Faucher, Giulia; Haunost, Mathias; Paul, Allanah Joy; Tietz, Christiane; Riebesell, Ulf (2025): Growth response of Emiliania huxleyi to ocean alkalinity enhancement [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.977803 (DOI registration in progress)
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Published: 2025-03-27
Abstract:
We tested the sensitivity of Emiliania huxleyi, the most widespread coccolithophore species, to ocean alkalinity enhancement in laboratory culture experiments. The manipulation involved adding a NaOH (1 M) solution.
The experiment was conducted at the GEOMAR Helmholtz Centre for Ocean Research Kiel. Cells were acclimated for seven to nine generations to the respective experiment conditions. Cell density was recorded throughout the experiment with BD Accuri C6 Flow Cytometer. Samples for total particulate carbon (TPC) and particulate organic carbon (POC) were measured in an elemental analyzer (Euro EA, EuroVector). TA concentrations were determined by potentiometric titration with a Metrohm Compact 862 Titrosampler and corrected with certified reference material (A. Dickson, Scripps Institution of Oceanography, La Jolla, California). DIC concentrations were measured with an Automated Infrared Inorganic Carbon Analyzer (AIRICA) equipped with a LI-COR detector (LI-7000 CO2/H2O analyzer)
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Related to:
Faucher, Giulia; Haunost, Mathias; Paul, Allanah Joy; Tietz, Christiane; Riebesell, Ulf (2025): Growth response of Emiliania huxleyi to ocean alkalinity enhancement. Biogeosciences, 22(2), 405-415, https://doi.org/10.5194/bg-22-405-2025
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Faucher, Giulia | |||
| 2 | Year of experiment | Year exp | Faucher, Giulia | |||
| 3 | Day of experiment | DOE | day | Faucher, Giulia | ||
| 4 | Species, unique identification | Species UID | Faucher, Giulia | |||
| 5 | Alkalinity, total | AT | mmol(eq)/l | Faucher, Giulia | ||
| 6 | Alkalinity, total | AT | mmol(eq)/l | Faucher, Giulia | Metrohm Titrando titrator | mTA initial |
| 7 | Alkalinity, total | AT | mmol(eq)/l | Faucher, Giulia | Metrohm Titrando titrator | mTA end |
| 8 | Carbon, inorganic, dissolved | DIC | µmol/kg | Faucher, Giulia | LICOR Gasanalyzer (LI-7000, LI-840), both LI-COR Inc., Lincoln, NE, USA | DIC intial |
| 9 | Carbon, inorganic, dissolved | DIC | µmol/kg | Faucher, Giulia | LICOR Gasanalyzer (LI-7000, LI-840), both LI-COR Inc., Lincoln, NE, USA | DIC end |
| 10 | pH | pH | Faucher, Giulia | Calculated | calculated CO2SYS according Pierrot et al., 2006 | |
| 11 | Fugacity of carbon dioxide in seawater | fCO2w | µatm | Faucher, Giulia | Calculated | calculated CO2SYS according Pierrot et al., 2006 |
| 12 | Carbon dioxide | CO2 | µmol/kg | Faucher, Giulia | Calculated | calculated CO2SYS according Pierrot et al., 2006 |
| 13 | Bicarbonate ion | [HCO3]- | µmol/kg | Faucher, Giulia | Calculated | calculated CO2SYS according Pierrot et al., 2006 |
| 14 | Carbonate ion | [CO3]2- | µmol/kg | Faucher, Giulia | Calculated | calculated CO2SYS according Pierrot et al., 2006 |
| 15 | Aragonite saturation state | Omega Arg | Faucher, Giulia | Calculated | calculated CO2SYS according Pierrot et al., 2006 | |
| 16 | Calcite saturation state | Omega Cal | Faucher, Giulia | Calculated | calculated CO2SYS according Pierrot et al., 2006 | |
| 17 | Carbon, total, particulate | TPC | µg/l | Faucher, Giulia | Elemental analyser | |
| 18 | Carbon, organic, particulate | POC | µmol/l | Faucher, Giulia | Elemental analyser | |
| 19 | Cell density | Cells | #/ml | Faucher, Giulia | Flow cytometry system, Becton Dickinson, FACSCanto II | Cell start |
| 20 | Cell density | Cells | #/ml | Faucher, Giulia | Flow cytometry system, Becton Dickinson, FACSCanto II | Cell end |
| 21 | Day of experiment | DOE | day | Faucher, Giulia | ||
| 22 | Treatment: temperature | T:temp | °C | Faucher, Giulia | ||
| 23 | Medium | Medium | Faucher, Giulia |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
368 data points
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