Citation:

PANGAEA.992901 (dataset in review)

Name: Chlorophyll a, extracellular polymeric substance concentration and 16S rRNA gene copy numbers in saltmarsh sediments in response to a storm surge
License: https://creativecommons.org/licenses/by/4.0/
Keywords: bacterial abundance; DynaCom; experimental islands; Microphytobenthos; salt marsh; Spiekeroog; Storm surge

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Abstract:

Samples were taken to study the effect of storm surges on ecosystem functioning of salt marsh microbial communities. Sediment samples were collected from experimental salt marsh islands located in the back-barrier tidal flats of Spiekeroog Island, German North Sea (53°45′N, 7°43′E). The islands consist of three elevation zones (0.7 m, 1.0 m, and 1.3 m above mean sea level), corresponding to pioneer zone, lower salt marsh, and upper salt marsh. Six islands were sampled (three initially bare; three transplanted with lower salt marsh sediment and vegetation). Sampling was conducted in September 2022 (pre-disturbance), March 2023 (post-winter storm surges), and August 2023 (recovery phase).

Surface sediments (upper 2 cm) were collected using syringe cores. Pooled samples were analyzed for chlorophyll a as a proxy for microphytobenthos biomass using ethanol extraction and spectrophotometric pigment analysis. Extracellular polymeric substances (EPS) were quantified using EDTA extraction followed by phenol–sulfuric acid carbohydrate analysis. DNA was extracted from sediment subsamples using a Qiagen PowerSoil kit. Prokaryotic abundance was estimated by quantitative PCR targeting the 16S rRNA gene (primers 519F/907R), using an Escherichia coli 16S rRNA gene standard curve. The dataset includes chlorophyll a concentrations (µg g⁻¹ dry sediment), EPS carbohydrate concentrations, and prokaryotic 16S rRNA gene copy numbers for all sampling times, elevations, and treatments.

Keyword(s):

bacterial abundance; DynaCom; experimental islands; Microphytobenthos; salt marsh; Spiekeroog; Storm surge

Funding:

German Research Foundation (DFG), grant/award no. 379417748 : FOR 2716: Spatial community ecology in highly dynamic landscapes: from island biogeography to metaecosystems

Coverage:

Median Latitude: 53.759216 * Median Longitude: 7.718324 * South-bound Latitude: 53.758410 * West-bound Longitude: 7.715559 * North-bound Latitude: 53.759840 * East-bound Longitude: 7.720940

Date/Time Start: 2022-09-22T11:00:00 * Date/Time End: 2023-08-22T11:00:00

Event(s):

StormSurgeRecovery * Latitude: 53.760667 * Longitude: 7.722268 * Date/Time: 2023-08-22T11:00:00 * Location: Spiekeroog, German Bight, North Sea * Method/Device: Syringe, plastic

StormSurgeResistance * Latitude: 53.760667 * Longitude: 7.722268 * Date/Time: 2023-03-14T11:00:00 * Location: Spiekeroog, German Bight, North Sea * Method/Device: Syringe, plastic

StormSurgeStart * Latitude: 53.760667 * Longitude: 7.722268 * Date/Time: 2022-09-22T11:00:00 * Location: Spiekeroog, German Bight, North Sea * Method/Device: Syringe, plastic

Parameter(s):

#	Name	Short Name	Unit	Method/Device	Comment
1	Event label	Event
2	Date/Time of event	Date/Time
3	Type of study	Study type
4	Vegetation type	Vegetation type			Initial Vegetation experimental island was set up whit
5	Experiment number ID	Exp no ID			Experimental island identifier
6	Zone	Zone			Elevation zone classification
7	DEPTH, sediment/rock	Depth sed	m		Geocode
8	Depth, top/min	Depth top	m
9	Depth, bottom/max	Depth bot	m
10	Extrapolymeric substances	EPS	µg/g	Microplate reader, BioTek, Synergy MX
11	16S rRNA gene, copy number, per unit sediment mass	16S rRNA gene/sed m	#/g	Quantitative real-time PCR (qPCR) (QPCR)
12	Chlorophyll a, per unit sediment mass	Chl a/sed	µg/g	Microplate reader, BioTek, Synergy MX
13	ALTITUDE	Altitude	m		Geocode
14	LATITUDE	Latitude			Geocode
15	LONGITUDE	Longitude			Geocode
16	Sample elevation	Elevation	m

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

450 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Date/Time	3 Study type	4 Vegetation type	5 Exp no ID	6 Zone	7 Depth sed [m]	9 Depth bot [m]	10 EPS [µg/g]	11 16S rRNA gene/sed m [#/g]	12 Chl a/sed [µg/g]	13 Altitude [m]	14 Latitude	15 Longitude	16 Elevation [m]
StormSurgeStart	2022-09-22T11:00:00	Field observation	transplanted	7	pioneer	0.01	0.02	0.43	5160256410	4.19	1.4762	53.75843	7.720940	0.7
StormSurgeStart	2022-09-22T11:00:00	Field observation	bare	8	pioneer	0.01	0.02	0.46	16730769231	3.47	1.3797	53.75875	7.719080	0.7
StormSurgeStart	2022-09-22T11:00:00	Field observation	transplanted	9	pioneer	0.01	0.02	0.08	14900472335	1.15	1.5083	53.75922	7.719060	0.7
StormSurgeStart	2022-09-22T11:00:00	Field observation	bare	10	pioneer	0.01	0.02	2.08	2992645614	5.12	1.4120	53.75972	7.717690	0.7
StormSurgeStart	2022-09-22T11:00:00	Field observation	transplanted	11	pioneer	0.01	0.02	0.39	33421052632	2.83	1.4400	53.75958	7.716780	0.7
StormSurgeStart	2022-09-22T11:00:00	Field observation	bare	12	pioneer	0.01	0.02	0.66	9758906883	3.82	1.4144	53.75984	7.715650	0.7
StormSurgeStart	2022-09-22T11:00:00	Field observation	transplanted	7	LSM	0.01	0.02	0.59	2682456140	2.03	1.7641	53.75842	7.720920	1.0
StormSurgeStart	2022-09-22T11:00:00	Field observation	bare	8	LSM	0.01	0.02	1.10	23259789474	3.08	1.6877	53.75874	7.719120	1.0
StormSurgeStart	2022-09-22T11:00:00	Field observation	transplanted	9	LSM	0.01	0.02	0.42	15058479532	2.28	1.7413	53.75921	7.719020	1.0
StormSurgeStart	2022-09-22T11:00:00	Field observation	bare	10	LSM	0.01	0.02	0.02	174156433	1.69	1.7140	53.75982	7.717660	1.0
StormSurgeStart	2022-09-22T11:00:00	Field observation	transplanted	11	LSM	0.01	0.02	0.36	18045467836	2.83	1.7520	53.75956	7.716760	1.0
StormSurgeStart	2022-09-22T11:00:00	Field observation	bare	12	LSM	0.01	0.02	0.07	3881410526	1.88	1.7124	53.75982	7.715610	1.0
StormSurgeStart	2022-09-22T11:00:00	Field observation	transplanted	7	USM	0.01	0.02	0.03	122199333333	2.32	2.0071	53.75841	7.720890	1.3
StormSurgeStart	2022-09-22T11:00:00	Field observation	bare	8	USM	0.01	0.02	0.00	4674426451	0.42	1.8467	53.75873	7.719080	1.3
StormSurgeStart	2022-09-22T11:00:00	Field observation	transplanted	9	USM	0.01	0.02	0.00	11584795322	0.95	1.9673	53.75920	7.719000	1.3
StormSurgeStart	2022-09-22T11:00:00	Field observation	bare	10	USM	0.01	0.02	0.00	1949122807	0.68	2.0030	53.75969	7.717630	1.3
StormSurgeStart	2022-09-22T11:00:00	Field observation	transplanted	11	USM	0.01	0.02	0.59	84000000000	0.69	1.9859	53.75955	7.716730	1.3
StormSurgeStart	2022-09-22T11:00:00	Field observation	bare	12	USM	0.01	0.02	0.00	160819000000	0.26	1.9794	53.75980	7.715559	1.3
StormSurgeResistance	2023-03-14T11:00:00	Field observation	transplanted	7	pioneer	0.01	0.02	0.03	11140131579	0.99	1.4762	53.75843	7.720940	0.7
StormSurgeResistance	2023-03-14T11:00:00	Field observation	bare	8	pioneer	0.01	0.02	0.00	1389473684	0.56	1.3797	53.75875	7.719080	0.7
StormSurgeResistance	2023-03-14T11:00:00	Field observation	transplanted	9	pioneer	0.01	0.02	0.15	14076023392	0.78	1.5083	53.75922	7.719060	0.7
StormSurgeResistance	2023-03-14T11:00:00	Field observation	bare	10	pioneer	0.01	0.02	0.00	2317813765	0.32	1.4120	53.75972	7.717690	0.7
StormSurgeResistance	2023-03-14T11:00:00	Field observation	transplanted	11	pioneer	0.01	0.02	0.07	8560842	1.15	1.4400	53.75958	7.716780	0.7
StormSurgeResistance	2023-03-14T11:00:00	Field observation	bare	12	pioneer	0.01	0.02	0.00	2964181286	0.32	1.4144	53.75984	7.715650	0.7
StormSurgeResistance	2023-03-14T11:00:00	Field observation	transplanted	7	LSM	0.01	0.02	0.19	70614035088	1.33	1.7641	53.75842	7.720920	1.0
StormSurgeResistance	2023-03-14T11:00:00	Field observation	bare	8	LSM	0.01	0.02	1.14	365978333333	2.72	1.6877	53.75874	7.719120	1.0
StormSurgeResistance	2023-03-14T11:00:00	Field observation	transplanted	9	LSM	0.01	0.02	1.18	79665317139	1.94	1.7413	53.75921	7.719020	1.0
StormSurgeResistance	2023-03-14T11:00:00	Field observation	bare	10	LSM	0.01	0.02	1.90	49488304094	2.37	1.7140	53.75982	7.717660	1.0
StormSurgeResistance	2023-03-14T11:00:00	Field observation	transplanted	11	LSM	0.01	0.02	0.42	19280000000	1.88	1.7520	53.75956	7.716760	1.0
StormSurgeResistance	2023-03-14T11:00:00	Field observation	bare	12	LSM	0.01	0.02	0.75	15264170041	3.26	1.7124	53.75982	7.715610	1.0
StormSurgeResistance	2023-03-14T11:00:00	Field observation	transplanted	7	USM	0.01	0.02	2.49	79453441295	2.16	2.0071	53.75841	7.720890	1.3
StormSurgeResistance	2023-03-14T11:00:00	Field observation	transplanted	9	USM	0.01	0.02	0.23	2580157895	3.11	1.9673	53.75920	7.719000	1.3
StormSurgeResistance	2023-03-14T11:00:00	Field observation	transplanted	11	USM	0.01	0.02	2.89	289330526	2.38	1.9859	53.75955	7.716730	1.3
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	transplanted	7	pioneer	0.01	0.02	0.61	40495438596	3.73	1.4762	53.75843	7.720940	0.7
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	transplanted	9	pioneer	0.01	0.02	0.00	8905263158	3.14	1.5083	53.75922	7.719060	0.7
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	transplanted	11	pioneer	0.01	0.02	0.85	49632928475	3.45	1.4400	53.75958	7.716780	0.7
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	transplanted	7	LSM	0.01	0.02	2.71	42141970310	0.91	1.7641	53.75842	7.720920	1.0
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	bare	8	LSM	0.01	0.02	0.00	3161268556	0.42	1.6877	53.75874	7.719120	1.0
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	transplanted	9	LSM	0.01	0.02	0.98	17307692308	1.65	1.7413	53.75921	7.719020	1.0
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	bare	10	LSM	0.01	0.02	0.86	114213333333	1.51	1.7140	53.75982	7.717660	1.0
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	transplanted	11	LSM	0.01	0.02	0.25	4944534413	2.23	1.7520	53.75956	7.716760	1.0
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	bare	12	LSM	0.01	0.02	1.28	15890688259	1.35	1.7124	53.75982	7.715610	1.0
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	transplanted	7	USM	0.01	0.02	1.22	63134412955	4.38	2.0071	53.75841	7.720890	1.3
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	transplanted	9	USM	0.01	0.02	0.12	26568825911	2.51	1.9673	53.75920	7.719000	1.3
StormSurgeRecovery	2023-08-22T11:00:00	Field observation	transplanted	11	USM	0.01	0.02	1.87	66410931174	1.62	1.9859	53.75955	7.716730	1.3