/* DATA DESCRIPTION:
Citation:	Stock, Lennart; Wegener, Gunter; Akeerath Mundanatt, Aman; Altun, Jenny; Dirksen, Lukas; Elvert, Marcus (2026): Amino acids from cold seep core MUC09-3 and reference core MUC08-4 at Astoria Canyon, Cascadia Margin, Oregon USA [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.997006 (DOI registration in progress)
Abstract:	This dataset contains measurements of lipid biomarkers, disaccharides, monosaccharides, amino acids, and total organic carbon (TOC) in sediments and pore fluids collected from a cold seep at Astoria Canyon, Cascadia Margin, Oregon, USA. The data are part of a larger seep dataset comprising complementary geochemical and molecular measurements. Associated data are available through the Biological and Chemical Oceanography Data Management Office (BCO-DMO), including the dataset available under DOI 10.26008/1912/bco-dmo.959765.1. 16S rRNA gene amplicon sequencing data are available through the European Nucleotide Archive (ENA; PRJEB97184). The data presented here are archived in PANGAEA and comprise measurements of lipid biomarkers, carbohydrates, amino acids, and total organic carbon in the analyzed sediment and pore-fluid samples.
Keyword(s):	amino acids; ANME-2; AOM; Cold seeps; Disaccharides; lipids; Monosaccharides; SRB; Sucrose; TOC; Trehalose
Related to:	Stock, Lennart; Wegener, Gunter; Pohlman, John W; Lapham, Laura L; Lloyd, Karen; Williams, L; Torset, Stian; Lalk, Ellen; Hildebrand, Anna; Dirksen, Lukas; Akeerath Mundanatt, Aman; Wilson, Rachel; Seewald, Jeffrey S; Liebeke, Manuel; Elvert, Marcus (preprint): WITHDRAWN: Disaccharide production in marine cold seeps links dissolved organic carbon to anaerobic oxidation of methane. https://doi.org/10.21203/rs.3.rs-9427392/v2
References:	H2 concentrations in highly active deep-sea methane seep sediments in Hydrate Ridge and Astoria Canyon (2026). European Nucleotide Archive (ENA), insdc:PRJEB97184
	Lalk, Ellen; Pohlman, John W; Lapham, Laura L; Casso, Michael; Glenna, Madeline; Hildebrand, Anna; Lloyd, Karen; Ma, Kun; Seewald, Jeffrey S; Stock, Lennart; Strauss, Maureen A; Sylva, Sean P; Traver, Lily; Wilson, Rachel; Williams, L (2025): Porewater dissolved organic carbon and associated geochemical data for methane seeps in the Cascadia Margin: Astoria Canyon, Barkley Canyon, Hydrate Ridge, and Bullseye Vent [dataset]. Biological and Chemical Oceanography Data Management Office (BCO-DMO), https://doi.org/10.26008/1912/BCO-DMO.959765.1
	Lapham, Laura L (2023): AT50-14, Investigating the source and flux of dissolved organic carbon released from methane seeps to the deep-ocean [dataset]. Rolling Deck to Repository (R2R) Program, https://doi.org/10.7284/910142
Funding:	German Research Foundation (DFG) (URI: http://www.dfg.de/en/), grant/award no. 390741603: EXC 2077: The Ocean Floor – Earth's Uncharted Interface (URI: https://gepris.dfg.de/project/390741603)
	National Energy Technology Laboratory, grant/award no. 89243320SFE000013: SeepDOM
	National Science Foundation (NSF), grant/award no. OCE-2049517, OCE-2048357, and OCE-2048831: SeepDOM
Coverage:	MEDIAN LATITUDE: 46.240765 * MEDIAN LONGITUDE: -124.614000 * SOUTH-BOUND LATITUDE: 46.240740 * WEST-BOUND LONGITUDE: -124.625000 * NORTH-BOUND LATITUDE: 46.240790 * EAST-BOUND LONGITUDE: -124.603000
	DATE/TIME START: 2023-08-17T00:00:00 * DATE/TIME END: 2023-08-19T00:00:00
	MINIMUM DEPTH, sediment/rock: 0.015 m * MAXIMUM DEPTH, sediment/rock: 0.465 m
Event(s):	AT50-14_MUC08-4 * LATITUDE: 46.240740 * LONGITUDE: -124.625000 * DATE/TIME: 2023-08-17T00:00:00 * ELEVATION: -822.6 m * LOCATION: Astoria Canyon, Oregon, USA * CAMPAIGN: AT50-14 (SeepDOM) (URI: https://doi.org/10.7284/910142) * BASIS: Atlantis (1997) (URI: https://www.whoi.edu/what-we-do/explore/ships/ships-atlantis/) * METHOD/DEVICE: MultiCorer (MUC)
	AT50-14_MUC09-3 (H867) * LATITUDE: 46.240790 * LONGITUDE: -124.603000 * DATE/TIME: 2023-08-19T00:00:00 * ELEVATION: -759.6 m * LOCATION: Astoria Canyon, Oregon, USA * CAMPAIGN: AT50-14 (SeepDOM) (URI: https://doi.org/10.7284/910142) * BASIS: Atlantis (1997) (URI: https://www.whoi.edu/what-we-do/explore/ships/ships-atlantis/) * METHOD/DEVICE: MultiCorer (MUC)
Comment:	Concentrations are expressed in µg/g sediment dry weight.
Parameter(s):	Event label (Event) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127)
	Identification (ID) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127)
	Type (Type) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127)
	DEPTH, sediment/rock [m] (Depth sed) * GEOCODE * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * COMMENT: mbsf
	Alanine, per unit sediment mass [µg/g] (Ala/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Glycine, per unit sediment mass [µg/g] (Gly/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Valine, per unit sediment mass [µg/g] (Val/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Leucine, per unit sediment mass [µg/g] (Leu/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Isoleucine, per unit sediment mass [µg/g] (Ile/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Proline, per unit sediment mass [µg/g] (Pro/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Aspartic acid, per unit sediment mass [µg/g] (Asp/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Threonine, per unit sediment mass [µg/g] (Thr/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Serine, per unit sediment mass [µg/g] (Ser/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Methionine, per unit sediment mass [µg/g] (Met/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Asparagine per unit sediment mass [µg/g] (Asparagine/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Phenylalanine, per unit sediment mass [µg/g] (Phe/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Tyrosine, per unit sediment mass [µg/g] (Tyr/sed) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatography - Flame Ionization Detection (GC-FID), Thermo Scientific, Trace GC Ultra [Rxi-5ms capillary column]; identification by GC-MS, Thermo Scientific Trace 1310; coupled with ISQ 700
	Alanine, δ13C [‰ PDB] (Ala δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Glycine, δ13C [‰ PDB] (Gly δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Valine, δ13C [‰ PDB] (Val δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Leucine, δ13C [‰ PDB] (Leu δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Isoleucine, δ13C [‰ PDB] (Ile δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Proline, δ13C [‰ PDB] (Pro δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Aspartic acid, δ13C [‰ PDB] (Asp δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Threonine, δ13C [‰ PDB] (Thr δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Serine, δ13C [‰ PDB] (Ser δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Methionine, δ13C [‰ PDB] (Met δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Asparagine, δ13C [‰ PDB] (Asn δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Phenylalanine, δ13C [‰ PDB] (Phe δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
	Tyrosine, δ13C [‰ PDB] (Tyr δ13C) * PI: Stock, Lennart (https://orcid.org/0009-0004-3039-5127) * METHOD/DEVICE: Gas chromatograph–isotope ratio mass spectrometer (GC-IRMS), Thermo Scientific, Trace GC Ultra; coupled with GC IsoLink and ConFlow IV to Delta V Plus IRMS [Restek Rxi-5ms column]
License:	Creative Commons Attribution 4.0 International (CC-BY-4.0) (URI: https://creativecommons.org/licenses/by/4.0/)
Status:	Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels)
Size:	448 data points
*/
Event	ID	Type	Depth sed [m]	Ala/sed [µg/g]	Gly/sed [µg/g]	Val/sed [µg/g]	Leu/sed [µg/g]	Ile/sed [µg/g]	Pro/sed [µg/g]	Asp/sed [µg/g]	Thr/sed [µg/g]	Ser/sed [µg/g]	Met/sed [µg/g]	Asparagine/sed [µg/g]	Phe/sed [µg/g]	Tyr/sed [µg/g]	Ala δ13C [‰ PDB]	Gly δ13C [‰ PDB]	Val δ13C [‰ PDB]	Leu δ13C [‰ PDB]	Ile δ13C [‰ PDB]	Pro δ13C [‰ PDB]	Asp δ13C [‰ PDB]	Thr δ13C [‰ PDB]	Ser δ13C [‰ PDB]	Met δ13C [‰ PDB]	Asn δ13C [‰ PDB]	Phe δ13C [‰ PDB]	Tyr δ13C [‰ PDB]
AT50-14_MUC08-4	276	Reference	0.060	23.5	61.0	12.8	26.6	7.3	24.4	66.9	26.2	42.9	4.7	22.9	16.1	11.0	-29	-20	-36	-34	-30	-19	-22	-29	-28	-31	-21	-30	-32
AT50-14_MUC08-4	278	Reference	0.160	31.1	65.7	8.2	24.3	3.9	15.6	61.1	5.6	7.9	3.2	4.3	13.5	5.7	-28	-22	-31	-35	-29	-20	-23	-28	-28	-31	-20	-31	-32
AT50-14_MUC08-4	280	Reference	0.260	35.6	67.9	7.6	22.0	3.3	20.1	60.1	5.5	8.9	3.1	6.9	12.3	4.5	-31	-22	-36	-35	-33	-18	-24	-29	-28	-31	-21	-30	-32
AT50-14_MUC08-4	282	Reference	0.360	29.6	65.7	6.9	21.2	3.0	21.2	52.5	2.8	3.4	3.1	9.8	12.2	2.6	-32	-25	-37	-36	-27	-17	-27	-32	-29	-31	-23	-33	-29
AT50-14_MUC08-4	284	Reference	0.465	16.6	32.6	5.6	18.5	3.0	7.4	60.9	16.3	32.0	4.4	12.5	11.5	6.7	-29	-24	-37	-33	-28	-16	-25	-31	-28	-29	-20	-30	-31
AT50-14_MUC09-3	335	Cold seep	0.015	56.0	105.4	13.4	43.8	7.0	39.0	85.0	7.6	12.7	3.8	35.2	24.8	8.5	-27	-20	-38	-36	-35	-19	-25	-29	-29	-32	-23	-30	-32
AT50-14_MUC09-3	336	Cold seep	0.045	52.4	98.3	13.2	43.8	7.0	38.1	80.8	6.6	11.7	3.7	34.4	23.6	8.5	-30	-26	-42	-41	-36	-19	-27	-32	-30	-33	-27	-33	-34
AT50-14_MUC09-3	337	Cold seep	0.075	50.0	82.7	15.0	46.4	9.0	11.4	82.1	24.6	49.4	4.2	31.5	25.5	17.6	-39	-26	-54	-58	-54	-24	-33	-37	-33	-32	-37	-42	-40
AT50-14_MUC09-3	338	Cold seep	0.105	59.8	85.7	15.5	43.8	8.7	31.4	80.1	16.2	28.1	4.3	25.6	25.7	13.4	-45	-29	-58	-63	-61	-32	-36	-41	-38	-33	-42	-46	-46
AT50-14_MUC09-3	339	Cold seep	0.135	54.0	94.0	13.1	42.7	6.9	33.0	84.6	7.4	12.5	3.9	36.5	25.4	9.8	-39	-27	-54	-58	-57	-31	-33	-36	-34	-34	-40	-43	-41
AT50-14_MUC09-3	340	Cold seep	0.165	37.1	67.3	17.3	37.5	10.2	36.8	80.2	24.0	31.3	4.7	23.8	18.9	13.8	-39	-29	-55	-55	-55	-27	-32	-35	-33	-34	-39	-40	-39
AT50-14_MUC09-3	341	Cold seep	0.195	53.2	83.0	13.4	41.8	7.0	28.8	84.4	7.9	12.1	3.6	48.5	22.0	9.0	-44	-28	-56	-62	-63	-30	-36	-41	-38	-31	-43	-46	-45
AT50-14_MUC09-3	342	Cold seep	0.225	49.6	81.0	11.6	33.3	5.4	27.0	74.7	5.3	9.1	3.8	14.4	17.3	6.1	-42	-27	-54	-58	-54	-29	-31	-35	-32	-31	-36	-41	-41
AT50-14_MUC09-3	343	Cold seep	0.255	26.5	51.5	7.2	25.0	3.9	9.9	52.9	4.2	5.2	4.1	13.9	9.8	2.6	-33	-25	-43	-43	-39	-19	-27	-31	-25	-33	-26	-38	-36
AT50-14_MUC09-3	344	Cold seep	0.285	27.7	52.4	8.8	24.1	4.3	14.3	53.4	4.2	6.5	3.5	18.1	10.8	4.0	-27	-24	-38	-42	-37	-18	-26	-34	-28	-28	-26	-32	-34
AT50-14_MUC09-3	345	Cold seep	0.315	26.3	50.6	7.6	21.6	3.8	12.8	49.1	3.8	5.8	3.8	16.3	10.8	2.6	-32	-21	-41	-40	-36	-21	-25	-28	-31	-29	-25	-34	-33