Schmidt, Christiane; Puerto Rueda, Diana N; Nusser, Moritz; Oakley, Clinton A; Pochon, Xavier; Stuhr, Marleen; Raposo, Débora; Davy, Simon K (2025): Menthol-DCMU bleaching experiments on two foraminifera species, Amphistegina lobifera and Sorites orbiculus [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.974618 (DOI registration in progress)
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Published: 2025-03-18
Abstract:
This dataset documents experiments conducted on two foraminifera species, Amphistegina lobifera and Sorites orbiculus. Samples of Sorites orbiculus were collected from Eilat, Israel, and Amphistegina lobifera from the Mediterranean Sea, Sicily. Experiments were conducted from October to December 2019 at University of Victoria, Wellington in New Zealand. The aim of these experiments was to establish a rapid bleaching methodology to produce aposymbiotic hosts without compromising survivorship. Three independent experiments were carried out. The Menthol Concentration Comparison (MCC) experiment tested menthol concentrations (0.05 - 0.25 mmol L⁻¹) to determine an effective non-lethal dose for both species. The Bleaching Ecophysiology Assessment (BEA) used a single concentration (0.19 mmol L⁻¹) to evaluate growth, motility, and mortality with larger sample sizes. The 33-day High Concentration Trial (HCT) assessed motility under higher menthol concentrations at incremental steps (0.35, 0.4, 0.45, 0.50, 0.55, and 0.6 mmol L⁻¹). Data were collected using epifluorescence microscopy, growth assessments, survivorship assessments using photographs and pseudopodial movement, and motility analysis. The results provide a robust methodology for rapidly rearing aposymbiotic foraminifera, ensuring methodological reproducibility and minimizing lethality.
Keyword(s):
Related to:
Schmidt, Christiane; Puerto Rueda, Diana N; Nusser, Moritz; Oakley, Clinton A; Pochon, Xavier; Stuhr, Marleen; Raposo, Débora; Davy, Simon K (accepted): A novel menthol-DCMU bleaching method for foraminifera: Generating aposymbiotic hosts for symbiosis research. biorxiv, Symbiosis, 2024.12.09.627035v1, https://doi.org/10.1101/2024.12.09.627035
Funding:
Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. 444059848: SYMBIOAID: the role of diatom endosymbionts on the adaptive potenital of benthic foraminifera to climate change
Coverage:
Median Latitude: 33.094266 * Median Longitude: 25.027883 * South-bound Latitude: 29.501866 * West-bound Longitude: 15.138278 * North-bound Latitude: 36.686667 * East-bound Longitude: 34.917488
Date/Time Start: 2019-10-29T00:00:00 * Date/Time End: 2019-12-19T00:00:00
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
5 datasets
Download Data
Datasets listed in this bundled publication
- Schmidt, C; Puerto Rueda, DN; Nusser, M et al. (2025): File names of the images and videos recorded at the microscope from the Bleaching Ecophysiology Assessment experiment at final observations. https://doi.pangaea.de/10.1594/PANGAEA.974619
- Schmidt, C; Puerto Rueda, DN; Nusser, M et al. (2025): Growth and mortality data of the foraminifera and the file names of images. https://doi.pangaea.de/10.1594/PANGAEA.974620
- Schmidt, C; Puerto Rueda, DN; Nusser, M et al. (2025): Motility of Amphistegina lobifera throughout the 4-week BEA experiment. https://doi.pangaea.de/10.1594/PANGAEA.974621
- Schmidt, C; Puerto Rueda, DN; Nusser, M et al. (2025): Motility of Amphistegina lobifera throughout the 6-week BEA experiment. https://doi.pangaea.de/10.1594/PANGAEA.974622
- Schmidt, C; Puerto Rueda, DN; Nusser, M et al. (2025): Motility of Amphistegina lobifera throughout the High Concentration Trial HCT experiment. https://doi.pangaea.de/10.1594/PANGAEA.974623
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