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Cardoso, Isabel; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C: Number of germinated individuals that originated from protoplasts from Ulva lacinulata and Ulva compressa [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972135 (dataset in review), In: Cardoso, I et al.: Naturally occurring protoplasts in two Ulva spp. - Disc sizes from discs grown from protoplasts, after 35 days of germination, under culture conditions [dataset bundled publication]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.972125 (dataset in review)

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Abstract:
This dataset presents the number of germinated individuals that originated from protoplasts from Ulva lacinulata and Ulva compressa. The individuals are separated into different morphologies (unattached discs and unattached germlings). Unattached discs are also separated into the color they presented at the end of the experiment to indicate fertility. Green discs: not fertile; Brown discs: fertile; White discs: dead discs after gamete release. This allowed to calculate the germination rate, morphology ratios and percentage of fertility.
Keyword(s):
degradation; Laboratory experiment; pincuschion; protoplasts; Reproduction; Ulva; Ulva compressa; Ulva lacinulata
Related to:
Cardoso, Isabel; et al. (in review): Naturally occurring protoplasts in Ulva spp. reveal a previously underestimated proliferation process.
Funding:
Federal Ministry of Food and Agriculture (BMEL), grant/award no. 281DL02B20: Verbundprojekt: Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel (MakPakScaleUp) - Teilprojekt B
Swedish Research Council (VR), grant/award no. 2022-00331_Formas: A manual for the use of sustainable marine resources – Definition of the cultivation potential and commercially relevant compounds of Sea Lettuces (Ulva spp.) along the Swedish coastline
Coverage:
Median Latitude: 49.642771 * Median Longitude: 3.449373 * South-bound Latitude: 39.394977 * West-bound Longitude: -9.213583 * North-bound Latitude: 53.741889 * East-bound Longitude: 8.514556
Date/Time Start: 2021-01-04T12:00:00 * Date/Time End: 2023-07-24T10:00:00
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 0 m
Event(s):
Ulva_Dorum * Latitude: 53.741889 * Longitude: 8.514556 * Date/Time: 2023-07-24T10:00:00 * Method/Device: Biological sample (BIOS) * Comment: Species were collected during low tide in a Lagoon were there was no water (at that specific time)
Ulva_NE-Atlantic * Latitude: 39.394977 * Longitude: -9.213583 * Date/Time: 2021-01-04T12:00:00 * Method/Device: Biological sample (BIOS) * Comment: Species were collected during low tide in a Lagoon were there was no water (at that specific time)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventHofmann, Laurie COriginal Sample collection
2DATE/TIMEDate/TimeHofmann, Laurie CGeocode – Original Sample collection
3LATITUDELatitudeHofmann, Laurie CGoogle MapsGeocode – Original Sample collection
4LONGITUDELongitudeHofmann, Laurie CGoogle MapsGeocode – Original Sample collection
5DEPTH, waterDepth watermHofmann, Laurie CGeocode – The species were collected by the principal investigator during low tide in mudflat areas were there was no water (at that specific time)
6Type of studyStudy typeHofmann, Laurie C
7LaboratoryLabHofmann, Laurie C
8Latitude 2Latitude 2Hofmann, Laurie CGoogle Mapsof laboratory
9Longitude 2Longitude 2Hofmann, Laurie CGoogle Mapsof laboratory
10Date/time start, experimentDate/time start expHofmann, Laurie C
11Date/time end, experimentDate/time end expHofmann, Laurie C
12Day of experimentDOEdayHofmann, Laurie Ctime of measurement
13SpeciesSpeciesHofmann, Laurie C
14Species, unique identification (Semantic URI)Species UID (Semantic URI)Hofmann, Laurie C
15Species, unique identification (URI)Species UID (URI)Hofmann, Laurie C
16CollectionCollectionHofmann, Laurie CCollection Number
17Accession number, geneticsAccess no genSteinhagen, SophieGenBank NCBIMolecular analysis with the Invisorb Spin Plant Mini Kit (Stratec); gel electrophoresis and subsequently purified using the QIAquick PCR Purification Kit (Qiagen); Sanger sequencing was performed by Eurofins Genomics (Konstanz, Germany); Sequence reads were assembled in sequencher (version 4.1.4, Gene Codes Corporation). BLAST function in GenBank, identifications via the specimens' tufA sequences were made.
18ReplicateReplHofmann, Laurie C
19ReplicateReplHofmann, Laurie CSubreplicate
20Treatment: temperatureT:temp°CHofmann, Laurie CDigital thermometer
21Treatment: salinityT:salHofmann, Laurie CRefractometerPSU
22Treatment: light conditionT:lightHofmann, Laurie CDay length: h, light:dark
23Treatment: light intensityT:Ioµmol/m2/sHofmann, Laurie CLight meter, LI-COR Biosciences GmbH, LI-250A
24Treatment: nutrientsT:nutrientsHofmann, Laurie CNutrient Source; Provasoli's enriched seawater (PES), half-strength (Provasoli 1986; Tatewaki 1966)
25Treatment: nutrientsT:nutrientsHofmann, Laurie CNutrient Source - Concentration [mL L-1]
26Incubation volumeInc volmlHofmann, Laurie CPetri dish - Volume
27ProtoplastsProtoplasts#Hofmann, Laurie C# Protoplast inoculated in each petri dish
28ProtoplastsProtoplasts#Hofmann, Laurie C# Regeneration: Number of protoplasts that regenerated
29ProtoplastsProtoplasts#Hofmann, Laurie CGermination - Morphology - UG: Number of protoplasts that developed into an unattached germling morphology
30ProtoplastsProtoplasts#Hofmann, Laurie CGermination - Morphology - UD: Number of protoplasts that developed into an unattached disc morphology
31ProtoplastsProtoplasts#Hofmann, Laurie CGermination - UD Color - Green: Number of protoplasts that developed into an unattached disc morphology and presented a green color
32ProtoplastsProtoplasts#Hofmann, Laurie CGermination - UD Color - Brown: Number of protoplasts that developed into an unattached disc morphology and presented a brown color (fertile)
33ProtoplastsProtoplasts#Hofmann, Laurie CGermination - UD Color - White: Number of protoplasts that developed into an unattached disc morphology and presented a white color (dead)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2208 data points

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