Armstrong, Evelyn; Law, Cliff S (2023): Growth rate over time of Emiliania huxleyi during laboratory experiments [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.959829, In: Armstrong, E; Law, CS (2023): Physiological measurements from the long-term laboratory incubation of Emiliania huxleyi grown at present day temperature and pH conditions in subantarctic waters and projected conditions for 2100 [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.959857
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Abstract:
Emiliania huxleyi was incubated for two years (2015-11-20 to 2017-12-01) under two conditions: present-day subantarctic water during summer (11°C and pH 8.1; Now), and projected conditions for subantarctic water by 2100 (14°C and pH 7.8; Future; Law et al. 2018). The pH was reduced by 0.3 units by bubbling 10% CO₂ through the medium prior to the addition of coccolithophore cells and confirmed using a spectrophotometric method (DOE 1994). One culture of each treatment was maintained in a semi-batch style to ensure the cells remained in exponential growth, with cell concentration maintained at low levels (<80000 cells ml⁻¹) to maintain pH at the target value. The pH of the medium after cell growth was checked periodically using a spectrophotometric method (DOE 1994) and was always found to be at the target pH. The in vivo fluorescence of the cultures was measured with a Turner 10-AU fluorometer and the growth rate of the cultures was calculated from the least squares regression of the natural logarithm of in vivo fluorescence, versus time during exponential growth (Strzepek et al. 2011).
Keyword(s):
Supplement to:
Armstrong, Evelyn; Law, Cliff S (2023): Resilience of Emiliania huxleyi to future changes in subantarctic waters. PLoS ONE, 18(11), e0284415, https://doi.org/10.1371/journal.pone.0284415
References:
DOE (1994): Handbook of methods for the analysis of the various parameters of the carbon dioxide system in sea water, version 2. A. G. Dickson & C. Goyet, eds., 187 pp, hdl:10013/epic.51788.d001
Law, Cliff S; Rickard, Graham J; Mikaloff Fletcher, Sara E; Pinkerton, Matt; Behrens, Erik; Chiswell, Steve M; Currie, Kim (2018): Climate change projections for the surface ocean around New Zealand. New Zealand Journal of Marine and Freshwater Research, 52(3), 309-335, https://doi.org/10.1080/00288330.2017.1390772
Strzepek, Robert F; Maldonado, Maria T; Hunter, Keith A; Frew, Russell D; Boyd, Philip W (2011): Adaptive strategies by Southern Ocean phytoplankton to lessen iron limitation: Uptake of organically complexed iron and reduced cellular iron requirements. Limnology and Oceanography, 56(6), 1983-2002, https://doi.org/10.4319/lo.2011.56.6.1983
Funding:
Coverage:
Latitude: -45.829000 * Longitude: 171.532000
Date/Time Start: 2014-06-03T00:00:00 * Date/Time End: 2014-06-03T00:00:00
Event(s):
Emiliania_huxleyi_subantarctic * Latitude: -45.829000 * Longitude: 171.532000 * Date/Time: 2014-06-03T00:00:00 * Campaign: P140603 * Basis: Polaris II * Method/Device: Bottle, Niskin (NIS) * Comment: Original sampling of E. huxleyi between 2 -5 m depth
Comment:
The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research).
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Species, unique identification | Species UID | Armstrong, Evelyn | |||
| 2 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Armstrong, Evelyn | |||
| 3 | Species, unique identification (URI) | Species UID (URI) | Armstrong, Evelyn | |||
| 4 | Type of study | Study type | Armstrong, Evelyn | |||
| 5 | Treatment | Treat | Armstrong, Evelyn | |||
| 6 | Time in days | Time | days | Armstrong, Evelyn | 2015-11-20, experiment, start. 2017-12-01, experiment, finish. | |
| 7 | Emiliania huxleyi, growth rate | E. huxleyi µ | 1/day | Armstrong, Evelyn | Fluorometer, Turner Designs, 10-AU |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
670 data points