LennartGas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
18Alanine, δ13CAla δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
19Glycine, δ13CGly δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
20Valine, δ13CVal δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
21Leucine, δ13CLeu δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
22Isoleucine, δ13CIle δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
23Proline, δ13CPro δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
24Aspartic acid, δ13CAsp δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
25Threonine, δ13CThr δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
26Serine, δ13CSer δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
27Methionine, δ13CMet δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
28Asparagine, δ13CAsn δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
29Phenylalanine, δ13CPhe δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
30Tyrosine, δ13CTyr δ13C‰ PDBStock, LennartGas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
448 data points

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