Thielecke, Antonia; Narberhaus, Anna: Phytoplankton taxonomy from bioassays for carbon, nitrate and silica uptake of Arctic phytoplankton in response to light and silicic acid interactions (Microscopy) [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.991539 (dataset in review)
Abstract:
This data was collected during the Polarstern cruise PS138 (ArcWatch1) from August 3 to September 30, 2023 to the Central Arctic Ocean. At five stations during the Polarstern cruise PS138 (ArcWatch1) we conducted bioassays on board in order to assess the interactive effects of light and dSi on the uptake rates of carbon (C), nitrogen (N), and silicon (Si). For each bioassay we collected water samples from the chlorophyll maximum (Chl a max) using a CTD rosette, prefiltered the water with a 200 µm mesh and added different concentrations of dSi (0, 2, 5, 10, and 20 µmol L⁻¹ above ambient concentrations) to establish a dSi gradient. Samples were spiked with 13C labelled sodium bicarbonate, 15N labelled potassium nitrate and 30Si-sodium metasilicate at a 10% enrichment level. All samples were subsequently incubated for 24 hours under a light gradient (0, 10, 40, 80, 320 µmol photons m-2 s-1) creating a 5x5 gradient of light and dSi. After incubation, a subsample for microscopy was taken from the bottle incubated without added dSi at 10 µmol photons m-2 s-1 and preserved with hexamine-buffered formaldehyde (1-2% final concentration). 50 mL of sample were settled in an Utermöhl chamber (Utermöhl, 1958) and the different species were counted with an inverted light microscope (Zeiss IM 4717, Oberkochen, Germany). Identification to the lowest possible taxonomic level was based on Hoppenrath et al., (2009), Kraberg et al., (2010), Throndsen et al., (2007) and Tomas et al., (1997).
Keyword(s):
Related to:
Hoppenrath, Mona; Elbrächter, Malte; Drebes, Gerhard (2009): Marine Phytoplankton, Selected microphytoplankton species from the North Sea around Helgoland and Sylt. Schweizerbart Science Publishers, 264
Kraberg, Alexandra Claudia; Baumann, Marcus E M; Dürselen, Claus-Dieter (2010): Coastal phytoplankton. Photo guide for northern European seas. AWI Handbooks of Marine Flora and Fauna. Verlag Dr. Friedrich Pfeil, München. 204
Thielecke, Antonia; Eggers, Sarah Lena; Allhusen, Erika; Murawski, Sandra; Tippenhauer, Sandra; Rabe, Benjamin; Torres-Valdés, Sinhué: Environmental data collected during PS138 (ArcWatch1) supporting bioassays measuring carbon, nitrate and silica uptake in response to light and silicic acid interactions (EnvironmentalData) [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.991537
Thielecke, Antonia; Krause, Jeffrey W; Stefels, Jacqueline; Torres-Valdés, Sinhué; Bryan, Natasha; Fernández-Méndez, Mar: Bulk carbon, nitrate and silicic acid uptake rates of Arctic phytoplankton in response to interactive light and silicic acid gradients (CNSiUptake): [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.991536
Thielecke, Antonia; Krause, Jeffrey W; Torres-Valdés, Sinhué; Eggers, Sarah Lena; Bryan, Natasha; Narberhaus, Anna; Rabe, Benjamin; Stefels, Jacqueline; Whitehouse, Martin J; Jeon, Heejin; Fernández-Méndez, Mar (in review): Light and silicic acid co-limit late summer primary productivity across the Central Arctic Ocean.
Thielecke, Antonia; Whitehouse, Martin J; Fernández-Méndez, Mar: Single cell carbon, nitrate and silicic acid uptake rates of Arctic phytoplankton in response to light and silicic acid interactions (SIMS) [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.991544
Throndsen, Jahn; Hasle, Grethe Rytter; Tangen, Karl (2007): Phytoplankton of Norwegian coastal waters. 343
Tomas, Carmelo R (1997): Identifying Marine Phytoplankton. Elsevier, https://doi.org/10.1016/B978-0-12-693018-4.X5000-9
Torres-Valdés, Sinhué; Thielecke, Antonia: Profiles of nitrate and nitrite and silicic acid during PS138 (ArcWatch 1) to the Central Arctic Ocean (NutrientProfiles) [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.991545
Utermöhl, Hans (1958): Zur Vervollkommnung der quantitativen Phytoplankton-Methodik. Internationale Vereinigung für Theoretische und Angewandte Limnologie: Mitteilungen, 9(1), 1-38, https://doi.org/10.1080/05384680.1958.11904091
Project(s):
Funding:
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven (AWI), grant/award no. AWI_PS138_02: Biological Oceanography
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven (AWI), grant/award no. AWI_PS138_03: Physical and Chemical Oceanography
Coverage:
Median Latitude: 86.314039 * Median Longitude: 59.704672 * South-bound Latitude: 83.868300 * West-bound Longitude: -1.940574 * North-bound Latitude: 89.771984 * East-bound Longitude: 130.013152
Date/Time Start: 2023-08-10T16:38:39 * Date/Time End: 2023-09-14T12:29:24
Minimum Elevation: -4376.0 m * Maximum Elevation: -3732.0 m
Event(s):
PS138_19-1 * Latitude: 83.868300 * Longitude: 33.208806 * Date/Time: 2023-08-10T16:38:39 * Elevation: -3976.0 m * O2A Registry URI: https://registry.o2a-data.de/items/5452 * Campaign: PS138 (ArcWatch-1) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: IceStation #1, at max depth
PS138_43-1 * Latitude: 84.952384 * Longitude: 80.118858 * Date/Time: 2023-08-17T09:32:10 * Elevation: -3732.0 m * O2A Registry URI: https://registry.o2a-data.de/items/5452 * Campaign: PS138 (ArcWatch-1) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: IceStation #2, at max depth
PS138_109-1 * Latitude: 85.033489 * Longitude: 130.013152 * Date/Time: 2023-08-29T17:25:16 * Elevation: -4301.0 m * O2A Registry URI: https://registry.o2a-data.de/items/5452 * Campaign: PS138 (ArcWatch-1) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: IceStation #5, at max depth
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Thielecke, Antonia | |||
| 2 | Ice-station | Ice-station | Thielecke, Antonia | |||
| 3 | Type of study | Study type | Thielecke, Antonia | |||
| 4 | Date/time start, experiment | Date/time start exp | Thielecke, Antonia | |||
| 5 | Date/time end, experiment | Date/time end exp | Thielecke, Antonia | |||
| 6 | Treatment: silica, dissolved, added | T: Si diss add | µmol/l | Thielecke, Antonia | ||
| 7 | Treatment: light intensity | T:Io | µmol/m2/s | Thielecke, Antonia | ||
| 8 | Functional group | Functional gr | Thielecke, Antonia | Inverted light microscopy, Zeiss, IM4717 | ||
| 9 | Taxon/taxa | Taxa | Thielecke, Antonia | Inverted light microscopy, Zeiss, IM4717 | ||
| 10 | Phytoplankton | Phytopl | #/l | Thielecke, Antonia | Light microscopy (Utermöhl 1958) | |
| 11 | Phytoplankton, relative abundance | Phytopl rel abund | Thielecke, Antonia | Calculated from abundance/volume |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1650 data points