Smykala, Mike; Engelen, Bert; Matthiessen, Birte; Lõhmus, Kertu; Pieck, Daniela; Moorthi, Stefanie D: Chlorophyll a, extracellular polymeric substance concentration and 16S rRNA gene copy numbers in saltmarsh sediments in response to a storm surge [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.992901 (dataset in review)
Abstract:
Samples were taken to study the effect of storm surges on ecosystem functioning of salt marsh microbial communities. Sediment samples were collected from experimental salt marsh islands located in the back-barrier tidal flats of Spiekeroog Island, German North Sea (53°45′N, 7°43′E). The islands consist of three elevation zones (0.7 m, 1.0 m, and 1.3 m above mean sea level), corresponding to pioneer zone, lower salt marsh, and upper salt marsh. Six islands were sampled (three initially bare; three transplanted with lower salt marsh sediment and vegetation). Sampling was conducted in September 2022 (pre-disturbance), March 2023 (post-winter storm surges), and August 2023 (recovery phase).
Surface sediments (upper 2 cm) were collected using syringe cores. Pooled samples were analyzed for chlorophyll a as a proxy for microphytobenthos biomass using ethanol extraction and spectrophotometric pigment analysis. Extracellular polymeric substances (EPS) were quantified using EDTA extraction followed by phenol–sulfuric acid carbohydrate analysis. DNA was extracted from sediment subsamples using a Qiagen PowerSoil kit. Prokaryotic abundance was estimated by quantitative PCR targeting the 16S rRNA gene (primers 519F/907R), using an Escherichia coli 16S rRNA gene standard curve. The dataset includes chlorophyll a concentrations (µg g⁻¹ dry sediment), EPS carbohydrate concentrations, and prokaryotic 16S rRNA gene copy numbers for all sampling times, elevations, and treatments.
Keyword(s):
Funding:
German Research Foundation (DFG), grant/award no. 379417748: FOR 2716: Spatial community ecology in highly dynamic landscapes: from island biogeography to metaecosystems
Coverage:
Median Latitude: 53.759216 * Median Longitude: 7.718324 * South-bound Latitude: 53.758410 * West-bound Longitude: 7.715559 * North-bound Latitude: 53.759840 * East-bound Longitude: 7.720940
Date/Time Start: 2022-09-22T11:00:00 * Date/Time End: 2023-08-22T11:00:00
Event(s):
StormSurgeRecovery * Latitude: 53.760667 * Longitude: 7.722268 * Date/Time: 2023-08-22T11:00:00 * Location: Spiekeroog, German Bight, North Sea * Method/Device: Syringe, plastic
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Date/Time of event | Date/Time | ||||
| 3 | Type of study | Study type | ||||
| 4 | Vegetation type | Vegetation type | Initial Vegetation experimental island was set up whit | |||
| 5 | Experiment number ID | Exp no ID | Experimental island identifier | |||
| 6 | Zone | Zone | Elevation zone classification | |||
| 7 | DEPTH, sediment/rock | Depth sed | m | Geocode | ||
| 8 | Depth, top/min | Depth top | m | |||
| 9 | Depth, bottom/max | Depth bot | m | |||
| 10 | Extrapolymeric substances | EPS | µg/g | Microplate reader, BioTek, Synergy MX | ||
| 11 | 16S rRNA gene, copy number, per unit sediment mass | 16S rRNA gene/sed m | #/g | Quantitative real-time PCR (qPCR) (QPCR) | ||
| 12 | Chlorophyll a, per unit sediment mass | Chl a/sed | µg/g | Microplate reader, BioTek, Synergy MX | ||
| 13 | ALTITUDE | Altitude | m | Geocode | ||
| 14 | LATITUDE | Latitude | Geocode | |||
| 15 | LONGITUDE | Longitude | Geocode | |||
| 16 | Sample elevation | Elevation | m |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
450 data points